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Search Results: 1 - 10 of 80790 matches for " WanHong Liu "
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Exploration and Research on HIV/AIDS Patients’ Complement C1q Level Changes before and after Treatments  [PDF]
Guosheng Su, Xiaolu Luo, Shunda Luo, Zeduan Liu, Zuyan Ni, Wanhong Huang, Juanying Liang
Advances in Infectious Diseases (AID) , 2015, DOI: 10.4236/aid.2015.53013
Abstract: Objective: The study aimed to investigate the complement C1q test results of HIV/AIDS patients in clinical application before and after treatments. Methods: We collected HIV/AIDS patients’ serum specimens storing at -80 centigrade freezer in cryogenic refrigerator for standby. After samples quantity met the requirements of selected cases unified, complement C1q was detected by immune transmission turbidity method, and compared the differences in complement C1q of HIV/AIDS patients in test results before and after treatment. In the collection of 96 cases selected samples, concentration of complement C1q was 157.95 ± 31.46 mg/L before treatment, while after treatment, it was 147.26 ± 28.76 mg/L. Comparing the results before and after treatment,t= 2.45726,P= 0.01049, the difference was statistically significant. Concentration of complement C1q increased after treatment with 33 cases. There were 63 cases reducing. Through statistical analysis on the data from the number of reducing and increasing cases, chi-square = 18.75,P= 0.00356, the difference was statistically significant. Complement C1q detection in the treatment of patients with HIV/AIDS had an important clinical significance in the process. The analysis of the concentration changes before and after treatment was clinically significant for drug selection and monitoring disease progression and curative effects, which would be worth further researching.
Development of Viral Vectors for Gene Therapy for Chronic Pain
Yu Huang,Xin Liu,Lanlan Dong,Zhongchun Liu,Xiaohua He,Wanhong Liu
Pain Research and Treatment , 2011, DOI: 10.1155/2011/968218
Abstract: Chronic pain is a major health concern that affects millions of people. There are no adequate long-term therapies for chronic pain sufferers, leading to significant cost for both society and the individual. The most commonly used therapy for chronic pain is the application of opioid analgesics and nonsteroidal anti-inflammatory drugs, but these drugs can lead to addiction and may cause side effects. Further studies of the mechanisms of chronic pain have opened the way for development of new treatment strategies, one of which is gene therapy. The key to gene therapy is selecting safe and highly efficient gene delivery systems that can deliver therapeutic genes to overexpress or suppress relevant targets in specific cell types. Here we review several promising viral vectors that could be applied in gene transfer for the treatment of chronic pain and further discuss the possible mechanisms of genes of interest that could be delivered with viral vectors for the treatment of chronic pain. 1. Introduction Chronic pain is defined by the U.S. Food and Drug Administration as pain that persists for more than 3 months [1]. This leads to great suffering for patients and results in a heavy burden for society. Chronic pain is not simply related to anatomical reorganization; other changes in neurotransmission and electrophysiological activity are also involved in the pain pathway [2]. Conventional drug treatment has many limitations, such as drug dependence, tolerance, respiratory depression, and other systemic side effects. The development of gene therapy has opened the possibility of using either nonviral or viral vectors to transduce genes encoding antinociceptive substances to treat chronic pain. Compared with nonviral systems, viral vectors are much more efficient in delivering exogenous genes to target cells and inducing long-term gene expression [3]. However, not all viruses are suitable for gene delivery. For instance, murine leukemia virus and lentivirus are both retroviruses, but lentivirus can infect nondividing cells, while murine leukemia virus cannot. This is the reason murine leukemia virus is usually not used as a gene carrier in neurological disease treatments [4]. It was reported that 2 of 11 children receiving gene therapy using a retroviral vector caused leukemia. A possible reason is the integration of viral gene may activate oncogenes. So the safety of retroviral vectors has been paid more and more attention [5, 6]. The ideal gene therapy vector should not be able to replicate its own DNA and be conducive to long-term gene expression. In
Depth zonation of Arenigian acritarchs in South China
Wanhong Xu
Chinese Science Bulletin , 1997, DOI: 10.1007/BF02882448
Interaction between CRHR1 and BDNF Genes Increases the Risk of Recurrent Major Depressive Disorder in Chinese Population
Zheman Xiao, Wanhong Liu, Kai Gao, Qirong Wan, Can Yang, Huiling Wang, Xiaoping Wang, Gaohua Wang, Zhongchun Liu
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0028733
Abstract: Background An important etiological hypothesis about depression is stress has neurotoxic effects that damage the hippocampal cells. Corticotropin-releasing hormone (CRH) regulates brain-derived neurotrophic factor (BDNF) expression through influencing cAMP and Ca2+ signaling pathways during the course. The aim of this study is to examine the single and combined effects of CRH receptor 1 (CRHR1) and BDNF genes in recurrent major depressive disorder (MDD). Methodology/Principal Finding The sample consists of 181 patients with recurrent MDD and 186 healthy controls. Whether genetic variations interaction between CRHR1 and BDNF genes might be associated with increased susceptibility to recurrent MDD was studied by using a gene-based association analysis of single-nucleotide polymorphisms (SNPs). CRHR1 gene (rs1876828, rs242939 and rs242941) and BDNF gene (rs6265) were identified in the samples of patients diagnosed with recurrent MDD and matched controls. Allelic association between CRHR1 rs242939 and recurrent MDD was found in our sample (allelic: p = 0.018, genotypic: p = 0.022) with an Odds Ratio 0.454 (95% CI 0.266–0.775). A global test of these four haplotypes showed a significant difference between recurrent MDD group and control group (chi-2 = 13.117, df = 3, P = 0.016. Furthermore, BDNF and CRHR1 interactions were found in the significant 2-locus, gene–gene interaction models (p = 0.05) using a generalized multifactor dimensionality reduction (GMDR) method. Conclusion Our results suggest that an interaction between CRHR1 and BDNF genes constitutes susceptibility to recurrent MDD.
