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Search Results: 1 - 10 of 215639 matches for " Terri L. Young "
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Quality of DNA Extracted from Mouthwashes
Tetyana Zayats, Terri L. Young, David A. Mackey, Fran?ois Malecaze, Patrick Calvas, Jeremy A. Guggenheim
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0006165
Abstract: Background A cost effective, safe and efficient method of obtaining DNA samples is essential in large scale genetic analyses. Buccal cells are an attractive source of DNA, as their collection is non-invasive and can be carried out by mail. However, little attention has been given to the quality of DNA extracted from mouthwashes. Methodology Mouthwash-derived DNA was extracted from 500 subjects participating in a genetic study of high myopia. DNA quality was investigated using two standard techniques: agarose gel electrophoresis and quantitative polymerase chain reaction (qPCR). Principal Findings Whereas the majority of mouthwash-derived DNA samples showed a single band of high molecular weight DNA by gel electrophoresis, 8.9% (95% CI: 7.1–10.7%) of samples contained only a smear of low-to-medium molecular weight, degraded DNA. The odds of DNA degradation in a subject's second mouthwash sample, given degradation of the first, was significantly greater than one (OR = 3.13; 95% CI: 1.22–7.39; Fisher's test P = 0.009), suggesting that DNA degradation was at least partially a subject-specific phenomenon. Approximately 12.4% (95% CI: 10.4–14.4%) of mouthwash-derived DNA failed to PCR amplify efficiently (using an ~200 bp microsatellite marker). However, we found there was no significant difference in amplification success rate between DNA samples judged to be degraded or non-degraded by gel electrophoresis (Fisher's test P = 0.5). Conclusions This study demonstrated that DNA degradation affects a significant minority of saline mouthwashes, and that the phenomenon is partially subject-specific. Whilst the level of degradation did not significantly prevent successful amplification of short PCR fragments, previous studies suggest that such DNA degradation would compromise more demanding applications.
Scleral Micro-RNA Signatures in Adult and Fetal Eyes
Ravikanth Metlapally, Pedro Gonzalez, Felicia A. Hawthorne, Khanh-Nhat Tran-Viet, Christine F. Wildsoet, Terri L. Young
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078984
Abstract: Introduction In human eyes, ocular enlargement/growth reflects active extracellular matrix remodeling of the outer scleral shell. Micro-RNAs are small non-coding RNAs that regulate gene expression by base pairing with target sequences. They serve as nodes of signaling networks. We hypothesized that the sclera, like most tissues, expresses micro-RNAs, some of which modulate genes regulating ocular growth. In this study, the scleral micro-RNA expression profile of rapidly growing human fetal eyes was compared with that of stable adult donor eyes using high-throughput microarray and quantitative PCR analyses. Methods Scleral samples from normal human fetal (24 wk) and normal adult donor eyes were obtained (n=4 to 6, each group), and RNA extracted. Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform. Micro-RNA target predictions were obtained using Microcosm, TargetScan and PicTar algorithms. TaqMan? micro-RNA assays targeting micro-RNAs showing either highest significance, detection, or fold differences, and collagen specificity, were applied to scleral samples from posterior and peripheral ocular regions (n=7, each group). Microarray data were analyzed using R, and quantitative PCR data with 2^-deltaCt methods. Results Human sclera was found to express micro-RNAs, and comparison of microarray results for adult and fetal samples revealed many to be differentially expressed (p<0.01, min p= 6.5x1011). Specifically, fetal sclera showed increased expression of mir-214, let-7c, let-7e, mir-103, mir-107, and mir-98 (1.5 to 4 fold changes, p<0.01). However, no significant regionally specific differences .i.e., posterior vs. peripheral sclera, were observed for either adult or fetal samples. Conclusion For the first time, micro-RNA expression has been catalogued in human sclera. Some micro-RNAs show age-related differential regulation, higher in the sclera of rapidly growing fetal eyes, consistent with a role in ocular growth regulation. Thus micro-RNAs represent potential targets for ocular growth manipulation, related to myopia and/or other disorders such as scleral ectasia.
