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Search Results: 1 - 10 of 7628 matches for " Weixing Shan "
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Shan Weixing Li Junyan,

微生物学通报 , 1993,
Abstract: 本文报道棉铃疫病菌即苎麻疫霉菌(Phytophthora boehmeriae)孢子囊的离体生产及游动孢子悬液的稀释方法。将疫病菌菌饼置于马铃薯葡萄糖培养液(PDL)或菜豆葡萄糖培养液(BDL)中,24C条件下暗培养48—72小时,然后将菌丝块在20-22℃条件下用矿质盐溶液(MSS)更换漂洗(4次)培养10-12小时并辅以光照(日光灯);最后除去MSS,继续光照培养12小时即可得到大量孢子囊。黑暗条件可抑制孢子囊的形成而促进卵孢子的产生。培养液采用BDL优于PDL,除了产孢量更高外,突出的
Production of dsRNA Sequences in the Host Plant Is Not Sufficient to Initiate Gene Silencing in the Colonizing Oomycete Pathogen Phytophthora parasitica
Meixiang Zhang, Qinhu Wang, Ke Xu, Yuling Meng, Junli Quan, Weixing Shan
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0028114
Abstract: Species of the oomycete genus Phytophthora are destructive pathogens, causing extensive losses in agricultural crops and natural ecosystems. A potential disease control approach is the application of RNA silencing technology which has proven to be effective in improving plant resistance against a wide range of pests including parasitic plants, nematodes, insects and fungi. In this study, we tested the potential application of RNA silencing in improving plant disease resistance against oomycete pathogens. The endogenous P. parasitica gene PnPMA1 and the reporter gene GFP were used to evaluate the potential application of host induced gene silencing (HIGS). The GFP-expressing P. parasitica efficiently colonized Arabidopsis thaliana lines stably expressing GFP dsRNA and showed no obvious decrease in GFP signal intensity. Quantitative RT-PCR analyses showed no significant reductions in the abundance of GFP and PnPMA1 transcripts in P. parasitica during colonization of A. thaliana lines stably expressing GFP and PnPMA1 dsRNAs, respectively. Neither GFP siRNAs nor PnPMA1 siRNAs produced by transgenic plants were detected in P. parasitica re-isolated from infected tissues by Northern blot analyses. Phenotypic characterization of zoospores released from infected plant roots expressing PnPMA1 dsRNA showed no motility changes compared with those from wild-type plants. Similar results were obtained by analysis of zoospores released from sporulating hyphae of P. parasitica re-isolated from PnPMA1 dsRNA-expressing plant roots. Thus, the ectopic expression of dsRNA sequences in the host plant is not sufficient to initiate silencing of homologous genes in the colonizing oomycete pathogen, and this may be due to a number of different reasons including the absence of genetic machinery required for uptake of silencing signals in particular dsRNAs which are essential for environmental RNA silencing.
Computational prediction and experimental verification of miRNAs in Panicum miliaceum L.
YongJun Wu,JiangFeng Du,XiaoLong Wang,XiaoFeng Fang,WeiXing Shan,ZongSuo Liang
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4367-y
Abstract: MicroRNAs (miRNAs) are a class of non-coding RNAs that play critical roles in post-transcriptional regulation. Their target genes are involved in a variety of biological processes such as development, metabolism, and stress response. Panicum miliaceum L. (Panicum) is an important grain crop, but, until now, no miRNAs have been identified in this plant. Using a homology search based on expressed sequence tag (EST) analysis and miRNA precursor secondary structure, a total of 43 new miRNAs were identified. The miRNAs were found to be unevenly distributed among 11 miRNA families. Target analysis using the plant small RNA target analysis server psRNATarget showed that the newly identified miRNAs can potentially regulate 68 target genes. Ten of the 11 miRNA families were annotated as involved in RNA regulation, suggesting they may play an essential role in post-transcriptional regulation in Panicum. Selected miRNAs representing eight of the families were verified by northern blotting, indicating that the prediction method that we used to identify the miRNAs was effective.
-clean rings
Weixing Chen
International Journal of Mathematics and Mathematical Sciences , 2006, DOI: 10.1155/ijmms/2006/75247
Abstract: In this paper we unify the structures of various clean rings by introducing the notion of P-clean rings. Some properties of P-clean rings are investigated, which generalize the known results on clean rings, semiclean rings, n-clean rings, and so forth. By the way, we answer a question of Xiao and Tong on n-clean rings in the negative.
On Semiabelian -Regular Rings
Weixing Chen
International Journal of Mathematics and Mathematical Sciences , 2007, DOI: 10.1155/2007/63171
Abstract: A ring R is defined to be semiabelian if every idempotent of R is either right semicentral or left semicentral. It is proved that the set N(R) of nilpotent elements in a π-regular ring R is an ideal of R if and only if R/J(R) is abelian, where J(R) is the Jacobson radical of R. It follows that a semiabelian ring R is π-regular if and only if N(R) is an ideal of R and R/N(R) is regular, which extends the fundamental result of Badawi (1997). Moreover, several related results and examples are given.
