oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2018 ( 2 )

2017 ( 3 )

2016 ( 1 )

2015 ( 95 )

Custom range...

Search Results: 1 - 10 of 1907 matches for " Uwe Schirkonyer "
All listed articles are free for downloading (OA Articles)
Page 1 /1907
Display every page Item
Affiliation of Dihydrolipoyl Dehydrogenase Allozymes in Mycorrhizae of European Forest Trees and Characterization of the Enzyme of the Matt Bolete (Xerocomus pruinatus) and the Bay Bolete (X. badius)  [PDF]
Uwe Schirkonyer, Gunter M. Rothe
Open Journal of Ecology (OJE) , 2018, DOI: 10.4236/oje.2018.86022
Abstract: Mycorrhizal roots of the deciduous trees European beech (Fagus sylvatica (L.)) and Sessile oak (Quercus petraea (MattuschkaLiebl.)) and the conifers Norway spruce (Picea abies (L.) H. Karst.) and European larch (Larix decidua (Mill.)) associated with the ectomycorrhizal fungi matt bolete (Xerocomus pruinatus (Fries 1835)) or bay bolete (X. badius (Fries 1818)) were analysed with respect to the occurrence of dihydrolipoyl dehydrogenase (EC 1.8.1.4) allozymes. In root tissues of the two deciduous trees, two gene loci could be visualized after cellulose acetate electrophoresis while three loci were expressed in root tissues of the two coniferous species. The two fungal species and further ectomycorrhizal fungi expressed exclusively one dihydrolipoyl dehydrogenase gene. In Xerocomus pruinatus and X. badius, the dihydrolipoyl dehydrogenase gene consists of 1460 bp and 1370 bp, respectively, including five introns each consisting of 52 bp. Their DNA sequences correspond to 70 to 90% to other fungal dihydrolipoyl dehydrogenase genes. One monomer of the dimeric dihydrolipoyl dehydrogenase enzyme consists of 486 (X. pruinatus) or 454 (X.
Ectomycorrhizal diversity at five different tree species in forests of the Taunus Mountains in Central Germany  [PDF]
Uwe Schirkonyer, Christine Bauer, Gunter M. Rothe
Open Journal of Ecology (OJE) , 2013, DOI: 10.4236/oje.2013.31009
Abstract:

Ectomycorrhizal fungi were investigated on five different forest tree species growing in pure stands on the south slope of the Taunus Mountains, which are situated at the northern end of the Rhine rift valley in Central Germany. Mycorrhizal fungi accompanying the genus Xerocomus were identified and their frequencies counted. Using ITS markers, 22 different fungal species were identified down to species level and 6 down to genus level. On European beech (Fagus sylvatica) 16 fungal species and 4 genera were identified and on Sessile oak (Quercus petraea) 16 ectomycorrhizal species and 2 genera were determined. On both deciduous trees we observed exclusively: Cortinarius subsertipes, Genea hispidula, Lactarius quietus, Tylopilus felleus and a Melanogaster genus. On Norway spruce (Picea abies) we identified 13 different mycorrhizal species and 3 different genera, on Silver fir (Abies alba) 12 species and 3 genera, and in association with European larch (Larix decidua) 11 species and 3 genera. On these conifers

e-Health technologies for adult hearing screening
S. Stenfelt,T. Janssen,V. Schirkonyer,F. Grandori
Audiology Research , 2011, DOI: 10.4081/audiores.2011.e14
Abstract: The development of hearing diagnosis methods and hearing screening methods are not isolated phenomena: they are intimately related to changes in the cultural background and to advances in fields of medicine and engineering. In the recent years, there has been a rapid evolution in the development of fast, easy and reliable techniques for lowcost hearing screening initiatives. Since adults and elderly people typically experience a reduced hearing ability in challenging listening situations [e.g., in background noise, in reverberation, or with competing speech (Pichora Fuller & Souza, 2003)], these newly developed screening tests mainly rely on the recognition of speech stimuli in noise, so that the real experienced listening difficulties can be effectively targeted (Killion & Niquette, 2000). New tests based on the recognition of speech in noise are being developed on portable, battery- operated devices (see, for example, Paglialonga et al., 2011), or distributed diffusely using information and communication technologies. The evolutions of e-Health and telemedicine have shifted focus from patients coming to the hearing clinic for hearing health evaluation towards the possibility of evaluating the hearing status remotely at home. So far, two ways of distributing the hearing test have primarily been used: ordinary telephone networks (excluding mobile networks) and the internet. When using the telephone network for hearing screening, the predominantly test is a speech-in-noise test often referred to as the digit triplet test where the subjects hearing status is evaluated as the speech-to-noise threshold for spoken digits. This test is today available in some ten countries in Europe, North America and Australia. The use of internet as testing platform allows several different types of hearing assessment tests such as questionnaires, different types of speech in noise tests, temporal gap detection, sound localization (minimum audible angle), and spectral (un)masking tests. Also, the use of the internet allows audiovisual presentations as well as visual interaction and cues in the tests for a more ecological approach. Even if several new and novel approaches for hearing assessment using the internet are surfacing, the validated tests are based on questionnaires or speech-in-noise. Although the internet allows for a broader flora of pure auditory and audiovisual tests for hearing health assessment, calibration problems such as timing uncertainty, output levels and modes of presentation (speakers or earphones) limits the usability at present.
Towards Quantitative Characterisation of the Small Force Transducer Used in Nanoindentation Instruments  [PDF]
Zhi Li, Uwe Brand
Modern Instrumentation (MI) , 2013, DOI: 10.4236/mi.2013.24009
Abstract:

