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Search Results: 1 - 10 of 406 matches for " Toxopasma gondii "
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Experimental Infection of Calomys callosus (Rodentia, Cricetidae) by Toxoplasma gondii
Favoreto-Junior, Sílvio;AV Ferro, Eloísa;Clemente, Diogo;Silva, Deise AO;Mineo, José R;
Memórias do Instituto Oswaldo Cruz , 1998, DOI: 10.1590/S0074-02761998000100018
Abstract: calomys callosus, rengger 1830 (rodentia, cricetidae), a wild rodent found in central brazil, was studied to investigate its susceptibility to toxoplasma gondii experimental infection and its humoral immune response against this protozoa. the electrophoretic profile of the serum proteins of c. callosus showed that igg, which shows no affinity to protein a, has higher cross reactivity with rat igg than with igg from other rodents. the susceptibility assay was performed by inoculation groups of animals with various suspensions of t. gondii tachyzoites from 102 to 106 parasites. all animals died between 3 and 9 days after infection and the kinetics of antibody synthesis was determined. basically, they recognized predominantly the immunodominant antigen sag-1 (p30). the immunohistochemistry assays revealed that the liver was the most heavily infected organ, followed by the spleen, lungs, intestine, brain and kidneys. it can be concluded that c. callosus is an excellent experimental model for acute phase of toxoplasma infection
Experimental Infection of Calomys callosus (Rodentia, Cricetidae) by Toxoplasma gondii
Favoreto-Junior Sílvio,AV Ferro Eloísa,Clemente Diogo,Silva Deise AO
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract: Calomys callosus, Rengger 1830 (Rodentia, Cricetidae), a wild rodent found in Central Brazil, was studied to investigate its susceptibility to Toxoplasma gondii experimental infection and its humoral immune response against this protozoa. The electrophoretic profile of the serum proteins of C. callosus showed that IgG, which shows no affinity to Protein A, has higher cross reactivity with rat IgG than with IgG from other rodents. The susceptibility assay was performed by inoculation groups of animals with various suspensions of T. gondii tachyzoites from 102 to 106 parasites. All animals died between 3 and 9 days after infection and the kinetics of antibody synthesis was determined. Basically, they recognized predominantly the immunodominant antigen SAG-1 (P30). The immunohistochemistry assays revealed that the liver was the most heavily infected organ, followed by the spleen, lungs, intestine, brain and kidneys. It can be concluded that C. callosus is an excellent experimental model for acute phase of Toxoplasma infection
Prevalence of human toxoplasmosis in san carlos island, venezuela
Chacín-Bonilla,Leonor; Sánchez-Chávez,Yulaicy; Estévez,Jesús; Larreal,Yraima; Molero,Emelyn;
Interciencia , 2003,
Abstract: a survey of 335 individuals, 1-65 years of age (mean ±sd of 20.8 ±15.7), in 6 communities from the san carlos island, western venezuela, was conducted to study the prevalence of serum antibody to toxoplasma gondii. the indirect hemagglutination test showed an overall infection rate of 49.8% (167 of 335) that ranged 23-64.8% according to the locality. no association between antibody status and age or risk factors was detected. higher antibody rates were found in a windward coast community, and lower rates in a rural sector when compared to 3 other localities. higher geometric mean titers were found in the communities with higher antibody rates. toxoplasmosis is prevalent and widely spread in the area. infection by oocysts from cat feces appears to be the predominant mode of transmission and contaminated drinking water seems to play a role in the transmission.
Otimiza??o da rea??o de polimerase em cadeia para detec??o de Toxoplasma gondii em sangue venoso e placenta de gestantes
Spalding, Silvia Maria;Amendoeira, Maria Regina Reis;Coelho, Janice M.C.;Angel, Sergio O.;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2002, DOI: 10.1590/S1676-24442002000200006
Abstract: the toxoplasma gondii detection in venous blood and placenta of pregnants by polymerase chain reaction may facilitate the diagnosis of congenital toxoplasmosis. we evaluated pregnants with reagent immunoglobulin m (igm) and their children. beyond serological toxoplasma-specific immunoglobulins g (igg), m (igm), a (iga) and igg avidity (meia), we did immunoperoxidasis and mice inoculation. genomic amplification with gene b1 primers and new tgr primers (called abgtg7 c1 and n1) was conducted for each sample. it's necessary to observe that treatment probably is responsible for the infection decrease, that manner, negative results would confirm uteri therapeutic efficiency. pcr was sensitive and specific, it identified one to ten tachyzoites. besides being a fast form of congenital toxoplasmosis diagnosis, it may be used safely and trustly, as an important tool for congenital evaluation.
