oalib

OALib Journal期刊

ISSN: 2333-9721

费用:99美元

投稿

匹配条件: “Vidyadhara Suryadevara” ,找到相关结果约18条。
列表显示的所有文章,均可免费获取
第1页/共18条
每页显示
Influence of electrolytes on the controlled release of verapamil hydrochloride from HPMC K15M matrix tablets
Vidyadhara Suryadevara,Babu Janga,krishna Talamanchi
Asian Journal of Pharmaceutics , 2011,
Abstract: Verapamil hydrochloride was formulated as oral-controlled release matrix tablets using hydrophilic polymer such as hydroxypropyl methylcellulose K 15 M (HPMC 15 M) along with electrolytes. In this work a new attempt was made for in situ interactions between drug and electrolytes were devised to control the release of highly water soluble drugs from oral hydrophilic monolithic systems. Electrolytes such as aluminum hydroxide and sodium carbonate were used at different concentrations in various formulations, while drug and polymer concentrations were maintained constantly at 1:2 ratios in all the formulations. These electrolytes were used to monitor matrix swelling and gel properties. Electrolytes at higher concentrations exhibited greater inhibition in drug release from the matrix and low concentrations were accounted for controlled release of the drug. The results indicated that the drug released at a controlled rate were due to differential swelling rate and matrix stiffening, and provides a uniform gel layer. These findings indicated that the swelling and gel formation in the presence of ionizable species within the hydrophilic matrices provide an attractive alternative for controlled drug delivery from a simple monolithic system. Accelerated stability studies were carried out as per ICH guidelines for some selected formulations, which indicated that these formulations were stable at accelerated storage conditions.
Smartphone Based Fall Detection and Logic Testing Application Using Android SDK  [PDF]
Vinay Kumar Suryadevara, Maher Rizkalla
Journal of Biomedical Science and Engineering (JBiSE) , 2015, DOI: 10.4236/jbise.2015.89057
Abstract: Smart systems aimed at detecting the fall of a person have increased significantly due to recent technological advances and availability of modular electronics. This work presents the use of embedded accelerometer and gyroscope in mobile phones to accurately detect and classify the type of fall a person is experiencing before suffering an impact. Early classification of fall type helps in optimizing the algorithm of the fall detection. User acceptance, feasibility and the limitations in the accuracy of the existing devices have also been considered in this study. High efficiency and low power approaches were emphasized with wireless capability that enhanced the system performance for variety of applications. There is a need of reducing the time for analyzing the smart algorithms designed. It is also emphasized that this application will be a good platform that can be used to test various algorithms and multiple sensors at a time with ease and obtain data analysis in a short period.
Ipsilateral posterior dislocations of the elbow and superior radio-ulnar joint associated with a comminuted radial shaft fracture - A case report
Vidyadhara S,Rao Sharath
Indian Journal of Orthopaedics , 2006,
Abstract:
Development and Validation of LC-MS/MS Method for the Quantification of Chiral Separated R-Bicalutamide in Human Plasma  [PDF]
N. T. Ramarao, S. Vidyadhara, R. L. C. Sasidhar, B. Deepti, R. Surendra Yadav
American Journal of Analytical Chemistry (AJAC) , 2013, DOI: 10.4236/ajac.2013.42009
Abstract:

A simple, rapid, specific and precise liquid chromatography—tandem mass spectrophotometric (LC-MS/MS) method was developed and validated for the quantification of chiral separated R-bicalutamide from S-bicalutamide, in human plasma. Topiramate (TPM) was used as internal standard, added to plasma sample prior to extraction using t-butyl methyl ether (TBME). Chromatographic separation was achieved on CHIRALPAK AD-RH column (150 mm×4.6 mm, 5 μm) with acteonitrile: 0.1% formic acid Buffer (50:50 v/v) as an isocratic mobile phase with a flow rate of 1.0 mL·min-1. Quantitation was performed by transition of 429.0 → 255.0 (m/z) for R-bicalutamide and 338.1 → 77.8 (m/z) for topiramate. The lower limit of quantitation was 20 ng·mL-1with a 100 μL plasma sample. The concentrations of eight working standards showed linearity between 20 to 3200 ng·mL-1(r2≥ 0.9990). Chromatographic separation was achieved within 6 min, compared to the 15 min of previous methods. The average extraction recoveries of 3 quality control concentrations were 98.56% for R-bicalutamide and 92.42% for topiramate. The coefficient of variation was ≤15% for intra- and inter-batch assays. Therefore a rapid, specific and sensitive LC-MS/MS method for the quantification of R-bicalutamide in human plasma was developed and validated can be used in the bioequivalence study of this drug.

