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Search Results: 1 - 10 of 32710 matches for " Wen-Hwa Ko "
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Food Sanitation Knowledge, Attitude, and Behavior for the University Restaurants Employees  [PDF]
Wen-Hwa Ko
Food and Nutrition Sciences (FNS) , 2011, DOI: 10.4236/fns.2011.27102
Abstract: The purpose of this study was to discuss the food sanitation knowledge, attitude, and behavior for the employees of university restaurants, and furthermore, to explain the interrelations of these various were occurring at school. A questionnaire survey procedure was used. Each two constructs of food sanitation attitude and behavior were analyzed by factor analysis. Data was analyzed by description, Pearson’s correlation and multiple regression analysis. The correctness rate toward the whole sanitation knowledge of trials was 70%. The overall attitude toward food sanitation was prone to positive and the attitude of employee self-responsibility was superior to the attitude of food sanitation practice. The sanitary guiding behavior was better than the sanitary habit behavior; and there was a significant relationship indicated through Pearson correlation analysis among three various. The sanitation knowledge and sanitation attitude showed a 42.6% predictive power to behavior, the attitude was mediated between knowledge and behavior. The university restaurant employees shared a more pessimistic view toward the benefits of training and the institutions could establish a committee for monitoring food nutrition and sanitation. It provides valuable information for development employees training while seeking to raise school restaurant food safety levels.
Foodservice Quality: Identifying Perception Indicators of Foodservice Quality for Hospitality Students  [PDF]
Wen-Hwa Ko, Li-Jung Su
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.52017

This study uses an expert perspective to identify indicators of foodservice quality as perceived by hospitality students. A 3-round modified Delphi method and an expert panel comprising culinary arts instructors, master chefs with >10 years of experience, and senior foodservice industry executive managers were used to define and identify these indicators. The developed foodservice quality framework includes 2 categories, the product and customer, which comprise 57 indicators in 6 dimensions. The results of this study provide a clear direction for further studies and applications for hospitality professionals, educators, and students.

Loss of ZBRK1 Contributes to the Increase of KAP1 and Promotes KAP1-Mediated Metastasis and Invasion in Cervical Cancer
Li-Fang Lin, Chien-Feng Li, Wei-Jan Wang, Wen-Ming Yang, Dennis Ding-Hwa Wang, Wen-Chang Chang, Wen-Hwa Lee, Ju-Ming Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073033
Abstract: ZBRK1, a zinc finger protein that interacts with breast cancer 1 (BRCA1) and KRAB-ZFP-associated protein 1 (KAP1), has been suggested to serve as a tumor suppressor via repression of tumor metastasis/invasion. To date, the detailed molecular mechanisms for how BRCA1 and KAP1 participate in ZBRK1-mediated transcriptional repression, metastasis and invasion as well as the associated clinical relevance remain unclear. In this study, we demonstrated that both the N- and C-terminal domains of ZBRK1 are important for inhibiting cell proliferation and anchorage-independent growth in cervical cancer. Specifically, the N-terminal KRAB domain of ZBRK1 displayed a more crucial role in inhibiting metastasis and invasion through modulation of KAP1 function in a transcriptionally dependent manner. The loss of ZBRK1 results in an increase of KAP1 expression, which enhanced migration and invasion of cervical cancer cells both the in vitro and in vivo. Moreover, an inverse correlation of expression levels was observed between ZBRK1 and KAP1 following tumor progression from in situ carcinoma to invasive/metastatic cervical cancer specimens. Taken together, the current results indicate that a loss of ZBRK1 contributes to the increased expression of KAP1, potentiating its role to enhance metastasis and invasion.
Breast Cancer Cells Induce Cancer-Associated Fibroblasts to Secrete Hepatocyte Growth Factor to Enhance Breast Tumorigenesis
Shiaw-Wei Tyan,Wen-Hung Kuo,Chun-Kai Huang,Chi-Chun Pan,Jin-Yuh Shew,King-Jen Chang,Eva Y.-H. P. Lee,Wen-Hwa Lee
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0015313
Abstract: It has been well documented that microenvironment consisting of stroma affects breast cancer progression. However, the mechanisms by which cancer cells and fibroblasts, the major cell type in stroma, interact with each other during tumor development remains to be elucidated. Here, we show that the human cancer-associated fibroblasts (CAFs) had higher activity in enhancing breast tumorigenecity compared to the normal tissue-associated fibroblasts (NAFs) isolated from the same patients. The expression level of hepatocyte growth factor (HGF) in these fibroblasts was positively correlated with their ability to enhance breast tumorigenesis in mice. Deprivation of HGF using a neutralizing antibody reduced CAF-mediated colony formation of human breast cancer cells, indicating that CAFs enhanced cancer cell colony formation mainly through HGF secretion. Co-culture with human breast cancer MDA-MB-468 cells in a transwell system enhanced NAFs to secret HGF as well as promote tumorigenecity. The newly gained ability of these “educated” NAFs became irreversible after continuing this process till fourth passage. These results suggested that breast cancer cells could alter the nature of its surrounding fibroblasts to secrete HGF to support its own progression through paracrine signaling.
