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Rockfalls Stara gora near Dvor in municipality u emberk
Magda ?arman,Tina Peternel
Geologija , 2010,
Abstract: In Slovenia, an increased number of rockfall events have been noticed in the last years. They were most likely inrelation to weather extremes. At Stara gora near Dvor in municipality u emberk, fallen blocks of carbonate rockhave already caused damage on a residential object and represent a danger to real property and human lives. Theblocks source area is situated in the u emberk fault zone. The carbonate rocks in the fault zone are tectonicallyhighly disturbed, which leads to their rapid erosion and disintegration. The rockfall events are a result of the ridgeerosion and will also appear in the future. Hazard degree assessment and protection assessment were based on theresults of detailed geological and engineering geological mapping and rock fall simulation with RocFall computersoftware. A rock fall hazard map was also produced.
Inclusion bodies as potential vehicles for recombinant protein delivery into epithelial cells
Mirjana Liovic, Mateja Ozir, Apolonija Bedina Zavec, Spela Peternel, Radovan Komel, Tina Zupancic
Microbial Cell Factories , 2012, DOI: 10.1186/1475-2859-11-67
Abstract: K14 IBs were electroporated into SW13 cells grown in culture together with a “reporter” plasmid containing EYFP labeled keratin 5 (K5) cDNA. As SW13 cells do not normally express keratins, and keratin filaments are built exclusively of keratin heterodimers (i.e. K5/K14), the short filamentous structures we obtained in this study can only be the result of: a) if both IBs and plasmid DNA are transfected simultaneously into the cell(s); b) once inside the cells, K14 protein is being released from IBs; c) released K14 is functional, able to form heterodimers with EYFP-K5.Soluble IBs may be also developed for complex cytoskeletal proteins and used as nanoparticles for their delivery into epithelial cells.
Active Protein Aggregates Produced in Escherichia coli
?pela Peternel,Radovan Komel
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12118275
Abstract: Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is?increasing.
Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells
?pela Peternel, Radovan Komel
Microbial Cell Factories , 2010, DOI: 10.1186/1475-2859-9-66
Abstract: To prepare large quantities of a high quality product, the whole bioprocess has to be optimised. This includes not only the cultivation of the bacterial culture, but also the isolation step itself, which can be of critical importance for the production process.To determine the most appropriate method for the isolation of biologically active nanoparticles, three methods for bacterial cell disruption were analyzed.In this study, enzymatic lysis and two mechanical methods, high-pressure homogenization and sonication, were compared.During enzymatic lysis the enzyme lysozyme was found to attach to the surface of IBs, and it could not be removed by simple washing. As this represents an additional impurity in the engineered nanoparticles, we concluded that enzymatic lysis is not the most suitable method for IBs isolation.During sonication proteins are released (lost) from the surface of IBs and thus the surface of IBs appears more porous when compared to the other two methods. We also found that the acoustic output power needed to isolate the IBs from bacterial cells actually damages proteins structures, thereby causing a reduction in biological activity.High-pressure homogenization also caused some damage to IBs, however the protein loss from the IBs was negligible. Furthermore, homogenization had no side-effects on protein biological activity.The study shows that among the three methods tested, homogenization is the most appropriate method for the isolation of active nanoparticles from bacterial cells.In recent years, the rapid expansion of biotechnology has lead to the production of a wide spectrum of recombinant proteins. To this end, a range of host organisms, from bacteria to mammalian cell-culture systems are being used. Even though bacteria have some disadvantages, Escherichia coli is still one of the most commonly used organisms for the production of recombinant proteins [1-3].The over-expression of recombinant proteins in bacteria often leads to their aggregation
Cosmetic Surgery as Intrasexual Competition: The Mediating Role of Social Comparison  [PDF]
Steven Arnocky, Tina Piché
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.510132

Cosmetic surgical procedures have previously been associated with some risks to psychological and physical health. Yet such procedures are on the rise, highlighting the need for a better understanding of the factors which might underlie the decision to undergo cosmetic surgery. In a sample of 297 young adults (192 women), we examined the relationship between intrasexual competition (IC), social comparison, and individuals’ attitudes, perceived risks, and desired spending on cosmetic surgical procedures. Results showed that women perceived more risk to cosmetic surgery, yet held more positive attitudes and desire to spend on cosmetic surgery compared to men. For both men and women, IC predicted positive attitudes and desired spending on cosmetic surgery. Social comparison mediated all relationships between IC and cosmetic surgery variables. Cosmetic surgery is discussed as a potential form of intrasexual competition rooted in the mate-preferences of the opposite sex.

