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Search Results: 1 - 10 of 150 matches for " WIDYA EKA PRAYITNO "
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Stability of Viable Counts of Lactic Acid Bacteria during Storage of Goat Milk Soft Cheese
WINIATI PUDJI RAHAYU,FERI KUSNANDAR,WIDYA EKA PRAYITNO
Microbiology Indonesia , 2011, DOI: 10.5454/mi.5.4.1
Abstract: The use of goat milk is limited in Indonesia due to lack of good milking practices resulted in disliked goaty smell. One of the method to eliminate this off flavor is by processing the goat milk into soft cheese. The aim of this research was to study the stability of viable starter lactic acid bacteria cultures (Lactobacillus acidophilus FNCC-0051 and L. casei FNCC-0090) during storage of goat milk soft cheese. Three batches of goat milk soft cheeses were produced with different starter cultures L. acidophilus FNCC-0051 (5.0 x 106 cfu mL-1); L. casei FNCC-0090 (5.0 x 106 cfu mL-1); and the mixture of L. acidophilus FNCC-0051 (2.5 x 106 cfu mL-1) and L. casei FNCC-0090 (2.5 x 106 cfu mL-1). The goat milk cheeses had white color and soft. The viable lactic acid bacteria in the goat milk soft cheese reached 109 cfu g-1, which was stable for 8 weeks at 5 °C. Panelists liked goat milk soft cheeses, especially in term of its aroma. The specific aroma produced could mask the disliked goaty smell.
Extraction of Collagen from Chicken Feet with Various Acidic Solutions and Soaking Time
Prayitno
Journal of Animal Production , 2007,
Abstract: The objective of this research was to know the ability of various acidic solutions on dissolving collagen chicken feet, with different soaked time. Each acid 5 percent (v/v), collagen extraction was done by washing chicken feet and then cutted into small pieces and finally grinded. Every 100 gram treatment was soaked in acetic acid (a1), citric acid (a2), lactic acid (a3) and hydrochloric acid (a4), for 12, 24 and 36 hours. Precipitated collagen in the filtrate was 5 percent NaOH to reach the neutral pH (pH 7). Collagen precipitate was separated by filtration usingfilter paper and then rendement was calculated, HPLC was used to determin amino acid composition, and SDS-PAGE was use determin the type of collagen. This experiment use factorial completely randomized design (CRD) 4 x 3 and three time replication. Result showed that lactic acid has highest capability to dissolve collagen, while citric acid the lowest. Combination of acid solution and soaking time had significant (P<0.01) effect on dissolving collagen of chicken feet. Extracted collagen in all acid solution, hassame amino acid, composition but different in percentage of amino acid molecules. Collagen type in treatment combination was the same, but for soaking time of 36 hours revealed some peptide band. Lactic acid had highest capability of collagen extraction in chicken feet than citric acid, acetic acid and hydrochloric acid with soaking time of 12, 24 and 36 hours. It was estimated that extracted collagen can be grouped to type I consisted of two chain of a1. (Animal Production 9(2): 99-104 (2007)Key Words : Chicken feet, acids, soaking time, collagen
The Content of Lactic Acid and Lactose of Yoghurt Fermented with Different Number and Percentage Starter Bacteria
Prayitno
Journal of Animal Production , 2006,
Abstract: The objective of the research was to study rate of lactose fermentation and production of lactic acid by Streptococus thermophillus and Lactobacillus bulgaricus. Yoghurt was made from skim milk 10 percent inoculated with Streptococus thermophillus and Lactobacillus bulgaricus different number and percentage. The research was conducted in a completely randomzed design factorial pattern 3 x 4. The first factor was different (R) of Strepcocus thermophillus and Lactobacillus bulgaricus 109 : 109 (1 : 1), 109 : 2x109 (1 : 2) and 2x109 : 109 (2 : 1), and second factor was percentage (S) of starter, with were 1, 2, 3 and 4 percent. Each treatment has three replicated. Variabels measured ware lactic acid and lactose content of yoghurt after 12 hours fermented. Lactic acid content was determined by titration and lactose content by spectrofotometry. Result showed that number of Streptococus thermophillus and Lactobacillus bulgaricus and percentage of starter, including ther interaction, have very hight significant (P<0,01) affect on lactic acid and lactose content of yoghurt. For resulted the yoghurt with the best quality (midle of lactic acid and lower lactose content) recommended to used Streptococus thermophillus and Lactobacillus bulgaricus 1 : 2 with 4 percent starter addition. (Animal Production 8(2): 131-136 (2006) Key Words : Streptococus thermophillus, Lactobacillus bulgaricus, starter, yoghurt, lactic acid, lactose
