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Search Results: 1 - 10 of 4617 matches for " Vanessa FZ Marinho "
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Lymph vascular invasion in invasive mammary carcinomas identified by the endothelial lymphatic marker D2-40 is associated with other indicators of poor prognosis
Vanessa FZ Marinho, Konradin Metze, Fernanda SF Sanches, Gislene FS Rocha, Helenice Gobbi
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-64
Abstract: We selected 123 cases of invasive mammary carcinomas stratified into three subgroups according to axillary lymph node status: macrometastases, micrometastases, and lymph node negative. Lymphatic (LVI) and blood (BVI) vessel invasion were evaluated by H&E and immunohistochemistry using the D2-40 and CD31 antibodies, and related to histologic tumor type and grade, estrogen and progesterone receptors, E-cadherin, Ki67, p53, and Her2/neu expression.LVI was detected in H&E-stained sections in 17/123 cases (13.8%), and in D2-40 sections in 35/123 cases (28.5%) (Kappa = 0.433). BVI was detected in H&E-stained sections in 5/123 cases (4.1%), and in CD31 stained sections in 19/123 cases (15.4%) (Kappa = 0.198). LVI is positively related to higher histologic grade (p = 0.013), higher Ki67 expression (p = 0.00013), and to the presence of macrometastases (p = 0.002), and inversely related to estrogen (p = 0.0016) and progesterone (p = 0.00017) receptors expression.D2-40 is a reliable marker of lymphatic vessels and is a useful tool for lymphatic emboli identification in immunostained sections of breast carcinomas with higher identification rates than H&E. Lymphatic vessel invasion was related to other features (high combined histologic grade, high Ki67 score, negative hormone receptors expression) associated with worse prognosis, probable reflecting a potential for lymphatic metastatic spread and aggressive behavior.Lymphatic vessels are considered the main route by which tumor cells reach axillary lymph nodes [1-3]. Lymphatic vessel invasion (LVI) is known as an independent predictor of lymph node metastases in breast cancer. The diagnosis of LVI is made based on the presence of tumor emboli within vascular channels lined by a single layer of endothelial cells without red blood cells. Lymphatic vessels are flattened channels or open spaces lined by a single layer of endothelial cells whose lumen are sometimes filled with lymphocytes. However, the identification of LVI is diffi
Attempt at the determination of aluminum nitrate LD50 and the study of its neurotoxicological effect in Wistar rat
FZ Azzaoui
Biology and Medicine , 2012,
Abstract: Values of oral lethal dose of aluminum (Al) nitrate are extremely different and rare in the literature. The study is an attempt to determine the oral LD50 of aluminum nitrate in the Wistar rats and to measure the effect of the high doses of this aluminum compound on rats’ different organs, on acetylcholinesterase (AChE) activity, and on acetylcholine (ACh) levels in the hippocampus. Four groups of male Wistar rats are used (n = 28). The treated groups receive three doses of aluminum nitrate (Al1 = 2,500 mg/kg, Al2 = 3,500 mg/kg, and Al3 = 4,500 mg/kg) once by gavage, while control rats receive tap water. All rats are examined twice daily for mortality and impairment during the 2-week experiment. The bodyweight (BW) is measured at the beginning and at the end of the experiment. Dissection is realized for each dyed rat and the dosage of AChE activity and ACh levels isrealized at the end of the experiment by colorimetric method. The obtained results show that the higher dose (Al3) kills 30% of the rats under study and causes spleens’ dark discoloration in the dyed rats. Both Al2 and Al3decrease significantly the spleen weight (p < 0.01) and AChE activity (p < 0.01), but increase significantly the ACh levels (p < 0.01 and p < 0.001, respectively), in hippocampus of rats. Even if the lethal dose of aluminum nitrate is not reached, the effect of the high acute doses on viscera and cholinergic system is demonstrated.
FZ Zainal
Malaysian Family Physician , 2007,
Malaysian Family Physician , 2008,
Schistosomal myeloradiculopathy due to Schistosoma mansoni: report on 23 cases
Nobre, Vandack;Silva, Luciana CS;Ribas, Jo?o G;Rayes, Abdunnabi;Serufo, JC;Lana-Peixoto, MA;Marinho, Rosana FZ;Lambertucci, JR;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000900020
Abstract: schistosoma mansoni infection is likely to be responsible for a significant proportion of cases of myelopathy occurring in areas where schistosomiasis is endemic. the aim of this study is to describe the clinical, laboratory and therapeutic data of 23 patients with schistosomal myeloradiculopathy. the medical records of 23 patients with schistosomal myelopathy admitted to two general hospitals of belo horizonte (mg), in brazil, from 1995 to 1999, were reviewed retrospectively. seventeen patients were male (74%). the mean age for the whole group was 27 years. lower limb weakness and associated lumbar and/or lower limb pain were reported by 20 patients (87%), and 16 (70%) were unable to walk. all individuals presented urinary retention and 19 (83%) complained of intestinal dysfunction. the treatment was based on the association of antischistosomal drugs and corticosteroids. five patients (22%) presented a full response to treatment, 13 (57%) partial response without functional limitations and 4 (17%) partial improvement with limitations or no response. three out of the 4 patients who stopped steroids before 45 days of treatment developed recurrence of the symptoms and signs of myelopathy. our cases demonstrate the severe presentation of the disease and the data disclosed here suggest that treatment with steroids should be kept for months after clinical improvement.