Computer Simulation/Practical Models for Human Thyroid Thermographic Imaging  [PDF]
James Rizkalla, William Tilbury, Ahdy Helmy, Vinay Kumar Suryadevara, Maher Rizkalla, Michael M. Holdmann
Journal of Biomedical Science and Engineering (JBiSE) , 2015, DOI: 10.4236/jbise.2015.84024
Abstract: We have demonstrated a successful computer model utilizing ANSIS software that is verified with a practical model using Infrared (IR) sensors. The simulation model incorporates the three heat transfer coefficients: conduction, convection, and radiation. While the conduction component was a major contributor to the simulation model, the other two coefficients have added to the accuracy and precision of the model. Convection heat allows for the influence of blood flow within the study, while the radiation aspect, sensed through IR sensors, links the practical model of the study. This study also compares simulation data with the applied model generated from IR probe sensors. These sensors formed an IR scanner that moved via servo mechanical system, tracking the temperature distribution within and around the thyroid gland. These data were analyzed and processed to produce a thermal image of the thyroid gland. The acquired data were then compared with an Iodine uptake scan for the same patients.
Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits
Suryadevara Rao, Randy D Dinkins, Arthur G Hunt
BMC Cell Biology , 2009, DOI: 10.1186/1471-2121-10-51
Abstract: It was found that AtCPSF30 by itself localizes, not to the nucleus, but to the cytoplasm. AtCPSF30 could be found in the nucleus when co-expressed with AtCPSF160 or AtCPSF73(I), one of the two Arabidopsis orthologs of CPSF73. This re-directing of AtCPSF30 indicates that AtCPSF30 is retained in the nucleus via interactions with either or both of these other CPSF subunits. Co-expression of AtCSPF30 with AtCPSF100 altered the location, not of AtCPSF30, but rather of AtCPSF100, with these proteins residing in the cytoplasm. Deletion of plant-specific N- or C-terminal domains of AtCPSF30 abolished various of the interactions between AtCPSF30 and other CPSF subunits, suggesting that the plant CPSF complex assembles via novel protein-protein interactions.These results suggest that the nuclear CPSF complex in plants is a dynamic one, and that the interactions between AtCPSF30 and other CPSF subunits are different from those existing in other eukaryotes.Messenger RNA polyadenylation is mediated by a multifactor complex in eukaryotes. In mammals, this complex consists of the Cleavage Stimulatory Factor (CstF), the Cleavage and Polyadenylation Specificity Factor (CPSF), two Cleavage Factors (CFIm and CFIIm) and poly(A) polymerase [1-3]. CPSF binds to the AAUAAA sequence via its 160 kD subunit [4], and CstF recognizes the downstream element via its 64 kD subunit [5-7]. Poly(A) tail formation is further controlled by a nuclear poly (A)-binding protein [8]. The polyadenylation of pre-mRNAs occurs in the nucleus, and is coupled to the transcription process at many different steps.Among the more interesting of the subunits of the polyadenylation complex is the 30 kD subunit of CPSF (CPSF30). CPSF30 proteins possess tandem arrays of 3–5 CCCH zinc finger motifs [9-14] that are involved in a number of functions of the proteins. CPSF30 from Drosophila melanogaster [11], mammals [10], yeast [9,10,15], and Arabidopsis [13,14] bind RNA. Moreover, the Arabidopsis and Drosophila CPSF30 prot
Genetic Association of Interferon Gamma Induced Protein-10 (IP-10), CXCL-10 Gene Polymorphisms with TB Pleurisy Susceptibility in South Indian Population  [PDF]
Ghousunnissa Sheikh, Venkata Sanjeev Kumar Neela, Satya Sudheer Pydi, Naveen Chandra Suryadevara, Ramulu Gaddam, Suman Latha Gaddam, Sai Kumar Auzumeedi, Vijaya Lakshmi Valluri
Open Journal of Immunology (OJI) , 2015, DOI: 10.4236/oji.2015.52008
Abstract: CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (1447A > G and 135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the 135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and 135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at 135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.
Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agent
Suryadevara S Rao, Lewamy Mamadou, Matt McConnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand
BMC Biotechnology , 2009, DOI: 10.1186/1472-6750-9-94
Abstract: When tested against different alternate selection agents our studies show that 0.16 μg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed? and Synchrony? both at 150 μg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, lysine+threonine (LT) were poor selection agents. The use of AEC may be preferable since it is a natural compound. Unlike the plant enzyme, dihydrodipicolinate synthase (DHPS) from E. coli is not feed-back inhibited by physiological concentrations of lysine. The dapA gene which codes for E. coli DHPS was expressed in soybean somatic embryos under the control of the CaMV 35S promoter. Following introduction of the construct into embryogenic tissue of soybean, transgenic events were recovered by incubating the tissue in liquid medium containing AEC at a concentration of 5 mM. Only transgenic soybeans were able to grow at this concentration of AEC; no escapes were observed.Genetically engineered soybeans expressing a lysine insensitive DHPS gene can be selected with the non-antibiotic selection agent AEC. We also report here the inhibitory effects of glufosinate, (isopropylamine-glyphosate) (Roundup?), AEC and the ALS inhibitors Exceed? and Synchrony? against different tissues of soybeanA prerequisite for most current plant genetic engineering procedures is the ability to produce transgenic plants. The process of producing transgenic plants often requires effective means for identifying and selecting transgenic cells and tissues. An important method of soybean regeneration is somatic embryogenesis. Through somatic embryogenesis, genetic engineering of soybean has proved to be a powerful technique for improving seed compositions including the oil for enhanced edible and industrial purposes [1-5]. Somatic embryogenesis (SE) is the process whereby embryos develop from either microspores or somatic tissues. In contrast to the co
