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Search Results: 1 - 10 of 15648 matches for " Wan-Soon Park "
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Intracellular ATP Assay of Live Cells Using PTD-Conjugated Luciferase
Mi-Sook Lee,Wan-Soon Park,Young Han Kim,Won Gyeong Ahn,Seung-Hae Kwon,Song Her
Sensors , 2012, DOI: 10.3390/s121115628
Abstract: Luciferase is a sensitive, reliable biological sensor used for measuring ATP. However, its widespread application in drug discovery and toxicology studies has been limited due to unavoidable cell extraction processes, which cause inaccurate measurements of intracellular ATP and obstruct the application of homogenous high-throughput screening. Recently, we developed a protein transduction domain-conjugated luciferase (PTD-Luc) for measuring cellular uptake efficacy. In this study, we evaluated the applicability of PTD-Luc to an intracellular ATP assay of live cells. The predominant fluorescence of Alexa 647-PTD-Luc was in the cytosol, whereas the fluorescence of Alexa 647-Luc was visualized surrounding the cell membrane, as confirmed by Western blot analysis. In vitro, PTD-Luc could detect less than 10–9 M ATP, and the correlation between the luciferase activity of PTD-Luc and the ATP content was strong (R = 0.999, p < 0.001). In vivo, luminescence signals of PTD-Luc detected intracellular ATP in as few as 50 HeLa cells, with a strong correlation between luminescence and cell number, suggesting high sensitivity and reliability. Furthermore, two blockers of the glycolytic pathway (2-deoxyglucose and iodoacetic acid) inhibited the signal in a dose-dependent manner, whereas potassium cyanide, an inhibitor of oxidative phosphorylation, had no effect on intracellular ATP in vivo, as seen with the PTD-Luc sensor. These data show that PTD-Luc can directly measure the intracellular ATP content in live cells, allowing real-time kinetic studies, suggesting that it is a promising tool for high-throughput drug screening and cytotoxicity assays.
Willingness to Pay for the Subscription Fee of Public Broadcasting System  [PDF]
Inho Park, Hyun Soon Park
Advances in Journalism and Communication (AJC) , 2014, DOI: 10.4236/ajc.2014.21001
Abstract: The study aims at developing communication strategies for the public broadcasting system according to WTP (Willingness to Pay) for the subscription fee and the audience’s perception of the public broadcasting system as a public good. A quasi-experiment was conducted for audiences who have previously watched public broadcasting channels. According to the results, those respondents who think of public broadcasting as a public good and who think that public broadcasting performs its public role well have high intention to pay the subscription fee. Respondents feel at a loss when they pay the subscription fee, and the willingness to pay the fee goes down.
Global embeddings of scalar-tensor theories in (2+1)-dimensions
Soon-Tae Hong,Won Tae Kim,Yong-Wan Kim,Young-Jai Park
Physics , 2000, DOI: 10.1103/PhysRevD.62.064021
Abstract: We obtain (3+3)- or (3+2)-dimensional global flat embeddings of four uncharged and charged scalar-tensor theories with the parameters B or L in the (2+1)-dimensions, which are the non-trivially modified versions of the Banados-Teitelboim-Zanelli (BTZ) black holes. The limiting cases B=0 or L=0 exactly are reduced to the Global Embedding Minkowski Space (GEMS) solution of the BTZ black holes.
Higher dimensional flat embeddings of (2+1) dimensional black holes
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park
Physics , 2000, DOI: 10.1103/PhysRevD.62.024024
Abstract: We obtain the higher dimensional global flat embeddings of static, rotating, and charged BTZ black holes. On the other hand, we also study the similar higher dimensional flat embeddings of the (2+1) de Sitter black holes which are the counterparts of the anti-de Sitter BTZ black holes. As a result, the charged dS black hole is shown to be embedded in (3+2) GEMS, contrast to the charged BTZ one having (3+3) GEMS structure.
Symplectic quantization of self-dual master Lagrangian
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park,Klaus D. Rothe
Physics , 2002, DOI: 10.1088/0305-4470/35/34/315
Abstract: We consider the master Lagrangian of Deser and Jackiw, interpolating between the self-dual and the Maxwell-Chern-Simons Lagrangian, and quantize it following the symplectic approach, as well as the traditional Dirac scheme. We demonstrate the equivalence of these procedures in the subspace of the second-class constraints. We then proceed to embed this mixed first- and second-class system into an extended first-class system within the framework of both approaches, and construct the corresponding generator for this extended gauge symmetry in both formulations.
Consistent Dirac Quantization of SU(2) Skyrmion equivalent to the BFT Scheme
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park
Physics , 1998, DOI: 10.1103/PhysRevD.59.114026
Abstract: In the framework of Dirac quantization, the SU(2) Skyrmion is canonically quantized to yield the modified predictions of the static properties of baryons. We show that the energy spectrum of this Skyrmion obtained by the Dirac method with the suggestion of generalized momenta is consistent with the result of the Batalin-Fradkin-Tyutin formalism.
Symplectic embedding and Hamilton-Jacobi analysis of Proca model
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park,K. D. Rothe
Physics , 2001, DOI: 10.1142/S0217732302006746
Abstract: Following the symplectic approach we show how to embed the Abelian Proca model into a first-class system by extending the configuration space to include an additional pair of scalar fields, and compare it with the improved Dirac scheme. We obtain in this way the desired Wess-Zumino and gauge fixing terms of BRST invariant Lagrangian. Furthermore, the integrability properties of the second-class system described by the Abelian Proca model are investigated using the Hamilton-Jacobi formalism, where we construct the closed Lie algebra by introducing operators associated with the generalized Poisson brackets.
Symmetries of SU(2) Skyrmion in Hamiltonian and Lagrangian approaches
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park
Physics , 1999, DOI: 10.1142/S0217732300000086
Abstract: We apply the Batalin-Fradkin-Tyutin (BFT) method to the SU(2) Skyrmion to study the full symmetry structure of the model at the first class Hamiltonian level. On the other hand, we also analyze the symmetry structure of the action having the WZ term, which corresponds to this Hamiltonian, in the framework of the Lagrangian approach. Furthermore, following the BFV formalism we derive the BRST invariant gauge fixed Lagrangian from the above extended action.
Improved Hamilton-Jacobi Quantization for Nonholonomic Constrained System
Soon-Tae Hong,Won Tae Kim,Yong-Wan Kim,Young-Jai Park
Physics , 2001,
Abstract: The nonholonomic constrained system with second-class constraints is investigated using the Hamilton-Jacobi (HJ) quantization scheme to yield the complete equations of motion of the system. Although the integrability conditions in the HJ scheme are equivalent to the involutive relations for the first-class constrained system in the improved Dirac quantization method (DQM), one should elaborate the HJ scheme by using the improved DQM in order to obtain the first-class Hamiltonian and the corresponding effective Lagrangian having the BRST invariant nonholonomic constrained system.
Constraint structure of O(3) nonlinear sigma model revisited
Soon-Tae Hong,Yong-Wan Kim,Young-Jai Park,Klaus D. Rothe
Physics , 2002, DOI: 10.1088/0305-4470/36/6/309
Abstract: We study the constraint structure of the O(3) nonlinear sigma model in the framework of the Lagrangian, symplectic, Hamilton-Jacobi as well as the Batalin-Fradkin-Tyutin embedding procedure.
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