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Search Results: 1 - 10 of 590 matches for " Veena Pande "
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Production, Purification, and Characterization of Polygalacturonase from Rhizomucor pusillus Isolated from Decomposting Orange Peels
Mohd. Asif Siddiqui,Veena Pande,Mohammad Arif
Enzyme Research , 2012, DOI: 10.1155/2012/138634
Abstract: A thermophilic fungal strain producing polygalacturonase was isolated after primary screening of 40 different isolates. The fungus was identified as Rhizomucor pusilis by Microbial Type Culture Collection (MTCC), Chandigarh, India. An extracellular polygalacturonase (PGase) from R. pusilis was purified to homogeneity by two chromatographic steps using Sephadex G-200 and Sephacryl S-100. The purified enzyme was a monomer with a molecular weight of 32?kDa. The PGase was optimally active at 55°C and at pH 5.0. It was stable up to 50°C for 120?min of incubation and pH condition between 4.0 and 5.0. The stability of PGase decreases rapidly above 60°C and above pH 5.0. The apparent and values were 0.22?mg/mL and 4.34?U/mL, respectively. It was the first time that a polygalacturonase enzyme was purified in this species. It would be worthwhile to exploit this strain for polygalacturonase production. Polygalacturonase from this strain can be recommended for the commercial production because of its constitutive and less catabolically repressive nature, thermostability, wide range of pH, and lower properties. However, scale-up studies are needed for the better output for commercial production. 1. Introduction Pectin substances constitute a complex linear backbone comprised of α-1, 4-linked d-galacturonic acid residues which may be methylated and substituted with l-rhamnose, arabinose, galactose, and xylose [1–3]. Because of the large variety of pectins in plant material, they endowed with many pectinolytic enzyme systems which can degrade them [4]. The hydrolysis of pectin backbone is obtained by the synergistic action of several enzymes, including pectin methylesterase (EC. 3.1.11.1), endopolygalacturonase (EC. 3.2.1.15), exopolygalacturonase (EC. 3.2.1.67), pectate lyase (EC. 4.2.2.2), exo-pectate lyase (EC. 4.2.2.9), and endopectin lyase (4.2.2.10) [5, 6]. Pectinolytic enzymes are of prime importance for plants as they help in cell-wall extension and softening of some plant tissues during maturation and storage [7, 8]. They also aid in maintaining ecological balance by causing decomposition and recycling of waste plant materials. Plant pathogenicity and spoilage of fruits and vegetables by rotting are some other major manifestations of pectinolytic enzymes [9, 10]. They have been used in many industrial and biotechnological processes, such as textile and plant fiber processing, coffee and tea fermentation, oil extraction, treatment of industrial wastewater containing pertinacious material, purification of plant viruses, and paper making [11]. Commercial enzyme
HPLC Evaluation of Phenolic Profile, Nutritive Content, and Antioxidant Capacity of Extracts Obtained from Punica granatum Fruit Peel
Sushil Kumar Middha,Talambedu Usha,Veena Pande
Advances in Pharmacological Sciences , 2013, DOI: 10.1155/2013/296236
Abstract: This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts of Punica granatum peel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185?±?12.45 to 298.00?±?24.86?mg GAE (gallic acid equivalents)/gm and 23.05?±?1.54 to 49.8?±?2.14 quercetin (QE) mg/g, respectively. The EC50 of herbal extracts ranged from 100 μg/ml (0.38 quercetin equivalents), for AQPE, 168 μg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry. 1. Introduction Nature still obliges as the man’s primary source for the cure of his ailments. Research in preventive medicine showed the importance of functional nutrition in reducing the risk factor of certain chronic diseases. Innate defense system of the human body may be insufficient for the damage caused by continued oxidative stress [1]. Reactive oxygen species (ROS) are, in general, considered to be cytotoxic and are implicated in the progression of cancer, inflammation, radiation injury, and aging [2]. Growing scientific evidence has shown adverse side effects, like liver damage and mutagenesis, of synthetic antioxidants [3]. Therefore, recently there has been an upsurge of interest in natural products as antioxidants, as they inhibit the free radical reactions and protect human body from various diseases, such as cancer and diabetes [4]. Recent studies showed that a number of plant products including polyphenolic substances (e.g., gallocatechins, delphinidin, cyanidin, gallic acid, ellagic acid, pelargonidin and sitosterol) and various plants or herbal extracts exert potent antioxidant actions, which are very well known for their healing powers [5]. The
Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests
Kumar Navin,Singh Jai PN,Pande Veena,Mishra Neelima
Malaria Journal , 2012, DOI: 10.1186/1475-2875-11-298
Abstract: Background Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates. Methods One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined. Results Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs. Conclusion The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.
Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species
DINESH S. BISHT,RAJENDRA C. PADALIA,LALIT SINGH,VEENA PANDE
Journal of the Serbian Chemical Society , 2010,
Abstract: The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook. f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial β-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans,trans nepetalactone (83.4 %) and isoiri-domyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and β-caryophyllene (18.6 %), while N. clarkei was dominated by β-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.
Molecular Docking and Quantum Mechanical Studies on Pelargonidin-3-Glucoside as Renoprotective ACE Inhibitor
Talambedu Usha,Pranav Tripathi,Veena Pande,Sushil Kumar Middha
ISRN Computational Biology , 2013, DOI: 10.1155/2013/428378
Abstract: Background and Aim. Despite tangible progress in recent years, substantial therapeutic challenges remain unexplored in nephropathy, particularly in diabetic patient. Addressing these challenges requires identification of novel drugs and development of noninvasive and cost-effective methods to select the most appropriate therapeutic option for the disease. Angiopathic nephropathy is one of the complications of diabetes mellitus and is becoming the single most important reason for end-stage renal disease in the western world. This study has investigated the inhibitory effect of a library naturally occurring nonprotein compounds that inhibit angiotensin converting enzyme (ACE). Materials and Methods. Docking studies of ACE protein with natural compounds and synthetic commercial drug perindopril were done using AutoDock, FlexX, and Hex. Toxicity predictions were carried out using OpenTox. Quantum mechanical properties were studied using GAMESS. Results. Pelargonidin-3-glucoside could be used as a potent renoprotective drug candidate, which inhibits ACEII. It has low toxicity and its quantum mechanical properties are comparable to those of commercial drugs. 1. Introduction Angiotensin converting enzyme (ACE) is an active participant in the body’s renin-angiotensin-aldosterone system for mediation of extracellular volume and arterial vasoconstriction. Secretion of this enzyme is carried by pulmonary and renal endothelial cells to catalyze the conversion of decapeptide angiotensin I to octapeptide angiotensin [1]. Apart from the normal catalysis activity, ACE also degrades bradykinin thus acting as a vasoconstrictor [2]. These two actions make ACE inhibition a potent target in the treatment of conditions such as hypertension, heart failure, and diabetic nephropathy. Inhibition of ACE results in the dwindled formation of angiotensin II and reduced catabolism of bradykinin, leading to systematic dilation of the arteries and veins and a decrease in arterial blood pressure. Inhibition of this enzyme also decreases the risk of end-stage renal disease (ESRD) in diabetics [3]. perindopril is a synthetic chemical drug which inhibits ACE; however, it causes dysfunctioning of the left ventricle of in Duchenne muscular dystrophy [4]. Considering the side effects of perindopril, the present study investigates the inhibitory effect of natural compounds in comparison with perindopril by insilico approach. 2. Materials and Methods To investigate the protein ligand interaction, the natural compounds and perindopril were docked into the active site of ACE predicted by pocket
Variation in Wood Properties and Growth in Some Clones of Populus deltoides Bartr. ex Marsh  [PDF]
P. K. Pande
American Journal of Plant Sciences (AJPS) , 2011, DOI: 10.4236/ajps.2011.25076
Abstract: The present paper deals with within ramet radial, intra- and inter-clonal variations in the wood element’s dimensions and specific gravity of 10 clones of Populus deltoides Bartr. ex Marsh. The growth parameters namely ramet height and DBH were also considered for the study. Study material was collected from the 10 clones of Populus deltoids raised by WIMCO Plantations Ltd. at Rudrapur (Udhamsingh Nagar), India. Three clones were parent viz. G48, S7C8 (female) and G3 (male). Other clones represent hybrids of F1 generation. Inter- and intra-clonal variations were significant for all the wood traits except vessel element length for intra-clonal variations. Within ramet variations due to radial location were significant for fiber length and specific gravity with increasing trend from pith to periphery. Interaction of clone*replication was also significant for all the wood traits. Variations were significant for the DBH for the clones. Fiber length and specific gravity was significantly higher in female while wall thickness and vessel element length was in male clones (P < 0.01). Female parents (G48 and S7C8) showed higher fiber length and specific gravity than of the male parent (G3) while vessel element diameter and wall thickness was higher in male parent (G3). Fiber length was higher in offspring than the parent clones which may be the reflection of hybrid vigor for the trait. The clones of F1 offspring followed the similar patterns for the other wood traits as in the parent clones. Hierarchical cluster analysis showed that W/A 39 (male) and W 39 (female) clones of F1 generation were highly divergent than of the other clones.
