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Search Results: 1 - 10 of 139257 matches for " Verena K. Capellini "
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The Effect of Extracellular pH Changes on Intracellular pH and Nitric Oxide Concentration in Endothelial and Smooth Muscle Cells from Rat Aorta
Verena K. Capellini, Carolina B. A. Restini, Lusiane M. Bendhack, Paulo R. B. Evora, Andréa C. Celotto
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0062887
Abstract: Aims It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of pH changes on vascular response. In this study, we investigated the effects of extracellular pH (pHo) changes on intracellular pH (pHi) and intracellular nitric oxide concentration ([NO]i) in freshly isolated endothelial cells and cross sections from rat aorta. Main Methods The HCl was used to reduce the pHo from 7.4 to 7.0 and from 7.4 to 6.5; the NaOH was used to increase the pHo from 7.4 to 8.0 and from 7.4 to 8.5. The fluorescent dyes 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (SNARF-1) and diaminofluorescein-FM diacetate (DAF-FM DA) were employed to measure the pHi and [NO]i, respectively. The fluorescence intensity was measured in freshly isolated endothelial cells by flow cytometry and in freshly obtained aorta cross sections by confocal microscopy. Key Findings The endothelial and vascular smooth muscle pHi was increased at pHo 8.5. The extracellular acidification did not change the endothelial pHi, but the smooth muscle pHi was reduced at pHo 7.0. At pHo 8.5 and pHo 6.5, the endothelial [NO]i was increased. Both extracellular alkalinization and acidification increased the vascular smooth muscle [NO]i. Significance Not all changes in pHo did result in pHi changes, but disruption of acid-base balance in both directions induced NO synthesis in the endothelium and/or vascular smooth muscle.
Vascular relaxation of canine visceral arteries after ischemia by means of supraceliac aortic cross-clamping followed by reperfusion
José G Ciscato, Verena K Capellini, Andrea C Celotto, Caroline F Baldo, Edwaldo E Joviliano, Paulo RB Evora, Marcelo B Dalio, Carlos E Piccinato
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine , 2010, DOI: 10.1186/1757-7241-18-41
Abstract: Male mongrel dogs (n = 27) were randomly allocated in one of the three groups: sham (no clamping, n = 9), ischemia (supraceliac aortic cross-clamping for 60 minutes, n = 9), and I/R (60 minutes of ischemia followed by reperfusion for 30 minutes, n = 9). Relaxation of visceral arteries (celiac trunk, renal and superior mesenteric arteries) was studied in organ chambers. MDA and NOx concentrations were determined using a commercially available kit and an ozone-based chemiluminescence assay, respectively.Both acetylcholine and calcium ionophore caused relaxation in endothelium-intact rings and no statistical differences were observed among the three groups. Sodium nitroprusside promoted relaxation in endothelium-denuded rings, and there were no inter-group statistical differences. Both plasma and renal concentrations of MDA and NOx showed no significant difference among the groups.Supraceliac aortic cross-clamping for 60 minutes alone and followed by 30 minutes of reperfusion did not impair relaxation of canine visceral arteries nor evoke biochemical alterations in plasma or renal tissue.Traumatic injuries still constitute one of the leading causes of death in all age groups [1]. Intrathoracic or subdiaphragmatic haemorrhage due to trauma is a life-threatening injury and the management of the massive haemorrhage is a great challenge in acute trauma care that often requires emergency surgical repair [1-3]. The supraceliac aortic cross-clamping can be an option to save critical patients with hipovolemic shock due to abdominal trauma [4,5]. However, this maneuver is associated with various complications including myocardial dysfunction, pulmonary disease, renal insufficiency/failure, liver failure, ischemic enterocolitis, coagulopathy and paraparesis/paraplegia [4,6]. Multiple organ failure results from the ischemia and reperfusion (I/R) injury [6], an universal phenomenon that has been extensively studied.The I/R injury is characterized by an increase in circulating medi
The protective effect of cilostazol on isolated rabbit femoral arteries under conditions of ischemia and reperfusion: the role of the nitric oxide pathway
Santos, Mariana R.G.A.;Celotto, Andréa C.;Capellini, Verena K.;Evora, Paulo R. B.;Piccinato, Carlos E.;Joviliano, Edwaldo E.;
Clinics , 2012, DOI: 10.6061/clinics/2012(02)13
