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Search Results: 1 - 10 of 3210 matches for " Tien-Chueh Kuo "
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Metabolomic Dynamic Analysis of Hypoxia in MDA-MB-231 and the Comparison with Inferred Metabolites from Transcriptomics Data
I-Lin Tsai,Tien-Chueh Kuo,Tsung-Jung Ho,Yeu-Chern Harn,San-Yuan Wang,Wen-Mei Fu,Ching-Hua Kuo,Yufeng Jane Tseng
Cancers , 2013, DOI: 10.3390/cancers5020491
Abstract: Hypoxia affects the tumor microenvironment and is considered important to metastasis progression and therapy resistance. Thus far, the majority of global analyses of tumor hypoxia responses have been limited to just a single omics level. Combining multiple omics data can broaden our understanding of tumor hypoxia. Here, we investigate the temporal change of the metabolite composition with gene expression data from literature to provide a more comprehensive insight into the system level in response to hypoxia. Nuclear magnetic resonance spectroscopy was used to perform metabolomic profiling on the MDA-MB-231 breast cancer cell line under hypoxic conditions. Multivariate statistical analysis revealed that the metabolic difference between hypoxia and normoxia was similar over 24 h, but became distinct over 48 h. Time dependent microarray data from the same cell line in the literature displayed different gene expressions under hypoxic and normoxic conditions mostly at 12 h or earlier. The direct metabolomic profiles show a large overlap with theoretical metabolic profiles deduced from previous transcriptomic studies. Consistent pathways are glycolysis/gluconeogenesis, pyruvate, purine and arginine and proline metabolism. Ten metabolic pathways revealed by metabolomics were not covered by the downstream of the known transcriptomic profiles, suggesting new metabolic phenotypes. These results confirm previous transcriptomics understanding and expand the knowledge from existing models on correlation and co-regulation between transcriptomic and metabolomics profiles, which demonstrates the power of integrated omics analysis.
Accelerated Neuronal Cell Recovery from Botulinum Neurotoxin Intoxication by Targeted Ubiquitination
Chueh-Ling Kuo,George A. Oyler,Charles B. Shoemaker
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0020352
Abstract: Botulinum neurotoxin (BoNT), a Category A biodefense agent, delivers a protease to motor neuron cytosol that cleaves one or more soluble NSF attachment protein receptors (SNARE) proteins involved in neurotransmission to cause a flaccid paralysis. No antidotes exist to reverse symptoms of BoNT intoxication so severely affected patients require artificial respiration with prolonged intensive care. Time to recovery depends on toxin serotype because the intraneuronal persistence of the seven known BoNT serotypes varies widely from days to many months. Our therapeutic antidote strategy is to develop ‘targeted F-box’ (TFB) agents that target the different intraneuronal BoNT proteases for accelerated degradation by the ubiquitin proteasome system (UPS), thus promoting rapid recovery from all serotypes. These agents consist of a camelid heavy chain-only VH (VHH) domain specific for a BoNT protease fused to an F-box domain recognized by an intraneuronal E3-ligase. A fusion protein containing the 14 kDa anti-BoNT/A protease VHH, ALcB8, joined to a 15 kDa F-box domain region of TrCP (D5) was sufficient to cause increased ubiquitination and accelerate turnover of the targeted BoNT/A protease within neurons. Neuronal cells expressing this TFB, called D5-B8, were also substantially resistant to BoNT/A intoxication and recovered from intoxication at least 2.5 fold quicker than control neurons. Fusion of D5 to a VHH specific for BoNT/B protease (BLcB10) led to accelerated turnover of the targeted protease within neurons, thus demonstrating the modular nature of these therapeutic agents and suggesting that development of similar therapeutic agents specific to all botulinum serotypes should be readily achievable.
Medical Organization Information Security Management Based on ISO27001 Information Security Standard
Kuo-Hsiung Liao,Hao-En Chueh
Journal of Software , 2012, DOI: 10.4304/jsw.7.4.792-797
Abstract: Most of the information security events in medical organizations are due to improper management. This is a clear indication that the security of information is an issue related to information and communication technology and a management issue as well. In a review of literature, most research on information security has focused on information and communication technology issues, such as network security and access control; rarely addressing issues at the management-level. The main purpose of this study is to construct a mechanism for the management of information with regard to security as it applies to medical organizations. This mechanism is based on the eleven control items and one hundred thirty-three control objectives of the ISO27001 information security management standard. This study analyzes and identifies the most common events related to information security in medical organizations and categorizes these events as high risk, transferable-risk, and controlled-risk to facilitate the management of such risk.
