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Search Results: 1 - 10 of 47 matches for " Vana Kolovou "
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Association of gender, ABCA1 gene polymorphisms and lipid profile in Greek young nurses
Vana Kolovou, Apostolia Marvaki, Agathi Karakosta, Georgios Vasilopoulos, Antonia Kalogiani, Sophie Mavrogeni, Dimitrios Degiannis, Christina Marvaki, Genovefa Kolovou
Lipids in Health and Disease , 2012, DOI: 10.1186/1476-511x-11-62
Abstract: The study population consisted of 447 (87 men) unrelated nurses who were genotyped for ABCA1 gene polymorphisms. Additionally, lipid profile [total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol (LDL-C) and apolipoprotein A1] was evaluated.The distribution of all three studied ABCA1 gene polymorphisms did not differ according to gender. However, only R219K genotype distribution bared borderline statistical significance (p?=?0.08) between the two studied groups. Moreover, allele frequencies of R219K, R1587K and I88M polymorphisms did not differ according to gender. In general, blood lipid levels did not seem to vary according to ABCA1 gene polymorphisms, when testing all subjects or when testing only men or only women. However, a significant difference of LDL-C distribution was detected in all subjects according to R1587K genotype, indicating lower LDL-C levels with KK polymorphism (p?=?0.0025). The above difference was solely detected on female population (p?=?0.0053).The ABCA1 gene polymorphisms frequency, distribution and lipid profile did not differ according to gender. However, in the female population the KK genotype of R1587K gene indicated lower LDL-C levels. Further studies, involving a higher number of individuals, are required to clarify genes and gender contribution.ATP-binding cassette transporter A1 (ABCA1) mediates the transport of cholesterol and phospholipids from cells to lipid-poor apolipoproteins. Animals and human studies documented that defects in the ABCA1 pathway are significant determinants of coronary artery disease (CAD) [1]. Inactivation of ABCA1 gene in macrophages increases atherosclerotic lesions in hyperlipidemic mice [2,3], and overexpressing human ABCA1 in transgenic mice retards atherogenesis [4,5]. The ABCA1 gene is located on the chromosome 9 in the area 9q31.1 and encodes ABCA1 protein. ABCA1 protein is expressed in liver, macrophages, intestines, lungs etc. Several ABCA1 gen
Platelet activating factor levels and metabolism in tangier disease: a case study
Vana Kolovou, Vasiliki D Papakonstantinou, George Stamatakis, Sophia N Verouti, Marianna N Xanthopoulou, Genovefa Kolovou, Constantinos A Demopoulos
Lipids in Health and Disease , 2012, DOI: 10.1186/1476-511x-11-89
Abstract: The EC50 value of PRP was measured by an aggregometer. The determination of the specific activity of PAF-CPT and Lyso-PAF-AT was made after in vitro enzymatic assay, chromatographic separation and measurement of the produced PAF in a biological assay with washed rabbit platelets. The determination of PAF-AH and Lp-PLA2 was made after an in vitro enzymatic assay from the decay of radioactive PAF.The TD patient had lower bound-PAF values in blood, decreased specific activity of PAF-CPT and Lyso-PAF-AT, increased specific activity of PAF-AH in platelets and leukocytes and Lp-PLA2 activity in plasma compared to healthy women. The EC50 of PAF and Thrombin were higher compared to healthy women.The increased Lp-PLA2 activity, as well as, the decreased activities of PAF-CPT and Lyso-PAF-AT, explain the decreased bound-PAF level in TD patient and the EC50 of PAF. However, total PAF is in a normal range and this probably can explain one of the reasons this TD patient has no CAD.