Abstract: objectives: the clinical significance of ischemia/reperfusion of the lower extremities demands further investigation to enable the development of more effective therapeutic alternatives. this study investigated the changes in the vascular reactivity of the rabbit femoral artery and nitric oxide metabolites under partial ischemia/ reperfusion conditions following cilostazol administration. methods: ischemia was induced using infrarenal aortic clamping. the animals were randomly divided into seven groups: control 90 minutes, ischemia/reperfusion 90/60 minutes, control 120 minutes, ischemia/reperfusion 120/90 minutes, cilostazol, cilostazol before ischemia/reperfusion 120/90 minutes, and ischemia 120 minutes/cilostazol/ reperfusion 90 minutes. dose-response curves for sodium nitroprusside, acetylcholine, and the calcium ionophore a23187 were obtained in isolated femoral arteries. the levels of nitrites and nitrates in the plasma and skeletal muscle were determined using chemiluminescence. results: acetylcholine-and a23187-induced relaxation was reduced in the ischemia/reperfusion 120/90 group, and treatment with cilostazol partially prevented this ischemia/reperfusion-induced endothelium impairment. only cilostazol treatment increased plasma levels of nitrites and nitrates. an elevation in the levels of nitrites and nitrates was observed in muscle tissues in the ischemia/reperfusion 120/90, cilostazol/ischemia/reperfusion, and ischemia/ cilostazol/reperfusion groups. conclusion: hind limb ischemia/reperfusion yielded an impaired endothelium-dependent relaxation of the femoral artery. furthermore, cilostazol administration prior to ischemia exerted a protective effect on endotheliumdependent vascular reactivity under ischemia/reperfusion conditions.
Ondas ultra-s?nicas de alta freqüência causam disfun??o endotelial em artérias coronárias caninas epicárdicas
Discigil, Berent;King, R. Michael;Pearson, Paul J.;Capellini, Verena K.;Rodrigues, Alfredo José;Schaff, Hartzell V.;Evora, Paulo Roberto Barbosa;
Revista Brasileira de Cirurgia Cardiovascular , 2008, DOI: 10.1590/S0102-76382008000200007
Abstract: objective: application of ultrasound energy by an endarterectomy probe can facilitate the removal of atheromatous plaque, but the effect of this procedure on surrounding vessel structure and function is still a matter of experimental investigations. methods: to determine whether ultrasound energy impairs the production of nitric oxide or damages vascular smooth muscle function, isolated canine epicardial coronary artery segments were exposed to either high (25 w) or low (0-10 w) ultrasonic energy outputs, for 15 seconds, using an endarterectomy device prototype. after exposure, segments of epicardial coronary artery were studied in organ chambers. the following drugs were used: adenosine diphosphate (adp), acetylcholine (ach) and sodium fluoride (naf) to study endothelium-dependent relaxation and sodium nitroprusside (snp) and isoproterenol to evaluate endothelium-independent relaxation. results: application of high ultrasonic energy power impaired endothelium-dependent relaxation to adp (10-9 - 10-4 m), ach (10-9 - 10-4 m) and naf (0.5 - 9.5 mm) in epicardial coronary arteries. however, low ultrasound energy output at the tip of the probe did not alter the endothelium-dependent relaxation (either maximal relaxation or ec50) to the same agonists. vascular smooth muscle relaxation to isoproterenol (10-9 - 10-5 m) or snp (10-9 - 10-6 m) was unaltered following exposure to either low or high ultrasonic energy outputs. conclusion: these experiments currently prove that ultrasonic energy changes endothelial function of epicardial coronary arteries at high power. however, ultrasound does not alter the ability of vascular smooth muscle of canine epicardial coronary arteries to relax.
Massage therapy in the management of myogenic TMD: a pilot study
Capellini, Verena Kise;Souza, Gisela Soares de;Faria, Cláudia Regina Sgobbi de;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000100005
Abstract: introduction: the temporomandibular disorder (tmd) is greatly prevalent in the population and can be associated with bruxism. this disorder produces several signs and symptoms. among them, pain is one of the most important because it reduces life quality and productivity of people who have such disorder. the aim of this research was to study if massage causes pain relief and/or electromyographic (emg) changes. materials and methods: the subjects were chosen by a questionnaire and divided into 2 different groups. their ages varied from 19 to 22 years. the experimental group consisted of 6 tmd patients, who were submitted to the massage treatment and 4 emg-sessions (the 1st emg-session occurred before the treatment and the others in the 1st, 15th and 30th days after the treatment). the control group consisted of 6 tmd patients, who were submitted to the same 4 emg-sessions. while emg activity was recorded, subjects were asked to keep mandibular rest position (mrp) and to perform maximal voluntary clenching (mvc). the treatment consisted of 15 massage-sessions on face and neck and in application of visual analogue scale (vas) for measuring pain level. the massage sessions had 30 minutes of duration and were performed daily. the emg data were processed to obtain the root mean square (rms), which were normalized by mvc. results: it was demonstrated that (1) rms-mrp of the right masseter in experimental group at the 1st emg-session was higher than at the 2nd emg-session and (2) statistically significant reduction was found for vas values after massage session. conclusion: unfortunately the sample is insufficient to draw any conclusions, therefore, more studies regarding the use of massage in the management of myogenic tmd are necessary.