Performance of Cement for Immobilizing Strontium Waste in Saline Environment  [PDF]
Susetyo Hario Putero, Widya Rosita
Materials Sciences and Applications (MSA) , 2013, DOI: 10.4236/msa.2013.412A002
Abstract:

For solving the radioactive waste storage problem, there is an idea to store immobilized waste at deep sea. Solidifier material, such as cement should be resistance to saline environment for deep sea storage. So, this research objective is to study the performance of cementation method in immobilizing strontium waste in saline environment. Research was conducted by immobilizing strontium waste using Portland pozzolanic cement, white cement and composite Portland cement. Cement, 65 ppm Sr(NO3)2, sand and water were mixed and cast. Strontium waste varied in 2 v/o, 4 v/o, 6 v/o and 8 v/o. After 28 days curing, the cement block’s compressive strength and leaching rate on saline water were analyzed. Determination of compressive strength was

Growth, cellulose, and lignin content of ramie (Boehmeria nivea L. Gaudich) with treatment of Gibberelic Acid (GA3 )
WIDYA MUDYANTINI
Biodiversitas , 2008,
Abstract: The aims of this research were to: (i) study the effects of gibberellic acid (GA3) to the growth of Boehmeria nivea L. Gaudich, and (ii) study the effects of gibberellic acid (GA3) to the cellulose and lignin content of B. nivea. GA3 could delivered starch hydrolysis that could support α-amylase formation that would increase glucose. Increasing glucose concentration caused the rising of osmotic pressure inside the cell so that the cells expand. The initial synthetic path of cellulose was glucose, as well as lignin. If GA3 could increased the glucose amount in plant, then the cellulose and lignin amount also increased. This experiment use Completely Random Design with one factor, they are concentration gibberellic acid that consist of 6 treatment concentration are GA3 0 ppm, GA3 50 ppm, GA3 100 ppm, GA3 150 ppm, GA3 200 ppm, and GA3 250 ppm. The treatment to the rhizome had been done before it was planted. Some parameters like growth and anatomy parameter are measured. The results showed that treatment GA3 affect to the improvement of bud stem diameter, length of bud stem, the wet weight, dry weight, length of phloem bundle, phloem number, and lignin content but they does not affect to the change of bud number, leaf number, and cellulose content. The treatment of GA3 on GA3 200 ppm show the maximum result to the improvement of bud stem diameter, length of bud stem, the wet weight, dry weight, length of phloem bundle, phloem number, and cellulose content The treatment of GA3 on GA3 250 ppm show maximum result to the improvement of bud number, leaf number, and lignin content.
Root and Nodulation Phenotypes of the Ethylene-Insensitive Sickle Mutant of Medicago truncatula
JOKO PRAYITNO
HAYATI Journal of Biosciences , 2010,
Abstract: The sickle (skl) mutant of the model legume Medicago truncatula is an ethylene-sensitive mutant that have a ten-fold increase in nodule numbers. The nodulation and root phenotypes of the skl mutant were investigated and further characterised. The skl mutant had longer roots than the wild type, but when inoculated with Sinorhizobium, its root length was reduced to the level of wild type. Furthermore, lateral root numbers in uninoculated skl were similar to those in uninoculated wild type. However, when the root tips were decapitated, fewer lateral roots formed in skl than in wild type. Nodule numbers of the skl mutant were significantly reduced by low nitrate concentration (2.5 mM). These results suggest that skl mutant has alterations in both root and nodule development.