Comparative Analysis of Cartographic Literacy in the Selected Curricula at the Primary Level  [PDF]
Vlasta Hus, Tina Hojnik
Creative Education (CE) , 2013, DOI: 10.4236/ce.2013.412107
Abstract: Basic knowledge of maps, because of its widespread use, has become a part of the individual’s modern literacy. This is why we were interested in how this kind of literacy is represented in schools, when it occurs and how it is upgraded. Based on the study of literature we have formed criteria of cartographic literacy and based on the comparative method we have compared curricula for the lower stage of primary education of two countries (England and Slovenia). We found that cartographic literacy is given most attention, as well as in content as in didactic recommendations, in the Slovene curriculum for Social Studies (second triad). But according to the pupils’ cognitive abilities, it is being introduced too late. In the English curriculum for Geography initial cartography is far more dependent on the teachers and their cartography knowledge (or lack of it) because of the general definition of objectives and lack of recommendations. A combination of such an open curriculum and teacher’s cartography knowledge (or lack of it) can push cartographic literacy a step back from other content. That is why educating teachers and paying attention to the matter either in the curriculum or in textbook sets is of utmost importance.
Cognitive Behavior Therapy of Urinary Incontinence Phobia: A Case Report  [PDF]
Tina Tan, Leslie Lim
Open Journal of Psychiatry (OJPsych) , 2015, DOI: 10.4236/ojpsych.2015.51009
Abstract: A significant association exists between lower urinary tract symptoms and anxiety. Despite the efficacy of cognitive behavior therapies in treating a variety of phobic disorders, there are few published reports about the use of such treatments for urinary problems. We report a case of the phobia of urinary incontinence treated with cognitive behavioral techniques, which involved cognitive restructuring, distraction, relaxation training, and exposure to feared situations.
A Presence-Based Context-Aware Chronic Stress Recognition System
Klemen Peternel,Matev? Poga?nik,Rudi Tav?ar,Andrej Kos
Sensors , 2012, DOI: 10.3390/s121115888
Abstract: Stressors encountered in daily life may play an important role in personal well-being. Chronic stress can have a serious long-term impact on our physical as well as our psychological health, due to ongoing increased levels of the chemicals released in the ‘fight or flight’ response. The currently available stress assessment methods are usually not suitable for daily chronic stress measurement. The paper presents a context-aware chronic stress recognition system that addresses this problem. The proposed system obtains contextual data from various mobile sensors and other external sources in order to calculate the impact of ongoing stress. By identifying and visualizing ongoing stress situations of an individual user, he/she is able to modify his/her behavior in order to successfully avoid them. Clinical evaluation of the proposed methodology has been made in parallel by using electrodermal activity sensor. To the best of our knowledge, the system presented herein is the first one that enables recognition of chronic stress situations on the basis of user context.
Engineering inclusion bodies for non denaturing extraction of functional proteins
?pela Peternel, Joze Grdadolnik, Vladka Gaberc-Porekar, Radovan Komel
Microbial Cell Factories , 2008, DOI: 10.1186/1475-2859-7-34
Abstract: Four structurally different proteins that accumulate in the bacterial cell in the form of IBs were studied, revealing that distribution of each target protein between the soluble fraction (cytoplasm) and insoluble fraction (IBs) depends on the nature of the target protein.Irrespective of the folding pattern of each protein, spectroscopy studies have shown that proteins in IBs exhibit similar structural characteristics to the biologically active pure protein when produced at low temperature. In the case of the three studied proteins, G-CSF, His7ΔN6TNF-α, and GFP, a significant amount of protein could be extracted from IBs with 0.2% N-lauroyl sarcosine (NLS) and the proteins retained biological activity although no renaturation procedure was applied.This study shows that the presence of biologically active proteins inside IBs is more general than usually believed. A large amount of properly folded protein is trapped inside IBs prepared at lower temperatures. This protein can be released from IBs with mild detergents under non-denaturing conditions. Therefore, the active protein can be obtained from such IBs without any renaturation procedure. This is of great importance for the biopharmaceutical industry. Furthermore, such IBs composed of active proteins could also be used as pure nanoparticles in diagnostics, as biocatalysts in enzymatic processes, or even as biopharmaceuticals.The formation of inclusion bodies (IBs) upon overexpression of heterologous proteins in Escherichia coli (E. coli) is a common event. Because of the belief that IBs are intracellular deposits of misfolded, biologically inactive proteins, they have been considered to be a bottleneck in protein production. To avoid time consuming and often unfavorable renaturation procedures, a lot of effort has been made to produce soluble proteins in bacteria either by altering the production process [1,2], by co-expression of chaperones [3,4] or by altering the target protein (point mutations, fusion proteins
Role of quantum dots nanoparticles in the chemical treatment of colored wastewater: Catalysts or additional pollutants

Hrvoje Kusi,Danuta Leszczynsk,Natalija Koprivanac,Igor Peternel,

环境科学学报(英文版) , 2011,
Abstract: The objective of the study was to investigate the presence and the activity of quantum dots nanoparticles in colored wastewaters. The special interest is devoted to the investigation of their role in the typical treatment of water or wastewater, studying their influence on the effectiveness of applied treatments methods. The standard chemical processes for water treatment and disinfection (direct UV photolysis and direct ozonation) were applied for the degradation of colored organic pollutant, reactive azo dye, in the presence/absence of CdSe/ZnS core-shells quantum dots. The obtained results indicated that investigated nanoparticles inhibit the overall efficiency of applied processes, especially in the case of direct UV photolysis, although catalytic effect might be expected in part due to the semiconductor nature of quantum dots. Such results lead to a conclusion that CdSe/ZnS nanoparticles behave as additional pollutants in the system. They should be removed from the system prior the treatment, because their presence could decrease the efficiency, i.e., prolong the time of treatment and correspondingly increase the costs of the treatment process.
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