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Search Results: 1 - 10 of 87791 matches for " VINTIL? I. "
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STABILITY IN REAL TIME OF SOME CRYOPRESERVED MICROBIAL STRAINS WITH REFERENCE TO GENETICALLY MODIFIED MICROORGANISMS
VINTIL? DANIELA,VINTIL? T.,POPESCU I.
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: The aim of this work is to analyze the viability of microorganisms from Collection ofIndustrial Microorganisms from Faculty of Animal Science and Biotechnology –Timisoara, during freezing and thawing as part of cryopreservation technique. Thestability in real time of 19 strains cryopreserved in 16% glycerol was evaluatedduring a 6-months period. The strains studied were: Escherichia coli, Lactobacillusacidophilus, Rhizobium meliloti, Saccharomyces cerevisiae, Aspergillus oryzae,Aspergillus niger, Trichoderma viride, Bacillus globigii, Bacillus licheniformis, and9 strains of Bacillus subtilis. The strains cryopreserved at -20oC and -70oC wereactivated using the fast thawing protocol. A better cell recovery was achieved withthe -70oC protocol reaching an average viability for E. coli of 86,3%, comparingwith 78,6% in -20oC protocol. The cell recovery percentages for the other strainswere: 92,4% for L. acidophilus, 93,9% for A.niger, 89% for A. oryzae, 86,7% for T.viride, 94,2% for R. meliloti, 82,1% for S. cerevisiae, 89,9% for B. licheniformis.Regarding the viability of genetically modified microorganisms, the values shows agood recovering after freezing and thawing, even after 180 days of cryopreservation.With the -20oC protocol lower viability was observed due probably to the formationof eutectic mixtures and re-crystalization processes
EFFECT OF CZB CULTURE MEDIA ON IN VITRO DEVELOPMENT OF CLONED MOUSE EMBRYOS
CARAB? V.,VINTILI.,TELEA ADA,IVAN ALEXANDRA
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: The effect of simple and sequential embryo culture media on the preimplantationdevelopment of mouse nuclear transfer (NT) embryos reconstructed with cumuluscell nuclei using a mechanical NT technique was studied. Blastocyst formation ratewas evaluated using CZB medium. Nonmanipulated and sham-manipulatedparthenogenetic embryos served as controls for, respectively, the medium and thehandling technique. Rates of blastocyst formation for medium and handling controlembryos were similar in CZB (50% and 53%). Development of NT embryos wassignificantly impaired from the two-cell stage onwards, reaching the blastocyst stage ata rate of 4% in CZB. These data demonstrate not only that NT embryos are moresensitive to in vitro culture conditions than parthenogenetic control embryos but alsothat selection of culture media can influence the preimplantation development of NTembryos.
RESEARCHES REGARDING THE INFLUENCE OF THE NUMBER OF CUMULAR CELLS LAYER OVER THE OOCYTE MATURATION EFFICIENCY
V. CARAB?,I. VINTIL,ALEXANDRA IVAN,ADA (CEAN) TELEA
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2009,
Abstract: During the experiments we have carried out with imature oocyte collected from the ovarian follicles, wefound a variety of oocyte-cumulus complexes. We got the following experiment in order to understand therole of cumular cells on the achievement of the cytoplasma and oocyte nucleus maturation. We select theoocyte-cumulus complexes collected both from cows and sows according to the number of cumular celllayers and we watched their development to the blastocyst stade. Thus, we achieved three groups of COC(oocyte-cumulus complexes).One group was made of oocyte without cumular cells, the second group had a layer of cumular cells andthe third group had many layers of cumular cells. we performed an incubation of all these types of COCin TCM-199 enriched with 20% of bovine fetal serum. Because only 1,2 oocyte of the ones who lack thecumular cells layer had maturation signs during cultivation in the thermostat versus 55 and 115,respectively, of the ones that had many cellular layers, presents a solid evidence that cumular cells areindispensable for the maturation and even to the fecundation process. The cumular cells perform adecisive role on the cytoplasma and oocyte nucleus maturation process.
CONDITIONING MICROBIAL PRODUCTS CONTAINING NITROGEN FIXING BACTERIA WITH DIFFERENT SOLID EXCIPIENTS
VINTIL? DANIELA,VINTIL? T.
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: The stability in real time of two strains of Rhizobium (Rhizobium meliloti andRhizobium japonicum) mixed with different excipients was evaluated during a6-months period. The excipients studied were: peat, peat and calciumcarbonate, zeolite, and ceramic. Liquid cultures and excipients mixtures weredried (12-14% humidity), sealed in plastic bags and preserved at +4oC. Thecells were activated periodically by suspending aliquots from dry products in0.9% saline solution. The viability of Rhizobium cells was evaluated bycultivation of diluted suspensions in YMA plates. The number of viable cells isdecreasing during drying in all cases, increase in the first month of storage,and remains constant or decrease very slowly during storage for all obtaineddry products containing rhizobia mixed with solid dry excipients. The highestnumber of viable cells at the end of the experiment was obtained in ceramicwith Rhizobium japonicum (8x105 cells/gram), and the lowest number ofviable cells was obtained in zeolite with Rhizobium meliloti (1,1x103cells/gram).
STUDY REGARDING EFFICIENCY OF INDUCED GENETIC TRANSFORMATION IN BACILLUS LICHENIFORMIS WITH PLASMID DNA
VINTIL? T.