ATP-binding cassette transporter A1 gene polymorphisms and serum lipid levels in young Greek nurses
Vana Kolovou, Genovefa Kolovou, Apostolia Marvaki, Agathi Karakosta, Georgios Vasilopoulos, Antonia Kalogiani, Dimitrios Degiannis, Christina Marvaki, Constantinos A Demopoulos
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-56
Abstract: The study population consisted of 308 unrelated nurses who were genotyped and the ABCA1 polymorphisms were detected. Additionally, lipid profile [total cholesterol (TC), triglycerides (TGs), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and apolipoprotein (apo) A] was evaluated.There was no difference in the genotypic and allelic frequencies of the R219K polymorphism according to lipid profile. The R1587K genotypes differed significantly according to TC, LDL-C and TGs concentration (p = 0.023, p = 0.014 and p = 0.047, respectively). Particularly, significant difference in TC, LDL-C and TGs concentration was detected between RK and RR genotypes (p = 0.006, p = 0.004, p = 0.014, respectively). Women with RK genotype compared to RR genotype had higher concentration of TGs (134.25 mg/dl vs 108.89 mg/dl, p = 0.014, respectively), total cholesterol (207.41 mg/dl vs 187.69 mg/dl, p = 0.006, respectively), and LDL-C (110.6 mg/dl vs 96.9 mg/dl, p = 0.004, respectively).These findings suggest that the R1587K polymorphism of ABCA1 gene was associated with lipid profile of Greek nurses. Women with RK genotype had higher TGs, total and LDL-C concentration compared to RR genotype. These observations may be significant in assessing the risk of CAD since a 1% change in LDL-C is associated with a 1% change of cardiovascular events. Also, TGs concentration were documented to play a significant role in women. However, this needs to be confirmed by larger studies.The ATP-binding cassette transporter A1 (ABCA1) acts as a vehicle for cellular cholesterol which after crossing cell membrane bounds to acceptor molecule such as apolipoprotein (apo) A [1-3]. Thus, ABCA1 influences the initial steps in high density lipoprotein (HDL) formation and in reverse cholesterol transport. The ABCA1 protein belongs to ABC proteins family, which are ingredients of biological membranes and use ATP to transfer various particles such as lipids [1]. The ABCA1 prote
Severe/Extreme Hypertriglyceridemia and LDL Apheretic Treatment: Review of the Literature, Original Findings
Olga Diakoumakou,Georgios Hatzigeorgiou,Nikos Gontoras,Maria Boutsikou,Vana Kolovou,Sophie Mavrogeni,Vassiliki Giannakopoulou,Genovefa D. Kolovou
Cholesterol , 2014, DOI: 10.1155/2014/109263
Abstract: Hypertriglyceridemia (HTG) is a feature of numerous metabolic disorders including dyslipidemias, metabolic syndrome, and diabetes mellitus type 2 and can increase the risk of premature coronary artery disease. HTG may also be due to genetic factors (called primary HTG) and particularly the severe/extreme HTG (SEHTG), which is a usually rare genetic disorder. Even rarer are secondary cases of SEHTG caused by autoimmune disease. This review considers the causes of SEHTG, and their management including treatment with low density lipoprotein apheresis and analyzes the original findings. 1. Introduction A positive correlation between high triglycerides (TGs) concentration and coronary heart disease (CHD) has been established in numerous studies [1–11]. Hypertriglyceridemia (HTG) is prevalent in 18.6% of men and 4.2% of women between 16 and 65 years of age. The Adult Treatment Panel (ATP) III guidelines, published 13 years ago [12], described normal TGs concentration <150?mg/dL (<1.6?mmol/L), borderline-high TGs as 150 to 199?mg/dL (1.6–2.2?mmol/L), high TGs as 200 to 499?mg/dL (2.2–5.6?mmol/L), and very high TGs as >500?mg/dL (>5.6?mmol/L). However, severe/extreme hypertriglyceridemia (SEHTG) should be considered when values are greater than 1,000?mg/dL (11.2?mmol/L) because this places individuals at significant increased risk of pancreatitis. With TG values less than 1,000?mg/dL (5.6?mmol/L) one should be focused on the risk of premature CHD [13]. HTG is a feature of numerous metabolic disorders including dyslipidemias, metabolic syndrome, and diabetes mellitus type 2 (DMT2) and can increase the risk of premature CHD [14, 15]. These metabolic disorders may be caused by interactions between genetic and nongenetic factors since those subjects present usually similar clinical features (android type of obesity, ectopic fat deposition, thin arms and legs, increased waist circumference, upper body obesity, and in case of SEHTG eruptive xanthomas) [16, 17]. Visceral fat is considered to behave as ectopic fat deposition. It accumulates TGs in cases when body fat storage exceeds the capacity of fat stores. Furthermore, subjects with HTG usually present insulin resistance, hepatic steatosis, and DMT2. Thus, all the above can be called “hypertriglyceridemic phenotype.” Additionally, several studies (including ours) showed that postprandial HTG is manifested in subjects with hypertriglyceridemic phenotype [18]. HTG may also be due to genetic factors (called primary HTG) and particularly the SEHTG, which is a usually rare genetic disorder. Even rarer are secondary