The Effects of Fetal Surgery on Retinopathy of Prematurity Development
Sudha Nallasamy, Stefanie L. Davidson, Lori J. Howell, Holly Hedrick, Alan W. Flake, Timothy M. Crombleholme, N. Scott Adzick and Terri L. Young
Ophthalmology and Eye Diseases , 2012,
Abstract: Background: Fetal surgery is selectively offered for severe or life-threatening fetal malformations. These infants are often born prematurely and are thus at risk for retinopathy of prematurity (ROP). It is not known whether fetal surgery confers an increased risk of developing severe ROP relative to published rates in standard premature populations ≤37 weeks of age grouped by birth weight (<1500 grams or ≥1500 grams). Design: This is a retrospective chart review. Methods: We reviewed the charts of 137 patients who underwent open fetal/fetoscopic surgery from 1996–2004. Surgical indications included twin-twin transfusion syndrome (TTTS), myelomeningocele (MMC), congenital diaphragmatic hernia (CDH), sacrococcygeal teratoma (SCT), cystic adenomatoid malformation of the lung (CCAM), and twin reversed arterial perfusion sequence (TRAP). Of these, 17 patients had local ROP examination data. Binomial tests were performed to assess whether rates of ROP in our fetal/fetoscopic surgery cohort were significantly different from published rates. Results: There were 5 patients each with an underlying diagnosis of TTTS and MMC, 2 patients each with CDH and TRAP, and 1 patient each with SCT, CCAM, and mediastinal teratoma. The mean gestational age at surgery was 234/7 ± 23/7 weeks, mean gestational age at birth was 30 ± 25/7 weeks, and mean birth weight was 1449 ± 510 grams (610–2485). Compared to published rates of ROP and threshold ROP, our fetal surgery patients had significantly higher rates of ROP and threshold ROP in both the <1500 grams and the ≥1500 grams group (all p-values < 0.05). Conclusions: Fetal/fetoscopic surgery appears to significantly increase the rate of ROP and threshold ROP development. Greater numbers are needed to confirm these observations.
Microarray profile of differentially expressed genes in a monkey model of allergic asthma
Jun Zou, Simon Young, Feng Zhu, Ferdous Gheyas, Susan Skeans, Yuntao Wan, Luquan Wang, Wei Ding, Motasim Billah, Terri McClanahan, Robert L Coffman, Robert Egan, Shelby Umland
Genome Biology , 2002, DOI: 10.1186/gb-2002-3-5-research0020
Abstract: Of the approximately 40,000 cDNAs represented on the microarray, expression levels of 169 changed by more than 2.5-fold in at least one of the pairwise probe comparisons; these cDNAs encoded 149 genes, of which two thirds are known genes. The largest number of regulated genes was observed 4 h after challenge. Confirmation of differential expression in the original tissue was obtained for 95% of a set of these genes using real-time PCR. Cluster analysis revealed at least five groups of genes with unique expression patterns. One cluster contained genes for several chemokine mediators including eotaxin, PARC, MCP-1 and MCP-3. Genes involved in tissue remodeling and antioxidant responses were also identified as regulated by antigen and IL-4 or by antigen only.This study provides a large-scale profile of gene expression in the primate lung following allergen or IL-4 challenge. It shows that microarrays, with real-time PCR, are a powerful tool for identifying and validating differentially expressed genes in a disease model.Asthma is one of the most serious allergic diseases. Characteristic features of atopic asthma are circulating specific IgE antibodies, positive skin tests to common allergens, and infiltration of the bronchial mucosa with eosinophils and Th2 cells. The resulting pulmonary inflammation leads to bronchoconstriction, airway hyper-responsiveness, and ultimately to airway remodeling [1]. Many cellular mediators, including cytokines and chemokines, are involved in asthma; Th2-type cytokines such as interleukin-4 (IL-4), IL-5, IL-9 and IL-13 may contribute to pathophysiological changes in asthma [2]. The complexity of asthma originates from the interaction of an unknown number of genes with environmental factors [3]. Studies of twins have shown that concordance rates for asthma are significantly higher in monozygotic twins than in dizygotic twins, and that the heritability of asthma may be as high as 75% [4]. Linkage analysis of asthma within families has reve
Advances in the treatment of Raynaud’s phenomenon
Terri L Levien
Vascular Health and Risk Management , 2010, DOI: http://dx.doi.org/10.2147/VHRM.S4551
Abstract: dvances in the treatment of Raynaud’s phenomenon Review (6735) Total Article Views Authors: Terri L Levien Published Date March 2010 Volume 2010:6 Pages 167 - 177 DOI: http://dx.doi.org/10.2147/VHRM.S4551 Terri L Levien Drug Information Pharmacist and Clinical Associate Professor, College of Pharmacy, Washington State University Spokane, WA, USA Abstract: Raynaud’s phenomenon is a common condition characterized by vasospasm of the digital arteries and resulting cyanosis and redness. It often does not require pharmacologic management, but in some cases symptoms are severe and pharmacologic management is necessary. Calcium channel blockers are often used first-line, but in some patients are ineffective. Patients with severe symptoms or intolerance to available therapies have prompted exploration of alternative therapies, including endothelin antagonists, phosphodiesterase-5 inhibitors, antioxidants, newer vasodilators, statins, and botulinum toxin. These newer therapies provide the focus for this review.
