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Search Results: 1 - 10 of 136819 matches for " Tzi-Yuan Wang "
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A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
Hsin-Liang Chen, Yo-Chia Chen, Mei-Yeh J Lu, Jui-Jen Chang, Hiaow-Ting C Wang, Tzi-Yuan Wang, Sz-Kai Ruan, Tao-Yuan Wang, Kuo-Yen Hung, Hsing-Yi Cho, Huei-Mien Ke, Wan-Ting Lin, Ming-Che Shih, Wen-Hsiung Li
Biotechnology for Biofuels , 2012, DOI: 10.1186/1754-6834-5-24
Abstract: In this study, a cDNA encoding β-glucosidase was isolated from the buffalo rumen fungus Neocallimastix patriciarum W5 and is named NpaBGS. It has a length of 2,331 bp with an open reading frame coding for a protein of 776 amino acid residues, corresponding to a theoretical molecular mass of 85.1 kDa and isoelectric point of 4.4. Two GH3 catalytic domains were found at the N and C terminals of NpaBGS by sequence analysis. The cDNA was expressed in Pichia pastoris and after protein purification, the enzyme displayed a specific activity of 34.5 U/mg against cellobiose as the substrate. Enzymatic assays showed that NpaBGS was active on short cello-oligosaccharides from various substrates. A weak activity in carboxymethyl cellulose (CMC) digestion indicated that the enzyme might also have the function of an endoglucanase. The optimal activity was detected at 40°C and pH 5?~?6, showing that the enzyme prefers a weak acid condition. Moreover, its activity could be enhanced at 50°C by adding Mg2+ or Mn2+ ions. Interestingly, in simultaneous saccharification and fermentation (SSF) experiments using Saccharomyces cerevisiae BY4741 or Kluyveromyces marxianus KY3 as the fermentation yeast, NpaBGS showed advantages in cell growth, glucose production, and ethanol production over the commercial enzyme Novo 188. Moreover, we showed that the KY3 strain engineered with the NpaNGS gene can utilize 2 % dry napiergrass as the sole carbon source to produce 3.32 mg/ml ethanol when Celluclast 1.5 L was added to the SSF system.Our characterizations of the novel β-glucosidase NpaBGS revealed that it has a preference of weak acidity for optimal yeast fermentation and an optimal temperature of ~40°C. Since NpaBGS performs better than Novo 188 under the living conditions of fermentation yeasts, it has the potential to be a suitable enzyme for SSF.
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
Physicochemical Properties of Glycine-Based Ionic Liquid [QuatGly-OEt][EtOSO3] (2-Ethoxy-1-ethyl-1,1-dimethyl-2-oxoethanaminium ethyl sulfate) and Its Binary Mixtures with Poly(ethylene glycol) (Mw = 200) at Various Temperatures
Tzi-Yi Wu,Bor-Kuan Chen,Lin Hao,Yuan-Chung Lin,H. Paul Wang,Chung-Wen Kuo,I-Wen Sun
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12128750
Abstract: This work includes specific basic characterization of synthesized glycine-based Ionic Liquid (IL) [QuatGly-OEt][EtOSO 3] by NMR, elementary analysis and water content. Thermophysical properties such as density, ρ, viscosity, η, refractive index, n, and conductivity, κ, for the binary mixture of [QuatGly-OEt][EtOSO 3] with poly(ethylene glycol) (PEG) [M w = 200] are measured over the whole composition range. The temperature dependence of density and dynamic viscosity for neat [QuatGly-OEt][EtOSO 3] and its binary mixture can be described by an empirical polynomial equation and by the Vogel-Tammann-Fucher (VTF) equation, respectively. The thermal expansion coefficient of the ILs is ascertained using the experimental density results, and the excess volume expansivity is evaluated. The negative values of excess molar volume for the mixture indicate the ion-dipole interactions and packing between IL and PEG oligomer. The results of binary excess property ( VmE) and deviations (Δη, ? xn, ? Фn, ? xR, and ? ФR) are discussed in terms of molecular interactions and molecular structures in the binary mixture.