Altera??es tomográficas cerebrais em crian?as com les?es oculares por toxoplasmose congênita
Melamed, Jacobo;Dornelles, Fábio;Eckert, Gabriela U.;
Jornal de Pediatria , 2001, DOI: 10.1590/S0021-75572001000600010
Abstract: objective: to evaluate the frequency and the types of cerebral ct scan alterations in patients with ocular lesions caused by congenital toxoplasmosis. methods: the patients should fit one of the following criteria: reagent igm serology for toxoplasmosis at birth and mother with positive serology for toxoplasmosis; age less than ten years, with bilateral funduscopic lesions compatible with toxoplasmic retinochoroiditis; or reagent igg serology for toxoplasmosis and mother with positive serology for toxoplasmosis. all patients were submitted to noncontrast cerebral ct scan. results: twenty-two patients participated in this study (44 eyes). thirteen eyes presented macular lesions (29.5%), eight had peripheral lesions (18.2%) and 22 eyes had concomitant macular and peripheral lesions (50%). seventeen patients presented radiological alterations (77.3%). among these, sixteen patients (94.1%) presented brain calcifications, two patients (11.7%) presented ventricular dilation, two patients (11.7%) had hydrocephalus, one had hydranencephaly (5.9%), one presented porencephalic cyst (cerebral hemiatrophy) (5.9%), one had arachnoid cyst (5.9%) and one had mild dilation of ventricles and subarachnoid cistern (5.9%). conclusions: patients diagnosed with congenital toxoplasmosis and ocular lesions should be investigated for concomitant neuroradiologic alterations.
Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, S?o Paulo State, Brazil
Langoni, H.;Modolo, J. R.;Pezerico, S. B.;Silva, R. C.;Castro, A. P. B.;Silva, A. V. da;Padovani, C. R.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2006, DOI: 10.1590/S1678-91992006000100012
Abstract: toxoplasmosis is a zoonosis caused by toxoplasma gondii, a coccidian protozoan of worldwide distribution. the seroprevalence in canine population can be an alternative for measuring t. gondii urban spreading. a total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the department of veterinary hygiene and public health, school of veterinary medicine and animal husbandry (fmvz), s?o paulo state university, unesp, together with the county health authorities, in august 1999. using indirect fluorescent antibody test (ifat) for detecting antibodies anti-t. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. the associations between the serological results and the epidemiological variables were studied. statistically significant differences were not found regarding sex (32.2% male and 34.3% female reactors). dogs without a defined breed showed seropositivity statistically higher than the pedigreed group. the occurrence of infection was considered higher with age.
Veterinary vaccines against Toxoplasma gondii
Innes, Elisabeth A;Bartley, Paul M;Maley, Stephen;Katzer, Frank;Buxton, David;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000200018
Abstract: toxoplasma gondii has a very wide intermediate host range and is thought to be able to infect all warm blooded animals. the parasite causes a spectrum of different diseases and clinical symptoms within the intermediate hosts and following infection most animals develop adaptive humoral and cell-mediated immune responses. the development of protective immunity to t. gondii following natural infection in many host species has led researchers to look at vaccination as a strategy to control disease, parasite multiplication and establishment in animal hosts. a range of different veterinary vaccines are required to help control t. gondii infection which include vaccines to prevent congenital toxoplasmosis, reduce or eliminate tissue cysts in meat producing animals and to prevent oocyst shedding in cats. in this paper we will discuss some of the history, challenges and progress in the development of veterinary vaccines against t. gondii.
