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Search Results: 1 - 10 of 133778 matches for " WANG Run-fang "
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Purification and properties of a thermostable chitinase from thermophilic fungus Thermomyces lanuginosus
疏绵状嗜热丝孢菌热稳定几丁质酶的纯化及其性质研究

GUO Run-fang,LI Duo-chuan,WANG Rong,
郭润芳
,李多川,王荣

微生物学报 , 2005,
Abstract: A thermostable extracellular chitinase from culture supernatant of a thermophilic fungus Thermomyces lanuginosus was purified to SDS-PAGE homogeneity, by using ammonium sulfate fraction, DEAE-Sepharose Fast flow chromatography, Phenyl-Sepharose Fast Flow chromatography. A molecular mass of the purified enzyme was between 48-49.8 kD determined by SDS-PAGE and gel filtration chromatography. The chitinase exhibited optimum catalytic activity at pH 4.5 and 55 degrees C respectively. It was thermostable at 50 degrees C and retained 24% activity after 20 min at 70 degrees C. The half life time of the enzyme at 65 degrees C was 25 min. Different metal ions showed different effects on the chitinase activity. Ca2+, Ba2+, Na+, K+ enhanced the enzyme activity, whereas Fe2+, Ag+, Hg2+, Cu2+ caused obvious inhibition. The Km and Vmax values of chitinase on colloidal chitin were 9.56 mg/mL and 22.12 micromol/min respectively. The chitinase showed antifungal activity aginst tested fungi to different degree.
Establishment of 3D Urban Landscape Model Based on Skyline Terrasuite
基于Skyline Terrasuite的城市三维景观的建立

DENG Jie,XIA Chun-lin,WANG Run-fang,
邓洁
,夏春林,王润芳

遥感技术与应用 , 2008,
Abstract: This paper takes the color photography picture and large-scale terrain map from Northeast University as data source,and gains the DOM data and DEM by using VirtuoZoNT3.5.After pretreatment,these data can have the same coordinate system.By using 3D GIS software Skyline Terrasuite,we overlay DOM to DEM structuring the vein coming to show 3D circumstances and the building roof 3D surface.Also by making use of a digital camera to collect the side vein of buildings,we can achieve 3D landscape of city Visualization.The paper discus above-mentioned method process,and give some experience summary.
Separation of noisy mixed image based on Wiener filtering and independent component analysis
基于维纳滤波和快速独立分量分析的有噪混合图像盲分离

LI Hong-yan,HAO Run-fang,MA Jian-fen,WANG Hua-kui,
李鸿燕
,郝润芳,马建芬,王华奎

计算机应用研究 , 2007,
Abstract: The basic mathematic model and algorithm of independent component analysis and the apply of independent component analysis in noisy mixed images was discussed, a method was proposed of combining Wiener filtering de-noising and independent component analysis. The results show that this method can reduce the affect of noise, accordingly renew the original image.
Teaching Reform and Practice of Microbiology Based on the Modern Education Concept
以现代教育观念为核心 积极开展微生物学教学改革与实践

GUO Run-Fang,YU Hong-Wei,HAN Jun,MA Wen,PEI Jia-Wei,WANG Xue-Jing,LIN Yang,ZHANG Wei,
郭润芳
,于宏伟,韩军,马雯,裴家伟,王雪静,林扬,张伟

微生物学通报 , 2010,
Abstract: The human-oriented education is the central idea of scientific development view.According to the characteristics of the bioengineering students,this paper presented the reform and practice on the teaching method of Microbiology,including guiding ideology,teaching content and teaching method.The teaching practice proved that the new pattern of Microbiology education not only stimulated students' interest in studying the course,but also developed their creative capability and the comprehensive quality.So it m...
A Classified Pre-Computed QoS Routing Algorithm
一种分类预计算QoS路由算法

FENG Jing,ZHOU Run-fang,GU Guan-qun,
冯径
,周润芳,顾冠群

软件学报 , 2002,
Abstract: In order to satisfy the quality of service (QoS) of the application on Internet, Internet engineering task force (IETF) has presented a set of network service mode such as IntServ/RSVP (Integrated Service/Resource Reservation Protocol), DiffServ (differentiated service) and MPLS (multi protocol label switching). All of these service modes need a correlative QoS routing mechanism and algorithm to assign an adapted route. Based on the research of existing routing algorithms, a new pre-computed QoS routing algorithm CCPF(classified minimum cost path first)is put forward,which uses bandwidth as a basic metric and gives a weighted cost function as a path optimal criterion.By simulation,this algorithm is compared with the others.The results show that it can get differet routes among different bandwidth-ranges to support multipl service modes.
PURIFICATION AND PROPERTIES OF β -GLUCOSIDASE FROM THERMOPHILIC CHEATOMIUM THERMOPHILE
一株嗜热毛壳菌β-葡萄糖苷酶的分离纯化及特性

CHU Chun-Xue,LI Duo-Chuan,GUO Run-Fang,
楚春雪
,李多川,郭润芳

菌物学报 , 2004,
Abstract: 研究了液体发酵嗜热毛壳菌Chaetomium thermophile产生的β-葡萄糖苷酶的分离纯化及特性。粗酶液经硫酸铵沉淀、DEAE-Sepharose Fast Flow阴离子层析、Phenyl-Sepharose 疏水层析、Sephacryl S-100分子筛层析等步骤后获得凝胶电泳均一的β-葡萄糖苷酶。经12.5%SDS-PAGE和凝胶过滤层析方法分别测得该酶的分子量大小约为78.4kDa和81kDa。该酶反应的最适温度和pH值分别为60℃和4.5-5.0。有较好的酸稳定性和热稳定性。金属离子对β-葡萄糖苷酶的活性影响较大, 其中Ca2+对酶有激活作用, 而Ag+、Cu2+ 、Hg2+对酶有显著的抑制作用。该酶对水杨苷具有很强的底物特异性。
Production and Stability of a Thermostable Chitinase
一种耐热几丁质酶的产生及其稳定性研究