Synthesis and Characterization of Organic Dyes Containing Various Donors and Acceptors
Tzi-Yi Wu,Ming-Hsiu Tsao,Fu-Lin Chen,Shyh-Gang Su,Cheng-Wen Chang,Hong-Paul Wang,Yuan-Chung Lin,Wen-Chung Ou-Yang,I-Wen Sun
International Journal of Molecular Sciences , 2010, DOI: 10.3390/ijms11010329
Abstract: New organic dyes comprising carbazole, iminodibenzyl, or phenothiazine moieties, respectively, as the electron donors, and cyanoacetic acid or acrylic acid moieties as the electron acceptors/anchoring groups were synthesized and characterized. The influence of heteroatoms on carbazole, iminodibenzyl and phenothiazine donors, and cyano-substitution on the acid acceptor is evidenced by spectral, electrochemical, photovoltaic experiments, and density functional theory calculations. The phenothiazine dyes show solar-energy-to-electricity conversion efficiency ( η) of 3.46–5.53%, whereas carbazole andiminodibenzyl dyesshow η of 2.43% and 3.49%, respectively.
Synthesis, Characterization and Photovoltaic Properties of Di-Anchoring Organic Dyes
Wu, Tzi-Yi;Tsao, Ming-Hsiu;Su, Shyh-Gang;Wang, H. Paul;Lin, Yuan-Chung;Chen, Fu-Lin;Chang, Cheng-Wen;Sun, I-Wen;
Journal of the Brazilian Chemical Society , 2011, DOI: 10.1590/S0103-50532011000400023
Abstract: three new organic dyes comprising carbazole, iminodibenzyl and phenothiazine moieties, as electron donors and di-anchoring rhodanine rings as the electron acceptors, were synthesized and evaluated for use in dye-sensitized solar cells. a solar cell employing dye-containing phenothiazine as a hole-transporting unit and di-anchoring rhodanine rings as the electron acceptors exhibits a short circuit photocurrent density of 10.6 ma cm-2, an open-circuit voltage of 0.658 v and a fill factor of 0.7, corresponding to an overall conversion efficiency of 4.91% at standard am 1.5 sunlight.
Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”
Jian Sun,Hexiang Wang,Tzi Bun Ng
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/219793
Abstract: A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16 kDa and reduced the activity of trypsin with an IC50 value of about 0.6 M. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-3H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC50 value of 28.8 M and 21.5 M, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100 M.
A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum
Yanrui Li,Guoqing Zhang,Tzi Bun Ng,Hexiang Wang
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/716515
Abstract: A lectin designated as Hericium erinaceum agglutinin (HEA) was isolated from dried fruiting bodies of the mushroom Hericium erinaceum with a chromatographic procedure which entailed DEAE-cellulose, CM-cellulose, Q-Sepharose, and FPLC Superdex 75. Its molecular mass was estimated to be 51 kDa and its N-terminal amino acid sequences was distinctly different from those of other isolated mushroom lectins. The hemagglutinating activity of HEA was inhibited at the minimum concentration of 12.5 mM by inulin. The lectin was stable at pH 1.9–12.1 and at temperatures up to 70°C, but was inhibited by Hg2
Constant Force Feedback Controller Design Using PID-Like Fuzzy Technique for Tapping Mode Atomic Force Microscopes  [PDF]
Yuan-Jay Wang
Intelligent Control and Automation (ICA) , 2013, DOI: 10.4236/ica.2013.43031
Abstract:

A novel constant force feedback mechanism based on fuzzy logic for tapping mode Atomic Force Microscopes (AFM) is proposed in this paper. A mathematical model for characterizing the cantilever-sample interaction subsystem which is nonlinear and contains large uncertainty is first developed. Then, a PID-like fuzzy controller, combing a PD-like fuzzy controller and a PI controller, is designed to regulate the controller efforts and schedule the applied voltage of the Z-axis of the piezoelectric tube scanner to maintain a constant tip-sample interaction force during sample-scanning. Using the PID-like fuzzy controller allows the cantilever tip to track sample surface rapidly and accurately even though the topography of the surface is arbitrary and not given in advance. This rapid tracking response facilitates us to observe samples with high aspect ratio micro structures accurately and quickly. Besides, the overshoot which will result in tip crash in commercial AFMs with a traditional PID controller could be avoided. Additionally, the controller efforts can be intelligently scheduled by using the fuzzy logic. Thus, continuous manual gain-tuning by trial and error such as those in commercial AFMs is alleviated. In final, computer simulations and experimental verifications are provided to demonstrate the effectiveness and confirm the validity of the proposed controller.