The Establishment of the Internal Control Frame System of Colleges
Weixing Wang
International Journal of Business and Management , 2010, DOI: 10.5539/ijbm.v5n5p119
Abstract: The key to establish the internal control frame system of colleges is to confirm the internal control target and the control factors. The internal control target concludes the schooling effect and efficiency, the safety and integrity of assets, the real, complete, and useful accounting and management information, and following national laws and regulations. The factors of internal control could be divided into two layers. The first layer is composed by the control environment, the control system, and the supervision and evaluation, and the control environment is the base, the control system is the core and the supervision and evaluation are the guarantee.
空间科学学报 , 2008,
Abstract: ThispaperreviewsvariousprogressesontheionosphericstudiesbythescientistsinChinaduringthelasttwoyears.Themaincontentsconcernthe4aspectsoftheionosphericresearch:(1)ionosphericweatherandcouplingwithmagnetosphere(polarandauroralionosphere,ionosphericresponsetosubstorms,ionosphericstorms);(2)mid-andlow-latitudeionosphericclimatology(ionosphericproperties,yearlyvariationsandsolaractivitydependence,longtermvariation);(3)ionosphericcouplingwithneutralatmosphere(gravitywaves,tides,planetarywaves,backgroundupperatmosphere,andionosphericresponse);and(4)ionosphericdiagnostics(observation,modeling,andprediction).
空间科学学报 , 2010,
Abstract: Intheionosphericresearch,variousprogresseshavebeenmadeduringthelasttwoyears.ThispaperreviewstherecentworksofChinesescientists.Forconvenience,thecontentsinclude:ionosphericstormsandspaceweather;ionosphericirregularitiesandscintillation;ionosphericvariability;ionosphericdisturbances;ionosphericresponsetosolareclipses;ionosphericcouplingwithatmosphereandlithosphere;ionosphericclimatology;ionosphericmodeling;andionosphericpredictionandapplication.
Prediction of Cosmological Constant Λ in Veneziano Ghost Theory of QCD  [PDF]
Lijuan Zhou, Weixing Ma, Leonard S. Kisslinger
Journal of Modern Physics (JMP) , 2012, DOI: 10.4236/jmp.2012.329151
Abstract: Based on the Veneziano ghost theory of QCD, we estimate the cosmological constant Λ, which is related to the vacuum energy density, , by =8πG . In the recent Veneziano ghost theory is given by the absolute value of the product of the local quark condensate and quark current mass: =(2NfH/m)*c|mq<0|:qq:|0>|.By solving Dyson-Schwinger Equations for a dressed quark propagator, we found the local quark condensate <0|:qq:|0>; -(235 MeV)3, the generally accepted value. The quark current mass is mq 4.0 Mev. This gives the same result for as found by previous authors, which is somewhat larger than the observed value. However, when
Rust Secreted Protein Ps87 Is Conserved in Diverse Fungal Pathogens and Contains a RXLR-like Motif Sufficient for Translocation into Plant Cells
Biao Gu, Shiv D. Kale, Qinhu Wang, Dinghe Wang, Qiaona Pan, Hua Cao, Yuling Meng, Zhensheng Kang, Brett M. Tyler, Weixing Shan
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0027217
Abstract: Background Effector proteins of biotrophic plant pathogenic fungi and oomycetes are delivered into host cells and play important roles in both disease development and disease resistance response. How obligate fungal pathogen effectors enter host cells is poorly understood. The Ps87 gene of Puccinia striiformis encodes a protein that is conserved in diverse fungal pathogens. Ps87 homologs from a clade containing rust fungi are predicted to be secreted. The aim of this study is to test whether Ps87 may act as an effector during Puccinia striiformis infection. Methodology/Principal Findings Yeast signal sequence trap assay showed that the rust protein Ps87 could be secreted from yeast cells, but a homolog from Magnaporthe oryzae that was not predicted to be secreted, could not. Cell re-entry and protein uptake assays showed that a region of Ps87 containing a conserved RXLR-like motif [K/R]RLTG was confirmed to be capable of delivering oomycete effector Avr1b into soybean leaf cells and carrying GFP into soybean root cells. Mutations in the Ps87 motif (KRLTG) abolished the protein translocation ability. Conclusions/Significance The results suggest that Ps87 and its secreted homologs could utilize similar protein translocation machinery as those of oomycete and other fungal pathogens. Ps87 did not show direct suppression activity on plant defense responses. These results suggest Ps87 may represent an “emerging effector” that has recently acquired the ability to enter plant cells but has not yet acquired the ability to alter host physiology.
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