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Search Results: 1 - 10 of 187758 matches for " W. T. Artama "
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THE EFFECT OF VIRGIN COCONUT OIL ON LYMPHOCYTE AND CD4 IN CHICKEN VACCINATED AGAINST Avian Influenza VIRUS
E. Y. W. Yuniwarti,W. Asmara,W. T. Artama,C. R. Tabbu
Journal of the Indonesian Tropical Animal Agriculture , 2012,
Abstract: This research aimed to find preventing alternative of avian influenza (AI) disease in broiler chicken by increasing body immune. Lymphocyte T would directly react to antigen presented to the cell surface by antigen presenting cell (APC). Th-CD4 interaction functioned to maintain Th-APC bond intact during specific antigen activation. Fatty acid in virgin coconut oil (VCO) was potential as immunostimulant, which therefore could increase chicken immunity through the increase of lymphocyte T and Th-CD4. This research used 40 one-day-old broiler chickens. The method applied was Completely Randomized Factorial Design in which the first factor was two levels of vaccine, namely groups of AI vaccinated and unvaccinated. The second factor was four levels of VCO namely 0, 5, 10, 15 mL/kg feed. Day Old Chick (DOC) were divided into eight treatment groups and repeated five times. Feed and water were given ad libitum for four weeks. The result showed that the number of lymphocyte and Th-CD4 in chickens given 10 mL per kg feed and vaccinated with AI was higher than that in chickens given VCO without AI vaccine.
RFLP Marker Variation of Cytocrome b Gene and Genetic Relationship among Batur, Merino and Local Sheep Breeds
Prayitno,T Hartati,R Pratiwi,WT Artama
Journal of Animal Production , 2011,
Abstract: RFLP analysis of mitochondrial DNA cytochrome b gene was conducted to determine the diversity, status and close genetic relationships in a population of Batur sheep with the Merino and local sheep breeds (Garut, Thin Tail and Fat Tail). The research used genomic DNA of 27 samples of Batur, 15 Merino, 17 Garut, 15 Thin Tails and 15 Fat Tails sheep. The PCR process used two types of 25 nucleotides primers. The PCR products were checked by using 2% agarose gel electrophoresis. The PCR DNA fragment was digested by using Hae III at 37 oC and incubated for 10 hours. Similarities and differences of cytochrome b gene RFLP bands between individual samples of one and across populations, genetic distance, and close genetic relationship, were identified. The PCR process of the cytochrome b gene metochondrial DNA of the 45 samples of sheep yielded 359 bp band types. The digestion (cutting) of the PCR products of mitochondrial DNA cytochrome b gene by using Hae III resulted in RFLP band profiles of 128 up to 231 bp polymorphisms of cytochrome b gene. Although the Hae III restriction enzyme recognized only one restriction site, however, between samples of Batur, Merino, Garut, Thin Tail, and Fat Tails, there were monomorphism and polymorphism Hae III loci.Key Words: RFLP, cytochrome b gene, genetic markers, genetic similarity, Batur shee
Genetic Relatedness Between Batur, Merino and Local Sheep Based on Random Amplyfied Polymorphism DNA Marker
Prayitno,T Hartatik,R Pratiwi,WT Artama
Journal of Animal Production , 2011,
Abstract: RAPD analysis to determine the diversity, status and genetic close relatioship Batur with Merino, Garut, Thin Tail and Fat Tail sheep genomic DNA was used in 27 Batur, 15 Merino, 17 Garut, 15 Thin Tails and Fat Tails animals. The process of RAPD-PCR used five primers of 10 nucleotides. PCR results were electrophoresed with 2% agarose gel. Identified similarities and differences between individual RAPD bands one and cross-sample of the population, genetic distance and closeness of genetic relationship. The process 89 sample sheep RAPD-PCR generated of 4189 band from 100 to 1500 bp which consisted of 91 type. Batur sheep samples produced bands at most (1666 tape) and the lowest Fat Tails (493 bands). The number monomorfism of bands most of the Batur (891 bands) and the lowest Garut (170 bands), but the polymorphism band's highest of the Batur (775 bands) and the lowest Fat Tails (262 bands). Between individuals within populations have similar genetic Merino highest and the lowest Thin Tail. Cross-population genetic similarity is highest individuals in the population Batur and Merino, while the lowest Merino and Thin Tail. The closest genetic distance was Batur with Merino population and the most apart distant Merino with Thin Tail or Merino and Fat Tails. Batur sheep population has the closest genetic relationship with the Merino and the most apart distant is with Fat Tail. (Animal Production 13(1):30-38 (2011)Key Words: RAPD, genetic markers, genetic similarity, sheep
