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Search Results: 1 - 10 of 2047 matches for " Vishwa Mohan Katoch "
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2D-QSAR model development and analysis on variant groups of anti -tuberculosis drugs
Neeraja Dwivedi*,Bhartendu Nath Mishra,Vishwa Mohan Katoch
Bioinformation , 2011,
Abstract: A quantitative structure activity relationship study was performed on different groups of anti-tuberculosis drug compound for establishing quantitative relationship between biological activity and their physicochemical /structural properties. In recent years, a large number of herbal drugs are promoted in treatment of tuberculosis especially due to the emergence of MDR (multi drug resistance) and XDR (extensive drug resistance) tuberculosis. Multidrug-resistant TB (MDR-TB) is resistant to front-line drugs (isoniazid and rifampicin, the most powerful anti-TB drugs) and extensively drug-resistant TB (XDR-TB) is resistant to front-line and second-line drugs. The possibility of drug resistance TB increases when patient does not take prescribed drugs for defined time period. Natural products (secondary metabolites) isolated from the variety of sources including terrestrial and marine plants and animals, and microorganisms, have been recognized as having antituberculosis action and have recently been tested preclinically for their growth inhibitory activity towards Mycobacterium tuberculosis or related organisms. A quantitative structure activity relationship (QSAR) studies were performed to explore the antituberculosis compound from the derivatives of natural products . Theoretical results are in accord with the in vitro experimental data with reported growth inhibitory activity towards Mycobacterium tuberculosis or related organisms. Antitubercular activity was predicted through QSAR model, developed by forward feed multiple linear regression method with leave-one-out approach. Relationship correlating measure of QSAR model was 74% (R2 = 0.74) and predictive accuracy was 72% (RCV2 = 0.72). QSAR studies indicate that dipole energy and heat of formation correlate well with anti-tubercular activity. These results could offer useful references for understanding mechanisms and directing the molecular design of new lead compounds with improved anti-tubercular activity. The generated QSAR model revealed the importance of structural, thermodynamic and electro topological parameters. The quantitative structure activity relationship provides important structural insight in designing of potent antitubercular agent.
Detection of Mycobacterium leprae DNA for 36kDa protein in urine from leprosy patients: a preliminary report
Parkash, Om;Singh, Hari Bhan;Rai, Subha;Pandey, Archna;Katoch, Vishwa Mohan;Girdhar, Bhawneshwar Kumar;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000500008
Abstract: we have searched for mycobacterium leprae dna for 36kda protein in urine using a m. leprae specific pcr technique. a limited number of 16 patients (of which 11 belonged to lepromatous leprosy and five to tuberculoid leprosy) and eight healthy individuals were included for the present study. the number of urine samples positive by pcr were 36.4% (4/11) in lepromatous patients and 40% (2/5) in tuberculoid patients. none of the samples from healthy individuals was positive. to our knowledge, the results indicate, for the first time, the presence of m. leprae dna in urine from leprosy patients. another important finding obtained out of the study is that amongst treated patients 66.6% (4/6) were positive whereas amongst untreated only 20% (2/10) were positive. from the present indicative data it appears that treatment improves the pcr results with urine as a sample. thus, the approach could prove to be useful for monitoring the treatment response of individual patients and needs to be further evaluated with a large number of patients.
Computational identification of composite regulatory sites in 16s-rRNA gene promoters of Mycobacterium species
Neeraja Dwivedi1, , and , *,Surya Prakash Dwivedi,Ajay kumar,Vishwa Mohan Katoch
Bioinformation , 2008,
Abstract: The availability of completely sequenced genomes allow the use of computational techniques to investigate cis-acting sequences controlling transcription regulation associated with groups of functionally related genes. Theoretical analysis was performed to assign functions to regulatory systems. The identification of such sites is relevant for locating a promoter at the 5' boundary of a gene. They also allow the prediction of specific gene-expression pattern and response to disturbances in a known signaling pathway. Here, we describe the identification of composite transcription factor (TF) binding sites over promoter regions in16s-rRNA gene for mycobacterium species strains ICC47, ICC67, ICC43 and CMVL700. It is established that the ribosomal gene comprises of sequences that are conserved during evolution and interspersed with divergent regions. Computational identification of known TF-binding sites was performed using TFSITESCAN tool and ooTFD database. The ICC67, ICC47, ICC43 and CMYL700 strains showed 12, 13, 9 and 15 known TF binding sites, respectively. Comparison between strains suggests 9 known TF predicted binding sites to be conserved among them. These data provide basis for the understanding of promoter regulation in 16s-rRNA.