Detección de anticuerpos séricos contra Toxoplasma gondii (Nicolle y Manceaux, 1909) en llamas (Lama glama Linneaus, 1758) y alpacas (Lama pacos Linneaus, 1758 ) de Chile
Patitucci,A N; Pérez,M J; Barril,G; Cárcamo,C M; Mu?oz,A;
Archivos de medicina veterinaria , 2006, DOI: 10.4067/S0301-732X2006000200013
Abstract: sera samples from 113 llamas (lama glama) and 127 alpacas (lama pacos) from the ix and v regions, respectively, of chile were tested for toxoplasma gondii antibodies. the modified agglutination test (mat) was used in both species and titers 1:25 were considered diagnostically significant based in previously published data. sera from 49 lamas (43.3%) and 15 alpacas (11.8%) were positive to t. gondii. percentaje seropositivity in serum dilutions of 1:25, 1:50, 1:500 and 1:5000 was 17.6%; 7.9%; 14.1% and 3.5% in lamas and 0%; 2.3%; 0.7% and 8.6% in alpacas, respectively. the rather low prevalence in alpacas may be associated with geographical conditions, management practices or contacts with cats rather than different species susceptibility. as expected, older animals showed higher reactivity of t. gondii than young animals.
Comportamento imunológico e antigênico de cinco amostras de Toxoplasma gondii inoculadas em gatos
Navarro, Italmar Teodorico;Vidotto, Odilon;Silva, Andréa Carlos Bekner da;Mitsuka, Regina;Jankevicius, José Vitor;Shida, Paula Namie;Cortês, José de Angelis;
Ciência Rural , 1998, DOI: 10.1590/S0103-84781998000300017
Abstract: the biology of toxoplasma gondii demonstrate that cats are the complete host responsable for the dissemination of this parasites. two domestic cats were immunised with toxoplasma gondii strains vps (human), liv-iv and liv-v (porcine), cpl (caprine) and cn (feline). live tachyzoites were utilized in intravenous inoculation of 2x107 (first inoculum) and 4x107 (second inoculum - 35 days later), except for strain vps, where one cat died 10 days after the first inoculation and another showed symptoms of acute toxoplasmosis. in all other strains, no clinical signs were detected during 6 months of observation. the antibody response after immunization was monitored by indirect immunofluorescence (if) test by the use of anti-cat igg conjugate. the antibody titers obtained at the 20th day varied from 1:1,024 to 1:4,096 and from 1:1,024 to 1:8,000 at the 40th day. only the vps strain attained titers of 1:16,000 at the 30th day of immunization. homologous and heterologos titers were equivalem without any difference among the strain. when the immune sera were adsorbed with live tachyzoites, a reduction in the antibody titers was demonstrated both in homologous and heterologous levels. these results suggest that although dijferences in virulence for cats are evident among the strains. the surface antigens are commom among the t. gondii strains on the basis of if antibody level. the results also demonstrated that apparently there is no correlation between virulence and serological characteristics of the studied strains of toxoplasma gondii. however the importance of the if test in the laboratorial diagnosis of toxoplasmosis is reinforced.
Estandarización del inmunoensayo ELISA para la detección de IgG anti-Toxoplasma gondii en ratón
Parasitología latinoamericana , 2005, DOI: 10.4067/S0717-77122005000100019
Abstract: we describe a standardization of an enzyme-linked immunosorbent assay (elisa) for detection of specific anti-toxoplasma gondii igg in mice. this assay is important as a tool for strains charac-terization in t. gondii and for diagnosis of human toxoplasmosis. the technique was standardized by testing different conditions (different coating reagents and different amounts of antigen). no significant difference was observed between coating reagents; therefore buffer coating with bovine albumin 1% was chosen. the best amount of antigen was 20 μg/ml at a 1:1000 dilution of the secondary antibody. the cut-off was the average value of all negative serum + 2 deviation standard (0,135). negative serum values were below of cut-off at 410 nm. we do not found differences in detection among strains of t. gondii. these results show that elisa is highly sensitive and specific and is useful to improve efficiency in procedures that involve animals in the laboratory
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