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Search Results: 1 - 10 of 84412 matches for " W. Wongkham "
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Genetic Structure and Diversity of the Giant Frog (Limnonectes blythii) in Northern Thailand
C. Suwannapoom,W. Wongkham,N. Sitasuwan,C. Phalaraksh
Research Journal of Applied Sciences, Engineering and Technology , 2012,
Abstract: The aim of this study is to analyse genetic diversity, structure and differentiation of the giant frogs (Limnonectes blythii). One hundred and sixty four individuals from 4 populations in Mae Hong Son Province, Thailand were used for the analysis of genetic polymorphism at 7 microsatellite loci. The collection showed considerable polymorphism with observed number of alleles per locus ranging for seven different loci, with an average of 3.4 alleles per locus. Mean genetic diversity of the four populations with moderate level, but in populations with lower genetic diversity. Furthermore, the NJ tree approach clustering conWrmed the results of PAM is more differentiated than the others. The signiWcant levels of genetic structure among the sites were found in which could be resulting from isolation by distance rather than a position relative to habitat. The results of this study indicate that genetic structure could be useful for evaluation of neutral genetic variation particularly as the basis for inferring population and species capacity for species conservation and management decisions.
Evaluation of Hatchery Stock of Giant Frog (Limnonectes blythii) in Thailand
C. Suwannapoom,W. Wongkham,N. Sitasuwan,M. Osathanunkul
International Journal of Animal and Veterinary Advances , 2012,
Abstract: The aims of this work were to study the relationship between temperature and hatching rate and to evaluate genetic diversity of giant frog (Limnonectes blythii) as parental stock in Mae Hong Son Inland Fisheries Station, northern Thailand. The results showed that the optimal temperature for development of giant frog hatching ranged from 19 to 27oC, based on total hatch rate, viability of newly hatched larvae post-hatch, and total mortality rate of eggs. Moreover, from November to February, the greatest percentage of hatching was in January, with 40%. The outcome of determining the genetic diversity of parental generation by using seven microsatellite loci was moderately high levels.
Phylogenetic Relationship of Limnonectes (Anura: Dicroglossidae) in Thailand
C. Suwannapoom,W. Wongkham,N. Sitasuwan,S. Chomdej
Current Research Journal of Biological Sciences , 2012,
Abstract: The aims of this study was to relationship between systematic of fanged frogs are assessed by using the nuclear POMC gene from twenty three samples. It is suggested that there are three monophyletic groups in which the L. gyldenstolpei, L. hascheanus and L. limborgi were clustered together (group I); L. blythii (group II) and L. taylori, L. jarujini and L. megastomias (group III). Sequence analyses indicated that Limnonectes in Thailand has genetic diversity (h = 0.96, π = 0.045). Analysis of genetic distance on the basis of sequence differences for the POMC gene showed significant genetic difference data from phylogenetic analysis and revealed a very large genetic difference between species for the POMC gene sequences. It was also confirmed that the POMC gene is an effective unit for studying relationships in Limnonectes species.
Phenotypic Characteristics and Function of NK Cell Subsets in cART-Treated HIV-1-Infected Individuals  [PDF]
Manthana Mitchai, Niramon Leeratanapetch, Viraphong Lulitanond, Pattaravadee Srikoon, Shinichiro Hattori, Kulthida Vaeteewoottacharn, Sopit Wongkham, Seiji Okada
World Journal of AIDS (WJA) , 2014, DOI: 10.4236/wja.2014.43034

Natural killer (NK) cell plays an important role in an innate immune response against viral infection. The kinetics regulation and functional consequences of NK cells in the pathogeneses of diseases are uncertain. We analyzed NK cell distribution and function of successfully combination antiretroviral therapy (cART)-treated HIV-1 infected individuals in Khon Kaen Regional Hospital, Thailand. The results demonstrated that increased percentage and the total number of NK cell in cART-treated HIV-1 infected patients with preferential high levels of CD56dimCD16+ and CD56-CD16+ subsets when compared with a control group even in undetectable viral load (<40 copies per milliliter). Concomitantly, decreased cytotoxic activity measured by CD107asurface expression with maintained IFN-γ production implied the impairment of cytolytic activity was not recovered after cART treatment. Thus, altered NK cell frequency and function by HIV-1 infection are not completely recovered with cART, which may contribute to impaired cellular immune response and persistence of HIV-1.

Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma
Sumalee Obchoei, Sarah M Weakley, Sopit Wongkham, Chaisiri Wongkham, Kanlayanee Sawanyawisuth, Qizhi Yao, Changyi Chen
Molecular Cancer , 2011, DOI: 10.1186/1476-4598-10-102
Abstract: CypA expression was determined by real time RT-PCR, Western blot or immunohistochemistry. CypA silence or overexpression in CCA cells was achieved using gene delivery techniques. Cell proliferation was assessed using MTS assay or Ki-67 staining. The effect of silencing CypA on CCA tumor growth was determined in nude mice. The effect of CypA knockdown on ERK1/2 activation was assessed by Western blot.CypA was upregulated in 68% of CCA tumor tissues. Silencing CypA significantly suppressed cell proliferation in several CCA cell lines. Likewise, inhibition of CypA peptidyl-prolyl cis-trans isomerase (PPIase) activity using cyclosporin A (CsA) decreased cell proliferation. In contrast, overexpression of CypA resulted in 30% to 35% increases in proliferation of CCA cell lines. Interestingly, neither silence nor overexpression of CypA affected cell proliferation of a non-tumor human cholangiocyte cell line, MMNK1. Suppression of CypA expression attenuated ERK1/2 activity in CCA M139 cells by using both transient and stable knockdown methods. In the in vivo study, there was a 43% reduction in weight of tumors derived from CypA-silenced CCA cell lines compared with control vector CCA tumors in mice; these tumors with stable CypA silencing showed a reduced cell proliferation.CypA is upregulated in majority of CCA patients' tissues and confers a significant growth advantage in CCA cells. Suppression of CypA expression decreases proliferation of CCA cell lines in vitro and reduces tumor growth in the nude mouse model. Inhibition of CypA activity also reduces CCA cell proliferation. The ERK1/2 pathway may be involved in the CypA-mediated CCA cell proliferation. Thus, CypA may represent an important new therapeutic target for liver fluke-associated CCA.Cholangiocarcinoma (CCA) is a malignant tumor derived from bile duct epithelium. Although it is relatively rare in Europe and North America, CCA occurs at a much higher incidence in Southeast Asia; incidence and mortality rates fr
A Novel Predictive Equation for Potential Diagnosis of Cholangiocarcinoma
Ratthaphol Kraiklang, Chawalit Pairojkul, Narong Khuntikeo, Kanokwan Imtawil, Sopit Wongkham, Chaisiri Wongkham
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089337
Abstract: Cholangiocarcinoma (CCA) is the second most common-primary liver cancer. The difficulties in diagnosis limit successful treatment of CCA. At present, histological investigation is the standard diagnosis for CCA. However, there are some poor-defined tumor tissues which cannot be definitively diagnosed by general histopathology. As molecular signatures can define molecular phenotypes related to diagnosis, prognosis, or treatment outcome, and CCA is the second most common cancer found after hepatocellularcarcinoma (HCC), the aim of this study was to develop a predictive model which differentiates CCA from HCC and normal liver tissues. An in-house PCR array containing 176 putative CCA marker genes was tested with the training set tissues of 20 CCA and 10 HCC cases. The molecular signature of CCA revealed the prominent expression of genes involved in cell adhesion and cell movement, whereas HCC showed elevated expression of genes related to cell proliferation/differentiation and metabolisms. A total of 69 genes differentially expressed in CCA and HCC were optimized statistically to formulate a diagnostic equation which distinguished CCA cases from HCC cases. Finally, a four-gene diagnostic equation (CLDN4, HOXB7, TMSB4 and TTR) was formulated and then successfully validated using real-time PCR in an independent testing set of 68 CCA samples and 77 non-CCA controls. Discrimination analysis showed that a combination of these genes could be used as a diagnostic marker for CCA with better diagnostic parameters with high sensitivity and specificity than using a single gene marker or the usual serum markers (CA19-9 and CEA). This new combination marker may help physicians to identify CCA in liver tissues when the histopathology is uncertain.