Nitrite exhaled breath condensate study in patients undergoing cardiopulmonary bypass cardiac surgery
Augusto, Viviane dos Santos;Reis, Graziela Saraiva;Capellini, Verena Kise;Celotto, Andrea Carla;Rodrigues, Alfredo José;Evora, Paulo Roberto Barbosa;
Revista Brasileira de Cirurgia Cardiovascular , 2011, DOI: 10.1590/S0102-76382011000100006
Abstract: background: there is a relative lack of studies on postoperative changes in nitrite (no2 - ) concentrations, a marker of injury, following cardiac surgery. in this context, investigations on how exhaled no concentrations vary in the postoperative period of cardiac surgery will certainly contribute to new clinical findings. objective: the objective of this study was to compare the ebc no levels in both the pre and postoperative (24 hours) periods of cardiac surgery. methods: twenty - eight individuals were divided into three groups: 1) control, 2) coronary artery bypass grafting, and 3) valve surgery. the nitrite (no2 - ) levels were measured by chemiluminescence in blood samples and exhaled breath condensate (ebc). data were analyzed by the mann - whitney and wilcoxon tests. results: 1) preoperatively, the ebc no2 - levels from groups 2 and 3 patients were higher than control individuals; 2) the postoperative (24 hours) no2 - levels in the ebc from group 3 patients were lower compared with preoperative values; 3) the no2 - levels in the plasma from group 2 patients were lower in the preoperative compared with the postoperative (24h) values and; 4) preoperatively, there was no difference between groups 2 and 3 in terms of plasma no2 - concentrations. conclusion: these data suggest that no measurement in ebc is feasible in cardiac surgery patients.
Surgical Treatment of Endometrial Cancer  [PDF]
Lanowska Malgorzata, Brink-Spalink Verena, Hasenbein Kati, Schneider Achim, Marnitz Simone, K?hler Christhardt
Journal of Cancer Therapy (JCT) , 2010, DOI: 10.4236/jct.2010.14028
Abstract: Each year endometrial cancer is diagnosed in approximately 11.700 women in Germany. Operation is the therapy of choice in the primary treatment of patients with endometrial cancer. The traditional abdominal approach, vaginal, laparoscopic and robotic-assisted methods are available for the surgical treatment of EC today. This article compares and evaluates these different treatment options. With rising incidence of obesity, number of patients with endometrial cancer will also increase. However, operations in obese patients are more challenging. Laparotomy as standard therapy in endometrial cancer patients stage I and II should be replaced by laparoscopic approaches. Laparoscopy is oncologically adequate to open procedures and offers many advantages to patients. Robotic surgery in the treatment of endometrial cancer is still under evaluation. Most controversial points of treatment today are indication and extention of lymphadenectomy in different stages. In advanced tumor stages, optimal debulking should be performed in order to improve effectiveness of adjuvant chemotherapeutic and/or radiation therapy.
