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Search Results: 1 - 10 of 127118 matches for " Weiyun Li? "
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Electronic Structures and Magnetic Properties of Co-Adsorbed Monolayer WS2  [PDF]
Weiyun Xu
Journal of Materials Science and Chemical Engineering (MSCE) , 2016, DOI: 10.4236/msce.2016.410004
Abstract: Using the first-principles density functional theory (DFT) calculations, we study the effects of Co adatom on the electronic and magnetic properties of monolayer WS2. The calculations show that, for the high symmetry adsorption sites (Tw, H and Ts) on the surface of monolayer WS2, Co atom prefers Tw site. The p-d hybridization mechanism for the magnetism results in the splitting of the energy levels near the Fermi energy. A total magnetic moment of ~1.0 μB is found in WS2 system with one Co adsorbed and local magnetic moment which mainly focuses on the adsorption site. For Tw adsorption position, we further investigate the formation energy of the ferromagnetic (FM) and the antiferromagnetic (AFM) states under different monolayer coverage (ML) of Co atoms. The FM configurations are relatively stable at 0.50 ML and 1.0 ML. The local density of states (LDOS) and band calculations reveal that both of them present half-metal ferromagnetic materials’ property, which are the important preparation materials for spintronic devices.
Assessing Pre-Service Teachers’ Soccer Offensive Game Performance Ability  [PDF]
Weiyun Chen, Kristin Hendricks
Advances in Physical Education (APE) , 2012, DOI: 10.4236/ape.2012.23021
Abstract: This study aimed to design and validate the Soccer Offensive Game Performance Instrument (SOGPI) that assessed pre-service teachers’ offensive game abilities in soccer. One investigator videotaped two 10-minute 3 vs. 3 soccer games played by twelve pre-service teachers at the end of a soccer unit. Two investigators independently coded 480 offensive game behaviors with the SOGPI. The results of t-tests indicated that the SOGPI was a valid instrument to differentiate the players’ overall game performance and individual game components between the novice and the experience groups. The results of the inter-rater reliability indicated that the SOGPI was a reliable instrument to assess pre-service teachers’ offensive game abilities in soccer.
Effect of Complex Lactic Acid Bacteria on Silage Quality and In vitro Dry Matter Digestibility of Corn Straw
Yongkai Li,Chengqun Yu,Weiyun Zhu,Tao Shao
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.1395.1399
Abstract: The study was carried out to evaluate the effect of complex lactic acid bacteria on the silage fermentation quality of corn straw and the silage in vitro dry matter digestibility. In the first experiment, the complex lactic acid bacteria inoculants was applied at 3.0x105 cfu g-1 consisted of 1.0x105 cfu g-1 Enterococcus faecalis TBT608 and 2.0x105 cfu g-1 Lactobacillus plantram TBT717. Uninoculated silage served as control. After 150 days of storage, the treatment silage had significantly (p<0.05) lower pH values, butyric acid, acetic acid, propionic acid, ammonia N concentration and had significantly (p<0.05) higher contents of lactic acid than control silage. In the second experiment, In Vitro Dry Matter Digestibility (IVDMD) was determined through fermentation, inoculated with rumen fluid of wethers in various combinations with soluble starch. The soluble starch represented a concentrate feed, silage represented feeding sole roughage. The IVDMD was determined at 24 and 48 h after incubation. Silage without inoculated LAB served as control for each feed combination. The results indicate that LAB applied at ensiling increased the IVDMD of the corn straw silage. These results confirmed that application of the complex lactic acid bacteria improved fermentation quality and in vitro dry matter digestibility of the corn straw silage.
The Microbial Community in the Feces of the White Rhinoceros (Ceratotherium simum) as Determined by Barcoded Pyrosequencing Analysis
Gaorui Bian, Li Ma, Yong Su, Weiyun Zhu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070103
Abstract: As a non-ruminant herbivore, the white rhinoceros has the ability to utilize fibrous plant matter through microbial fermentation in the hindgut. So far, there has been no report using molecular techniques to study the gut microbiota of the white rhinoceros. We used barcoded pyrosequencing to characterize 105,651 sequences of 16S rRNA genes obtained from fecal samples from five white rhinoceroses. Results showed that Firmicutes and Bacteroidetes were the predominant phyla in the samples, which were comprised largely of unclassified bacteria. The microbiota of one animal treated with drug therapy differed from those in other healthy animals, and was dominated by Aerococcus -related bacteria. The core microbiota in the healthy rhinoceros were dominated by phyla Firmicutes and Bacteroidetes, represented by the Ruminococcaceae, Lachnospiraceae, Rikenellaceae and Prevotellaceae families. The present work provides a phylogenetic framework for understanding the complex microbial community of the rhinoceros; however, further studies are required to link the distinctive microbiota with their digestive role in the hindgut of the white rhinoceros.
