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Search Results: 1 - 10 of 496351 matches for " Víctor M; Denova-Gutiérrez "
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A workplace physical activity program at a public university in Mexico can reduce medical costs associated with type 2 diabetes and hypertension
Méndez-Hernández,Pablo; Dosamantes-Carrasco,Darina; Siani,Carole; Flores,Yvonne N; Arredondo,Armando; Lumbreras-Delgado,Irma; Granados-García,Víctor M; Denova-Gutiérrez,Edgar; Gallegos-Carrillo,Katia; Salmerón,Jorge;
Salud Pública de México , 2012, DOI: 10.1590/S0036-36342012000100004
Abstract: objective: to assess the impact of a workplace leisure physical activity program on healthcare expenditures for type 2 diabetes and hypertension treatment. material and methods: we assessed a workplace program's potential to reduce costs by multiplying the annual healthcare costs of patients with type 2 diabetes and hypertension by the population attributable risk fraction of non-recommended physical activity levels. feasibility of a physical activity program was assessed among 425 employees of a public university in mexico. results: if 400 sedentary employees engaged in a physical activity program to decrease their risk of diabetes and hypertension, the potential annual healthcare cost reduction would be 138 880 us dollars. each dollar invested in physical activity could reduce treatment costs of both diseases by 5.3 dollars. conclusions: this research meets the call to use health economics methods to re-appraise health priorities, and devise strategies for optimal allocation of financial resources in the health sector.
Scale for assessing the quality of Mexican adults' mealtime habits
Dosamantes-Carrasco,Darina; Méndez-Hernández,Pablo; Denova-Gutiérrez,Edgar; Lamure,Michel; Morales,Leo; Talavera,Juan O; Espinosa,Patricia; Salmerón,Jorge;
Salud Pública de México , 2011, DOI: 10.1590/S0036-36342011000200008
Abstract: objective: to construct a scale for assessing the quality of mealtime habits in a sample of urban mexican adults, computing the contribution of a set of advisable and unadvisable mealtime habits. material and methods: we performed an exploratory factor analysis among 7 472 adults participating in the baseline assessment of the health workers cohort study, to assess the mealtime habits quality. likelihood ratio test for difference of two probabilities and test for the difference of two means were used to identify differences between low and high categories of the mealtime habits quality scale (mhqs) across variables of interest. results: participants with the top quality of mealtime habits showed lower rates of overweight, obesity, abdominal obesity, and elevated body fat. they were also more adherent to a prudent dietary pattern than a western dietary pattern, and consumed more fruits and vegetables. conclusions: anthropometric and dietary variables differed across mhqs categories. however, further validation of the scale, and assessment of their ability to predict weight gain or related diseases are needed, using prospective and intervention studies.
Conductas de riesgo en una muestra de adolescentes chilenos y mexicanos: un estudio comparativo
Gerardo HUITRóN-BRAVO,Edgar DENOVA-GUTIéRREZ,Elizabeth HALLEY-CASTILLO,Sylvia SANTANDER-RIGOLLET
Papeles de población , 2011,
Abstract: Los adolescentes son una población cuya morbilidad y mortalidad se relacionan estrechamente con determinados factores y conductas de riesgo. Por otro lado, la familia y los estilos de vida saludables son factores que se relacionan con una disminución de las conductas de riesgo en este grupo de edad. El objetivo de este ensayo es determinar las principales conductas de riesgo en una muestra de adolescentes chilenos y mexicanos. Se realizó un estudio transversal, utilizando datos de 1 568 adolescentes entre 10 y 21 a os de edad, de los cuales 815 fueron chilenos de la ciudad de Santiago y 753 fueron mexicanos de la ciudad de Toluca, todos estudiantes activos de sus colegios. La información de interés se recabó utilizando un cuestionario auto-aplicado. Los resultados apuntan a que en los adolescentes chilenos y mexicanos la prevalencia de consumo de sustancias lícitas fue la siguientes: tabaco (57.5 y 38.7 por ciento); alcohol (64.3 y 54.1 por ciento) respectivamente. Encontramos que el tipo de familia que predomina es la biparental, siendo mayor esta forma de estructura en las familias de adolescentes mexicanos (78.9 por ciento) que en los chilenos (55.5 por ciento). Además, se observó que el riesgo de tener relaciones sexuales a edades tempranas es 1.7 veces mayor en adolescentes provenientes de familias disfuncionales. Con base en estos hallazgos concluimos que existe una alta prevalencia de factores y conductas de riesgo en adolescentes chilenos ymexicanos. Nuestros datos sugieren, que los adolescentes que provienen de familias percibidas como disfuncionales, tienen una mayor probabilidad de desarrollar ciertas conductas de riesgo como por ejemplo el consumo de sustancias licitas e inicio temprano de la actividad sexual.