Preparation and evaluation of a new releasable PEGylated tumor necrosis factor-α (TNF-α) conjugate for therapeutic application
ChuanYun Dai,Ya Fu,ShaoCheng Chen,Biao Li,Bo Yao,WanHong Liu,LiQing Zhu,Nan Chen,Ji Chen,Qiang Zhang
Science China Life Sciences , 2013, DOI: 10.1007/s11427-012-4431-7
Abstract: To design a releasable PEGylated TNF-α (rPEG-TNF-α), a cathepsin B-sensitive dipeptide (Val-Cit moiety) was inserted into conventional PEG-modified TNF-α (PEG-TNF-α), facilitating its clinical use for anti-tumor therapy. Comparative pharmacokinetic and pharmacodynamic studies showed that the half-lives of both PEGylated forms of TNF-α were ~60-fold greater than that of unmodified TNF-α. In addition, the in vitro bioactivity of rPEG-TNF-α was greater than that of PEG-TNF-α with the same degree of PEG modification. Release of TNF-α from rPEG-TNF-α in vitro was dependent on the presence of cathepsin B and was inhibited by a cathepsin B inhibitor. Despite the potent cytotoxicity of unmodified TNF-α against normal cells, its PEGylated forms at higher TNF-α concentrations showed low cytotoxic activity against these cells. In contrast, both forms of PEGylated TNF-α showed potent cytotoxic activity against the B16 and L929 cell lines, with rPEG-TNF-α being 5- and 9-fold more potent, respectively, than PEG-TNF-α. Moreover, rPEG-TNF-α was a more potent in vivo antitumor agent than PEG-TNF-α.
Transcriptome Analysis of the Hippocampus in Novel Rat Model of Febrile Seizures
Zhongcheng Wang, Yuanteng Fan, Jian Xu, Liang Li, Duanhe Heng, Song Han, Jun Yin, Biwen Peng, Wanhong Liu, Xiaohua He
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095237
Abstract: Febrile seizures (FS) are the most common type of convulsive events in infants and young children, but the precise underlying genetic mechanism remains to be explored. To investigate the underlying pathogenic factors in FS and subsequent epilepsy, alterations in gene expression between the two new strains of rats (hyperthermia-prone [HP] vs hyperthermia-resistant [HR]), were investigated by using the Whole Rat Genome Oligo Microarray. This process identified 1,140 differentially expressed genes (DEGs; 602 upregulated and 538 downregulated), which were analyzed to determine significant Gene Ontology (GO) categories, signaling pathways and gene networks. Based on the GO analyses, the modified genes are closely related to various FS pathogenesis factors, including immune and inflammatory responses and ion transport. Certain DEGs identified have not been previously examined in relation to FS pathogenesis. Among these genes is dipeptidyl peptidase 4 (DPP4), a gene closely linked to interleukin 6 (IL-6), which played a key role in the gene network analysis. Furthermore, sitagliptin, a DPP4 inhibitor significantly decreased epileptic discharge in rats, observed via electroencephalogram, suggesting an important role for DPP4 in FS. The effectiveness of sitagliptin in reducing seizure activity may occur through a mechanism that stabilizes cellular Ca2+ homeostasis. In addition, DPP4 expression may be regulated by DNA methylation. The hippocampal gene expression profiles in novel rat models of FS provides a large database of candidate genes and pathways, which will be useful for researchers interested in disorders of neuronal excitability.