Methylene blue administration in the compound 48/80-induced anaphylactic shock: hemodynamic study in pigs
Menardi, Antonio Carlos;Capellini, Verena Kise;Celotto, Andrea Carla;Albuquerque, Agnes Afrodite Sumarelli;Viaro, Fernanda;Vicente, Walter Vilella A.;Rodrigues, Alfredo José;Evora, Paulo Roberto Barbosa;
Acta Cirurgica Brasileira , 2011, DOI: 10.1590/S0102-86502011000600013
Abstract: purpose: to verify if the methylene blue (mb) administration prevents and/or reverses the compound 48/80 (c48/80)-induced anaphylactic shock in pigs. methods: female dalland pigs were anesthetized and had the hemodynamic parameters recorded during the necessary time to administer some drugs and observe their effect. the animals were randomly assigned to one of the five groups: 1) control; 2) mb: the animals received a bolus injection of mb (2 mg/kg) followed by continuous infusion of mb (2.66 mg/kg/h delivered by syringe infusion pump); 3) c48/80: the animals received a bolus injection of c48/80 (4 mg/kg); 4) c48/80+mb: the animals received a bolus injection of c48/80 (4 mg/kg) and 10 minutes after the c48/80 administration the animals received a bolus injection of mb (2 mg/kg) followed by continuous infusion of mb (2.66 mg/kg/h delivered by syringe infusion pump); 5) mb+c48/80: the animals received a bolus injection of mb (2 mg/kg) and 3 minutes later they received a bolus injection of c48/80 (4 mg/kg). results: the intravenous infusion of mb alone caused no changes in the mean arterial pressure (map) showing that the administered mb dose was safe in this experimental model. the c48/80 was effective in producing experimental anaphylactic shock since it was observed a decrease in both map and cardiac output (co) after its administration. the mb did not prevent or reverse the c48/80-induced anaphylactic shock in this model. in fact, the map of the animals with anaphylactic shock treated with mb decreased even more than the map of the animals from the c48/80 group. on the other hand, the c48/80-induced epidermal alterations disappeared after the mb infusion. conclusion: despite our data, the clinical manifestations improvement brings some optimism and does not allow excluding the mb as a possible therapeutic option in the anaphylactic shock.
Oxidative stress is not associated with vascular dysfunction in a model of alloxan-induced diabetic rats
Capellini, Verena Kise;Baldo, Caroline Floreoto;Celotto, Andréa Carla;Batalh?o, Marcelo Eduardo;Cárnio, Evelin Capellari;Rodrigues, Alfredo José;Evora, Paulo Roberto Barbosa;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2010, DOI: 10.1590/S0004-27302010000600004
Abstract: objectives: to verify if an experimental model of alloxan-diabetic rats promotes oxidative stress, reduces nitric oxide bioavailability and causes vascular dysfunction, and to evaluate the effect of n-acetylcysteine (nac) on these parameters. methods: alloxan-diabetic rats were treated or not with nac for four weeks. plasmatic levels of malondialdehyde (mda) and nitrite/nitrate (nox), the endothelial and inducible nitric oxide synthase (enos and inos) immunostaining and the vascular reactivity of aorta were compared among diabetic (d), treated diabetic (td) and control (c) rats. results: mda levels increased in d and td. nox levels did not differ among groups. endothelial enos immunostaining reduced and adventitial inos increased in d and td. the responsiveness of rings to acetylcholine, sodium nitroprusside, and phenylephrine did not differ among groups. conclusions: nac had no effect on the evaluated parameters and this experimental model did not promote vascular dysfunction despite the development of oxidative stress.
Identification of Students at Risk for Attention Deficit Disorder with Hyperactivity Using the Response to Intervention Model (RTI)  [PDF]
Giseli Donadon Germano, Simone Aparecida Capellini
Psychology (PSYCH) , 2016, DOI: 10.4236/psych.2016.714169
Abstract: The use of Response to Intervention Model (RTI) has been described in the literature as an important tool for early identification of students at risk of learning problems. However, such studies are rare in the Brazilian population. This study aimed to use the response to intervention model (RTI) as an early identification procedure for Attention Deficit Disorder (ADD) students from 1st grade level of elementary school in Brazilian population. A total of 118 students participated of this study, aged between 6:0 and 6:11 (years:months), from 1st grade level, of both genders. The study was divided into three phases. In the first phase, all students were tested individually with Visual Attention Test software (TAVIS) composed of three tasks: selective attention, alternating attention and sustained attention. Because it was an initial study, all students were submitted to the Remediation Program with Attention and Working Memory (RAWM) in phase 2. The program consists of attention skills and phonological working memory skills (7 individual sessions, 40 minutes each). In phase 3, the students were submitted to the TAVIS. As results, from 118 students, 106 students concluded all 3 phases, which 34 students (32%) met the risk criteria for ADD after being submitted to the program. Of these, 34 students, 15 students (46.7%) responded to the intervention, while 19 students (59.3%) not responded. These students who not responded to the intervention program were sent to multidisciplinary care for monitoring and subsequent confirmation of the diagnosis of ADD. The findings of this study able us to conclude that RTI model can be used as an early identification tool for student at risk of attention in the early years literacy deficit.
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