Bacterial Lysis of Microalgal Cells  [PDF]
Meng Wang, Wenqiao Yuan
Journal of Sustainable Bioenergy Systems (JSBS) , 2014, DOI: 10.4236/jsbs.2014.44022
Abstract: This short communication reports a pioneering research of using bacteria for simultaneous algal cell disruption and cell wall/membrane utilization. Microalgae are regarded as one of the most promising feedstock that can potentially address the twin challenges of energy security and environmental protection due to their fast growth rate, high lipid content and CO2 biofixation capabilities. However, different from their terrestrial oil crops, the extracellular coverings of algae vary significantly, ranging from multiple layers of elaborate scales to highly mineralized coats to complex cell walls consisting of structural fibrils enmeshed in complex matrices. These strong cellular walls and membranes are resistant to disintegration, which makes lipid extraction from microalgae difficult. A bacteria-assisted algal cell disruption and lipid extraction method was studied here. The bacteria Sagittula stellata showed strong algicidal activity against two microalgae, Nannochloropsis oculata and Dunaliella salina. The algicidal rate reached 64.7% on N. oculata and 52.4% on D. salina in six days. A decrease in chlorophyll-a fluorescence density of both algae and bacteria addition was also observed. After 6-day treatment by S. stellata, hexane-extracted crude lipid contents increased from 32.9% to 45.7% and from 19.6% to 36.4% for N. oculata and D. salina, respectively, when compared with no bacterial addition. The preliminary results concluded that S. stellata was effective in the lysis of microalgal cells for effective lipid recovery.
Sedimentary Petrology Characteristics and Their Implications for Provenance of Flowerpot Basin Jurassic System in Yanqing County, Beijing  [PDF]
Yaxuan Yuan, Hongliang Wang
Journal of Geoscience and Environment Protection (GEP) , 2016, DOI: 10.4236/gep.2016.46006
Abstract: The characteristics of sedimentary petrology are significant in determining geological provenance. By using field outcrop observation and indoor identification of thin sections, the characteristics of Jurassic system Tuchengzi Formation clastic rocks exposed in the Beijing Yanqing “flowerpot” basin, which include rock types, clastic composition and cement type, etc. have been analyzed systematically to identify the provenance direction and tectonic setting and nature of the provenance. Research shows that the sedimentary rocks in this area belong to analluvial fan and braided river sedimentary rock system and include mainly conglomerates, lithic sandstone, and feldspathic lithic sandstone. The main compositions of the conglomerate are dolomite and limestone. Nearly 50% to 70% of the sandstones are mainly polycrystalline quartz; 10% to 15% are feldspar; and 40% to 55% are composed of debris from mainly magmatic and metamorphic rocks. A Dickinson triangular diagram indicates that the provenance type in this area is recycled orogny evolving from clastic recycled orogeny such as a collisional orogenic belt to a transitional recycled orogeny. On the basis of the geographic position and tectonic setting, three types of provenance can be identified in the Jurassic system Tuchengzi Formation. The far source provenances are Archean erathem basement metamorphic rocks in the northern basin and granite or adamellite that intruded rocks of the Late Jurassic epoch of the Yanshanian period in the northeast. The nearby provenance is the Mesoproterozoic erathem Changchengian system and Jixianianian system dolomite in both the east and west.
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