Depth zonation of Arenigian acritarchs in South China

Wanhong Xu,

科学通报(英文版) , 1997,
A Reference Proteomic Database of Lactobacillus plantarum CMCC-P0002
Li Zhu, Wei Hu, Datao Liu, Wanhong Tian, Gang Yu, Xiankai Liu, Jie Wang, Erling Feng, Xuemin Zhang, Bei Chen, Ming Zeng, Hengliang Wang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0025596
Abstract: Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.
The habitat characteristics of Eurasian badger in Beijing-Hangzhou Grand Canal embankment

YIN Baof,LIU Yuqing,LIU Guoxing,WEI Wanhong,

生态学报 , 2011,
Abstract: Habitat selection is one of the most poorly understood ecological processes, but its study has become a necessary tool in conservation biology and wildlife management. The basic assumption in habitat selection theory is that individuals select those types of habitat where the benefits are maximized and the harm is minimized. Many variables can influence the individual's decision of selecting between available habitats, from physiological limitations to ecological constrains (e.g. predation pressure, refuge). The knowledge of these factors is basic for the correct understanding of habitat choice processes and for the implementation of effective strategies in conservation and/or management of species and landscapes. The Eurasian badger (Meles meles) is a medium sized carnivore distributed all over temperate Eurasia. In October-November 2006 and 2007, the habitat selection of Eurasian badger was investigated at Beijing-Hangzhou Grand Canal embankment (from Shaobo to Gaoyou). Two indexes of activity intensities, setts number and feces number were used to estimate the utilization intensities of badger on three types of habitats,Populus euphratica artificial forest,Populus euphratica-Paulownia fortunei artificial forest and Populus euphratica artificial forest. Meanwhile, the factors influencing the habitat selection of badger were analyzed by stepwise regression for determining the habitat characteristics of Eurasian badger. Results indicated that, in Beijing-Hangzhou Grand Canal embankment area, badgers preferred Populus euphratica forest and Populus euphratica-Paulownia fortunei artificial forest attribute to dense plant canopy. Stepwise regression indicated that density of shrubs, density of big trees, herbaceous plants cover, soil moisture and human disturbance degree significantly influenced the number of setts (P=0.002). Furthermore, the number of fences were also affected by the density of shrubs and big trees, and human disturbance(P=0.012). Overall, the influences of most habitat variables on the habitat selection of badger were directly or indirectly related to shelter cover and predation risk. It is concluded that badgers preferred to selecting forests with dense plant canopy and lower predation risk.
Engineering Salidroside Biosynthetic Pathway in Hairy Root Cultures of Rhodiola crenulata Based on Metabolic Characterization of Tyrosine Decarboxylase
Xiaozhong Lan, Kai Chang, Lingjiang Zeng, Xiaoqiang Liu, Fei Qiu, Weilie Zheng, Hong Quan, Zhihua Liao, Min Chen, Wenlin Huang, Wanhong Liu, Qiang Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075459
Abstract: Tyrosine decarboxylase initializes salidroside biosynthesis. Metabolic characterization of tyrosine decarboxylase gene from Rhodiola crenulata (RcTYDC) revealed that it played an important role in salidroside biosynthesis. Recombinant 53 kDa RcTYDC converted tyrosine into tyramine. RcTYDC gene expression was induced coordinately with the expression of RcUDPGT (the last gene involved in salidroside biosynthesis) in SA/MeJA treatment; the expression of RcTYDC and RcUDPGT was dramatically upregulated by SA, respectively 49 folds and 36 folds compared with control. MeJA also significantly increased the expression of RcTYDC and RcUDPGT in hairy root cultures. The tissue profile of RcTYDC and RcUDPGT was highly similar: highest expression levels found in stems, higher expression levels in leaves than in flowers and roots. The gene expressing levels were consistent with the salidroside accumulation levels. This strongly suggested that RcTYDC played an important role in salidroside biosynthesis in R. crenulata. Finally, RcTYDC was used to engineering salidroside biosynthetic pathway in R. crenulata hairy roots via metabolic engineering strategy of overexpression. All the transgenic lines showed much higher expression levels of RcTYDC than non-transgenic one. The transgenic lines produced tyramine, tyrosol and salidroside at higher levels, which were respectively 3.21–6.84, 1.50–2.19 and 1.27–3.47 folds compared with the corresponding compound in non-transgenic lines. In conclusion, RcTYDC overexpression promoted tyramine biosynthesis that facilitated more metabolic flux flowing toward the downstream pathway and as a result, the intermediate tyrosol was accumulated more that led to the increased production of the end-product salidroside.
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