Expression and Isolation of Recombinant Microneme 3 (MIC3) Protein of Toxoplasma gondii Local Isolate on Eschericia coli (BL21)
D Indrasanti,A Haryanto,WT Artama
Journal of Animal Production , 2011,
Abstract: Toxoplasmosis is a disease that infects all warm-blooded animals, including livestocks and humans caused by Toxoplasma gondii parasites. There are major drugs used for the therapy, though they have some effects to the patients, such as allergy, toxic and teratogenic for fetus. In addition, toxoplasmosis treatment is only effective for tachyzoites T. gondii in acute infection, while tissue cysts cannot be eradicated in chronic toxoplasmosis Tissue cysts of T. gondii contained in meat that are consumed by humans and meat-derived products may be important sources of infection for humans. Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC3) is the protein that plays an important role in the invasion process during cell infection as a mediator attachment parasite to the host cell. Recombinant MIC3 protein has been already used for the detection of toxoplasmosis and it could induce humoral and cellular immune response in experimental animals. The aim of this research was to express MIC3 recombinant protein of T. gondii from local isolate that was cloned into expression vector and transformed to E. coli BL21. In the future, recombinant protein MIC3 can be used for vaccine candidate and diagnostic tools for toxoplasmosis in animals and humans. Gene of MIC3 T. gondii local isolate (1.2 Kbp) was cloned into expression vector pET-32a(+) (5.9 Kbp) and transformed to Escherichia coli BL21. Protein from plasmid recombinant (7.1 Kbp) was expressed and performed by culturing recombinant bacteria into LB medium containing ampicillin and IPTG. Recombinant protein was isolated by sonication method and identified using SDS-PAGE. Finally, the recombinant protein was analyzed by immunoblotting using anti-ESAs polyclonal antibody. In conclusion, expression of the MIC3 gene with ~108 kDa has been successfully performed by cloning gene encoding for MIC3 protein of T. gondii local isolate that can be identified with polyclonal antibody anti-ESAs.Key Words: Toxoplasma gondii, expression, MIC3 protein
The Design of Stall-Regulated Wind Turbine Blade for a Maximum Annual Energy Output and Minimum Cost of Energy Based on a Specific Wind Statistic  [PDF]
W. Sridech, T. Chitsomboon
Journal of Power and Energy Engineering (JPEE) , 2014, DOI: 10.4236/jpee.2014.26002
Abstract:

The design of a stall-regulated wind turbine to achieve a maximum annual energy output is still a formidable task for engineers. The design could be carried out using an average wind speed together with a standard statistical distribution such as a Weibull with k = 2.0. In this study a more elaborated design will be attempted by also considering the statistical bias as a design criterion. The wind data used in this study were collected from three areas of the Lamtakong weather station in Nakhonratchasima Provice, the Khaokoh weather station in Phetchaboon and the Sirindhorn dam weather station in Ubonratchathani, Thailand. The objective is to design a best aerodynamic configurations for the blade (chord, twist and pitch) using the same airfoil as that of NREL Phase VI wind turbine. Such design is carried out at a design wind speed point. Wind turbine blades were optimized for both maximum annual energy production and minimum cost of energy using a method that take into account aerodynamic and structural considerations. The work will be carried out by the program “SuWiTStat” which was developed by the authors and based on BEM Theory (Blade Element Momentum). Another side issue is the credibility of the Weibull statistic in representing the real wind measurement. This study uses a regression analysis to determine this issue.