Unusual towering elevation of troponin I after ST-elevation myocardial infarction and intensive monitoring with echocardiography post-percutaneous coronary intervention: a case report
Fahad Javed, Shahzeb A Khan, Emad F Aziz, Taimur Abbasi, Ramya Suryadevara, Eyal Herzog
Journal of Medical Case Reports , 2010, DOI: 10.1186/1752-1947-4-137
Abstract: A 53-year-old African American man with an unknown medical history presented to the emergency room of our hospital with chest pain associated with diaphoresis and altered mental status. He required emergency intubation due to acute respiratory failure and circulatory collapse within 10 minutes of his arrival. He was started on heparin and eptifibatide (Integrilin) drips but he was taken immediately for cardiac catheterization, which showed a total occlusion of his proximal left anterior descending, diffuse left circumflex disease and severe left ventricular dysfunction with segmental wall motion abnormality. He remained hypotensive throughout the procedure and an intra-aortic balloon pump was inserted for circulatory support. His urinary toxicology examination result was positive for cocaine metabolites. Serial echocardiograms showed an akinetic apex, a severely hypokinetic septum, and severe systolic dysfunction of his left ventricle. Our patient stayed at the Coronary Care Unit for a total of 15 days before he was finally discharged.Studies demonstrate that an increase of 1 ng/ml in the cardiac troponin I level is associated with a significant increase in the risk ratio for death. The elevation of troponin I to 515 ng/ml in our patient is an unusual robust presentation which may reflect a composite of myocyte necrosis and reperfusion but without short-term mortality. Nevertheless, prolonged close monitoring is required for better outcome. We also emphasize the need for the troponin assays to be standardized and have universal cutoffs for comparisons across available data.ST elevation myocardial infarction with elevated cardiac enzymes is a common scenario in emergency rooms. Nowadays, it has become more evident in patients with cocaine abuse. The elevation of troponins directly corresponds to the extent of myocardial injury. We present a case of a robust rise in cardiac biomarkers that correspond to extensive damage to the myocardium but did not spell doom for our
The clinical behavior of mixed ductal/lobular carcinoma of the breast: a clinicopathologic analysis
Aparna Suryadevara, Lakshmi P Paruchuri, Nassim Banisaeed, Gary Dunnington, Krishna A Rao
World Journal of Surgical Oncology , 2010, DOI: 10.1186/1477-7819-8-51
Abstract: In this paper, we present a clinicopathologic analysis of patients at our institution with this entity and compare them to age-matched controls with purely invasive ductal carcinoma (IDC) and historical data from patients with purely lobular carcinoma and also stain-available tumor specimens for E-cadherin. We have obtained 100 cases of ductal and 50 cases of mixed ductal/lobular breast carcinoma.Clinically, the behavior of mixed ductal/lobular tumors seemed to demonstrate some important differences from their ductal counterparts, particularly a lower rate of metastatic spread but with a much higher rate of second primary breast cancers.Our data suggests that mixed ductal/lobular carcinomas are a distinct clinicopathologic entity incorporating some features of both lobular and ductal carcinomas and representing a pleomorphic variant of IDC.Infiltrating ductal carcinoma is the most common type of invasive breast cancer, accounting for 65% to 80% of invasive breast lesions[1,2]. Its characteristics have been well described, including average age of onset, its rate of hormone receptor and erbB2 positivity, frequency of nodal involvement, rates of metastatic spread, and overall survival[3]. Historically, invasive lobular carcinomas (ILC) represented the second most common subtype of mammary neoplasia, accounting for about 5% to 10% of the disease[4]. The clinical behavior of ILC has been known to be different since its recognition as a distinct clinicopathologic entity[5]. Lobular carcinomas that are more frequently hormone-receptor positive[6] display a higher incidence of synchronous, contralateral primary tumors[7], more frequently present with multicentric disease[8], and metastasize to distinct sites such as the meninges, serosa, and retroperitoneum[9]. Given the difference in behavior between the two subtypes and the unique behavior of the ILC, the initial diagnostic workup has often involved the use of bilateral breast MRI to assess the state of the contralateral
第1页/共18条
每页显示


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.