Quantitative characterization of the mechanical properties of materials in micro-/nano-scale using depth-sensing indentation technique demands high performance of nanoindentation instruments in use. In this paper, the efforts to calibrate the capacitive force transducer of a commercial nanoindentation instrument are presented, where the quasi-static characteristic of the force transducer has been calibrated by a precise compensation balance with a resolution of ~1 nN. To investigate the dynamic response of the transducer, an electrostatic MEMS (Micro-Electro-Mechanical System) based on nano-force transfer standard with nano-Newton (10-9 Newton) resolution and a bandwidth up to 6 kHz have been employed. Preliminary experimental results indicate that 1) the force transducer under calibration has a probing force uncertainty less than 300 nN (1σ) in the calibration range of 1 mN; 2) the transient duration at contact points amounts to 10 seconds; 3) the overshoot of engagement is pre-load dependent.

Drosophila muscleblind Codes for Proteins with One and Two Tandem Zinc Finger Motifs
Uwe Irion
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034248
Abstract: Muscleblind-like proteins, Muscleblind (Mbl) in Drosophila and MBNL1-3 in vertebrates, are regulators of alternative splicing. Human MBNL1 is a key factor in the etiology of myotonic dystrophy (DM), a muscle wasting disease caused by the occurrence of toxic RNA molecules containing CUG/CCUG repeats. MBNL1 binds to these RNAs and is sequestered in nuclear foci preventing it from exerting its normal function, which ultimately leads to mis-spliced mRNAs, a major cause of the disease. Muscleblind-proteins bind to RNAs via N-terminal zinc fingers of the Cys3-His type. These zinc fingers are arranged in one (invertebrates) or two (vertebrates) tandem zinc finger (TZF) motifs with both fingers targeting GC steps in the RNA molecule. Here I show that mbl genes in Drosophila and in other insects also encode proteins with two TZF motifs, highly similar to vertebrate MBNL proteins. In Drosophila the different protein isoforms have overlapping but possibly divergent functions in vivo, evident by their unequal capacities to rescue the splicing defects observed in mbl mutant embryos. In addition, using whole transcriptome analysis, I identified several new splicing targets for Mbl in Drosophila embryos. Two of these novel targets, kkv (krotzkopf-verkehrt, coding for Chitin Synthase 1) and cora (coracle, coding for the Drosophila homolog of Protein 4.1), are not muscle-specific but expressed mainly in epidermal cells, indicating a function for mbl not only in muscles and the nervous system.
Modeling the Risk of Secondary Malignancies after Radiotherapy
Uwe Schneider
Genes , 2011, DOI: 10.3390/genes2041033
Abstract: In developed countries, more than half of all cancer patients receive radiotherapy at some stage in the management of their disease. However, a radiation-induced secondary malignancy can be the price of success if the primary cancer is cured or at least controlled. Therefore, there is increasing concern regarding radiation-related second cancer risks in long-term radiotherapy survivors and a corresponding need to be able to predict cancer risks at high radiation doses. Of particular interest are second cancer risk estimates for new radiation treatment modalities such as intensity modulated radiotherapy, intensity modulated arc-therapy, proton and heavy ion radiotherapy. The long term risks from such modern radiotherapy treatment techniques have not yet been determined and are unlikely to become apparent for many years, due to the long latency time for solid tumor induction. Most information on the dose-response of radiation-induced cancer is derived from data on the A-bomb survivors who were exposed to γ-rays and neutrons. Since, for radiation protection purposes, the dose span of main interest is between zero and one Gy, the analysis of the A-bomb survivors is usually focused on this range. With increasing cure rates, estimates of cancer risk for doses larger than one Gy are becoming more important for radiotherapy patients. Therefore in this review, emphasis was placed on doses relevant for radiotherapy with respect to radiation induced solid cancer. Simple radiation protection models should be used only with extreme care for risk estimates in radiotherapy, since they are developed exclusively for low dose. When applied to scatter radiation, such models can predict only a fraction of observed second malignancies. Better semi-empirical models include the effect of dose fractionation and represent the dose-response relationships more accurately. The involved uncertainties are still huge for most of the organs and tissues. A major reason for this is that the underlying processes of the induction of carcinoma and sarcoma are not well known. Most uncertainties are related to the time patterns of cancer induction, the population specific dependencies and to the organ specific cancer induction rates. For radiotherapy treatment plan optimization these factors are irrelevant, as a treatment plan comparison is performed for a patient of specific age, sex, etc. If a treatment plan is compared relative to another one only the shape of the dose-response curve (the so called risk-equivalent dose) is of importance and errors can be minimized.