The role of common variants of the cholesteryl ester transfer protein gene in left main coronary artery disease
Genovefa Kolovou, Ioannis Vasiliadis, Vana Kolovou, Agathi Karakosta, Sophie Mavrogeni, Evaggelia Papadopoulou, Spiridon Papamentzelopoulos, Vasiliki Giannakopoulou, Apostolia Marvaki, Dimitrios Degiannis, Helen Bilianou
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-156
Abstract: The DNA of 471 subjects [133 subjects with angiographically documented left main coronary artery disease (LMCAD), 241 subjects with more peripheral coronary artery disease (MPCAD) and 97 subjects self reported healthy (Controls)] was analyzed for the frequency of TaqIB and I405V polymorphisms in the gene coding CETP.There is no significant difference in CETP allele frequency or genotype distribution among LMCAD and MPCAD patients although there is statistical difference between LMCAD and Controls (p = 0.001). Specifically, patients with LMCAD and B1B1 genotype of TaqIB polymorphism were more frequent present compared to Controls (33.8% vs 22.9%, respectively). The frequency of B2B2 genotype was 3 times lower in the LMCAD group compared to Controls (10.5% vs 30.2%, respectively). In the LMCAD group the frequency of B1 allele compared to Controls was higher (62% vs 46%, respectively, p = 0.001). The relationship between TaqIB gene polymorphism and the LMCAD was independent of lipid profile, with the exception of apolipoprotein A.These findings indicate that the TaqIB polymorphism may have potential importance in screening individuals at high risk for developing CAD. However, this polymorphism cannot distinguish between LMCAD and MPCAD. Further prospective investigations in larger populations are required to confirm these findings.The evolution of coronary artery disease (CAD) is influenced by various genetic and environmental factors. The genetic contribution is documented by a positive family history for myocardial infarction and is considered to be a strong cardiovascular risk factor [1,2]. This has been supported even after adjustment for classical risk factors such as diabetes mellitus, dyslipidemia, hypertension and others [3-5]. Furthermore, the level of high density lipoprotein cholesterol (HDL-C) in plasma is a major determinant of susceptibility to coronary atherosclerosis [6-8]. Genetic studies have recognized the impact of genetic mutations on plasma HDL-C
The Thom Class and Localization of SUSY QM Generating Functional
O. Vana
Physics , 2001,
Abstract: We demonstrate the usage of explicit form of the Thom class found by Mathai and Quillen for the definition of generating functional of a simple supersymmetric quantum mechanical model.
Multiblock Copolymers of Styrene and Butyl Acrylate via Polytrithiocarbonate-Mediated RAFT Polymerization
Bastian Ebeling,Philipp Vana
Polymers , 2011, DOI: 10.3390/polym3020719
Abstract: When linear polytrithiocarbonates as Reversible Addition-Fragmentation chain Transfer (RAFT) agents are employed in a radical polymerization, the resulting macromolecules consist of several homogeneous polymer blocks, interconnected by the functional groups of the respective RAFT agent. Via a second polymerization with another monomer, multiblock copolymers—polymers with alternating segments of both monomers—can be prepared. This strategy was examined mechanistically in detail based on subsequent RAFT polymerizations of styrene and butyl acrylate. Size-exclusion chromatography (SEC) of these polymers showed that the examined method yields low-disperse products. In some cases, resolved peaks for molecules with different numbers of blocks (polymer chains separated by the trithiocarbonate groups) could be observed. Cleavage of the polymers at the trithiocarbonate groups and SEC analysis of the products showed that the blocks in the middle of the polymers are longer than those at the ends and that the number of blocks corresponds to the number of functional groups in the initial RAFT agent. Furthermore, the produced multiblock copolymers were analyzed via differential scanning calorimetry (DSC). This work underlines that the examined methodology is very well suited for the synthesis of well-defined multiblock copolymers.