Micrometástases de carcinoma da mama em linfonodos axilares: detec??o por imunoistoquímica versus hematoxilina e eosina
Marinho, Vanessa Fortes Zschaber;Zagury, Marcos Salom?o;Caldeira, Lidiane Gomes;Gobbi, Helenice;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2004, DOI: 10.1590/S1676-24442004000200012
Abstract: introduction: the methods of detection and prognostic significance of micrometastasis (mic-met) are still controversial in the literature. aims: the aim of our study was to compare micrometastasis detection of invasive mammary carcinomas (imc) in axillary lymph nodes using a second review (double review) of hematoxylin and eosin (he) stained slides using immunohistochemistry (ihc) and the impact of micrometastasis detection in re-staging patients. material and methods: we studied 190 cases of imc with no axillary metastasis described in the original reports. we reviewed the available he stained slides of lymph nodes. new sections were obtained from archived paraffin blocks and were submitted for ihc using the pancytokeratin antibody ae1/ae3 and the streptavidin biotin peroxidase method. results: we reviewed 2868 lymph nodes stained by he (mean = 15.1 lymph nodes/patient) and 2.444 lymph nodes stained by ihc (mean = 12.9 lymph nodes/patient). micrometastasis were detected in 28/190 cases, (by he in 14/190 cases, 7.4%, and by ihc in 25/190 cases, 13.2%). the second review of the he stained slides showed good specificity (98.2%), but low sensitivity (44%), when compared to ihc (considered the gold standard). conclusions: the detection of micrometastasis was better using immunohistochemistry than second review, and changed the stage of 28 patients (14.7%).
Micrometástases de carcinoma da mama em linfonodos axilares: detec o por imunoistoquímica versus hematoxilina e eosina
Marinho Vanessa Fortes Zschaber,Zagury Marcos Salom?o,Caldeira Lidiane Gomes,Gobbi Helenice
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2004,
Abstract: INTRODU O: Os métodos de detec o e o significado prognóstico das micrometástases (Mic-Met) em linfonodos axilares (LA) de pacientes com carcinoma mamário invasor s o controversos na literatura. OBJETIVOS: Comparar a detec o de micrometástases de carcinoma mamário em LA através de segunda revis o de laminas coradas por hematoxilina e eosina (HE) e comparar com a detec o imunoistoquímica (IHQ) e seu impacto no restadiamento das pacientes. MATERIAIS E MéTODOS: Foram estudados 190 casos de carcinoma mamário inicialmente diagnosticados como linfonodo-negativos, com reavalia o dos linfonodos em laminas coradas por HE e IHQ para pancitoqueratina (clone AE1/AE3) e método streptavidina-biotina-peroxidase (LSAB+). RESULTADOS: Foram revistos 2.868 linfonodos corados por HE (média = 15,1 linfonodos/paciente) e 2.444 linfonodos corados por IHQ (média = 12,9 linfonodos/paciente). Micrometástases foram detectadas em 28/190 casos, sendo que a detec o por IHQ (25/190 casos; 13,2%) foi superior à detec o por HE (14/190 casos; 7,4%). A revis o de laminas coradas por HE apresentou boa especificidade (98,2%), mas baixa sensibilidade (44%) em rela o à IHQ (considerada padr o-ouro). Conclus o: A detec o de Mic-Met foi maior por imunoistoquímica do que por segunda leitura de laminas, e gerou mudan a no estadiamento de 28 pacientes (14,7%).