Pathophysiology of fluid imbalance
Uwe Kreimeier
Critical Care , 2000, DOI: 10.1186/cc968
Abstract: A discussion of the pathophysiology of fluid imbalance typically focuses on hypovolemia. A relevant clinical scenario is described in the following case report.A laboratory worker was taken to the emergency department with malaise, headache, nausea and vomiting [1]. There was evidence of circulatory failure and infection. The patient's pulse was 114 beats/min, blood pressure 42/20 mmHg and oral temperature 40°C. The pre-load filling pressure was very low, while cardiac index was elevated. Clinical symptoms were consistent with a generalized capillary leak syndrome. The patient received 14.9 l fluid in excess of measured output during the period of hypotension. Inotropic support with dopamine and nor-epinephrine was also provided. The presumptive diagnosis was septic shock.It was discovered 11 h after admission that the patient had self-administered 1 mg Salmonella minnesota endotoxin 2.5 h prior to arrival at the emergency department in an attempt to treat a recently diagnosed tumor. This single, large intravenous dose of endotoxin had provoked the manifestations of septic shock syndrome, including hypotension with high cardiac output, disseminated intravascular coagulation, abnormalities of hepatic and renal function, and pulmonary edema. A dose of 100 mg human monoclonal antibody against the lipid A moiety of bacterial lipopolysaccharide (HA-1A) antibody was administered 23 h after the injection of endotoxin. Norepinephrine infusion was discontinued 50 h after the endotoxin injection. The patient was discharged on the eighth hospital day.Although the precipitating cause of this patient's condition was unusual, hypovolemia necessitating fluid administration is a common occurrence. Nevertheless, a proper consideration of fluid imbalance extends beyond hypovolemia alone. Fluid imbalance indeed encompasses a triad of conditions consisting of hypovolemia, normovolemia with maldistribution of fluid, and hypervolemia. Each of these is now discussed.One frequent cause of
Pyoderma gangrenosum – a review
Uwe Wollina
Orphanet Journal of Rare Diseases , 2007, DOI: 10.1186/1750-1172-2-19
Abstract: The treatment of PG is a challenge. Randomized, double-blinded prospective multicenter trials for PG are not available. The best documented treatments are systemic corticosteroids and ciclosporin A. Combinations of steroids with cytotoxic drugs are used in resistant cases. The combination of steroids with sulfa drugs or immunosuppressants has been used as steroid-sparing modalities. Anti-tumor necrosis alpha therapy in Crohn's disease showed a rapid response of PG. Skin transplants and the application of bioengineered skin is useful in selected cases as a complement to the immunosuppressive treatment. Topical therapy with modern wound dressings is useful to minimize pain and the risk of secondary infections. Despite recent advances in therapy, the prognosis of PG remains unpredictable.Pyoderma gangrenosumPyoderma gangrenosum (PG) is a primarily sterile inflammatory neutrophilic dermatosis. It is characterized by recurrent cutaneous ulcerations with mucopurulent or hemorrhagic exudate. These very painful ulcers present with undermined bluish borders with surrounding erythema. In many cases, PG is associated with inflammatory bowel disease, rheumatic disorder or neoplasia [1-3].Powell et al. [4] suggested a classification of PG into four major clinical types [see Table 1].Accurate epidemiological data on PG are missing. The peak of incidence occurs between the ages of 20 to 50 years with women being more often affected than men [2,6]. Cases in infants and adolescents account for only 4% of PG. PG in elderly people has occasionally been reported [7]. The general incidence has been estimated to be between 3 and 10 per million per year [8].PG occurs most commonly on the lower legs with preference for the pretibial area [Fig. 1 &2]. PG has been reported on other sites of the body as well, including breast, hand, trunk, head and neck, and peristomal skin [Fig. 3 &4]. Extracutaneous manifestations include involvement of upper airway mucosa, eye [9-12], genital mucosa [13],
Combining methods - lack of methodology
Flick, Uwe
Papers on Social Representations , 1992,
Abstract:
Der Souver n
Uwe Jochum
Libreas : Library Ideas , 2009,
Abstract:
Page 1 /1907
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.