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Search Results: 1 - 10 of 173256 matches for " WANG Kang-Yu "
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Transformation of large DNA fragment of Panax ginseng into Ganoderma lucidum

PANG Chun-Mei,ZHANG Liang,SUN Chun-Yu,JIANG Shi-Cui,WANG Kang-Yu,ZHANG Mei-Ping,WANG Yi,

菌物学报 , 2013,
Abstract: 为探讨人参大片段DNA转化灵芝的可能性,通过电击法将双元细菌人工染色体(BIBAC)载体上的100kb人参大片段DNA转化到灵芝原生质体内。研究发现,在电极间距为4mm,电压强度为240V时,将5μL的人参大片段DNA转化到75μL的灵芝原生质体,在选择培养基上获得了具有再生能力的转化子。根据克隆载体两侧的序列设计两对引物,对转化子进行PCR分析。试验结果表明人参大片段DNA已经转化到灵芝的基因组中。
Diverse functions of miR-125 family in different cell contexts
Sun Yu-Meng,Lin Kang-Yu,Chen Yue-Qin
Journal of Hematology & Oncology , 2013, DOI: 10.1186/1756-8722-6-6
Abstract: MicroRNAs (miRNAs) are emerging as a novel class of non-coding RNA molecules that regulate gene expression at a post-transcriptional level. More than 1000 miRNAs have been identified in human cells to date, and they are reported to play important roles in normal cell homeostasis, cell metastasis and disease pathogensis and progression. MiR-125, which is a highly conserved miRNA throughout diverse species from nematode to humans, consists of three homologs hsa-miR-125a, hsa-miR-125b-1 and hsa-miR-125-2. Members of this family have been validated to be down-regulated, exhibiting its disease-suppressing properties in many different types of diseases, while they also have disease-promoting functions in certain contexts. MiR-125 targets a number of genes such as transcription factors, matrix-metalloprotease, members of Bcl-2 family and others, aberrance of which may lead to abnormal proliferation, metastasis and invasion of cells, even carcinomas. Furthermore, miR-125 plays a crucial role in immunological host defense, especially in response to bacterial or viral infections. In this review, we summarize the implication of miR-125 family in disease suppression and promotion, focusing on carcinoma and host immune responses. We also discussed the potential of this miRNA family as promising biomarkers and therapeutic targets for different diseases in future.
Epigenetic Regulation of EpCAM in Tumor Invasion and Metastasis
Shine-Gwo Shiah,Kang-Yu Tai,Cheng-Wen Wu
Journal of Cancer Molecules , 2008,
Abstract: Metastatic progression is the cause of most cancer decease. Many cell surface adhesion molecules are known to be present or re-expressed following gene promoter CpG island hypomethylation in the early stage of growing tumors, but absent or reduced by gene promoter CpG hypermethylation in metastasized carcinomas. Recent studies have revealed that an adhesion receptor, epithelial cell adhesion molecule (EpCAM), mediates cell-cell interaction and is involved in tumor invasion and metastasis. EpCAM expression was associated with promoter CpG methylation in lung adenocarcinoma. Treatment with a demethylating agent and histone deacetylase inhibitor reactivated EpCAM expression in EpCAM-negative cells and inhibited cancer cell invasiveness. Covalent histone tail modifications of histone H3 lysine 9 (H3K9), including acetylation or methylation, regulate these different states of chromatin configuration and gene transcription. Methylated and deacetylated H3K9 was present in the silenced EpCAM gene promoter of highly invasive cells, but it was largely decreased in the activated EpCAM gene promoter in low invasive cells. Despite EpCAM immunotherapy has been tested as a treatment modality for numerous cancers, the tumor-inhibitory mechanisms of anti-EpCAM antibodies still remain controversial. Since dynamic change of EpCAM expression is a regulatory event in the process of invasion or metastasis and DNA methylation is one of the major mechanisms in regulating EpCAM expression during this process, hypermethylated EpCAM gene promoter may serve as an invasiveness and progression marker for cancer diagnosis, prevention and treatment.
Research of attack technologies for Web service