Use of psychotropic drugs before pregnancy and the risk for induced abortion: population-based register-data from Finland 1996-2006
Mika Gissler, Miia Artama, Annukka Ritvanen, Kristian Wahlbeck
BMC Public Health , 2010, DOI: 10.1186/1471-2458-10-383
Abstract: The Finnish Medication and Pregnancy database (N = 622 671 births and 114 518 induced abortions for other than fetal reasons) in 1996-2006 was utilised to study the use of psychotropic drugs in the three months before a pregnancy ending in a birth or an induced abortion.In total 2.1% of women with a birth and 5.1% of women with an induced abortion had used a psychotropic medicine 0-3 months before pregnancy. Psychotropic drug users terminated their pregnancies (30.9%) more often than other pregnant women (15.5%). Adjustment for background characteristics explained one third of this elevated risk, but the risk remained significantly increased among users of psychotropic medicine (OR 1.94, 95% confidence intervals 1.87-2.02). A similar risk was found for first pregnancies (30.1% vs. 18.9%; adjusted OR 1.53, 95% confidence intervals 1.42-1.65). The rate for terminating pregnancy was the highest for women using hypnotics and sedatives (35.6% for all pregnancies and 29.1% for first pregnancies), followed by antipsychotics (33.9% and 36.0%) and antidepressants (32.0% and 32.1%).The observed increased risk for induced abortion among women with psychotropic medication highlighs the importance to acknowledge the mental health needs of women seeking an induced abortion. Further studies are needed to establish the impact of pre-existing differences in mental health on mental health outcomes of induced abortions compared to outcomes of pregnancies ending in a birth.The connection of induced abortion and subsequent mental health problems is controversial. Studies from countries with free or relatively liberal abortion legislation have suggested that women experiencing an induced abortion have a higher risk for depression [1-5], anxiety [2,5-10], suicide [11,12], suicidal behaviour [2], substance use disorders [2], and any mental health problem requiring outpatient treatment [13] after the procedure. In most of these studies, the available confounding factors did not explain the
Peran Kondisi Pemangku Kepentingan Dalam Keberhasilan Proyek
Herry Pintardi Chandra,Indarto .,I Putu Artama Wiguna,Peter Kaming
Jurnal Manajemen dan Kewirausahaan , 2011,
Abstract: There is a natural tendency for a stakeholder groups to try to influence their condition in project success. This presents a challenge for construction project player in analyzing and managing these various concerns and needs in the implementation of project. The aim of the research presented here is to show how well the factors affecting the stakeholder condition in project success. These uncertainty condition factors that come from stakeholder can be identified into three parts: stakeholder impact, stakeholder engagement, and stakeholder psychological empowerment. It was hypothesized that project success is influenced by stakeholder impact, stakeholder engagement, and stakeholder psychological empowerment. The data obtained from a questionnaire survey administered to 204 respondents as construction project players were analyzed by using Structural Equation Modeling (SEM). The results of this research verify the hypothesis suggested. Stakeholder condition that can be defined as stakeholder impact, stakeholder engagement, and stakeholder psychological empowerment has a positive significance related to project success.