Cervical cancer with Human Papilloma Virus and Epstein Barr Virus positive
Prayitno Adi
Journal of Carcinogenesis , 2006, DOI: 10.1186/1477-3163-5-13
Abstract: The Early-7 (E7) protein of HPV binds to the underphosphorelated form of the tumor suppressor protein – pRb and displaces the E2F transcription factor that is normally bound by pRb. The latent membrane protein-1 (LMP-1) of EBV prevents apoptosis of B cells by up regulating the expression of bcl-2, and it activates growth promoting pathway that are normally triggered by T cell – derivate signal. The aims of this study to know that in cervical cancer stay HPV and EBV. DNA was isolated from nineteen sample cervical cancer tissues frozen section. Diagnose related with HPV and EBV was made by Polymerase Chains Reaction (PCR). The result of this experiment showed that from 19 samples diagnosed as cervical cancer, 17 samples are positive HPV and 13 samples had HPV and EBV positive. The conclusion of this experiment is 89% of cervical cancers are infected with HPV and 68% also infected with HPV and EBV.
REPRESENTASI GAMBARAN ALAM PADA PERWUJUDAN ARSITEKTUR PADMASANA DI BALI
I Nyoman Widya Paramadhyaksa
Bumi Lestari , 2013,
Abstract: The padmasana shrine is one of the main holy structures of Balinese Hindu. This shrine hasvarious ornaments, decorative elements and other forms that have symbolic meanings. Thesymbolic contents in padmasana have close relation with mythology and the concepts ofHindu teaching. Beside that, the concepts of architectural representation of padmasana alsohave a correlation with the nature representation of the earth. This article discusses about therepresentation of padmasana shrine as the symbol of mountain and the representation ofnature on earth.
The Relationship between Mouth Squamous Cell Carcinoma (MSCC) with HPV Infection and the Presence of p53 & c-myc Mutation  [PDF]
Adi Prayitno, Elyana Asnar, Suhartono Taat Putra
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.45105
Abstract:


Introduction: Now the molecular epidemiology is a new experience. It’s was noted that ninety percent of mouth cancers are squamous cell carcinomas and recorded 0.96% year of all cancers in Indonesia. Human papilloma virus (HPV) was implicated in pathogenesis of cancer. As a remark, that mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the relationship between the mouth squamous cell carcinoma (MSCC) with HPV infection, the presence of p53, and c-myc genes mutation. Methods and Material: Tissue biopsy frozen sections from Benign Mouth Squamous Cell (BMSC) and MSCC patients were collected from Mouth and Dental Department of Muwardi District Hospital in Solo—Indonesia. To amplify L1-HPV gene for fixed the HPV etiology, amplified p53 and c-myc genes continued with SSCP analysis and followed with measurement using densitometer to see mutation existence. The collected data were analyzed with Chi Square Test. Results: None of the sample of patients with BMSC with positive HPV showed p53 gene mutation or c-myc gene. From eleven samples obtained from patients with MSCC who were positive HPV showed 18.2% had mutations in the p53 gene and 9.1% had mutations in c-myc gene. The chisquare test was shown to have significant differences between the MSCC with HPV infection and the presence of p53 and c-myc genes mutation. Conclusion: HPV is a risk ingredient for MSCC.


ISLAM, ADAT, AND THE STATE: Matrifocality in Aceh Revisited
Eka Srimulyani
Al-Jami'ah : Journal of Islamic Studies , 2010,
Abstract: Matrifocality has been a rooted tradition in the social history of the community in Aceh. The principles of matrifocality have also affected on how women are positioned in the community, and the socio-gender relation within the community. The fact that Aceh has strongly associated to the Islamic values that claimed to support the paternal traditions. Apparently, the Islamic values and the local matrifocality practices juxtaposed through the roles of adat, which considered as inseparable to Islamic law or teaching, or in local term known as zat ngeun sifeut. Another point in revisiting matrifocality in Aceh in Aceh is an examination of how gender state ideology, particularly during the New Order Regime disregarded some local gender practices across some ethnics in the archipelago. Meanwhile, the state also hegemonied and promoted particular gender state ideology such as state ibuism. Nonetheless, the modernity and social changes have also contributed to the shifting of some matrifocality practices in contemporary Acehnese society. However, since the matrifocality has a strong root in the social life of the community, the principles of the matrifocality still survived until currently, although it transformed into ‘new matrifocality’ practices. Keywords: matrifocality, Aceh, gender, shari‘a law
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