HUANG Kang-yu,HE Zheng-qiu,LAI Hai-guang,WU Li-fa,

计算机应用研究 , 2010,
Abstract: Web service greatly facilitates the application-to-application integration based on heterogeneous platform, but its core components are faced with threats of malicious attacks. This paper detailedly analyzed the principles and characteristics of various familiar attacks on single Web service and Web service composition, and pointed out the corresponding detection and prevention countermeasures. On the basis of current research achievements, also presented a discussion on the future research directions and the challenges of Web service defenses against attacks.
Salivary Amino Acids Determination and Their Changes in Vision Stress Experiments  [PDF]
Wei Tang, Xuesong Li, Xiaoxiao Wu, Yu Wang, Xuejun Kang
Open Journal of Social Sciences (JSS) , 2013, DOI: 10.4236/jss.2013.16005
Abstract: Amino acid neurotransmitters represent a major class of compounds that are involved in neuronal communication at CNS synapses, which can provide the basis for a variety of disease diagnosis and treatments and the study of the mechanism of mental illness. An analytical method for the determination of several amino acids in saliva was established with reversed-phase high performance liquid chromatography (RP- HPLC) with UV detector. About ten kinds of amino acids were detected in saliva. Nine subjects have participated in the stress experiments which have undergone a 50-min three-dimensional cartoon watching. The result of the experiment has proved that four kinds of salivary amino acids respond to the vision stress experiment obviously
Qiu He,Kang An,Peng Wang,Peng Yu
Acta Crystallographica Section E , 2010, DOI: 10.1107/s160053681002221x
Abstract: The title compound, C16H12FN3OS, was synthesized by the reaction of 5-(4-methoxyphenyl)-1,3,4-thiadiazol-2-amine and 4-fluorobenzaldehyde. An intramolecular C—H...S hydrogen bond results in the formation of two five-membered rings. In the crystal structure, intermolecular C—H...N hydrogen bonding links the molecules, forming a two-dimensional network.
Characterizations and Analysis of the Mold from Animal Specimens in Shenzhen Museum  [PDF]
Rui Zhang, Kang Wang, Meirong Sun, Bo Zhang, Honglian Zhang, Sidong Li, Hui Du, Yanlin Lu, Yang Ye, Xiaoqiang Yu
Journal of Environmental Protection (JEP) , 2016, DOI: 10.4236/jep.2016.77091

Biological specimens play an important role in cultural exchange, science popularization, scientific research and economic window, but the preparation and preservation technology system of biological specimens is relatively unsafe and inefficient. Mold grows seriously on animal specimens, which is not only harmful to human beings’ health and environment, but also is one of the factors that restricts the development of the natural history museums where these specimens are kept. This paper identified the mold species of animal specimens by PCR with ITS primers, bio-micro-scopic observation, sequencing and phylogenetic tree analysis. The results showed the mold of animal specimens mainly belonged to Aspergillus and Neurospora. This study established the foundations of controlling and restoring the mold that infected animal specimens and guided a new methodology of preparation and environmental friendly exhibition for animal specimens.

Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application
Qian Wang, Hongmin Yu, Yongzhen Xia, Zhen Kang, Qingsheng Qi
Microbial Cell Factories , 2009, DOI: 10.1186/1475-2859-8-47
Abstract: The successful expression of PHB biosynthesis and PHB depolymerase genes in E. coli was confirmed by PHB production and 3-hydroxybutyrate secretion. Starvation experiment demonstrated that the complete PHB mobilization system in E. coli served as an intracellular energy and carbon storage system, which increased the survival rate of the host when carbon resources were limited. Stress tolerance experiment indicated that E. coli strains with PHB production and mobilization system exhibited an enhanced stress resistance capability.This engineered E. coli with PHB mobilization has a potential biotechnological application as immobilized cell factories for biocatalysis and biotransformation.A wide variety of microorganisms are able to accumulate polyhydroxyalkanoates (PHAs) as intracellular carbon/energy storage compounds or reducing power for coping with changing, often oligotrophic environments [1,2]. Various PHAs, as well as the best-known poly 3-β-hydroxybutyrate (PHB), were found to be accumulated and degraded as required under environmental conditions by most natural PHAs producing bacteria [3]. When the environment is sufficient with carbon source or the C/N ratio is quite high (>20), the PHAs accumulation is much faster than degradation [4,5]. While facing different stresses, such as low nutrient availability and detrimental physical, chemical, or biological factors, these bacteria begin to mobilize PHAs to conquer those unfavorable environments. The biosynthesis and degradation of PHAs is a cyclic mechanism that has already been found in many bacteria, such as Ralstonia eutropha, Azotobacter beijerinckii and Hydrogenomonas eutropha [6-8]. The in vivo PHB biosynthesis pathway is conducted by the successive action of β-ketoacyl-CoA thiolase (phbA), acetoacetyl-CoA reductase (phbB) and PHB polymerase (phbC). However, PHB degradation, which has been investigated for years, was divided into intracellular mobilization and extracellular degradation. Intracellular PHB mo
Synthesis and Microwave Absorbing Properties of FeCoNi Alloy Particles/Graphite Flaky Composites
WANG Chen,KANG Fei-Yu,GU Jia-Lin
无机材料学报 , 2010, DOI: 10.3724/sp.j.1077.2010.00406
Abstract: Exfoliated graphite was separated into nanosized flakes by ultrasonication and acid treatment, and then FeCoNi alloy particles were deposited uniformly on the surface of flakes by codeposition method and annealing treatment process. Magnetic and microwave absorbing properties of the obtained samples were measured. The results show that the synthesized composites possess good soft magnetic performance and effective microwave absorption. The composite consisting of Fe3Co6Ni/graphite flakes annealed at 600℃ as an absorber has the maximum absorption value of -24dB at 12.6GHz, and the effective absorbing bandwidth (<-5dB) is about 8GHz. The characteristics of microwave absorption can be controlled by adjusting element ratios in alloy and the parameters of heat treatment process. This kind of composite has great potential and investigation value in microwave absorbing fields.
Poor Metabolizers at the Cytochrome P450 2C19 Loci Is at Increased Risk of Developing Cancer in Asian Populations
Hong Wang, Kang Song, Zenggan Chen, Yanmin Yu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073126
Abstract: Background CYP2C19 encodes a member of the cytochrome P450 superfamily of enzymes, which play a central role in activating and detoxifying many carcinogens and endogenous compounds thought to be involved in the development of cancer. In the past decade, two common polymorphisms among CYP2C19 (CYP2C19*2 and CYP2C19*3) that are responsible for the poor metabolizers (PMs) phenotype in humans and cancer susceptibility have been investigated extensively; however, these studies have yielded contradictory results. Methods and Results To investigate this inconsistency, we conducted a comprehensive meta-analysis of 11,554 cases and 16,592 controls from 30 case-control studies. Overall, the odds ratio (OR) of cancer was 1.52 [95% confidence interval (CI): 1.23–1.88, P<10-4] for CYP2C19 PMs genotypes. However, this significant association vanished when the analyses were restricted to 5 larger studies (no. of cases ≥ 500 cases). In the subgroup analysis for different cancer types, PMs genotypes had an effect of increasing the risks of esophagus cancer, gastric cancer, lung cancer and hepatocellular carcinoma as well as head neck cancer. Significant results were found in Asian populations when strati?ed by ethnicity; whereas no significant associations were found among Caucasians. Strati?ed analyses according to source of controls, significant associations were found only in hospital base controls. Conclusions Our meta-analysis suggests that the CYP2C19 PMs genotypes most likely contributes to cancer susceptibility, particularly in the Asian populations.
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