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Search Results: 1 - 10 of 130814 matches for " WANG Yin-geng "
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Histopathology of turbot associated with Mesanophrys carcini parasite

QIN Lei,WANG Yin-Geng,ZHANG Li-Jing,DAI Ji-Xun,

水生生物学报 , 2007,
Abstract: Turbot,Scophthalmus maximus(L.) is a very important commercial species in Europe,from where it was introduced into China in 1992.With the success in artificial breeding from 1998,the commercial culture has spread rapidly along the coast of North China.Recently,yields have reached 20,000MT,valued at 200 million RMB.From 2003,a novel disease in turbot associated with Mesanophrys carcini infection was identified in China,causing high mortality and economic losses.This paper first reports the histopathology of this disease,aiming at supplying theoretical foundation for the prevention and therapy.In the case of infected larvae,ciliates could be found in fin,skin,gills,eye and internal organs such as brain,heart,kidney,spleen,pancreas,liver and the alimentary tract,which indicated the systemic infection to the larvae.In infected large turbot,the ciliates were predominantly found in the skin,fin and gills.And in the severe cases,they could invade the brain,liver,kidney and heart with little numbers.Histopathological studies revealed that various pathological changes to the tissues associated with Mesanophrys carcini.The affected larvae and large turbot showed the consistent histopathological changes,including hyperplasia of the branchial epithelium,meningitis,liquefaction of the brain,necrosis of haemopoietic tissue in the kidney and spleen,dilation of central vein and sinusoids in the liver,coagulative necrosis of muscle fibers,congestion and oedema of choroid in the eye and proliferation of mononuclear macrophages.Mesanophrys carcini caused damage to the gills,resulting in the asphyxia of the diseased turbot.This is probably the major causes of death in affected fish.And organs failure as a result of necrosis associated with presence of Mesanophrys carcini is also responsible for the death of affected fish.In addition,we discuss possible route of entry into the host,considering that injured skin and gills are the main route for the Mesanophrys carcini infection.The study tells us that keeping pond cleaning,avoiding scratching the fish surface are very necessary for the prevention of the Mesanophrys carcini infection;the therapy should include not only external treatment but also the internal.
Isolation and identification of the pathogentic bacterium causing red body from cultured Turbot Scophthalmus maximus

DONG Li,WANG Yin-geng,ZHANG Zheng,QU Jiang-bo,CHEN Xia,

海洋科学 , 2009,
Abstract: A dominant strain of the bacteria causing red body of turbot was isolated from the diseased turbot and designated as Hl.In an artificial infection test ,all fish of the experimental group died in 15 days after intramuscularly being injected with a high bacterial suspention (1.41×109CFU/mL) , and the low bacterial suspention (1.41 × CFU 103/mL) did not cause any death, while the control group showed no signs all through the experimentation. The LD50 was calculated as 2.82×105CFU/mL.The moribund experimental fish had gross signs similar to the natural infected fish. The bacterium re- isolated from the challenged fish also had the same characteristics as H1, which proved that the isolated H1 was the pathogenic bacterium that triggered this red body disease. Different methods were used to identify the pathogenic bacterium. The bacterium is negative, rod in shape, peritrichous flagella under electronic microscope. Traditional biochemical identification revealed that H1 exhibited relatedness to Edwardsiella tarda, while the identification result by API 32E system indicated that H1 was E. tarda with 99.9% reliability. Its 16S ribosomal RNA gene sequences were analyzed, and it is shown from the aniysis results that this strain of bacterium has a close phylogenetic relation (99%)to E.tarda. In summary, the pathogenic bacterium was identified as E. tarda.
Behavioral responses of sea cucumber (Apostichopus japonicus) to different light intensity and settlement substratum color

ZHANG Hui,WANG Yin-geng,RONG Xiao-jun,CAO Shan-mao,CHEN Xia,

生态学杂志 , 2009,
Abstract: 为了探讨刺参的趋光特性和附着基颜色对其行为反应的影响,分别用平均趋光率(mean phototactic rate, MPR)和平均聚集率(mean attractive rate, MAR)统计分析了刺参在100、50、10、5和1 lx共5档强度的光照和7种颜色的附着基(红色、灰色、黄色、蓝色、白色、黑色和绿色)下的行为反应差异。结果表明:在水平光场内,光源照度为100、50和10 lx时,光照度较强区与光照度较低区之间的MPR均存在显著差异(P﹤0.05),而光源照度为5和1 lx时,各区域之间的MPR无显著差异;不同颜色附着基实验时,刺参在黑暗和自然光照条件下,同种颜色附着基的MAR无显著差异(P>0.05);在相同光照条件下,黑色和灰色附着基的MAR明显优于其他颜色的附着基,与蓝色、黄色及不放置附着基的MAR差异极显著(P<0.01),不同颜色附着基的MAR由大到小排列为黑色>灰色>红色>绿色>白色>蓝色>黄色。表明刺参的趋光性随着光照强度的增加而减小,呈负趋光性;平均聚集率较高的3种颜色的附着基是黑色、灰色和红色。
Main diseases of cultured Apostichopus japonicus: prevention and treatment

