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Search Results: 1 - 10 of 584 matches for " Toxocara eggs "
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Contaminación de suelos con huevos de Toxocara sp. en plazas y parques públicos de la ciudad de La Plata. Buenos Aires, Argentina
Fonrouge,Reinando; Guardis,Mónica del V.; Radman,Nilda E.; Archelli,Susana M.;
Boletín chileno de parasitología , 2000, DOI: 10.4067/S0365-94022000000300009
Abstract: this study consisted of a stratified sampling, randomly taken, of the soil from the squares and parks of the city of la plata, province of buenos aires, in order to establish the prevalence of contamination caused by toxocara sp. a total 242 soil samples was examined. from each sample a 10 grams aliquot was taken, washed in a 0.2% tween 80 solution, and processed using the technique of concentration by flotation with sugar solution. there was a prevalence of 13.2%. in each positive sample, the quantity of eggs varied from 1 to 4. toxocara sp. eggs were observed in 15 out of 22 squares and parks investigated. the sampling design and the processing method employed were satisfactory for the recovering and identification of toxocara sp. eggs
A Method for Accelerating the Maturation of Toxocara cati Eggs
M Zibaei,SM Sadjjadi,SH Jahadi Hosseini,B Sarkari
Iranian Journal of Parasitology , 2007,
Abstract: Background: The effect of temperature and humidity on the maturation of Toxocara cati eggs in an in vitro system was investigated. Methods: Suspensions of Toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °C under 40% humidity or at 25 °C under 98% humidity for 3 weeks for egg development. Results: The suspension sample mixed by 2.5% formalin/ringer and maintained at 25 oC and 98% humidity could fully embryonated the eggs of Toxocara cati in 3 weeks. Conclusion: The main advantage of this method is the increase of recovery and also reducing of the eggs maturation time.
Prevalence of Toxocara species eggs in soil samples of public health importance in and around Bareilly, Uttar Pradesh, India
N. R. Sudhakar,S. Samanta,S. Sahu,O. K. Raina
Veterinary World , 2013, DOI: 10.5455/vetworld.2013.87-90
Abstract: Aim: The objective of this study was to determine the possible extent of soil contamination at different public places with Toxocara species eggs. Materials and Methods: A Total of 327 samples of soil were collected and examined from different locations which are of public health importance like public parks, playgrounds, door mat dusts, Sidewalks or road sides, in Bareilly, Uttar Pradesh, to establish the prevalence of Toxocara eggs. Samples were also categorised in to sandy type (225) and clay type (102) which were examined by Dunsmore modified technique. Results: 42 samples out of 327 (12.84%) were found to be contaminated with the Toxocara spp.eggs and public parks were more contaminated than the other sites we studied. Clay type soil samples were found to be more contaminated than sandy type with a prevalence of 17.64%. Conclusions: The prevalence of this zoonotic parasite in soil has implications for the spread of human disease in these areas. The authors believe that this may constitute a significant health risk, particularly to children. [Vet World 2013; 6(2.000): 87-90]
Prueba de una técnica para recuperar huevos de Toxocara canis de muestras de tierra
SIEVERS,GEROLD; CONCHA,CLAUDIA; GáDICKE,PAULA;
Parasitología latinoamericana , 2007, DOI: 10.4067/S0717-77122007000100010
Abstract: a technique to recovery t. canis eggs from soil samples described in hannover, germany, was adapted to local conditions in valdivia, chile, and it was necessary to test its efficiency. 160 ?trumao type? soil samples of 25 g were prepared adding 354.4 ± 61 eggs oft. canis contained in 0.1 ml of a water suspension. in the adaptation process of the technique, it was prove three variables making 20 repetitions of the following combinations: a) detergent added: acid anionic (ph 4.6), or basic anionic (ph 11.5). b) flotation solution used: sugar solution (density: 1.25), or saturated zinc sulphate (density: 1.38). c) with or without mechanical mixing of the samples previous to the flotation process. the best result, of an average of 50.9% (p < 0.05) of the recovered added t. canis eggs from 25g of ?trumao type? soil was obtained with the addition of acid anionic detergent to the samples, the use of a saturated zinc sulphate solution for the flotation of the eggs and mixing the samples before each flotation process
Comparación de cuatro sistemas de muestreo de tierra para determinar contaminación de áreas con huevos de Toxocara canis
SIEVERS,GEROLD; AMENáBAR,ANDREA; GáDICKE,PAULA;
Parasitología latinoamericana , 2007, DOI: 10.4067/S0717-77122007000100011
Abstract: four soil sampling systems were compared to determine the most representative system to find out the contamination of areas with toxocara canis eggs. in january and february 2006 there were obtained simultaneously 8 accumulative soil samples in courtyards of 12 houses of the city of valdivia with maintained bitches with puppies. the samples were processed in the veterinary parasitology laboratory of the universidad austral de chile. t. canis eggs were found in all courtyards areas. 92 of the analysed soil samples (95.8%) were positive. one of the sampling systems differed (p = 0.13) while the other three were similar (p > 0.05). one of them was choose because the recovery oft. anis eggs was nearly 100% of the mean found in every courtyard
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil
Santarém, Vamilton Alvares;Magoti, Luciana Puga;Sichieri, Tathiana Dias;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000300007
Abstract: the purpose of this study was to evaluate the influence of variables in a flotation technique for the recovery of toxocara canis eggs from soil. the trials were done under standardized conditions on one gram of previously sterilized soil samples contaminated with 200 eggs of t. canis. the following variables were evaluated in serial steps: sieving; type of wash; time of stirring; resuspension of sediment; solution flotation. centrifuge-flotation in sodium nitrate (d = 1.20 g/cm3) was adopted as an initial technique, using tween 80 (0.2%) and decinormal sodium hydroxide as solutions for washing the samples. ten tests were done to compare the variables, using counting in triplicate. the sieving of the material reduced significantly the recovery of eggs (p < 0.001) and the number of eggs recovered was higher when the sediment was resuspended (p < 0.05). after standardization, flotation solutions sodium chloride, zinc sulfate, sodium dichromate, magnesium sulfate, and sodium nitrate (d = 1.20g/cm3) were compared. the best results were obtained by using zinc sulfate solution. in conclusion, the chances of recovering t. canis eggs from samples using flotation solutions can be increased by washing of soil twice using distilled water, and resuspension of sediment. on the other hand, the sieving procedure can drastically reduce the number of eggs.
