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Search Results: 1 - 10 of 1843 matches for " WIDIASTUTI KARIM "
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Growth and Extracellular Carbonic Anhydrase Activity of Zooxanthellae Symbiodinium sp. in Response of Zinc Enrichment
WIDIASTUTI KARIM,RICHARDUS KASWADJI,TRI PRARTONO,LILY MARIA GORETTI PANGGABEAN
HAYATI Journal of Biosciences , 2011,
Abstract: Coral reef communities contain a wide variety of mutualistic associations none more important than the relationship between corals and their symbiotic dinoflagellates of the genus Symbiodinium sp., commonly referred to as zooxanthellae. The function of Zinc (Zn) as cofactor of several enzyme systems such as extracellular carbonic anhydrase (extracellular CA) which catalyzes the interconversion of HCO3- and CO2. Concentrations of dissolved Zn in oligothropic waters are often very low therefore may limit the growth of zooxanthellae and their ability to fix CO2 from seawater via the carbonic anhydrase. The aim of this research is to investigate the effect of various concentrations of Zn on the growth and extracellular CA activity in zooxanthellae. Cell density was monitored daily by enumeration with hemocytometer-type chamber (0.1 mm). Extracellular CA was measured in homogenized intact whole cell by a pH drift assay. Results revealed that Zn status strongly influences the growth rate and extracelullar CA activity in zooxanthellae. The specific growth rate and cell density increased two-fold whilst extracelullar CA activity increased 10.5 times higher than that in control with increasing concentrations of Zn from 0 to 80 nM, but decreased when Zn was over 80 nM. Under a concentration of 80 nM was not Zn limited culture, consequently the growth rate of zooxanthellae not dependent on CO2 concentration yet offset by extracelullar CA activity.
Characteristics of Phosphate-Solubilizing Bacteria Isolated from Acid Soil of Cikopomayak, West Java, Indonesia
HAPPY WIDIASTUTI
Microbiology Indonesia , 2008,
Abstract: Phosphate-solubilizing bacteria were isolated from acid soil from Cikopomayak. Microbiological assay showed that the bacteria were Gram negative, rod-shaped, and lacked red pigment on Pikovskaya medium. Isolate A synthesized red pigment on nutrient agar medium, while isolate B formed slightly red pigment on nutrient agar medium as well as on Voges Proskauer medium. The ratio of clearing zone to colony for isolate A and B were approximately 2.1 and 1.9, respectively. Biochemical assays showed that both isolates A and B utilized glucose, lactose, sucrose, mannitol, glycerol, mannose, urea, indole, and produced catalase, but neither produced amylase and oxidase. The bacteria are identified as Serratia marcescens. The ability to solubilize phosphate decreased or was even lost when subcultured on nutrient agar medium. However, the ability to solubilize phosphate was recovered gradually with the addition of phosphate. The results presented here suggest that the ability to solubilize phosphate and to synthesize the red pigment may be inversely correlated.
Pollen viability and its effect on fruit set of oil palm (Elaeis guineensis Jacq.)
ALFIN WIDIASTUTI,ENDAH RETNO PALUPI
Biodiversitas , 2008,
Abstract: The research was aimed at studying (1) the decline of pollen viability during storage, and (2) the effect of pollen amount on fruit set of oil palm (Elaeis guineensis Jacq.). The experiment was conducted at PT. Dami Mas Sejahtera and PT. Sinar Mas Agro Resource and Technology (SMART) Tbk, Riau from February to August 2004. The first experiment was investigated up to six months storage period in the refrigerator, whereas in the second experiment a randomized complete block design with two factors was used: length of storage, i.e. 0, 1 and 2 months and amount of pollen, i.e. 0.022, 0.044, 0.066, 0.088, and 0.11 g mixed with powder to 10g to pollinate an inflorescence. The result showed that the viability of pollen started to decline three months after storage from about 92% to 83%, and declined to about 75% after six months of storage. Result of the second experiment showed that storage of pollen up to two months did not affect percentage of normal fruit, although the percentage of parthenocarpic fruits was decreased. This could be due to the high viability of pollen as the viability was remained high (about 90%) after being stored for two months in the refrigerator. Pollen with high viability could be used in a smaller amount to pollinate a female inflorescence without affecting fruit set of about 70-76%.SD037 had a higher reproductive success than SD038 and SD39.