Role of Glutathione in the Regulation of Cisplatin Resistance in Cancer Chemotherapy
Helen H. W. Chen,Macus Tien Kuo
Metal-Based Drugs , 2010, DOI: 10.1155/2010/430939
Efficient Formulations for 1-SVM and their Application to Recommendation Tasks
Yasutoshi Yajima,Tien-Fang Kuo
Journal of Computers , 2006, DOI: 10.4304/jcp.1.3.27-34
Abstract: The present paper proposes new approaches for recommendation tasks based on one-class support vector machines (1-SVMs) with graph kernels generated from a Laplacian matrix. We introduce new formulations for the 1-SVM that can manipulate graph kernels quite efficiently. We demonstrate that the proposed formulations fully utilize the sparse structure of the Laplacian matrix, which enables the proposed approaches to be applied to recommendation tasks having a large number of customers and products in practical computational times. Results of various numericalexperiments demonstrating the high performance of the proposed approaches are presented.
The Relationship of Arginine Deprivation, Argininosuccinate Synthetase and Cell Death in Melanoma
Niramol Savaraj,Chunjing Wu,Marcus Tien Kuo,Min You
Drug Target Insights , 2007,
Abstract: It has been shown that melanoma cells do not express argininosuccinate synthetase (ASS) and therefore are unable to synthesize arginine from citrulline. Depleting arginine using pegylated arginine deiminase (ADI-PEG20) results in cell death in melanoma but not normal cells. This concept was translated into clinical trial and responses were seen. However, induction of ASS expression does occur which results in resistance to ADI -PEG20. We have used 4 melanoma cell lines to study factors which may govern ASS expression. Although these 4 melanoma cell lines do not express ASS protein or mRNA as detected by both immunoblot and northernblot analysis, ASS protein can be induced after these cells are grown in the presence of ADI-PEG20, but again repressed after replenishing arginine in the media. The levels of induction are different and one cell line could not be induced. Interestingly, a melanoma cell line with the highest level of induction could also be made resistant to ADI-PEG20. This resistant line possesses high levels of ASS mRNA and protein expression which cannot be repressed with arginine. Our study indicates that ASS expression in melanoma cells is complex and governed by biochemical parameters which are different among melanoma cells.
Fabrication of Al-Doped TiO2 Visible-Light Photocatalyst for Low-Concentration Mercury Removal
Cheng-Yen Tsai,Tien-Ho Kuo,Hsing-Cheng Hsi
International Journal of Photoenergy , 2012, DOI: 10.1155/2012/874509
Abstract: High-quality Al-doped TiO2 visible-light photocatalyst was prepared via a single-step direct combination of vaporized Ti, Al, and O2 using a 6 kW thermal plasma system. Results showed that the formed Al-doped TiO2 nanoparticles were a mixture of anatase and rutile phase and had a size between 10 and 105 nm. The absorption spectra of the nanoparticles shifted towards the visible light regions, depending on the Al2O3 addition. Ti4
Enterovirus 71 3C Protease Cleaves a Novel Target CstF-64 and Inhibits Cellular Polyadenylation
Kuo-Feng Weng,Mei-Ling Li,Chuan-Tien Hung,Shin-Ru Shih
PLOS Pathogens , 2009, DOI: 10.1371/journal.ppat.1000593
Abstract: Identification of novel cellular proteins as substrates to viral proteases would provide a new insight into the mechanism of cell–virus interplay. Eight nuclear proteins as potential targets for enterovirus 71 (EV71) 3C protease (3Cpro) cleavages were identified by 2D electrophoresis and MALDI-TOF analysis. Of these proteins, CstF-64, which is a critical factor for 3′ pre-mRNA processing in a cell nucleus, was selected for further study. A time-course study to monitor the expression levels of CstF-64 in EV71-infected cells also revealed that the reduction of CstF-64 during virus infection was correlated with the production of viral 3Cpro. CstF-64 was cleaved in vitro by 3Cpro but neither by mutant 3Cpro (in which the catalytic site was inactivated) nor by another EV71 protease 2Apro. Serial mutagenesis was performed in CstF-64, revealing that the 3Cpro cleavage sites are located at position 251 in the N-terminal P/G-rich domain and at multiple positions close to the C-terminus of CstF-64 (around position 500). An accumulation of unprocessed pre-mRNA and the depression of mature mRNA were observed in EV71-infected cells. An in vitro assay revealed the inhibition of the 3′-end pre-mRNA processing and polyadenylation in 3Cpro-treated nuclear extract, and this impairment was rescued by adding purified recombinant CstF-64 protein. In summing up the above results, we suggest that 3Cpro cleavage inactivates CstF-64 and impairs the host cell polyadenylation in vitro, as well as in virus-infected cells. This finding is, to our knowledge, the first to demonstrate that a picornavirus protein affects the polyadenylation of host mRNA.