Multiple Lineages of Human Breast Cancer Stem/Progenitor Cells Identified by Profiling with Stem Cell Markers
Wendy W. Hwang-Verslues,Wen-Hung Kuo,Po-Hao Chang,Chi-Chun Pan,Hsing-Hui Wang,Sheng-Ta Tsai,Yung-Ming Jeng,Jin-Yu Shew,John T. Kung,Chung-Hsuan Chen,Eva Y.-H. P. Lee,King-Jen Chang,Wen-Hwa Lee
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008377
Abstract: Heterogeneity of cancer stem/progenitor cells that give rise to different forms of cancer has been well demonstrated for leukemia. However, this fundamental concept has yet to be established for solid tumors including breast cancer. In this communication, we analyzed solid tumor cancer stem cell markers in human breast cancer cell lines and primary specimens using flow cytometry. The stem/progenitor cell properties of different marker expressing-cell populations were further assessed by in vitro soft agar colony formation assay and the ability to form tumors in NOD/SCID mice. We found that the expression of stem cell markers varied greatly among breast cancer cell lines. In MDA-MB-231 cells, PROCR and ESA, instead of the widely used breast cancer stem cell markers CD44+/CD24-/low and ALDH, could be used to highly enrich cancer stem/progenitor cell populations which exhibited the ability to self renew and divide asymmetrically. Furthermore, the PROCR+/ESA+ cells expressed epithelial-mesenchymal transition markers. PROCR could also be used to enrich cells with colony forming ability from MB-361 cells. Moreover, consistent with the marker profiling using cell lines, the expression of stem cell markers differed greatly among primary tumors. There was an association between metastasis status and a high prevalence of certain markers including CD44+/CD24?/low, ESA+, CD133+, CXCR4+ and PROCR+ in primary tumor cells. Taken together, these results suggest that similar to leukemia, several stem/progenitor cell-like subpopulations can exist in breast cancer.
Breast Cancer Cells Induce Stromal Fibroblasts to Secrete ADAMTS1 for Cancer Invasion through an Epigenetic Change
Shiaw-Wei Tyan, Chih-Hung Hsu, Kai-Lin Peng, Chun-Chin Chen, Wen-Hung Kuo, Eva Y.-H. P. Lee, Jin-Yuh Shew, King-Jen Chang, Li-Jung Juan, Wen-Hwa Lee
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035128
Abstract: Microenvironment plays an important role in cancer development. We have reported that the cancer-associated stromal cells exhibit phenotypic and functional changes compared to stromal cells neighboring to normal tissues. However, the molecular mechanisms as well as the maintenance of these changes remain elusive. Here we showed that through co-culture with breast cancer cells for at least three to four passages, breast normal tissue-associated fibroblasts (NAFs) gained persistent activity for promoting cancer cell invasion, partly via up-regulating ADAM metallopeptidase with thrombospondin type 1 motif, 1 (ADAMTS1). Furthermore, we demonstrated that the DNA methylation pattern in the ADAMTS1 promoter has no alteration. Instead, the loss of EZH2 binding to the ADAMTS1 promoter and the resulting decrease of promoter-associated histone H3K27 methylation may account for the up-regulation of ADAMTS1. Importantly, the lack of EZH2 binding and the H3K27 methylation on the ADAMTS1 promoter were sustained in cancer cell-precocultured NAFs after removal of cancer cells. These results suggest that cancer cells are capable of inducing stromal fibroblasts to secrete ADAMTS1 persistently for their invasion and the effect is epigenetically inheritable.