Proteomic Studies of Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes
Chantragan Srisomsap,Phannee Sawangareetrakul,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Khajeelak Chiablaem,Vaharabhongsa Bhudhisawasdi,Sopit Wongkham,Jisnuson Svasti
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/437143
Abstract: Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) occur with relatively high incidence in Thailand. The secretome, proteins secreted from cancer cells, are potentially useful as biomarkers of the diseases. Proteomic analysis was performed on the secreted proteins of cholangiocarcinoma (HuCCA-1) and hepatocellular carcinoma (HCC-S102, HepG2, SK-Hep-1, and Alexander) cell lines. The secretomes of the five cancer cell lines were analyzed by SDS-PAGE combined with LC/MS/MS. Sixty-eight proteins were found to be expressed only in HuCCA-1. Examples include neutrophil gelatinase-associated lipocalin (lipocalin 2), laminin 5 beta 3, cathepsin D precursor, desmoplakin, annexin IV variant, and annexin A5. Immunoblotting was used to confirm the presence of lipocalin 2 in conditioned media and cell lysate of 5 cell lines. The results showed that lipocalin 2 was a secreted protein which is expressed only in the conditioned media of the cholangiocarcinoma cell line. Study of lipocalin 2 expression in different types of cancer and normal tissues from cholangiocarcinoma patients showed that lipocalin 2 was expressed only in the cancer tissues. We suggest that lipocalin 2 may be a potential biomarker for cholangiocarcinoma.
Proteomic Identification of Plasma Protein Tyrosine Phosphatase Alpha and Fibronectin Associated with Liver Fluke, Opisthorchis viverrini, Infection
Jarinya Khoontawad, Umawadee Laothong, Sittiruk Roytrakul, Porntip Pinlaor, Jason Mulvenna, Chaisiri Wongkham, Puangrat Yongvanit, Chawalit Pairojkul, Eimorn Mairiang, Paiboon Sithithaworn, Somchai Pinlaor
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045460
Abstract: Opisthorchiasis caused by Opisthorchis viverrini induces periductal fibrosis via host immune/inflammatory responses. Plasma protein alteration during host-parasite interaction-mediated inflammation may provide potential diagnostic and/or prognostic biomarkers. To search for target protein changes in O. viverrini-infected hamsters, a 1-D PAGE gel band was trypsin-digested and analyzed by a LC-MS/MS-based proteomics approach in the plasma profile of infected hamsters, and applied to humans. Sixty seven proteins were selected for further analysis based on at least two unique tryptic peptides with protein ID score >10 and increased expression at least two times across time points. These proteins have not been previously identified in O. viverrini-associated infection. Among those, proteins involved in structural (19%), immune response (13%), cell cycle (10%) and transcription (10%) were highly expressed. Western blots revealed an expression level of protein tyrosine phosphatase alpha (PTPα) which reached a peak at 1 month and subsequently tended to decrease. Fibronectin significantly increased at 1 month and tended to increase with time, supporting proteomic analysis. PTPα was expressed in the cytoplasm of inflammatory cells, while fibronectin was observed mainly in the cytoplasm of fibroblasts and the extracellular matrix at periductal fibrosis areas. In addition, these protein levels significantly increased in the plasma of O. viverrini-infected patients compared to healthy individuals, and significantly decreased at 2-months post-treatment, indicating their potential as disease markers. In conclusion, our results suggest that plasma PTPα and fibronectin may be associated with opisthorchiasis and the hamster model provides the basis for development of novel diagnostic markers in the future.
. W.
Nieuwe West-Indische Gids , 1951,
Creation of High Energy/Intensity Bremsstrahlung by a Multi-Target and Focusing of the Scattered Electrons by Small-Angle Backscatter at a Cone Wall and a Magnetic Field—Enhancement of the Outcome of Linear Accelerators in Radiotherapy  [PDF]
W. Ulmer
International Journal of Medical Physics,Clinical Engineering and Radiation Oncology (IJMPCERO) , 2013, DOI: 10.4236/ijmpcero.2013.24020
Abstract: The yield of bremsstrahlung (BS) from collisions of fast electrons (energy at least 6 MeV) with a Tungsten target can be significantly improved by exploitation of Tungsten wall scatter in a multi-layered target. A simplified version of a previously developed principle is also able to focus on small angle scattered electrons by a Tungsten wall. It is necessary that the thickness of each Tungsten layer does not exceed 0.04 mm—a thickness of 0.03 mm is suitable for accelerators in medical physics. Further focusing of electrons results from suitable magnetic fields with field strength between 0.5 Tesla and 1.2 Tesla (if the cone with multi-layered targets is rather narrow). Linear accelerators in radiation therapy only need to be focused by wall scatter without further magnetic fields (a standard case: 31 plates with 0.03 mm thickness and 1 mm distance between the plates). We considered three cases with importance in medical physics: A very small cone with an additional magnetic field for focusing (the field diameter at 90 cm depth: 6 cm), a medium cone with an optional magnetic field (field diameter at 90 cm depth: 13 cm) and a broad cone without a magnetic field (
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