WANG Yin-geng,RONG Xiao-jun,ZHANG Chun-yun,SUN Su-feng,

海洋科学 , 2005,
Abstract: Sea cucumber farming has been developed rapidly along the northern coast of China since the beginning of 21th century. It was estimated that the total culture area of sea cucumber has reached 33000 ha and the gross annual value has exceeded RMB 10 billion. The rapid expansion of sea cucumber farming has led to occurrence of various diseases, causing serious economic losses and becoming one of the limiting factors in the sustainable development of this industry. The authors carried out epidemiological study on the diseases of cultured sea cucumber Apostichopus japonicus; several diseases were reported for the first time, including ulcerated margin, ulcerated stomach, and autolysis of juveniles, in nursery stage. Among them the skin ulceration disease was the major one in out-door cultivation. Several types of pathogens were recognized, including bacteria, fungi and parasites, raptors and competi- tors could also result the disease. Treatment and preventative measures for these diseases were also proposed.
Annual changes of total heterotrophic bacteria and Vibrios in the intestine of Apostichopus japonicus and its culture pond

LI Bin,RONG Xiao-jun,LIAO Mei-jie,ZHANG Zheng,WANG Yin-geng,WANG Lan,LIU Zhi-chao,XUE Tai-shan,
李 彬
,荣小军,廖梅杰,张 正,王印庚,王 岚,刘智超,薛太山

海洋科学 , 2012,
Abstract: Annual changes of total heterotrophic bacteria and Vibrios in intestine of Apostichopus japonicus and its culture environment were studied from September 2008 to Novemver 2009. We found that the number of bacteria estimated by HPC (Heterotrophic Plate Count) and VBC (Vibrio bacterial Count) had significant seasonal changes in the intestine of A. japonicus and the cultured environment. HPC and VBC in the intestine of A. japonicus ranged from 1.85×105 to 2.17×109 CFU/g and from 4.1×104 to 2.2×108 CFU/g, respectively. HPC and VBC in cultured pond water ranged from 90 to 4.67×105 CFU/mL and from 0 to 5.3×103 CFU/mL, respectively. HPC and VBC in the pond sediment ranged from 9.80×104 to 6.72×106 CFU /g and from 1.01×104 to 5.75×105 CFU/g, respectively. HPC and VBC in cultch sediment ranged from 2.78×105 to 2.57×107 CFU /g and from 4.6×104 to 1.31×107 CFU/g, respectively. The total bacterial count in summer or autumn was higher than other seasons. The ratios of VBC to HPC were also calculated and indicated that the proportion of Vibros reached 43.8% and 43.2% in winter and spring. Although HPC in winter and spring were lower than thoese of summer and autum, the proportions of Vibro were higher in winter and spring than that of summer and autumn, potentially being the reason of high disease incidence during winter and spring. Our results could be useful to healthy culture of sea cucumber.
Bacteria community in the intestine and culture environment of Apostichopus japonicus in winter

LI Bin,RONG Xiao-jun,LIAO Mei-jie,CHEN Gui-ping,ZHANG Zheng,WANG Yin-geng,XUE Tai-shan,