Macrophage Depletion Impairs Corneal Wound Healing after Autologous Transplantation in Mice
Suxia Li, Bin Li, Haoran Jiang, Yao Wang, Mingli Qu, Haoyun Duan, Qingjun Zhou, Weiyun Shi
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061799
Abstract: Purpose Macrophages have been shown to play a critical role in the wound healing process. In the present study, the role of macrophages in wound healing after autologous corneal transplantation was investigated by depleting local infiltrated macrophages. Methods Autologous corneal transplantation model was used to induce wound repair in Balb/c mice. Macrophages were depleted by sub-conjunctival injections of clodronate-containing liposomes (Cl2MDP-LIP). The presence of CD11b+ F4/80+ macrophages, α-smooth muscle actin+ (α-SMA+) myofibroblasts, CD31+ vascular endothelial cells and NG2+ pericytes was examined by immunohistochemical and corneal whole-mount staining 14 days after penetrating keratoplasty. Peritoneal macrophages were isolated from Balb/c mice and transfused into conjunctiva to examine the recovery role of macrophages depletion on wound healing after autologous corneal transplantation. Results Sub-conjunctival Cl2MDP-LIP injection significantly depleted the corneal resident phagocytes and infiltrated macrophages into corneal stroma. Compared with the mice injected with PBS-liposome, the Cl2MDP-LIP-injected mice showed few inflammatory cells, irregularly distributed extracellular matrix, ingrowth of corneal epithelium into stroma, and even the detachment of donor cornea from recipient. Moreover, the number of macrophages, myofibroblasts, endothelial cells and pericytes was also decreased in the junction area between the donor and recipient cornea in macrophage-depleted mice. Peritoneal macrophages transfusion recovered the defect of corneal wound healing caused by macrophages depletion. Conclusions Macrophage depletion significantly impairs wound healing after autologous corneal transplantation through at least partially impacting on angiogenesis and wound closure.
Establishment of an in vitro cell culture system transfected by full-length HCV cDNA genome
Xiangjie Yao,Jia Guo,Congyi Zheng,Chengxiang Fang,Sanfu Qu,Zhenqiu Chen,Zhonghong Li,Xinqiang Li,Weiyun Li
Chinese Science Bulletin , 2004, DOI: 10.1360/03wc0369
Abstract: A new cell culture system expressing the entire HCV genome has been established in vitro. To initiate transcription of HCV RNA, HeLa cells were transfected with a recombinant plasmid containing full-length HCV cDNA genome by using lipofectamine 2000, followed by infection with recombinant vaccinia virus vTF7-3 containing the T7 RNA polymerase gene. Synthesis of positive-strand HCV RNA could be detected in the transfected cells by RT-PCR. Western blot analysis revealed that HCV structural and nonstructural proteins were correctly processed. In transfected HeLa cells 47 nm virus-like particles were assembled, which could be recognized by anti-HCV E2 antibodies. The titer of HCV was 107 copies/mL in our cell culture system, which was significantly higher than that of infected patients’ sera and that from all reported cell culture systems. Supernatant from transfected HeLa cells were infectious to Huh7 cells and the titer of HCV was 106 copies/mL. Moreover, negative-strand RNA of HCV in Huh7 cells could be detected by using strand-specific RT-PCR, which demonstrated that replication of HCV occurred in the permissive cell lines.
The Immune Adaptor ADAP Regulates Reciprocal TGF-β1-Integrin Crosstalk to Protect from Influenza Virus Infection
Chunyang Li,Shaozhuo Jiao?,Guojun Wang?,Yunzhen Gao?,Chang Liu?,Xijun He?,Chi Zhang?,Jun Xiao?,Weiyun Li,Guoquan Zhang
PLOS Pathogens , 2015, DOI: 10.1371/journal.ppat.1004824
Abstract: Highly pathogenic avian influenza virus (HPAI, such as H5N1) infection causes severe cytokine storm and fatal respiratory immunopathogenesis in human and animal. Although TGF-β1 and the integrin CD103 in CD8+ T cells play protective roles in H5N1 virus infection, it is not fully understood which key signaling proteins control the TGF-β1-integrin crosstalk in CD8+ T cells to protect from H5N1 virus infection. This study showed that ADAP (Adhesion and Degranulation-promoting Adapter Protein) formed a complex with TRAF6 and TAK1 in CD8+ T cells, and activated SMAD3 to increase autocrine TGF-β1 production. Further, TGF-β1 induced CD103 expression via an ADAP-, TRAF6- and SMAD3-dependent manner. In response to influenza virus infection (i.e. H5N1 or H1N1), lung infiltrating ADAP-/- CD8+ T cells significantly reduced the expression levels of TGF-β1, CD103 and VLA-1. ADAP-/- mice as well as Rag1-/- mice receiving ADAP-/- T cells enhanced mortality with significant higher levels of inflammatory cytokines and chemokines in lungs. Together, we have demonstrated that ADAP regulates the positive feedback loop of TGF-β1 production and TGF-β1-induced CD103 expression in CD8+ T cells via the TβRI-TRAF6-TAK1-SMAD3 pathway and protects from influenza virus infection. It is critical to further explore whether the SNP polymorphisms located in human ADAP gene are associated with disease susceptibility in response to influenza virus infection.