Genetic Diversity of k-Casein Gene of Friesian-Holstein Dairy Cattle using Restriction Fragment Length Polymorphism Technique
Rini Widayanti,Wayan Tunas Artama,Slamet Subagyo,Djoko Winarso
Journal of Animal Production , 2008,
Abstract: The objective of the research was to evaluate the genetic varieties of k -Casein gene of Holstein Friesian (HF) in Koperasi Unit Desa (KUD) Karangploso, Dau, Ngantang and Pujon, Malang, East Java by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis (PCR-RFLP) using Pst-l. The research used Holstein Friesian dairy cows raised by farmers of KUD Karangploso, Dau, Ngantang and Pujon, Malang, East Java. The blood samples were collected from 29 cows. The research activities were carried out by collecting cow’s blood, DNA extraction, and DNA amplification with PCR. PCR products were digested with Pst-l enzyme restrictions, then allele genes of k- Casein were identified. The frequency of allele and genotype of k-Casein gene were calculated by Hardy-Weinberg. The results showed that cows raised by farmers of KUD Karangploso, Dau, Ngantang and Pujon, Malang-East Java had two alleles k- Casein polymorphism, i.e. allele A (0,74) and B (0,26); therefore, two genotypes existed including AA (0,55) and AB (0,38). It can be concluded that genetic diversity of k-Casein gene existed in Holstein-Friesian dairy cows raised by farmers of KUD Ngantang, Pujon, Dau and Karangploso, Malang, East Java. (Animal Production 10(1): 1-4 (2008) Key Words: Pst-1, k-casein gene, PCR-RFLP
Thermal and structural studies of poly (vinyl alcohol) and hydroxypropyl cellulose blends  [PDF]
Osiris W. Guirguis, Manal T. H. Moselhey
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.41009
Abstract: Polymers and polymeric composites have steadily reflected their importance in our daily life. Blending poly(vinyl alcohol) (PVA) with a potentially useful natural biopolymers such as hydroxypropyl cellulose (HPC) seems to be an interesting way of preparing a polymeric blends. In the present work, blends of PVA/HPC of compositions (100/0, 90/10, 75/25, 50/50, 25/75, and 0/100 wt/wt%) were prepared to be used as bioequivalent materials. Thermal analyses [differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)], and X-ray diffraction (XRD) were employed to characterize and reveal the miscibility map and the structural properties of such blend system. The obtained results of the thermal analyses showed variations in the glass transition temperature (Tg) indicating the miscibility of the blend systems. Moreover, the changes in the melting temperature (Tm), shape and area were attributed to the different degrees of crystallinity and the existence of polymer-polymer interactions between PVA and HPC molecules. The X-ray diffraction (XRD) analysis showed broadening and sharpening of peaks at different HPC concentrations with PVA. This indicated changes in the crystallinity/amorphosity ratio, and also suggested that the miscibility between the amorphous components of homo-polymers PVA and HPC is possible. The results showed that HPC doped in PVA film can improve the thermal stability of the film under investigation, leading to interesting technological applications.
Light Emitting Diodes as a Rapid Visual Display for Use in Psychological Experiments  [PDF]
Clementine Thurgood, T. W. Allan Whitfield
Optics and Photonics Journal (OPJ) , 2013, DOI: 10.4236/opj.2013.31015
Abstract:

In this paper we show how light emitting diodes (LEDs) can be used in conjunction with existing display technologies as a means for achieving ultra-rapid visual stimulus exposure durations. We review existing rapid visual display methods, and show how our apparatus overcomes the limitations inherent with each technique. Our apparatus, the LED tachistoscope, takes advantage of the fast-switching times and high-brightness capabilities of LEDs in order to present stimuli at previously unachievable durations as rapid as 1 ms. The rapid exposure durations are achieved by external LED backlight illumination of images on a liquid crystal display (LCD) after the components of the LCD have stabilized. This ensures that stimulus onset and offset are discrete. Furthermore, the fast-switching of the LEDs enables stimuli to be revealed for very rapid durations. The paper also describes studies in which the LED tachistoscope has already been applied, and offers suggestions for other possible applications. Interestingly, in our studies we show that the human visual system is very adept at extracting information with only very minimal stimulus exposure durations. Such studies have not been possible with existing display equipment. The LED tachistoscope opens up avenues for a variety of psychological and physiological experiments and provides a means for revealing the limits of human visual perception.

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