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Search Results: 1 - 10 of 1101 matches for " Tomoko Matsubara "
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Development of Preparative Chromatography for Proteomic Approach of Mycorrhizal Symbiosis  [PDF]
Tomoko Matsubara, Takaaki Ishii
Advances in Biological Chemistry (ABC) , 2015, DOI: 10.4236/abc.2015.51002
Abstract: Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.
Fungal Isolate “KMI” Is a New Type of Orchid Mycorrhizal Fungus  [PDF]
Tomoko Matsubara, Motohito Yoneda, Takaaki Ishii
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.38135
Abstract: We analyzed an isolate of fungus that seems to be a new type orchid mycorrhizal fungus, “KMI (refers to Kyoto-Ma- tsubara-Ishii)”, obtained from the roots of Paphiopedilum thailandense Fowl. KMI has hyphal branching into right angle similar to Rhizoctonia-like fungi, but its spore formation resembled Nectoria, which is known as a teleomorph of Fusarium rather than Rhizoctonia. Its ribosomal DNA sequences of 18S and ITS have no similarity with any known fungal species. Proteins in molecular weight of 53 and 24 kDa, which are common to mycorrhizal fungi, were detected in KMI. When KMI was inoculated onto orchids, however it didn’t form pelotons or coils, but the hyphae in the root tissues were observed and the tissues were not decayed. No significant symptoms of Rhizoctonia or Fusarium disease, however, were developed on tomato and cucumber plants. The evidences suggest that KMI is a new type of orchid mycorrhizal fungus.
Prognostic Value of SUVmax of 18F-FDG PET/CT in Early Stage Breast Cancer with No LN Metastasis  [PDF]
Ryusuke Murakami, Yoshimitsu Fukushima, Hitomi Tani, Kotomi Iwata, Shinichiro Kumita, Maki Nakai, Tomoko Kurita, Keiko Yanagihara, Hiroyuki Takei, Miyuki Matsubara
Open Journal of Medical Imaging (OJMI) , 2017, DOI: 10.4236/ojmi.2017.73011
Abstract: Purpose: To investigate the correlation between SUVmax of FDG-PET/CT and pathological findings including prognostic factors in early-stage T1-T2 breast cancer patients with no LN metastasis. Materials and Methods: This retrospective study investigated 75 patients (mean age 58.9 years; age range 30 - 82 years) with invasive breast cancer who underwent FDG-PET/CT for preoperative staging. All patients underwent subsequent surgery without prior neoadjuvant chemotherapy or endocrine therapy, and those who were confirmed to have T1- or T2-stage by histopathology with no LN metastasis were included. Two patients who had no perceptible FDG accumulation on PET/CT scans were excluded. The correlations between the SUVmax of the tumor and the pathological and immunohistochemical data were evaluated. Results: The mean SUVmax for the total 73 tumors was 5.46 ± 4.05. The mean SUVmax was 3.95 ± 3.28 for the T1 stage group (n = 36) and 7.23 ± 4.10 (p < 0.001) for the T2 stage group (n = 37). A high SUVmax was significantly associated with high nuclear grade (p < 0.001), negative hormone receptor status (p < 0.001), positive HER2 status (p = 0.008), and high Ki-67 status (p < 0.001), respectively. Conclusion: In T1-T2 breast cancer with no LN metastasis, the SUVmax of FDG-PET/CT had significant positive relationships with several prognostic parameters of pathological status. Even in early-stage breast cancer patients, pretreatment FDG-PET/CT is useful for predicting malignant behavior and prognosis).
Results of Dating Violence Prevention Education for Japanese High School Boys  [PDF]
Tomoko Suga
Open Journal of Social Sciences (JSS) , 2017, DOI: 10.4236/jss.2017.512013
We conducted a class on domestic violence (DV) prevention for 234 high school boys (intervention group: 154 boys, non-intervention group: 80 boys) and verified its effect on the boys. The program dealt with respect, relationships and types of violence. The factor analysis of a questionnaire survey conducted before the class on DV prevention revealed that the high school boys’ understanding of “a relationship” and “a coercive behavior” was weak. Therefore, after conducting the class on DV prevention, we tested whether there was an improvement in their understanding of the terms “relationship” and “coercive behavior”. To understand whether boys’ understanding of “relationships” showed any change after the class, a comparison of the intervention and the non-intervention groups was carried out at four different time points—before the class on DV prevention, after the class, after a month, and after six months. A 2-factorial analysis of variance (repeated measures) was conducted. The results revealed that no mutual points of interaction were seen with the different measurement times based on the presence or absence of intervention (F (3,696) = 0.995, n.s.). To understand whether the term “coercive behaviors” changed boys’ understanding of the term after the class, the comparison from the results before the class till six months later showed significant mutual interactions with the measurement times based on the presence or absence of intervention (F (3,696) = 4.48, p < 0.01). From this study, it is clear that interventions such as a single class on DV prevention can help boys understand the term “coercive acts” and have an impact on their minds for a long time. However, the same may not be true in their understanding of “relationships”.