Identification, Characterization and Application of a G-Quadruplex Structured DNA Aptamer against Cancer Biomarker Protein Anterior Gradient Homolog 2
Jie Wu, Chi Wang, Xilan Li, Yanling Song, Wei Wang, Cong Li, Jia Hu, Zhi Zhu, Jiuxing Li, Weiyun Zhang, Zhongxian Lu, Chaoyong James Yang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0046393
Abstract: Background Anterior gradient homolog 2 (AGR2) is a functional protein with critical roles in a diverse range of biological systems, including vertebrate tissue development, inflammatory tissue injury responses, and cancer progression. Clinical studies have shown that the AGR2 protein is overexpressed in a wide range of human cancers, including carcinomas of the esophagus, pancreas, breast, prostate, and lung, making the protein as a potential cancer biomarker. However, the general biochemical functions of AGR2 in human cells remain undefined, and the signaling mechanisms that drive AGR2 to inhibit p53 are still not clearly illustrated. Therefore, it is of great interest to develop molecular probes specifically recognizing AGR2 for its detection and for the elucidation of AGR2-associated molecular mechanism. Methodology/Principal Findings Through a bead-based and flow cytometry monitored SELEX technology, we have identified a group of DNA aptamers that can specifically bind to AGR2 with Kd values in the nanomolar range after 14 rounds of selections. Aptamer C14B was chosen to further study, due to its high binding affinity and specificity. The optimized and shortened C14B1 has special G-rich characteristics, and the G-rich region of this binding motif was further characterized to reveal an intramolecular parallel G-quadruplex by CD spectroscopy and UV spectroscopy. Our experiments confirmed that the stability of the G-quadruplex structure was strongly dependent on the nature of the monovalent ions and the formation of G-quadruplex structure was also important for the binding capacity of C14B1 to the target. Furthermore, we have designed a kind of allosteric molecule beacon (aMB) probe for selective and sensitive detection of AGR2. Conclusion/Significance In this work, we have developed new aptamer probes for specific recognition of the AGR2. Structural study have identified that the binding motif of aptamer is an intramolecular parallel G-quadruplex structure and its structure and binding affinity are strongly dependent on the nature of the monovalent ion. Furthermore, with our design of AGR2-aMB, AGR2 could be sensitively and selectively detected. This aptamer probe has great potential to serve as a useful tool for early diagnosis and prognosis of cancer and for fundamental research to elucidate the biochemical functions of AGR2.
Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity
Dezhi Kang, Jiangjie Wang, Weiyun Zhang, Yanling Song, Xilan Li, Yuan Zou, Mingtao Zhu, Zhi Zhu, Fuyong Chen, Chaoyong James Yang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042731
Abstract: Background Glioblastoma is the most common and most lethal form of brain tumor in human. Unfortunately, there is still no effective therapy to this fatal disease and the median survival is generally less than one year from the time of diagnosis. Discovery of ligands that can bind specifically to this type of tumor cells will be of great significance to develop early molecular imaging, targeted delivery and guided surgery methods to battle this type of brain tumor. Methodology/Principal Findings We discovered two target-specific aptamers named GBM128 and GBM131 against cultured human glioblastoma cell line U118-MG after 30 rounds selection by a method called cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX). These two aptamers have high affinity and specificity against target glioblastoma cells. They neither recognize normal astraglial cells, nor do they recognize other normal and cancer cell lines tested. Clinical tissues were also tested and the results showed that these two aptamers can bind to different clinical glioma tissues but not normal brain tissues. More importantly, binding affinity and selectivity of these two aptamers were retained in complicated biological environment. Conclusion/Significance The selected aptamers could be used to identify specific glioblastoma biomarkers. Methods of molecular imaging, targeted drug delivery, ligand guided surgery can be further developed based on these ligands for early detection, targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma.
RANGES OF PERTURBED MAXIMAL MONOTONE OPERATORS IN BANACH SPACES
Banach 空间中极大单调算子扰动的值域

Ren Weiyun,
任卫云

系统科学与数学 , 2005,
Abstract: Let $X$ be a real Banach space, $T:D(T)\subset X\rightarrow 2^{X^{*}}$ be a maximal monotone operator , $C:D(T)\subset X\rightarrow X^{*}$ be bounded (but need not to be continuous) and $C(T+J)^{-1}$ be a compact operator. Under the above conditions, by adding certain boundary and making use of Leray-Schauder degree theory, in this paper we study the solvability of the following inclusions: $0\in\overline{(T+C)(D(T)\cap B_Q(0))},\hspace{1mm} 0\in(T+C)(D(T)\cap B_Q(0))$; and $S\subset\overline{R(T+C)}$, int$S$$\subset$int$ R(T+C)$ (where $S\subset X^{*}$); and $B+D\subset\overline{R(T+C)}$, int$(B+D)$$\subset$int$ {R(T+C)}$ (where $B\subset X^{*}$ and $D\subset X^{*}$). Based on this, we derive some new conclusions.
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