Dietary Glycemic Index, Dietary Glycemic Load, Blood Lipids, and Coronary Heart Disease
Edgar Denova-Gutiérrez,Gerardo Huitrón-Bravo,Juan O. Talavera,Susana Casta ón,Katia Gallegos-Carrillo,Yvonne Flores,Jorge Salmerón
Journal of Nutrition and Metabolism , 2010, DOI: 10.1155/2010/170680
Abstract: Objective. To examine the associations of dietary glycemic index (GI) and dietary glycemic load (GL) with blood lipid concentrations and coronary heart disease (CHD) in nondiabetic participants in the Health Worker Cohort Study (HWCS). Materials and Methods. A cross-sectional analysis was performed, using data from adults who participated in the HWCS baseline assessment. We collected information on participants' socio-demographic conditions, dietary patterns and physical activity via self-administered questionnaires. Dietary GI and dietary GL were measured using a validated food frequency questionnaire. Anthropometric and clinical measurements were assessed with standardized procedures. CHD risk was estimated according to the sex-specific Framingham prediction algorithms. Results. IIn the 5,830 individuals aged 20 to 70 who were evaluated, dietary GI and GL were significantly associated with HDL-C, LDL-C, LDL-C/HDL-C ratio, and triglycerides serum levels. Subjects with high dietary GI have a relative risk of 1.56 (CI 95%; 1.13–2.14), and those with high dietary GL have a relative risk of 2.64 (CI 95%; 1.15–6.58) of having an elevated CHD risk than those who had low dietary GI and GL. Conclusions. Our results suggest that high dietary GI and dietary GL could have an unfavorable effect on serum lipid levels, which are in turn associated with a higher CHD risk.
Dietary Glycemic Index, Dietary Glycemic Load, Blood Lipids, and Coronary Heart Disease
Edgar Denova-Gutiérrez,Gerardo Huitrón-Bravo,Juan O. Talavera,Susana Casta?ón,Katia Gallegos-Carrillo,Yvonne Flores,Jorge Salmerón
Journal of Nutrition and Metabolism , 2010, DOI: 10.1155/2010/170680
Abstract: Objective. To examine the associations of dietary glycemic index (GI) and dietary glycemic load (GL) with blood lipid concentrations and coronary heart disease (CHD) in nondiabetic participants in the Health Worker Cohort Study (HWCS). Materials and Methods. A cross-sectional analysis was performed, using data from adults who participated in the HWCS baseline assessment. We collected information on participants' socio-demographic conditions, dietary patterns and physical activity via self-administered questionnaires. Dietary GI and dietary GL were measured using a validated food frequency questionnaire. Anthropometric and clinical measurements were assessed with standardized procedures. CHD risk was estimated according to the sex-specific Framingham prediction algorithms. Results. IIn the 5,830 individuals aged 20 to 70 who were evaluated, dietary GI and GL were significantly associated with HDL-C, LDL-C, LDL-C/HDL-C ratio, and triglycerides serum levels. Subjects with high dietary GI have a relative risk of 1.56 (CI 95%; 1.13–2.14), and those with high dietary GL have a relative risk of 2.64 (CI 95%; 1.15–6.58) of having an elevated CHD risk than those who had low dietary GI and GL. Conclusions. Our results suggest that high dietary GI and dietary GL could have an unfavorable effect on serum lipid levels, which are in turn associated with a higher CHD risk. 1. Introduction Coronary Heart Disease (CHD) is a major public health problem and the leading cause of death in Mexico [1]. Considerable epidemiological evidence shows that lifestyle, especially diet, influences occurrence of CHD. The role that dietary carbohydrates play in CHD risk has recently received particular attention [2, 3]. High carbohydrate intake has been found to have an adverse effect on serum lipid levels and glucose metabolism, which are likely to increase the risk of CHD [4–6]. Glycemic index (GI) and glycemic load (GL) have been used to quantify the glycemic burden of carbohydrates in particular foods. The GI is the average propensity of carbohydrates in the diet to increase blood glucose compared with a reference food [7]. The GL is defined as the product of GI and the carbohydrate content, reflecting both a food’s glycemic index and carbohydrate levels [8, 9]. Both dietary GI and GL appear to have increased in recent years because of increasing carbohydrate intake and changes in food processing [10]. Diets with a high GI and GL are positively associated with CHD [11], and type 2 diabetes [8, 9], probably in part because they have adverse effects on blood lipid levels; some
Conductas de riesgo en una muestra de adolescentes chilenos y mexicanos: un estudio comparativo
Huitrón-Bravo, Gerardo;Denova-Gutiérrez, Edgar;Halley-Castillo, Elizabeth;Santander-Rigollet, Sylvia;Bórquez-Puga, Mariana;Zapata-Pérez, Luis;Huinca, Bernardo;Zubarew, Tamara G.;Villarroel-del-Pino, Luis;
Papeles de población , 2011,
