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Search Results: 1 - 10 of 126 matches for " WAHYU IRAWATI "
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A Study on Mercury-Resistant Bacteria Isolated from a Gold Mine in Pongkor Village, Bogor, Indonesia
WAHYU IRAWATI,PATRICIA,YENNY SORAYA,ABYATAR HUGO BASKORO
HAYATI Journal of Biosciences , 2012,
Abstract: Mercury is one of the major pollutant in the environment which is highly toxic. Bioremediation strategies using bacteria have been proposed as an attractive alternative because this is effective, less expensive and more efficient to remove mercury. Brevundimonas sp. HgP1 and Brevundimonas sp. HgP2 were two highly mercury resistant bacteria isolated from a gold mine in Pongkor village with MIC of 575 ppm. The purposes of the research were to study the effect of mercury on bacterial growth and morphological changes of bacterial colony and to measure the ability of bacterial isolates to accumulate Hg2+. The growth was monitored by measuring optical density at 600 nm, whereas accumulation of Hg2+ was measured by mercury vaporation unit. This present studies revealed that the addition of 50 and 100 ppm HgCl2 in Brevundimonas sp. HgP1 resulted in the decreasing of growth rate and the elongation of lag phase in 8 and 16 hours, respectively. The addition of HgCl2 also affected morphological appearance of the bacterial colony to black. Brevundimonas sp. HgP1 accumulated Hg2+ up to 1.09 and 2.7 mg/g dry weight of cells and removed 64.38 and 57.10% Hg2+ from the medium containing 50 and 100 ppm HgCl2, respectively.
Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia
WAHYU IRAWATI,TRIWIBOWO YUWONO,JOEDORO SOEDARSONO,HARI HARTIKO
Microbiology Indonesia , 2012, DOI: 10.5454/mi.6.3.3
Abstract: Copper resistant bacteria can be isolated from environments where copper levels are abundant from mining, industrial, or agricultural activities. The aim of this work was to study the molecular and physiologicalcharacteristics of indigenous copper resistant bacteria isolated from activated sludge in an industrial wastewatertreatment plant in Surabaya, Indonesia. The bacterial isolates were designated as strains IrC1, IrC2, and IrC4. Phylogenetic analysis based on 16S rDNA sequence analysis identified isolates IrC1, IrC2, and IrC4 as Acinetobacter oleivorans (98.41% similarity), Acinetobacter pitii (97.22% similarity), and Cupriavidus pauculus (96.99 similarity), respectively. The addition of 5 mM of CuSO4 in the medium affected morphological 4 appearance of all isolates to green and undulate margin might be due to the survival mechanism of bacteria by absorbing the copper. This studies indicated that copper resistance mechanism of all isolates was facilitated through the bioaccumulation of copper inside the cell, especially on the membrane fraction and inside the cytoplasm, albeit at a limited amount. It was observed that isolates IrC1, IrC2, and IrC4 were capable of accumulating 137.23 , 364.66 , and 272.07 mg L-1 of copper, respectively from the medium containing 8 mM CuSO4. The capability of isolates IrC1, IrC2, and IrC4 to accumulate copper can be exploited in bioremediation 4 process for removing copper from industrial sewage.
Feeding studies of radiation sterilization ready to eat foods on sprague dawley rats: In vivo  [PDF]
Zubaidah Irawati, Yulvian Sani
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.42017
Abstract: Development of gamma irradiated high moisture traditional dishes derived from locally traditional récipe aimed for specific target groups as ready to eat foods for ensuring the safety, quality, and security purposes have been conducted. The formation of free radicals in the traditional foods induced by ionizing radiation is a part of toxicological studies on irradiated traditional foods was to provide local scientific data base on safe- ty issues.The different foods such as steamed gold fish, spicy curry beef and soy sauces beef were individually vacuum packed in a laminate pouch of PET 12 μm/LDPE 2 μm/AL-Foil 7 μm/LDPE 2 μm/LLDPE 50 μm (PET/Al-Foil/ LLDPE), then kept at frozen state. The frozen samples were maintained in cryogenic condition along the irradiation process by placing the samples in styrofoam boxes filled with dry ice then irradiated with gamma rays at the dose of 45 kGy. The irradiated samples were kept and stored at normal temperature prior to test. Both irradiated conventional rat’s feed at normal temperature with the dose of 4 kGy and unirradiated one as control were also made. Irradiated and unirradiated samples were sent to animal laboratory, and fed into individual female Sprague Dawley rat as member of a group. The parameters observed were body weight changed of rats, toxicological test to observe the effect free radicals formation in rat’s blood by using malon-dialdehyde (MDA) and superoxide dismutase (SOD) methods, respectively, and anatomy pathology diagnosis. Different types of the foods sterilized by ionizing radiation fed ad libitum to the individual Sprague Dawley rat demonstrated that such foods did not give any adverse effect on the reduction of body weight, the toxicological impact, nor anatomy-pathology examinations of the rats.