A Novel pH-dependent Drift Improvement Method for Zirconium Dioxide Gated pH-Ion Sensitive Field Effect Transistors
Kow-Ming Chang,Chih-Tien Chang,Kuo-Yi Chao,Chia-Hung Lin
Sensors , 2010, DOI: 10.3390/s100504643
Abstract: A novel compensation method for Zirconium dioxide gated Ion Sensitive Field Effect Transistors (ISFETs) to improve pH-dependent drift was demonstrated. Through the sequential measurements for both the n-channel and p-channel ISFETs, 75–100% pH-dependent drift could be successfully suppressed for the first seven hours. As a result, a nearly constant drift rate versus pH value was obtained, which increases the accuracy of pH measurements. Meanwhile, the drawback of the hyperbolic-like change with time of the common drift behavior for ISFETs was improved. A state-of-the-art integrated scheme adopting this method was also illustrated.
The absorption and uptake of recombinant human follicle-stimulating hormone through vaginal subcutaneous injections - a pharmacokinetic study
Chao-Chin Hsu, Hsin-Chih Kuo, Chao-Tien Hsu, Qing Gu
Reproductive Biology and Endocrinology , 2009, DOI: 10.1186/1477-7827-7-107
Abstract: Twelve healthy women with regular ovulatory cycles were recruited. All volunteers received gonadotrophin-releasing hormone agonist to suppress pituitary function and were assigned to receive single dose recombinant human FSH (rhFSH, Puregon 300) either using conventional abdominal subcutaneous injection or vaginal subcutaneous injection in a randomized cross-over study. Serum samples were collected at pre- scheduled time intervals after injections of rhFSH to determine immunoreactive FSH levels. Pharmacokinetic parameters characterizing rate [maximal plasma concentrations (Cmax) and time of maximal plasma concentrations (tmax)] and extent [area under the plasma concentration-time curve (AUC) and clearance] of absorption of rhFSH were compared.Vaginal injection of rhFSH was well tolerated and no drug-related adverse reaction was noted. Our analysis revealed that tmax was significantly earlier (mean 6.67 versus 13.33 hours) and Cmax was significantly higher (mean 17.77 versus 13.96 IU/L) in vaginal versus abdominal injections. The AUC0-∞ was 1640 versus 1134 IU·hour/L in vaginal and abdominal injections, respectively. Smaller plasma elimination rate constant (0.011 versus 0.016 hour-1), longer mean residence time (106.58 versus 70.47 hours), and slower total body clearance (292.2 versus 400.1 mL/hour) were also found in vaginal injection.The vaginal injection mode elicited a rapid and highly extended absorption of rhFSH injected compared to conventional abdominal injection. These data indicate that the rate and extent of FSH absorption from the injection site can vary depending on the route of the FSH administration.Follicle stimulating hormone (FSH) has been widely employed for ovulation induction and in-vitro fertilization. The ability of FSH to promote folliculogenesis is dependent on the plasma level of FSH that develops after administration [1-3]. The relatively short elimination half-life and rapid metabolic clearance of current FSH preparations requires that da
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