Unravelling Effects of Temperature and Soil Moisture Stress Response on Development of Dry Root Rot [Rhizoctonia bataticola (Taub.)] Butler in Chickpea  [PDF]
Mamta Sharma, Suresh Pande
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43076
Abstract: Erratic rainfalls and rise in temperature have become more frequent under the changing scenario of climate particularly in semiarid tropics. As a consequence of it, a drastic shift of chickpea diseases have been recorded throughout the major chickpea growing regions in India and elsewhere. Dry root rot (DRR) caused by Rhizoctonia bataticola (Taub.) Butler [Pycnidial stage: Macrophomina phaseolina (Tassi) Goid] was found as a potentially emerging constraint to chickpea production than wilt (Fusarium oxysporum f. sp. ciceris). Increasing incidence of DRR indicate strong influence of climate change variables such as temperature and moisture on the development of disease. The present study therefore was conducted to quantify the role of temperature and soil moisture associated with infection, colonization and development of DRR under controlled environment. The DRR incidence was significantly affected by high temperature and soil moisture deficit. Out of five temperature regimes (15?C, 20?C, 25?C, 30?C and 35?C) and four moisture
Electron-Proton Pairing at High Temperatures, Solar Flares, and the FIP Effect  [PDF]
L. K. Pande
Journal of Modern Physics (JMP) , 2016, DOI: 10.4236/jmp.2016.71003
Abstract: We analyze the line data from solar flares to present evidence for the emission spectrum of the recently discussed electron-proton pairs at high temperatures. We also point out that since the pairing phenomenon provides an additional source for these lines—the conventional source being the highly ionized high-Z atoms already existing in the solar atmosphere, we have a plausible explanation of the FIP effect.
Cultural Identity and Human Rights: Minority Claims, Ethnic Identity and Group Rights  [PDF]
Dhruv Pande, Munmun Jha
Open Journal of Political Science (OJPS) , 2016, DOI: 10.4236/ojps.2016.64032
Abstract: This paper explores the relationship between cultural identity and human rights in the light of the dynamics of identity formation, based on the immediate external culture of any multicultural society. The objective of this study is to analyze the relationship between culture, identity and rights, which broadens the parameter of the existing dimension of human rights, using secondary sources to review the literature. It argues that these dynamics are marked by a number of factors and components featuring the group, community and individual rights. This prepares grounds for a wider, inclusionary and horizontal understanding of the Human Rights dimension and paradigms, not only in a multicultural society but also in a democratic nation- state which is significantly marked by minority rights and ethnic identity claims. Thus through a critical approach and a post-colonial perspective, this paper shows how this specific and particular dynamics of cultural identity casts an effect on the theory and practice of normative political theory and trajectories. The paper concludes that the Human Rights inherently invoke a challenge in the analysis of identity-formation and cultural heterogeneity dynamics, which are significant in the contemporary global democratic politics.
On the Quadratic Transportation Problem  [PDF]
Veena Adlakha, Krzysztof Kowalski
Open Journal of Optimization (OJOp) , 2013, DOI: 10.4236/ojop.2013.23012
Abstract:

We present a direct analytical algorithm for solving transportation problems with quadratic function cost coefficients. The algorithm uses the concept of absolute points developed by the authors in earlier works. The versatility of the proposed algorithm is evidenced by the fact that quadratic functions are often used as approximations for other functions, as in, for example, regression analysis. As compared with the earlier international methods for quadratic transportation problem (QTP) which are based on the Lagrangian relaxation approach, the proposed algorithm helps to understand the structure of the QTP better and can guide in managerial decisions. We present a numerical example to illustrate the application of the proposed method.

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