Abstract: los adolescentes son una población cuya morbilidad y mortalidad se relacionan estrechamente con determinados factores y conductas de riesgo. por otro lado, la familia y los estilos de vida saludables son factores que se relacionan con una disminución de las conductas de riesgo en este grupo de edad. el objetivo de este ensayo es determinar las principales conductas de riesgo en una muestra de adolescentes chilenos y mexicanos. se realizó un estudio transversal, utilizando datos de 1 568 adolescentes entre 10 y 21 a?os de edad, de los cuales 815 fueron chilenos de la ciudad de santiago y 753 fueron mexicanos de la ciudad de toluca, todos estudiantes activos de sus colegios. la información de interés se recabó utilizando un cuestionario auto-aplicado. los resultados apuntan a que en los adolescentes chilenos y mexicanos la prevalencia de consumo de sustancias lícitas fue la siguientes: tabaco (57.5 y 38.7 por ciento); alcohol (64.3 y 54.1 por ciento) respectivamente. encontramos que el tipo de familia que predomina es la biparental, siendo mayor esta forma de estructura en las familias de adolescentes mexicanos (78.9 por ciento) que en los chilenos (55.5 por ciento). además, se observó que el riesgo de tener relaciones sexuales a edades tempranas es 1.7 veces mayor en adolescentes provenientes de familias disfuncionales. con base en estos hallazgos concluimos que existe una alta prevalencia de factores y conductas de riesgo en adolescentes chilenos y mexicanos. nuestros datos sugieren, que los adolescentes que provienen de familias percibidas como disfuncionales, tienen una mayor probabilidad de desarrollar ciertas conductas de riesgo como por ejemplo el consumo de sustancias licitas e inicio temprano de la actividad sexual.
Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
de los Reyes,Mónica; Palomino,Jaime; Martínez,Víctor; Aretio,Carolina; Gutiérrez,Michel;
Biological Research , 2011, DOI: 10.4067/S0716-97602011000200005
Abstract: we evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. each sperm sample was incubated in canine capacitation medium (ccm) for 0, 1, 2 and 3 h at 20°c and at 37°c. after incubation, the samples were assessed by the indirect immunofluorescent staining technique. the probability of having unlabeled sperm (pus), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. there was a linear relationship between pus and time at both temperatures (p<0.001); however, a major percentage of unlabeled sperm was observed in frozen/thawed samples soon after incubation, indicating that the release of acrosin was affected by capacitation time, mainly in frozen samples. temperature influenced acrosin release only in cryopreserved sperm (p<0.05). acrosin activity was measured by digestion halos on slides coated with gelatin-substrate film during each time period; a significant increase in the number of large halos was observed in fresh samples throughout the experiment, whereas frozen/thawed sperm showed a decreased rate of halo diameters during culture. thus, there appears to differences between fresh and frozen dog sperm in terms of acrosin release and the level of acrosin activity in the course of in vitro capacitation.
ELISA para la Identificación de los Patrones Alimentarios de Triatominae en Colombia
Farfán,Ana E.; Gutiérrez,Reinaldo; Angulo,Víctor M.;
Revista de Salud Pública , 2007, DOI: 10.1590/S0124-00642007000400013
Abstract: objective standardising the elisa technique for identifying triatomine insects' feeding behaviour. methods the elisa test was standardised by preparing 12 animal anti-specie polyclonal antisera by immunising rabbits with sera from domestic and wild animals; the sera were titred and absorbed to improve specificity. the intestinal content of fifth-instar rhodnius prolixus (previously fed on each host) was used as positive control; negative controls were obtained from triatomines without feeding. the intestinal content from 60 intradomicile r. prolixus collected in the field was processed to determine the test's effectiveness. results the high-reactivity elisa technique was standardised in detecting every blood protein in the positive controls used here. blood proteins from one or more domestic and wild hosts were detected in 70% of the intestinal content of triatomines collected in homes. bird, human, pig and goat blood were the most frequent feeding sources; blood proteins from wild animals were identified in 9.5% of them. conclusions the technique was shown to be effective in detecting blood proteins from different hosts in the intestinal content of triatomines taken from the laboratory and the field. even though domestic animals' blood was preferentially determined, the findings from wild animals' blood could indicate insect mobility probably from the wild to the domicile. this tool helps in understanding triatomines' behaviour regarding their hosts, thereby contributing to understanding chagas' disease eco-epidemiology.