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: A strain of Bacillus licheniformis was subject to genetic transformation with plasmidvectors (pLC1 and pNC61), using electroporation technique, protoplasttransformation and bivalent cations (CaCl2) mediated transformation. In the case oftransformation by electroporation of Bacillus licheniformis B40, the highest numberof transformed colonies (3) were obtained only after a 1,79 KV electric shock, for 2,2milliseconds. Using this transformation technique we have obtained six kanamycinresistant transformants. The frequency of Bacillus licheniformis B40 protoplaststransformation using pLC1 and pNC61 plasmid vectors is approximately 10% (TF =10%). As a result of pLC1 plasmid integration in Bacillus licheniformis protoplasts,six kanamycin resistant transformants were obtained. The pNC61 plasmid, whichconfers trimethoprim resistance, does not integrate in receiver cells by protoplasttransformation. The direct genetic transformation in the presence of bivalent cations(CaCl2), mediated by pLC1 and pNC61 plasmid vectors, produce a lowtransformation frequency. Using this technique, we have obtained three trimethoprimresistant colonies and four kanamycin resistant colonies. The chemical way oftransformation is the only technique, which realizes the integration of pNC61 in B.licheniformis B40 cells.
A NEW DIMENSION INCLUDED IN TEACHING EDUCATIONAL SCIENCES: HEALTHY EATING BEHAVIOUR
MONA VINTIL
Anale : Seria ?tiin?e Economice. Timi?oara , 2012,
Abstract: Quality in higher education includes constant interest to adapt our curricula and educational offer to the needs of the students. In recent years an increased interest is given to healthy nutrition, healthy eating behaviour. The persons required to start working with this topic are the personnel of nurseries, crèches, and kindergartens. They should acquire this knowledge during their university studies. Unfortunately, as we all know, our curriculum is already overloaded so very little is taught about this topic. This makes professionals feel unprepared to teach this subject to the children, as they lack knowledge. We have done a research in the kindergartens of Timisoara and also among the students of the Educational Sciences department to assess the situation and the needs in this area. In cooperation with 4 other universities we have conceived a best practice guide to help professionals deal with this problem.
Ecological footprint evaluation of improved student’s menus using fishery products
Iuliana Vintil
Aquaculture, Aquarium, Conservation & Legislation , 2010,
Abstract: The ecological footprints (EF) analyses were conducted in order to evaluate the environmentimpact of improving actual catering system by replacing 50-100% of red meat products (beef) withlocal/regional fishy products in the weekly meals created for “Dunarea de Jos” University (UGAL)students in 2010. Product-specific EF was calculated from consistent and quality-controlled life cycleinformation of food products and services, including energy, materials, transport, waste treatment andinfrastructural processes. The reducing of red meat products in the student’s daily menus with 50% andthe reducing of long food chain at the local/regional level determined a 36.24% average decreasing ofactual menu EF and the replacing of red meat with fishy products a 72.2% reducing of Eco-menus EF. Atleast 20.83% less amount of money could be saved in the menu creations and if we replace one day in aweek 50% meat with local fishy products and the average reducing EF for menu creation in an academicyear could be in average 17.27%.
The Management of the Citizen Oriented Applications
Ion IVAN,Bogdan VINTIL
Theoretical and Applied Economics , 2010,
Abstract: The context of the knowledge based society is presented. The new user requirements in the context of the new society are analyzed. Basic concepts regarding the citizen oriented applications are presented. Issues specific to the citizen oriented applications are presented. The development cycle of the citizen oriented applications is analyzed. The particular elements for developing citizen oriented applications are described. The quality concept for the citizen oriented applications is defined. Quality characteristics and the costs of quality are defined and analyzed. A system of indicators for the quantification of the quality of the citizen oriented applications is developed. Ways of increasing the quality of the applications are analyzed. Issues as improving the users’ training level, implementing new development techniques, advanced testing techniques and the requirement of audit are approached. The concept of optimization is defined. Optimum criteria are defined and analyzed. Ways of optimizing applications are described. Security requirements are enumerated and described. The particularities of the security requirements for the citizen oriented applications are analyzed. Measures for ensuring the security of the citizen oriented applications are described. A citizen oriented application for the analysis of the structured entities is developed. The application collects data regarding the behavior of the users. The collected data are used for verifying the hypotheses regarding the quality characteristics if the citizen oriented informatics applications.
Substantiating the Incurred but not Reported Reserve
Georgeta Vintil,Daniel Armeanu
Theoretical and Applied Economics , 2009,
Abstract: In order to handle past and future liability taken by insurance contracts concluded, any insurance company must constitute and maintain technical reserves. Substantiating technical reserves is done through actuarial methods and its over-evaluation or under-evaluation influence solvency and financial performance of the insurance companies, in the sense of reducing solvency through over-evaluating reserves and, respectively, influencing profit (hence of outstanding tax) through under-evaluating reserves. An important reserve for insurance companies is represented by the incurred but not reported reserve, as it allows the estimation of the liability the company may confront in the future, generated by events occurred in the past, which are not currently known in the present but will be reported in the future.
ALEXANDRU-OVIDIU VINTIL , Traian Br ileanu, under the Mark of the Categorical Imperative (a Biobibliographical Approach)
ALEXANDRU-OVIDIU VINTIL
Revista Roman? de Sociologie , 2010,
Abstract: This study proposes a biobibliographical approach on Traian Br ileanu, one of the most interesting theoreticians of the elites, one of the most important European sociologists of the nations who had a systemic vision about society, the first Romanian author of a work dealing with the science of politics and one of the most prolific translators of Aristotle and Kant. In fact, his entire work was influenced by the Kantian doctrine. His work is very complex and resisted in spite of the interdictions of the communist regime. This is why we will try to reconsider and re-evaluate it in order to give Traian Br ileanu the place he deserves.
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