Open Access Target Validation Is a More Efficient Way to Accelerate Drug Discovery
Wen Hwa Lee
PLOS Biology , 2015, DOI: 10.1371/journal.pbio.1002164
Abstract: There is a scarcity of novel treatments to address many unmet medical needs. Industry and academia are finally coming to terms with the fact that the prevalent models and incentives for innovation in early stage drug discovery are failing to promote progress quickly enough. Here we will examine how an open model of precompetitive public–private research partnership is enabling efficient derisking and acceleration in the early stages of drug discovery, whilst also widening the range of communities participating in the process, such as patient and disease foundations.
Calpain/SHP-1 Interaction by Honokiol Dampening Peritoneal Dissemination of Gastric Cancer in nu/nu Mice
Shing Hwa Liu,Keh Bin Wang,Keng Hsin Lan,Wen Jane Lee,Hung Chuan Pan,Sheng Mao Wu,Yen Chun Peng,Yi Ching Chen,Chin Chang Shen,Hsu Chen Cheng,Ko Kaung Liao,Meei Ling Sheu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0043711
Abstract: Honokiol, a small-molecular weight natural product, has previously been reported to activate apoptosis and inhibit gastric tumorigenesis. Whether honokiol inhibits the angiogenesis and metastasis of gastric cancer cells remains unknown.
A Grid-Based Distributed Event Detection Scheme for Wireless Sensor Networks
Ja Won Ko,Yoon-Hwa Choi
Sensors , 2011, DOI: 10.3390/s111110048
Abstract: This paper presents a grid-based distributed event detection scheme for wireless sensor networks. The network is divided into square-shaped grids of predefined grid size, where sensor nodes in each grid form a cluster with a cluster head. Event detection at each grid alone based on the readings of its member nodes is limited in event detection performance, especially for a small event region compared to the grid size. To improve the performance, each grid is further divided into 2 × 2 sub-grids of equal size. The decision on an event is made by finding a square region of 2 × 2 sub-grids, not necessarily in the same grid, that passed a predefined threshold. This process is conducted at each cluster head in a distributed manner by inter-cluster communications. Event detection is initiated when a cluster head receives an alarm from its member nodes. The cluster-head communicates with its neighboring cluster heads to exchange the number of nodes reporting an alarm. The threshold for event detection can be dynamically adjusted to reflect the number of sensor nodes in a grid and event size, if known. High event detection accuracy is achieved with a relatively low threshold without sacrificing false alarm rate by filtering most errors due to transient faults and isolating nodes with permanent faults. Experimental results show that the proposed scheme can achieve high detection accuracy, while maintaining low false alarm rate.
Uremic Pruritus, Dialysis Adequacy, and Metabolic Profiles in Hemodialysis Patients: A Prospective 5-Year Cohort Study
Mei-Ju Ko, Hon-Yen Wu, Hung-Yuan Chen, Yen-Ling Chiu, Shih-Ping Hsu, Mei-Fen Pai, Ju-YehYang, Chun-Fu Lai, Hui-Min Lu, Shu-Chen Huang, Shao-Yu Yang, Su-Yin Wen, Hsien-Ching Chiu, Fu-Chang Hu, Yu-Sen Peng, Shiou-Hwa Jee
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071404
Abstract: Background Uremic pruritus is a common and intractable symptom in patients on chronic hemodialysis, but factors associated with the severity of pruritus remain unclear. This study aimed to explore the associations of metabolic factors and dialysis adequacy with the aggravation of pruritus. Methods We conducted a 5-year prospective cohort study on patients with maintenance hemodialysis. A visual analogue scale (VAS) was used to assess the intensity of pruritus. Patient demographic and clinical characteristics, laboratory parameters, dialysis adequacy (assessed by Kt/V), and pruritus intensity were recorded at baseline and follow-up. Change score analysis of the difference score of VAS between baseline and follow-up was performed using multiple linear regression models. The optimal threshold of Kt/V, which is associated with the aggravation of uremic pruritus, was determined by generalized additive models and receiver operating characteristic analysis. Results A total of 111 patients completed the study. Linear regression analysis showed that lower Kt/V and use of low-flux dialyzer were significantly associated with the aggravation of pruritus after adjusting for the baseline pruritus intensity and a variety of confounding factors. The optimal threshold value of Kt/V for pruritus was 1.5 suggested by both generalized additive models and receiver operating characteristic analysis. Conclusions Hemodialysis with the target of Kt/V ≥1.5 and use of high-flux dialyzer may reduce the intensity of pruritus in patients on chronic hemodialysis. Further clinical trials are required to determine the optimal dialysis dose and regimen for uremic pruritus.
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