Comparación de cuatro sistemas de muestreo de tierra para determinar contaminación de áreas con huevos de Toxocara canis COMPARISON OF FOUR SOIL SAMPLING SYSTEMS TO DETERMINE THE CONTAMINATION OF AREAS WITH Toxocara canis EGGS
GEROLD SIEVERS,ANDREA AMENáBAR,PAULA GáDICKE
Parasitología latinoamericana , 2007,
Abstract: Se compararon cuatro sistemas de muestreo de tierra con el propósito de determinar cual es el más representativo de la contaminación de áreas con huevos de Toxocara canis. Entre enero y febrero del 2006, se obtuvieron 8 muestras de tierra en cada uno de 12 patios de casas particulares que tenían perras y cachorros, utilizando simultáneamente los cuatro sistemas de muestreo. Las muestras de tierra fueron procesadas en el Laboratorio de Parasitología Veterinaria de la Universidad Austral de Chile. En todos los patios se encontraron huevos de T. canis. Del total de muestras 92 (95,8%) resultaron positivas a huevos de T. canis. De los cuatro sistemas empleados difirió uno (p = 0,13) que se descartó. Los otros tres sistemas no mostraron diferencias significativas entre si (p > 0,15). Se decide seleccionar uno de ellos como el más representativo por ser el que más se acercó al promedio de 100% de los huevos recuperados en los sitios Four soil sampling systems were compared to determine the most representative system to find out the contamination of areas with Toxocara canis eggs. In January and February 2006 there were obtained simultaneously 8 accumulative soil samples in courtyards of 12 houses of the city of Valdivia with maintained bitches with puppies. The samples were processed in the Veterinary Parasitology Laboratory of the Universidad Austral de Chile. T. canis eggs were found in all courtyards areas. 92 of the analysed soil samples (95.8%) were positive. One of the sampling systems differed (p = 0.13) while the other three were similar (p > 0.05). One of them was choose because the recovery ofT. anis eggs was nearly 100% of the mean found in every courtyard
Prueba de una técnica para recuperar huevos de Toxocara canis de muestras de tierra TEST OF A TECHNIQUE TO RECOVER Toxocara canis EGGS FROM SOIL SAMPLES
GEROLD SIEVERS,CLAUDIA CONCHA,PAULA GáDICKE
Parasitología latinoamericana , 2007,
Abstract: Con el fin de armar una técnica para obtener huevos de Toxocara canis de muestras de tierra en el Laboratorio de Parasitología Veterinaria de la Universidad Austral de Chile, se adaptó, probó y mejoró una técnica descrita en Hannover, Alemania, preparando 160 muestras de 25 g de tierra tipo Trumao a las que se agregó 354,4 + 61 huevos de T. canis contenidos en 0,1 mi de suspensión acuosa. En el proceso de adaptación de la técnica a las condiciones locales, fue necesario probar tres variables realizando 20 repeticiones de las siguientes combinaciones: 1. Tipo de detergente adicionado: aniónico ácido (pH 4,6), o aniónico básico (pH 11,5); 2. Tipo de solución de flotación utilizada: de azúcar (densidad: 1,25), o sulfato de zinc (densidad: 1,38). 3. Con o sin homogenización mecánica de la muestra previa al proceso de flotación. El mejor resultado, de un promedio de 50,9% (p < 0,05) de recuperación de los huevos agregados a muestras de 25 g de tierra de tipo Trumao se obtuvo con la adición de detergente aniónico ácido, la utilización de una solución saturada de sulfato de zinc para la flotación de los huevos y la homogenización de las muestras antes de cada proceso de flotación. Se puede concluir que con las modificaciones realizadas al método de Hannover se aumentó su sensibilidad y es posible recuperar regular y cuantitativamente huevos de T. canis de muestras de suelo de tipo Trumao A technique to recovery T. canis eggs from soil samples described in Hannover, Germany, was adapted to local conditions in Valdivia, Chile, and it was necessary to test its efficiency. 160 Trumao type soil samples of 25 g were prepared adding 354.4 ± 61 eggs ofT. canis contained in 0.1 ml of a water suspension. In the adaptation process of the technique, it was prove three variables making 20 repetitions of the following combinations: a) detergent added: acid anionic (pH 4.6), or basic anionic (pH 11.5). b) Flotation solution used: sugar solution (density: 1.25), or saturated zinc sulphate (density: 1.38). c) with or without mechanical mixing of the samples previous to the flotation process. The best result, of an average of 50.9% (p < 0.05) of the recovered added T. canis eggs from 25g of Trumao type soil was obtained with the addition of acid anionic detergent to the samples, the use of a saturated zinc sulphate solution for the flotation of the eggs and mixing the samples before each flotation process
Comparison of the Biological Properties of Two Strains of Paecilomyces lilacinus (Thom) Samson Associated to Their Antagonistic Effect onto Toxocara canis Eggs
Gortari, M. C.,Galarza, B. C.,Cazau, M. C.,Hours, R. A.