Filtration and ingestion rates of Thalassiosira Weissflogii (Bacillariophyta) by Euterpina acutifrons Dana (Copepoda)
Vanessa Oliveira Lemos,Rauquírio Marinho da Costa,Luci Cajueiro Carneiro Pereira
Boletim do Museu Paraense Emílio Goeldi : Ciências Naturais , 2006,
Abstract: Short-term experiments were carried out to study the feeding performances of the copepod Euterpina acutifrons fed on Thalassiosira weissiogii. Four adult females of this species were sorted and transferred to 15 ml glass bottles filled with different dilutions of the diatom cultures to obtain cell concentrations of 7.5, 10.0, 12.5, 15.0, 17.5, 20.0, 22.5 and 25.0 x 103 cell.ml-1 (8 treatments, 3 replicates, 4 copepods/replicate). Initial and final cell concentrations of control and experimental bottles were determined using a Neubauer Chamber. Filtration and ingestion rates were measured thought 1 hour grazing trials performed under controlled laboratory conditions. Filtration rates of Euterpina acutifrons oscillated from 0.81 to 2.92 ml. copepod-1 .h-1 and they significantly higher (p<0.05) on 7.5 x 103 cell.ml-1 concentration. Ingestion rates varied between 1.8 and 8.13 x 103 cell.copepod-1.h-1, showing significative differences (p<0.05) between treatments with low (7.5 x 103 cell.ml-1) and high (22.0 x 103 cell.ml-1) number of cells. The feeding rates of Euterpina acutifrons fed on Thalassiosira weissüogii revealed the important hole of this species of copepod on the aquatic food web of coastal ecosystems of the Amazonian region, especially on the northeast of Pará where it is commonly found.
The Effects of Isosorbide Dinitrate on in Vitro Proliferation of WEHI-164 Cells and Peripheral Blood Mononuclear Cells
Hajighasemi F,Resvan Madani FZ
Tehran University Medical Journal , 2012,
Abstract: Background: Isosorbide dinitrate has been broadly used in the treatment of various ischemic heart diseases. Isosorbide is a nitric oxide donor which increases blood flow to tumors through vasodilatation and consequently accelerates the access of chemo-drugs to them. Furthermore, this drug has inhibitory effects on angiogenesis, tumor growth and metastasis in vivo. Moreover, its ant-inflammatory effects have also been reported. In the present study we evaluated the effects of isosorbide on the proliferative activity of fibrosarcoma WEHI-164 cell line and peripheral blood mononuclear cells (PBMCs). Methods: WEHI-164 fibrosarcoma cells and human PBMCs were cultured in complete Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal bovine serum and 2×104 cells/mL for WEHI-164 and 2×105 cells/mL for PBMCs. The cells were then incubated at the exponential growth phase with different concentrations of isosorbide (4×10-6-1.6×10-3 M) for 24, 48 and 72 hours. Subsequently, isosorbide effects on proliferation of the cells were evaluated by trypan blue dye exclusion (TB) test and MTT assay. Statistical comparisons between groups were made by analysis of variance. Results: The proliferative activity of WEHI-164 fibrosarcoma cells and human PBMCs treated with different concentrations of isosorbide, did not show any significant difference with untreated control cells. Conclusion: The results of this study showed that isosorbide neither had any significant effects on the proliferative activity of fibrosarcoma WEHI-164 cells nor on human PBMCs. Our findings suggest that anti-tumoral effects of isosorbide reported by other investigators may be mediated through non-cytotoxic mechanisms.
Varia??o temporal do fitoplancton e dos parametros hidrológicos da zona de arrebenta??o da Ilha Canela (Bragan?a, Pará, Brasil)
Sousa, Eliane Brabo de;Costa, Vanessa Bandeira da;Pereira, Luci Carneiro Cajueiro;Costa, Rauquírio Marinho da;
Acta Botanica Brasilica , 2009, DOI: 10.1590/S0102-33062009000400018
Abstract: seasonal and nyctemeral variations of the phytoplankton and hydrological parameters were studied at a fixed station (00o46'37.2"s-046o43'24.5"w) situated in canela island (bragan?a-pará) in september and december, 2004 and in march and june, 2005. subsurface water samples were taken for qualitative and quantitative phytoplankton studies and to determine chlorophyll a concentrations. simultaneously physical and chemical parameters were measured: salinity, temperature, ph, dissolved oxygen and percent of oxygen saturation. a total of 64 taxa belonging to cyanophyta (one taxon), bacillariophyta (54 taxa) and dinophyta (nine taxa) were identified. chlorophyll a concentrations ranged from 4.67 mg m-3 (dry season), to 5.44 mg m-3 (rainy season) and follow phytoplankton densities, which were higher during the rainy period (mean values of 1,870 x 103 cell l-1). phytoflagellates quantitatively dominated the local phytoplanktonic community followed by diatoms. dimeregramma minor and skeletonema sp. were abundant and very frequent species over the studied period. resuspension processes originated by the strong winds and waves in the surf zone favored the dominance of d. minor during the dry period. in the rainy period, high rainfall, moderate winds as well as the influence of estuarine waters from tapera?u and caeté propitiated the decrease of salinity and the development of other phytoplankton species.
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