Pediatric Retinal Detachment in Indonesia: Clinical Characteristics, Risk Factors, and Treatment Outcomes  [PDF]
Irawati Irfani, Arief S. Kartasasmita
Open Journal of Ophthalmology (OJOph) , 2017, DOI: 10.4236/ojoph.2017.74033
Abstract:
Purpose: To describe the clinical features and risk factors of pediatric retinal detachment among patients in Indonesia. Methods: This is a retrospective study involving 46 eyes of 34 children (younger than 18 years) diagnosed with pediatric retinal detachment. A detailed history was taken and a complete ophthalmic examination and a systemic examination were performed as required. Clinical characteristics, risk factors, and treatment choices were noted. Retinal detachment was categorized as tractional, exudative, or rhegmatogenous. Results: Mean patient age was 8.5 years (range, 0–18 years). Most patients (70%) were boys. Twelve (35%) patients had bilateral involvement at presentation. Tractional retinal detachment was found in 17 eyes (37%) and in this study was caused by retinopathy of prematurity (grade IV-V) in all cases. Exudative retinal detachment was found in 12 eyes (26%), the most common causes of which were panuveitis and Coat’s disease (both 50%). Rhegmatogenous retinal detachment was found in 17 eyes (37%), the most common risk factor for which was trauma (58%). Conclusions: Different approaches are needed to treat pediatric retina detachment in patients with different risk factors. Recognition of risk factors and early management will help to prevent childhood blindness due to retinal detachment.
Endoscopic Right Lobectomy Axillary-Breast Approach: A Report of Two Cases
Nina Irawati
International Journal of Otolaryngology , 2010, DOI: 10.1155/2010/958764
Abstract: Background. We reported our two initial experiences in the treatment of thyroid disease with endoscopic thyroidectomy. Minimally invasive video-assisted technique (MIVAT) was initially introduced by Miccoli. The modification was made by using axillary and breast technique. Method. Two young women patients, with 4 and 5 cm right lobe thyroid disease suspected to be benign. From physical examination, sonography, and FNAB findings, the two cases were categorized as benign. We performed endoscopic right lobectomy through incision of 5–10 mm on axillary line and breast using CO2 insufflation. Result. Duration of first operation was 300 minutes and the second one was 120 minutes, with minimal blood loss and no major complication. Patients were discharged 24 hours after operation. Cosmetic result was excellent. Postoperative complications were shoulder discomfort and neck swelling. Conclusion. We reported two cases of endoscopic right lobectomy as a safe, reproducible technique with an indication in a minority of patients candidates for thyroidectomy and is characterized by less postoperative discomfort.
PERKEMBANGAN DAN PROSPEK PROSES RADIASI PANGAN DI INDONESIA [Development and Prospect of Food Radiation Processing in Indonesia]
Zubaidah Irawati
Jurnal Teknologi dan Industri Pangan , 2008,
Abstract: Several factors such as insufficient harvesting and handling methods as well as inadequate methods of storage and distribution, poor processing techniques and poor quality of raw materials used in making ready to eat foods may lead to the cumulative causes of food borne illness particularly in developing countries. Public trend in the world nowadays are demanding access to more and more fresh eating products practical but nutritious, safe and preferably processed under non thermal treatments. The new and emerging post harvest technologies in controlling pathogen and maintaining quality of food products is ionizing radiation, because it is applicable for almost all type of foods without impairing the overall quality as well as sensory attributes. The foods either fresh, dried, or ready to eat meals in the packages can be exposed to ionizing radiation for different purposes such as quarantine measures, control of sprouting and germination, shelf-life extension of perishable foods, delaying ripening and aging of fruits and vegetables, destruction of parasites and harmful pathogenic microorganisms. International trade of agricultural commodities opens the possibility of the movement of pests such as insects from country to country. The countries involve in this business have established laws and regulations, including international trade regulation of irradiated foods, in order to minimize the risk and trade barrier. The future of food irradiation is filled with promise although the needs for this technique relates to consumer acceptance. Consumers will grow to appreciate the technology for the lifesaving and good food availability. It should be kept in mind that irradiation is controlling contamination and it does not prevent it.