Malaysian Journal of Microbiology , 2008,
Abstract: Biological control of nematodes by fungi is a promising field of application at large scale. Nematode egg is probably the most resistant stage in the nematode life-cycle, however they are susceptible to colonization and destruction by fungal egg-parasites. Fungal extra-cellular enzymes are able to degrade the main chemical constituents of nematode eggshell and at least they are partly responsible for fungal penetration and/or digestion of the internal tissues. Paecilomyces lilacinus was reported as an ovicidal fungus of Toxocara canis eggs in vitro, a parasite of dogs causing toxocarosis. Two wild-type P. lilacinus LPSC # 876 and LPSC # 44 strains, isolated from soil in the main park in the city of La Plata and an agricultural field, respectively, in Argentina, showed antagonism onto T. canis eggs. Besides from the isolation source, both strains showed similar hydrolytic profiles activities, ovicidal effect, and grew well on chitin agar, although no chitinolytic activity was detected. Nevertheless, both strains displayed proteolytic and chitinolytic activities in liquid cultures, with maximum values at 14 days of growth. Variable enzyme activities were observed on carboxymethylcellulose and starch agars, whereas the lipolytic activity was poor in both. Percentage of infected T. canis eggs was found 65.6% and 63.2% for P. lilacinus LPSC # 876 and # 44 strains respectively. Our results a priori indicate that P. lilacinus LPSC # 876 shows suitable characteristics as a potential agent for biocontrol of T. canis.
Frequency of geohelminths in public squares in Pelotas, RS, Brazil Frequência de geohelmintos em pra as públicas de Pelotas, RS, Brasil
Micaele Quintana de Moura,Sabrina Jeske,Juliana Nunes Vieira,Tiago Gallina Corrêa
Revista Brasileira de Parasitologia Veterinária , 2013,
Abstract: The frequency of parasitic contamination of public areas in the municipality of Pelotas, state of Rio Grande do Sul, Brazil, was studied between June 2010 and May 2011, when soil samples were collected from eight city squares. Out of 400 samples submitted to centrifugal floatation technique in solution of sodium dichromate with density of 1.35, 176 (44%) proved positive for at least one parasite; 29 (16.5%) samples were multi-infested. The results showed that there was a significant soil contamination rate in all the parks included in the study. The positivity rate was higher for hookworms eggs (13.5%) and Toxocara eggs (8.8%); Trichuris, Ascaris and Capillaria eggs were also detected. This study shows the risks to which the population is exposed in relation to zoonotic geohelminths, and suggests that sanitation and health education measures should be implemented in the municipality. A frequência de contamina o parasitária de áreas públicas de Pelotas, Rio Grande do Sul, Brasil, foi avaliada entre junho de 2010 e maio de 2011, com coletas mensais de amostras de solo de oito pra as. Das 400 amostras submetidas à técnica de centrífugo-flutua o em solu o de dicromato de sódio com densidade de 1,35, 176 (44%) apresentaram pelo menos uma forma parasitária e, das amostras positivas, 29 (16,5%) estavam poliparasitadas. Os resultados demonstraram relevante índice de contamina o do solo em todas as pra as avaliadas, com maiores índices de positividade para ovos de ancilostomídeos (13,5%) e ovos de Toxocara (8,8%), sendo também identificados ovos de Trichuris, Ascaris e Capillaria. O estudo demonstrou a contamina o ambiental de pra as públicas e os riscos a que a popula o está exposta em rela o a doen as causadas por geoparasitos zoonóticos e sugere que medidas de saneamento e educa o em saúde devem ser implementadas no município.
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