Assessing the impact of biomedical research in academic institutions of disparate sizes
Vana Sypsa, Angelos Hatzakis
BMC Medical Research Methodology , 2009, DOI: 10.1186/1471-2288-9-33
Abstract: The Modified Impact Index (MII) was defined as the ratio of the observed h-index (h) of an institution over the h-index anticipated for that institution on average, given the number of publications (N) it produces i.e. (α and β denote the intercept and the slope, respectively, of the line describing the dependence of the h-index on the number of publications in log10 scale). MII values higher than 1 indicate that an institution performs better than the average, in terms of its h-index. Data on scientific papers published during 2002–2006 and within 36 medical fields for 219 Academic Medical Institutions from 16 European countries were used to estimate α and β and to calculate the MII of their total and field-specific production.From our biomedical research data, the slope β governing the dependence of h-index on the number of publications in biomedical research was found to be similar to that estimated in other disciplines (≈0.4). The MII was positively associated with the average number of citations/publication (r = 0.653, p < 0.001), the h-index (r = 0.213, p = 0.002), the number of publications with ≥ 100 citations (r = 0.211, p = 0.004) but not with the number of publications (r = -0.020, p = 0.765). It was the most highly associated indicator with the share of country-specific government budget appropriations or outlays for research and development as % of GDP in 2004 (r = 0.229) followed by the average number of citations/publication (r = 0.153) whereas the corresponding correlation coefficient for the h-index was close to 0 (r = 0.029). MII was calculated for first 10 top-ranked European universities in life sciences and biomedicine, as provided by Times Higher Education ranking system, and their total and field-specific performance was compared.The MII should complement the use of h-index when comparing the research output of institutions of disparate sizes. It has a conceptual interpretation and, with the data provided here, can be computed for the total re
3. A comparison between serum inhibin A levels in pre-eclampsia and normotensive pregnancy
Harshini Vana,Suchitra Thunga
International Journal of Pharmaceutical and Biomedical Research (IJPBR) , 2012,
Abstract: Aim: To evaluate the validity of serum inhibin A levels in assessing the severity of preeclampsia. Methods: Hospital based, prospective study conducted in Kasturba Medical College, Mangalore. Inclusion criteria: The patients in the study will be divided into cases and controls based on the criteria proposed. 5mL of venous blood was collected from each patient in an aseptic plain and EDTA vacutainer. Serum inhibin A levels estimated by the ELISA method. Statistical analysis: The samples were statistically analysed by [chi-square] χ2, student’s t-test. Mann-Whitney U-test was used for the comparison of multiples of median. All tests were two‐sided.Statistical significance was inferred for P values <0.05. Results: 72 patients were studied. 36 women were preclampsia cases and 36 were controls, were also divided in to subgroups mild and severe preeclampsia. The median value of inhibin A level in normotensive controls was 901.4pg/mL while in mild pre-eclampsia patients was 1125pg/mL (p>0.05), which was statistically not significant while median serum inhibin A level in severe pre-eclampsia was 1472.5pg/mL (p <0.05), which was statistically significant. Conclusion: There is increase in the serum inhibinA levels in pre-eclampsia group when compared to that in normotensive women. The severe pre-eclamptic sub group patients had statistically significant rise of MOM [p<0.05] compared to that of normotensive controls, there is considerable overlapping of serum inhibin A values among cases and controls. Further studies are needed to prove whether serum inhibinA levels can be used for prediction of pre-eclampsia in India.
Differential cross section for Aharonov--Bohm effect with non standard boundary conditions
P. Stovicek,O. Vana
Physics , 1998, DOI: 10.1209/epl/i1998-00486-2
Abstract: A basic analysis is provided for the differential cross section characterizing Aharonov--Bohm effect with non standard (non regular) boundary conditions imposed on a wave function at the potential barrier. If compared with the standard case two new features can occur: a violation of rotational symmetry and a more significant backward scattering.
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