Nocardial mycetoma: Diverse clinical presentations
Sharma Nand,Mahajan Vikram,Agarwal Santwana,Katoch Vishwa
Indian Journal of Dermatology, Venereology and Leprology , 2008,
Abstract: Nocardia spp are gram-positive, aerobic, acid-fast bacteria which exist as saprophytes in nature. Invasive disseminated infections are particularly common in immunocompromised or debilitated hosts. Superficial infections with Nocardia spp occur as a result of local trauma and contamination of the wound. Clinically, it presents as acute infection (abscesses or cellulitis), mycetoma, or sporotrichoid infection. Differential diagnosis includes eumycetoma, chromomycosis, blastomycosis, coccidioidomycosis, sporotrichosis, tuberculosis, botryomycosis, syphilis, yaws, and neoplasia. Its diagnosis is confirmed by demonstrating the causative organism in exudates (as granules), tissue specimens, or cultures. Early diagnosis will obviate need for drastic surgical measures as early institution of chemotherapy is effective in most patients. However, its diagnosis is often delayed due to diverse clinical presentations and for want of clinical suspicion, particularly in non-endemic areas. This paper presents 4 clinical forms of this not so uncommon disease, emphasizing the importance of high index of clinical suspicion, especially in non-endemic regions; and the significance of repeated examination of exudates for Nocardia granules for an early diagnosis.
Application of Web 2.0 Tools in Medical Librarianship to Support Medicine 2.0
Vahideh Zarea Gavgani,V. Vishwa Mohan
Webology , 2008,
Abstract: It is almost a decade that social networking technology along with its tools such as blogs, wikis, slidesharing/videosharing and photosharing softwares, podcasts, RSS feeds, mashups, folksonomies, and bookmarks has developed its influence on all human fields of study/activity. It is obvious that these tools are increasingly growing, in different languages, regions and fields, due to social dynamic and liberal characteristic of Web 2.0 technologies. Medical sciences and library science also are not exception to this influence. Consequently, library 2.0 and newly coined concepts of Medicine 2.0, and Health 2.0 have become the buzzwords in the Internet culture. In spite of proliferation of such social tools listed above, there is no aggregation and harmony for the utilization of the potential of these technologies in specific subject areas and the sources of information on the Web is almost proliferating and uncontrolled. This gives rise to the problem of webliographic control due to which the information seekers find difficulties in information retrieval. The paper presents application of Web 2.0 in medical libraries to support Medicine 2.0 emphasizing the above-mentioned problems. Considering the nature of an original article the experience of the authors, as a medical librarian and a faculty member in Library and Information Science, through observation of the needs, problems and prospects, played an important role in forming the idea and presentation. The study also used secondary data collected from related literatures. Standardization and webliographic control would solve the problem. In addition, governmental support and creating awareness at the management level in the organizations is also crucial.
Assessment of the N-PCR Assay in Diagnosis of Pleural Tuberculosis: Detection of M.tuberculosis in Pleural Fluid and Sputum Collected in Tandem
Parameet Kumar,Manas K. Sen,Devendra S. Chauhan,Vishwa M. Katoch,Sarman Singh,Hanumanthappa K. Prasad
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0010220
Abstract: The nonspecific clinical presentation and paucibacillary nature of tuberculous pleuritis remains a challenge for diagnosis. Diagnosis of tuberculous pleural effusion depends on the demonstration of the presence of tubercle bacilli in the sputum, pleural fluid, or pleural biopsy specimen, or demonstration of granuloma in pleura by histological examination. We examined the clinical utility of the diagnosis of pleural tuberculosis using the in house N-PCR assay, AFB smear microscopy and culture. Besides pleural fluid the inclusion of sputum in the efficacy of diagnosis of pleural tuberculosis was scrutinized.
Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation
Sharmistha Banerjee, Ashok Nandyala, RaviPrasad Podili, Vishwa Katoch, Seyed E Hasnain
BMC Biochemistry , 2005, DOI: 10.1186/1471-2091-6-20
Abstract: Functionally, both M.tb ICD-1 and ICD-2 proteins are dimers. Zn+2 can act as a cofactor for ICD-1 apart from Mg+2, but not for ICD-2. ICD-1 has higher affinity for metal substrate complex (Km (isocitrate) with Mg++:10 μM ± 5) than ICD-2 (Km (isocitrate) with Mg++:20 μM ± 1). ICD-1 is active across a wider pH range than ICD-2, retaining 33–35% activity in an acidic pH upto 5.5. Difference in thermal behaviour is also observed with ICD-2 being active across wider temperature range (20°C to 40°C) than ICD-1 (optimum temperature 40°C). The isozymes are NADP+ dependent with distinct phylogenetic affiliations; unlike M.tb ICD-2 that groups with bacterial ICDs, M.tb ICD-1 exhibits a closer lineage to eukaryotic NADP+ dependent ICDs.The data provide experimental evidence to show that the two open reading frames, Rv3339c (ICD-1) and Rv0066c (ICD-2), annotated as probable ICDs are functional TCA cycle enzymes with identical enzymatic function but different physio-chemical and kinetic properties. The differences in biochemical and kinetic properties suggest the possibility of differential expression of the two ICDs during different stages of growth, despite having identical metabolic function.The central metabolic pathways in bacteria, especially in E.coli, have been extensively studied to understand the physiology of the organisms under altered carbon sources [1]. One of the key regulatory enzymes in the universal tri-carboxylic acid energy cycle is the isocitrate dehydrodenase that allosterically regulates the conversion of oxidative decarboxylation of D-isocitrate to α-ketoglutarate and CO2 in presence of a cofactor [2]. This rate-limiting step is the first NADPH yielding reaction of the TCA cycle [2]. Isocitrate dehydrogenase belongs to a family of enzymes that exhibits diversity with regard to amino acid composition, cofactor specificity, metal ion requirement and oligomeric state. NADP-linked ICDs have been purified and studied from a variety of eukaryotes and prokaryote
Proteomic analysis of streptomycin resistant and sensitive clinical isolates of Mycobacterium tuberculosis
Prashant Sharma, Bhavnesh Kumar, Yash Gupta, Neelja Singhal, Vishwa Katoch, Krishnamurthy Venkatesan, Deepa Bisht
Proteome Science , 2010, DOI: 10.1186/1477-5956-8-59
Abstract: Two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was employed for analyzing the protein profiles. Homology and in silico characterization for identified proteins was assessed using BLAST, InterProScan and KEGG database searches. Computational studies on the possible interactions between SM and identified proteins were carried out by a battery of online servers and softwares, namely, CLUSTALW (KEGG), I-TASSER, VMD, PatchDock and FireDock. On comparing 2DE patterns, nine proteins were found consistently overexpressed in SM resistant isolates and were identified as Rv0350, Rv0440, Rv1240, Rv3075c, Rv2971, Rv3028c, Rv2145c, Rv2031c and Rv0569. In silico docking analysis showed significant interactions of SM with essential (Rv0350, Rv0440 and Rv2971) and non essential (Rv1240, Rv3075c and Rv2031c) genes.The computational results suggest high protein binding affinity of SM and suggested many possible interactions between identified proteins and the drug. Bioinformatic analysis proves attributive for analysis of diversity of proteins identified by whole proteome analysis. In-depth study of the these proteins will give an insight into probable sites of drug action other than established primary sites and hence may help in search of novel chemotherapeutic agents at these new sites as inhibitors.Tuberculosis is one of the most challenging infectious diseases. Globally, 9.2 million new cases and 1.7 million deaths occur due to this disease [1]. Its impact on public health is further aggravated by co-infection with human immunodeficiency virus, emergence of multi-drug resistant strains and reactivation of the dormant bacteria. Attempt for primary prevention using Bacillus Calmette Guerin (BCG) and other integral vaccines have generally been disappointing though some subunit vaccines are under trial [2]. The excessive emergence of drug resistant tuberculosis has stimulated interest in the under
The 5th International Evidence Based Library and Information Practice Conference (EBLIP5): Conference Report and Reflections
Lotta Haglund,Diana Wakimoto,Laval Hunsucker,V. Vishwa Mohan
Evidence Based Library and Information Practice , 2009,
Investigation of Probability Generating Function in an Interdependent M/M/1:(∞; GD) Queueing Model with Controllable Arrival Rates Using Rouche’s Theorem  [PDF]
Vishwa Nath Maurya
Open Journal of Optimization (OJOp) , 2012, DOI: 10.4236/ojop.2012.12006
Abstract: Present paper deals a M/M/1:(∞; GD) queueing model with interdependent controllable arrival and service rates where- in customers arrive in the system according to poisson distribution with two different arrivals rates-slower and faster as per controllable arrival policy. Keeping in view the general trend of interdependent arrival and service processes, it is presumed that random variables of arrival and service processes follow a bivariate poisson distribution and the server provides his services under general discipline of service rule in an infinitely large waiting space. In this paper, our central attention is to explore the probability generating functions using Rouche’s theorem in both cases of slower and faster arrival rates of the queueing model taken into consideration; which may be helpful for mathematicians and researchers for establishing significant performance measures of the model. Moreover, for the purpose of high-lighting the application aspect of our investigated result, very recently Maurya [1] has derived successfully the expected busy periods of the server in both cases of slower and faster arrival rates, which have also been presented by the end of this paper.


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