海洋科学 , 2010,
Abstract: Studies on the bacteria community in the intestine and culture environment of Apostichopus japonicus in winter were conducted using tranditional bacterial cultivation method combined with molecular biology techniques during the period between November 2008 to January 2009. The total numbers of bacteria in the pond water, the sediment, the cultch and A. japonicus intestine, estimated by the tranditional bacterial cultivation method, ranged from 0.75×102 to 1.4×104 cfu/mL, 8.7×104 to 8.1×105 cfu/g, 3.8×105 to 2.8×106 cfu/g and 7.1×105 to 1.5×107 cfu/g, respectively. Twenty-two strains were isolated judged by the morphological characteristics of bacteria cultured on the plates. ARADA analysis with restriction enzymes Rsa I and Msp I revealed that the 22 strains were divided into 8 Operational Taxonomic Units (OTUs), which were dominated by OTU2 and OTU3 that accounted for 30% and 20% of the total isolated strains, respectively. Molecular identification was employed to identify the dominant bacteria in different environments. It was shown that the dominant bacteria were Pseudomonas stutzeri, P. mendocina, and Bacillus megaterium in the pond water; B. megaterium, P. stutzeri, and Bacillus thuringiensis in the sediment; B. megaterium, B. thuringiensis, and Vibrio splendidus on the cultch; B. megaterium, B. thuringiensis, V. splendidus, and P. stutzeri in the sea cucumber intestine. The result of this experiment may be helpful for hypothermia probiotics isolation and disease prevention in sea cucumber culture.
De novo transcriptome assembly of RNA-Seq reads with different strategies
Geng Chen,KangPing Yin,Charles Wang,TieLiu Shi
Science China Life Sciences , 2011, DOI: 10.1007/s11427-011-4256-9
Abstract: De novo transcriptome assembly is an important approach in RNA-Seq data analysis and it can help us to reconstruct the transcriptome and investigate gene expression profiles without reference genome sequences. We carried out transcriptome assemblies with two RNA-Seq datasets generated from human brain and cell line, respectively. We then determined an efficient way to yield an optimal overall assembly using three different strategies. We first assembled brain and cell line transcriptome using a single k-mer length. Next we tested a range of values of k-mer length and coverage cutoff in assembling. Lastly, we combined the assembled contigs from a range of k values to generate a final assembly. By comparing these assembly results, we found that using only one k-mer value for assembly is not enough to generate good assembly results, but combining the contigs from different k-mer values could yield longer contigs and greatly improve the overall assembly.
Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
Peng Wang, Yu Liu, Yonggang Yin, Haojie Jin, Shouxian Wang, Feng Xu, Shuang Zhao, Xiaoli Geng
International Journal of Biological Sciences , 2011,
Abstract: Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat to isolate and classify beneficial microorganisms that could affect its growth, which could be used in future research on artificial cultivation. In total, 342 isolates were isolated from soil samples collected around a C. rutilus colony in the Beijing region. Of these, 22 bacterial and 14 fungal isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Using 16S rRNA gene sequence analysis, the bacterial isolates were divided into two monophyletic clusters which had significant hits to the genera Bacillus and Pseudomonas, respectively. Using internal transcribed spacer (ITS) sequence analysis, fungal isolates were divided into four monophyletic clusters: Penicillium, Trichoderma, Mortierella, and Bionectria. Moreover, the phylogenetic diversity of these isolates was analysed. The results indicated that numerous microorganisms were present in C. rutilus habitat. This was the first reported examination of the microbiological ecology of C. rutilus.
Correlation between chemosensitivity to anticancerdrugs and telomerase reverse transcriptase mRNAexpression in gastric cancer
Lin Wang, Pei-Feng Li, Ming Geng, Yong-Cheng Cao, Ying-Chun Yin
Diagnostic Pathology , 2013, DOI: 10.1186/1746-1596-8-33
Abstract: The GC cells which were collected from 68 patients with primary GC were primary cultured. The chemosensitivity of GC cells to anticancer drugs was evaluated successfully using the MTT assay for 60 cases of GC cells, and the hTERT mRNA expression was examined in 60 cases of GC tissues and corresponding normal gastric mucosa and 6 cases of chronic superficial gastritis mucosa by in situ hybridization.Taxol, Cisplatin and 5-Fluorouracil were in general more effective than Adriamycin and Mitomycin for GC cells, and the chemosensitivity to anticancer drugs was associated with tumor histological types and a worse tumor grade. Compared to normal gastric mucosa tissues, hTERT mRNA expression was significantly increased in GC (P<0.05), which was related with a worse differentiation and drug-resistance to 5-Fluorouracil or Adriamycin in GC.These data demonstrate for the first time that examinations of hTERT mRNA expression as an important factor could be used to select the chemotherapeutic drugs for GC patients. Virtual slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1793217009875483
On the structure of AP-4 responsive element in the LTR of Jembrana disease virus
Zijing Liu,Hongyan Yin,Gang Deng,Yuhua Yuan,Jinzhong Wang,Yunqi Geng,Qimin Chen
Chinese Science Bulletin , 2003, DOI: 10.1007/BF03183946
Abstract: Previous studies with deletion and sequence analysis of JDV LTR showed that there is a putative AP-4 responsive element in LTR. By antisense transient assay and gel shifting assay, we for the first time demonstrated that AP-4 modulated JDV gene expression by binding DNA directly to bovine cells. The results, derived from site-directed mutagenesis experiments, suggest that the six base pairs of AP-4 binding site (CAGCTG) have different effects on JDV gene expression. When the first two base pairs changed to GC, JDV gene expression is severely decreased.
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