Conceptual Analysis and Fieldwork in Macroeconometric Methodology: Modeling Unemployment, Inflation and Production  [PDF]
Karim Errouaki
Technology and Investment (TI) , 2014, DOI: 10.4236/ti.2014.53015
Abstract:

The recent failure of commonly accepted, inductive, econometric models to provide insights into real, macroeconomic phenomenon during economic crises has provoked a debate concerning contemporary econometric methodology. Based on the foundations laid by Haavelmo, and Hollis and Nell, an assessment of Edward J. Nell’s (1998) “unifying methodological framework” (UMF) is offered. Nell’s UMF places socioeconomic institutions and interdependencies, and technological realities as basis of analysis. Using “conceptual analysis” and “fieldwork” Nell presents an alternative to generally accepted, mainstream, econometric methodology. The purpose of this paper is to look at some examples of the way, and this can help develop useful theory and improve macroeconometric model building. Applying Nell’s UMF to unemployment, inflation, and production reveals a methodological advance that promises more realistic insights into macroeconomic phenomena than is offered by contemporary, mainstream, econometric models.

Activity of Ligninolytic Enzymes during Growth and Fruiting Body Development of White Rot Fungi Omphalina sp. and Pleurotus ostreatus
HAPPY WIDIASTUTI,SUHARYANTO,AGUSTINA WULANINGTYAS,SUTAMIHARDJA
HAYATI Journal of Biosciences , 2008,
Abstract: The activity of ligninolytic enzymes of white rot fungi (WRF) Omphalina sp. and Pleurotus ostreatus was observed during somatic and fruiting body development in solid substrate fermentation using empty fruit bunches of oil palm (EFB). The enzyme activity was dominated by laccase both of Omphalina sp. and P. ostreatus. The laccase activity on somatic phase (mycelium growth) was higher compared to that of fruiting body formation phase. The laccase activity of Omphalina sp. was slightly higher compared to that of P. ostreatus. The peak activity of manganese peroxidase (MnP) for Omphalina sp. was observed two weeks after inoculation, while P. ostreatus had two peaks i.e. two and four weeks after inoculation period. The MnP activity of P. ostreatus was higher compared to that of Omphalina sp. Omphalina sp. growth in EFB did not secrete lignin peroxidase (LiP) in contrast to P. ostreatus. The peak of LiP activity of P. ostreatus was reached two and four weeks after inoculation. The MnP and LiP activities declined during the development of fruiting bodies while laccase increased both in Omphalina sp. and P. ostreatus. It seems that the activities of ligninolytic enzyme profile were regulated in line with developmental phase of growth both in Omphalina sp. and P. ostreatus.
ISOLASI SPESIES CANDIDA DARI TINJA PENDERITA HIV/AIDS
Mulyati,Retno Wahyuningsih,Widiastuti,Pudji K Sjarifuddin
Makara Seri Kesehatan , 2002,
Abstract: Candida is a saprophyte in the human respiratory tract, gastro intestinal tract and also in the debris under the nail. Inpatients with compromised immunity such as HIV-AIDS, Candida is able to cause infection, in this case oral candidosisor esophagitis. In this study fungi were isolated from the stools of HIV/AIDS patients. Samples consisting of 95diarrheic stools from HIV/AIDS patients were investigated for the yeast especially Candida spp. The stools were inoculated onto Sabouraud dextrose agar then the fungi were identified using morphological methods and Chromagarmedium. Yeast colonies were found in 71 (74,74%) out of 95 samples from which Candida was 42 44,21%), Geotrichum 24 (25,26%), and mixed of Candida and Geotrichum 3 (3,16%), Rhodotorula and Trichosporon 1(1,05%) each. Species of Candida were identified as C. albicans, C. tropicalis, C. krusei, C. guilliermondii, C. glabrata, C. lusitaniae and C. kefyr. Although Candida could be isolated from the diarrheic stools of HIV/AIDS patients but its role on the cause of diarrhea is still questionable.
Protein Patterns in Arbuscular Mycorrhizal Roots and Non-Mycorrhizal Roots of Oil Palm Seedling
HAPPY WIDIASTUTI,NAMPIAH SUKARNO,LATIFAH KOSIM DARUSMAN
Microbiology Indonesia , 2007,
Abstract: A comparison of the protein patterns in root extracts from none mycorrhizal and mycorrhizal oil palm rootshas been made. The polypeptides were analyzed every three weeks up to 11 weeks. A factorial design of fungi species (no mycorrhizal, Acaulospora tuberculata, Gigaspora margarita) and with or without fertilizer was assessed. The result showed that specific polypeptides were detected in primary and secondary roots. In unfertilized oil palm root, a 60 kDa polypeptide was detected while it was abcent in fertilized root. Inoculation of A. tuberculata with the addition of fertilizer application yielded a specific 26.7 kDa polypeptide in primary root on the 11th week after inoculation. A specific 64.2 kDa polypeptide of G. margarita was detected in unfertilized secondary root also on the 11th week.
Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics
ALFIN WIDIASTUTI,SOBIR,MUH RAHMAD SUHARTANTO
Nusantara Bioscience , 2010,
Abstract: Widiastuti A, Sobir, Suhartanto MR. 2010. Diversity analysis of mangosteen (Garcinia mangostana L.) irradiated by gamma-ray based on morphological and anatomical characteristics. Nusantara Bioscience 2: 23-33. The aim of this research was to increase genetic variability of mangosteen (Garcinia mangostana L.) irradiated by gamma rays dosage of 0 Gy, 20 Gy, 25 Gy, 30 Gy,35 Gy and 40 Gy. Plant materials used were seeds collected from Cegal Sub-village, Karacak Village, Leuwiliang Sub-district, Bogor District, West Java. Data was generated from morphological and anatomical characteristics. The result indicated that increasing of gamma ray dosage had inhibited ability of seed to growth, which needed longer time and decreased seed viability. Morphologically, it also decreased plant heigh, stem diameter, leaf seizure, and amount of leaf. Anatomically, stomatal density had positive correlation with plant height by correlation was 90% and 74%. Gamma rays irradiation successfully increase morphological variability until 30%. Seed creavage after irradiation increased variability and survival rate of mangosteen.
Analisis keragaman manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan karakteristik morfologi dan anatomi
ALFIN WIDIASTUTI,SOBIR MUH RAHMAD,SUHARTANTO
Bioteknologi , 2010,
Abstract: Abstrak. Widiastuti A, Sobir, Suhartanto MR. 2010. Analisis keragaman manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan karakteristik morfologi dan anatomi. Bioteknologi 7: 85-98. Tujuan penelitian ini adalah meningkatkan keragaman genetik manggis (Garcinia mangostana L.) yang diiradiasi dengan sinar gamma dosis 0 Gy, 20 Gy, 25 Gy, 30 Gy, 35 Gy dan 40 Gy. Bahan tanaman yang digunakan adalah biji yang dikumpulkan dari Kampung Cegal, Desa Karacak, Kecamatan Leuwiliang, Kabupaten Bogor, Jawa Barat. Data dihasilkan dari karakteristik morfologi dan anatomi. Hasil penelitian menunjukkan bahwa peningkatan dosis sinar gamma dapat menghambat pertumbuhan benih, sehingga membutuhkan waktu lebih lama untuk tumbuh dan menurunkan viabilitas benih. Secara morfologi, hal itu juga menurunkan tinggi tanaman, diameter batang, ukuran daun, dan jumlah daun. Secara anatomi, kepadatan stomata berkorelasi positif dengan tinggi tanaman dengan nilai korelasi adalah 90% dan 74%. Iradiasi sinar gamma dapat meningkatkan keragaman morfologi hingga 30%. Pemotongan benih setelah iradiasi dapat meningkatkan keragaman dan tingkat kelangsungan hidup manggis.
EFFECT OF TURMERIC EXTRACT ON BLOOD PARAMETERS, FEED EFFICIENCY AND ABDOMINAL FAT CONTENT IN BROILERS
Sugiharto,Isroli,E. Widiastuti,N. S. Prabowo
Journal of the Indonesian Tropical Animal Agriculture , 2011,
Abstract: The experiment was carried out to investigate the effect of turmeric extract on blood parameters, feed efficiency and abdominal fat content in broilers. A total of 100 mixed-sexes Lohman chicks were used in the experiment set up with completely randomized design of 5 different doses of turmeric extract given orally start from day-15 to day-35. The chicks given no turmeric extract were used as a control (T0), whereas T1, T2, T3 and T4 were the chicks given turmeric extract of 200, 400, 600 and 800 mg/kg-live BW, respectively. Hemoglobin values of broilers given turmeric extract 800 mg/kg-live BW was significantly higher (p<0.05) at week-3 and week-4, but not at week-5, compared to broilers given turmeric extract with lower doses or control group. Turmeric extract did not affect significantly (p>0.05) the concentration of erythrocytes, the levels of hematocrit as well as FCR and abdominal fat content of broiler. Turmeric extract seemed to be able to improve the digestion and metabolism (of fat) resulted in enhanced production of hemoglobin in broilers. The hypolipidemic effect of turmeric that could hamper its potential to promote the growth of broilers per se might explain why turmeric could not improve the feed efficiency of broiler.
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