Japanese Educators’ Knowledge of DV  [PDF]
Tomoko Suga
Open Journal of Social Sciences (JSS) , 2018, DOI: 10.4236/jss.2018.62002
In the present research, a survey of the Domestic Violence (DV) related experience, knowledge, understanding of characteristics, and opinions on prevention of 244 Japanese educators was conducted. It cannot be overlooked that 8.0% of the 244 educators had experience suffering from DV. Additionally, 28.8% of the educators had experience taking a training or class on DV, and 34.0% had experience studying DV through books, etc. It was revealed from the present survey that roughly 30% of the educators had educational experience related to DV. Also it was revealed that 90.7% of female educators and 85.9% of male educators who were subjects of the present study thought that “It’s best if DV prevention is implemented during middle school and high school classes.” Educators who had studied DV through books, etc. understood six out of seven items about the characteristics of DV, which was more than those who had not studied DV. Additionally, those who had experience taking a training or class understood in detail three questions out of seven, which was more than those without experience. It is important to provide opportunities for training that would lead to learn the correct knowledge about DV for educators.
An Analysis of Surveys on Domestic Violence by Japan’s Cabinet Office (1999-2017)  [PDF]
Tomoko Suga
Open Journal of Social Sciences (JSS) , 2018, DOI: 10.4236/jss.2018.67005
Abstract: Japan’s Cabinet Office conducted surveys on DV seven times (1999, 2002, 2005, 2008, 2011, 2014, 2017). As a secondary source, we used the data on the Cabinet Offices website. In 2001, the first DV law, the Act on the Prevention of Spousal Violence was passed, after which the Japanese public began to recognize DV. Based on the question item on “domestic violence from a spouse” the data of the Cabinet Office’s survey from 2005 to 2017 indicated that on average, just over 30% of women experienced DV, while just under 20% of men did. These figures have not changed much. The proportions of victims who use advisory services have increased with every survey due to political movements and Japanese policy. In 2017, the data showed that a little less than 58% of women and almost 27% of men used advisory services. The most recent survey conducted in 2017, showed that people who do not separate from their spouse experience DV. In terms of the reasons that they do not separate, “children” weighed high on the list.
The Construction of Pairwise Additive Minimal BIB Designs with Asymptotic Results  [PDF]
Kazuki Matsubara, Sanpei Kageyama
Applied Mathematics (AM) , 2014, DOI: 10.4236/am.2014.514207
Abstract: An asymptotic existence of balanced incomplete block (BIB) designs and pairwise balanced designs (PBD) has been discussed in [1]-[3]. On the other hand, the existence of additive BIB designs and pairwise additive BIB designs with k = 2 and λ = 1 has been discussed with direct and recursive constructions in [4]-[8]. In this paper, an asymptotic existence of pairwise additive BIB designs is proved by use of Wilson’s theorem on PBD, and also for some and k the exact existence of l pairwise additive BIB designs with block size k and λ = 1 is discussed.
Economic and Social Impact of Tourism on a Small Town: Peterborough New Hampshire  [PDF]
Tomoko Tsundoda, Samuel Mendlinger
Journal of Service Science and Management (JSSM) , 2009, DOI: 10.4236/jssm.2009.22009
Abstract: This study examined the perceived impacts of tourism on the lives of people from a small New England town. Twenty seven in-depth unstructured interviews were conducted. The study found that most people perceive both positive and negative impacts of tourism and do not want to change their town for increased tourism development even if it results in increased revenue. People recognized tourism’s benefit to the town’s economy but less so to their economic situation. Working locals expressed worry regarding the town’s gradually polarized economy and divided social classes and regard tourism as one of the causes. Wealthier members generally view tourism at its present level as beneficial. For further tourism development Peterborough’ population will need to solve this dilemma.
A Multi-Secret Sharing Scheme with Many Keys Based on Hermite Interpolation  [PDF]
Tomoko Adachi, Chie Okazaki
Journal of Applied Mathematics and Physics (JAMP) , 2014, DOI: 10.4236/jamp.2014.213140
Abstract: A secret sharing scheme is one of cryptographies. A threshold scheme, which is introduced by Shamir in 1979, is very famous as a secret sharing scheme. We can consider that this scheme is based on Lagrange's interpolation formula. A secret sharing scheme has one key. On the other hand, a multi-secret sharing scheme has more than one keys; that is, a multi-secret sharing scheme has p (2) keys. Dealers distribute shares of keys among n participants. Gathering t (n) participants, keys can be reconstructed. In this paper, we give a scheme of a (t,n) multi-secret sharing based on Hermite interpolation, in the case of pt.
Some Sequence of Wrapped Δ-Labellings for the Complete Bipartite Graph  [PDF]
Tomoko Adachi, Daigo Kikuchi
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.61019
Abstract: The design of large disk array architectures leads to interesting combinatorial problems. Minimizing the number of disk operations when writing to consecutive disks leads to the concept of “cluttered orderings” which were introduced for the complete graph by Cohen et al. (2001). Mueller et al. (2005) adapted the concept of wrapped Δ-labellings to the complete bipartite case. In this paper, we give some sequence in order to generate wrapped Δ-labellings as cluttered orderings for the complete bipartite graph. New sequence we give is different from the sequences Mueller et al. gave, though the same graphs in which these sequences are labeled.
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