GUO Run-Fang,SHI Xiao-Qin,LI Duo-Chuan,WEI Qing,JIA Ying-Min,
郭润芳
,史小琴,李多川,魏 青,贾英民

微生物学通报 , 2008,
Abstract: 疏绵状嗜热丝孢菌(Thermomyces lanuginosus)SY2 在以胶状几丁质为唯一碳源的诱导培养基中产生了胞外几丁质酶.该酶在50℃保温1 h,酶活稳定;65℃时半衰期为25 min;酶液在室温下保存到12周,残余酶活性为45%左右.该酶有较宽的pH范围,3.0~9.0之间保持稳定,pH值为2.5时,仍具有70%的剩余酶活性.Ca2 对几丁质酶的活性有显著的激活作用;高浓度变性剂对酶有抑制作用.结果表明该酶是一种热稳定性高且耐酸碱的新型几丁质酶,能在酸性和高温环境中发挥作用,这些特性赋予了T.lanuginosus几丁质酶在几丁质的生物转化及其它生物技术中极大的应用优势.
Production and Stability of a Thermostable Chitinase
一种耐热几丁质酶的产生及其稳定性研究

GUO Run-Fang,SHI Xiao-Qin,LI Duo-Chuan,WEI Qing,JIA Ying-Min,
郭润芳
,史小琴,李多川,魏 青,贾英民

微生物学报 , 2008,
Abstract: 疏绵状嗜热丝孢菌(Thermomyces lanuginosus)SY2在以胶状几丁质为唯一碳源的诱导培养基中产生了胞外几丁质酶。该酶在50℃保温1 h, 酶活稳定; 65℃时半衰期为25 min; 酶液在室温下保存到12周, 残余酶活性为45%左右。该酶有较宽的pH范围, 3.0~9.0之间保持稳定, pH值为2.5时, 仍具有70%的剩余酶活性。Ca2+ 对几丁质酶的活性有显著的激活作用; 高浓度变性剂对酶有抑制作用。结果表明该酶是一种热稳定性高且耐酸碱的新型几丁质酶, 能在酸性和高温环境中发挥作用, 这些特性赋予了T. lanuginosus几丁质酶在几丁质的生物转化及其它生物技术中极大的应用优势。
Effect of Trichoderma aureoviride 1010 on chilly stress-resistance of tomato in solar-greenhouse conditions
黄绿木霉T1010对日光温室番茄耐冷生理指标的影响

CHEN Wei-Jing,LI Run-Fang,YANG Huan-Ming,CHEN Jian-Ai,
陈为京
,李润芳,杨焕明,陈建爱

中国生态农业学报 , 2010,
Abstract: In this study, we determined the enhancement effects of Trichoderma aureoviride 1010 (T1010) on chilly stress-resistance of tomato in solar-greenhouse conditions via soil application and 3 days of inferior chilly-stress (16 ± 2 °C in the day and 8 ± 2 °C at night ). Results show significant differences in the indicators for inferior chilly stress-resistance of functional tomato leaves and roots. SOD, CAT, POD and PPO activities of functional tomato leaves treated with T1010 increase by 266.33%, 16.85%, 20.89% and 632.89% respectively over the control. Carotenoid, ABA, proline, glucose and starch contents also increase by 50.53%, 119.40%, 55.38%, 128.57% and 23.40% respectively. For tomato roots treated with T1010, SOD, CAT, POD and PPO activities increase by 274.10%, 69.06%, 8.26% and 164.14% respectively. The contents of proline, glucose, and starch also increase by 49.81%, 66.00% and 86.76% respectively. In addition to decreasing soluble sugar content, the indicators for functional tomato leaves treated with 1/2T1010 increase at varying degrees. However, the indicators for tomato roots treated with 1/2T1010 decrease at varying degrees. ANOVA analysis shows significant difference in indicators of inferior chilly stress-resistance among CK, T1010 and 1/2T1010 treatments. T1010 positively influences chilly stress-resistance of functional tomato leaves and roots in solar-greenhouse conditions with reasonable soil organic quality.
The Application of Reverse-Time Migration in the Z Area of PRM Basin  [PDF]
Run He, Yuan Fang, Yanchun Wang, Lifang Cheng, Xi Zheng
International Journal of Geosciences (IJG) , 2016, DOI: 10.4236/ijg.2016.76060
Abstract: Reverse time migration (RTM) is an accurate migration method, which is based on two-way wave equation, eliminates angle limitation, and can be capable for imaging of reverse branch and multiple. In the development of an oilfield in Pearl River Mouth (PRM) Basin, RTM has been applied to solve the problems of fault shadow and structure in distinction and the drilling has proven that the RTM result is reliable. This paper introduces the theory of RTM and emphatically discusses parameter selection. The results of RTM showed that it had advantages in resolving fault shadow and the imaging of steep dip structure, and could be utilized in the oilfield.
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