Endoscopic Right Lobectomy Axillary-Breast Approach: A Report of Two Cases
Nina Irawati
International Journal of Otolaryngology , 2010, DOI: 10.1155/2010/958764
Abstract: Background. We reported our two initial experiences in the treatment of thyroid disease with endoscopic thyroidectomy. Minimally invasive video-assisted technique (MIVAT) was initially introduced by Miccoli. The modification was made by using axillary and breast technique. Method. Two young women patients, with 4 and 5?cm right lobe thyroid disease suspected to be benign. From physical examination, sonography, and FNAB findings, the two cases were categorized as benign. We performed endoscopic right lobectomy through incision of 5–10?mm on axillary line and breast using CO2 insufflation. Result. Duration of first operation was 300 minutes and the second one was 120 minutes, with minimal blood loss and no major complication. Patients were discharged 24 hours after operation. Cosmetic result was excellent. Postoperative complications were shoulder discomfort and neck swelling. Conclusion. We reported two cases of endoscopic right lobectomy as a safe, reproducible technique with an indication in a minority of patients candidates for thyroidectomy and is characterized by less postoperative discomfort. 1. Introduction Neck surgery is one of the newest and most interesting application of minimally invasive surgery technique in thyroid surgery, particularly with regard to eliminating the unattractive scars [1, 2]. It is well known that conventional thyroidectomy allows for prompt postoperative recovery. In some clinical settings, it is performed as an outpatient procedure. Findings have shown that video-assisted and endoscopic procedures for thyroid surgery have some advantages over conventional surgery in term of cosmetic result and postoperative recovery. These results support the development of endoscopic and video-assisted thyroid surgery. It should be emphasized that these procedures are technically demanding and require a surgical team skilled in both endocrine and endoscopic surgery. This is particularly true for some endoscopic techniques such as endoscopic thyroidectomy by breast or axillary approach. The endoscopic and video-assisted procedure requires a significant learning period, which can be time consuming especially at the beginning of a surgeon’s experience [3]. Minimal-access thyroid surgery was conceived primarily in Europe and Asia [1]. Endoscopic neck surgery for the parathyroid and thyroid was developed by Gagner and Huscher in 1996 and 1997, respectively. Since then, various methods including axillary, breast, and anterior chest approaches have been introduced by many surgeons. The use of endoscopic for complete thyroidectomy has been
Penerapan Model MIMIC untuk Menguji Konsistensi Hasil Pengukuran melalui Skala
Wahyu Widhiarso
Journal of Education and Learning , 2012,
Abstract: This study aimed to demonstrate the use of multiple indicators and multiple (MIMIC) model in testing the consistency of the measurement results when it's applied to individuals with different characteristics. Self-Esteem and Student Motivation Scale were employed to measure participant attributes. Impact of three student characteristics (gender, intelligence and school location) on both measurement model were examined. Total of 2981 students from 30 cities of 15 provinces were participated in this study. Data analysis was utilized using confirmatory factor analysis under structural equation modeling (SEM) approach. Results showed that measuring self-esteem was prone to be contaminated by individual heterogeneity. However, contrary results was found on motivation measure. Excluding three student characteristics as covariance in the model have increased model fit indices in the self-esteem measurement model, but not in the self-esteem. The presence of susceptibility scale to different characteristics indicated that this scale needs to be further modified. Therefore, when it is applied to heterogeneous population, the measurement results will produce consistently results.
Abundance and natural food resources of birds in Manusela National Park, Seram, Central Mollucas
WAHYU WIDODO
Biodiversitas , 2006,
Abstract: The aim of this research was to know the abundance and natural food resources of birds in the Manusela National Park, Seram (Ceram), Central Moluccas. The observations were done by “line-transect methods”, which in observe 70.50 hours totality. The results were found 51 species of birds in area of the national parks and 4 of all, namely red lory (Eos bornea), papuan hornbill (Rhyticeros plicatus), shining starling (Aplonis metallica), and grey-necked friarbird (Philemon subcorniculatus) were abundant. Fourty seven species of plants were known useful for 19 species of birds as the natural food resources, nesting-sites and roosting trees.
Population Status of Cacatua sulphurea parvula and Trichoglossus euteles in Alor
WAHYU WIDODO
Biodiversitas , 2009,
Abstract: The aim of this survey was to know the abundance status of the Yellow-crested Small Cockatoo (Cacatua sulphurea parvula) and Yellow-headed Lorikeet (Trichoglossus euteles) in Alor, East Nusa Tenggara Province. There were four sites observation in the survey, namely: (i) Air Mancur preparatory Village (in and around of Tuti Adigae Natural Tourism Park, East Alor Sub-district), (ii) Tanglapui Village (Kampong Irawuri) (East Alor Sub-district), (iii) Probur Village (Kampong Wormanem) (Southwest Alor Sub-district), and (iv) Bota Village (Northwest Alor Sub-district). The survey was used by bird encounter rates per unit time method. The population of birds approached by abundance score value. Fifty species of birds or 64.9% of 77 species of birds that found in Alor recorded during field working. The yellow-headed lorikeet observed more abundant with five abundance score and the numbers per 10 hours observation were 58.06. The yellow-crested small cockatoo no one recovered during in the survey. However, the yellow-crested small cockatoo knows in the fields in direct observation nesting in the old nest site of Canarium trees.
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