Detección de la expresión génica in vivo de Mycobacterium tuberculosis durante la tuberculosis pulmonar activa Mycobacterium tuberculosis in vivo-expressed genes detection during active pulmonary tuberculosis
Alejandra Otazo M,Ingrid Gutiérrez S,Víctor Acevedo F,Carlos Calderón A
Revista Chilena de Enfermedades Respiratorias , 2012,
Abstract: El estudio de la expresión génica de Mycobacterium tuberculosis ha involucrado la experimentación "in vitro ", "ex vivo " e "in vivo " (modelos animales), pero aún sin el éxito esperado. Proponemos que revelar los factores clave de la tuberculosis humana requiere investigar la expresión génica de M. tuberculosis dentro del ser humano ("in vivo "). Para ello, aislamos el mRNA total de M. tuberculosis, desde muestras clínicas respiratorias de pacientes con diagnóstico de tuberculosis pulmonar; posteriormente, sintetizamos el dscDNA y lo analizamos mediante RT-PCR cualitativo. Detectamos la expresión de la secuencia de inserción IS6110 y de los genes "housekeeping " 16SrRNA y sigA en M. tuberculosis creciendo in vivo (tuberculosis pulmonar) así como cultivado in vitro. La expresión de los genes mprA y mprB, que codifican el sistema de transducción de se ales MprAB, sólo se detectó en M. tuberculosis crecido in vitro. Con nuestros resultados damos el primer paso hacia la implementación de un método no invasivo para el estudio del transcriptoma de M. tuberculosis, dentro de su único hospedero natural, con el fin de analizar la regulación "in vivo" de los determinantes genéticos requeridos para su virulencia y patogénesis. Mycobacterium tuberculosis gene expression studies have involved "in vitro", "ex vivo" and "in vivo" experiments (animal models), but without the expected success. We propose that key features of human tuberculosis could be discovered by studying the M. tuberculosis gene expression within the human host. Therefore, we isolated totalM. tuberculosis mRNA from human clinical respiratory specimens of patients diagnosed with pulmonary tuberculosis; after this, we synthesized the dscDNA and tested it by qualitative RT-PCR assays. We detected the expression of IS6110 insertion sequence and of the "housekeeping" genes 16SrRNA andsigA in M. tuberculosis grown in vivo (pulmonary tuberculosis) as well as grown in vitro M. tuberculosis. mprA and mprB genes expression, which code the MprAB signal transduction system, were only detected in M. tuberculosis grown in vitro. Our results provide the first step towards a non invasive methodfor the study of the transcriptome of M. tuberculosis within its native host, to analyze "in vivo" regulation of the genetic determinants required for virulence and pathogenesis.
Presence of Risk Factors and Co-Factors for Acquiring HPV Infection or Cervical Cancer in Women Living in a Rural Area and an Urban  [PDF]
Terán-Figueroa Yolanda, Canúas-Landero Víctor Gabriel, Ramírez-Flores élfego, Hernández-Madrigal Mónica, Gallegos-García Verónica, Gutiérrez-Enríquez Sandra Olimpia
Health (Health) , 2014, DOI: 10.4236/health.2014.68088
Abstract: Objective: Identify the presence of some risk factors and co-factors for acquiring HPV (human papilloma virus) or cervical cancer in women from a rural area and an urban area. Material and Methods: Cross-sectional study conducted in 2008 in the State of San Luis Potosí, México. Sampling of non-probabilistic convenience was applied. 99 women participated (73 from urban area and 26 rural). Two surveys were used to identify risk factors and socio-demographic data. All women underwent HPV testing; the samples were self-collected and processed by the Hybrid Capture II method?. To analyze the data, descriptive and inferential statistics were applied. Results: The predominant age range was 37 to 42 years. 38.5% is in a rural community and an urban 37.3%. 72.6% began their sexual life at 19 years or more in urban areas and 57.6% before 18 in rural areas. In relation to the number of sexual partners reported having had only one, 73.1% and 67.1% in rural and urban area respectively. Risk factors that were associated with HPV infection were the beginning of sexual activity at age 18 and under (p = 0.041) and the number of sexual partners (p = 0.001). Conclusions: There are more risk factors in women in urban areas but in rural areas it had the highest percentage of positive cases for infection by high-risk HPV.
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