Discovering Features of Web-Based Algebraic Tools Via Data Analysis to Support Technology Integration in Mathematics Education
Kurz, Terri L.
Journal of Curriculum and Instruction , 2011,
Abstract: Technological tools available on the Internet can be used to support teachers’ understanding of how to teach mathematics to their students. This paper outlines a method for using algebraic tools in mathematics with teachers to help them discover features to facilitate student learning and understanding with the support of statistical software. The teachers first investigate algebraic tools and then analyze features of the tools and how they support or limit student learning. Personal Construct Theory (Kelly, 1955) is used to first help teachers create and self administer repertory grids and then generate dendrograms for cluster analysis. The model described can help others implement technology in a similar manner making use of both web-based applets and statistical software in an authentic context.
Advances in the treatment of Raynaud’s phenomenon
Terri L Levien
Vascular Health and Risk Management , 2010,
Abstract: Terri L LevienDrug Information Pharmacist and Clinical Associate Professor, College of Pharmacy, Washington State University Spokane, WA, USAAbstract: Raynaud’s phenomenon is a common condition characterized by vasospasm of the digital arteries and resulting cyanosis and redness. It often does not require pharmacologic management, but in some cases symptoms are severe and pharmacologic management is necessary. Calcium channel blockers are often used first-line, but in some patients are ineffective. Patients with severe symptoms or intolerance to available therapies have prompted exploration of alternative therapies, including endothelin antagonists, phosphodiesterase-5 inhibitors, antioxidants, newer vasodilators, statins, and botulinum toxin. These newer therapies provide the focus for this review.Keywords: Raynaud, iloprost, bosentan, phosphodiesterase-5 inhibitors
Resisting Negative Images and Stereotypes: One Latina Prospective Teacher’s Story
Terri L. Rodriguez
Brock Education : a Journal of Educational Research and Practice , 2010,
Abstract: This article focuses on one Latina prospective teacher’s act of resisting negative stereotypes regarding attire imposed upon her by a white female principal. The event is embedded within a larger life history study that explores the experiences of bilingual Latino prospective teachers in the elementary education program of a large Midwestern university. The selected narrative is contextualized in relation to resistance narratives. Patricia Morales tells about her experiences in U.S. schools. It explores how Patricia’s life history is marked by experiences of discrimination, yet how her constructions of these events represent “counterstories” (Delgado, 2000; Solorzano & Yosso, 2002) through which she “talks back” (hooks, 1989) to distorted images and stereotypes. Patricia’s narratives are shown to constitute creative acts of resistance through which she negotiates a positive and affirming identity (Suarez-Orozco & Suarez-Orozco, 2001) as a Latina prospective teacher.
Resisting Negative Images and Stereotypes: One Latina Prospective Teacher’s Story
Terri L. Rodriguez
Brock Education : a Journal of Educational Research and Practice , 2010,
Abstract: This article focuses on one Latina prospective teacher’s act of resisting negative stereotypes regarding attire imposed upon her by a white female principal. The event is embedded within a larger life history study that explores the experiences of bilingual Latino prospective teachers in the elementary education program of a large Midwestern university. The selected narrative is contextualized in relation to resistance narratives. Patricia Morales tells about her experiences in U.S. schools. It explores how Patricia’s life history is marked by experiences of discrimination, yet how her constructions of these events represent “counterstories” (Delgado, 2000; Solorzano & Yosso, 2002) through which she “talks back” (hooks, 1989) to distorted images and stereotypes. Patricia’s narratives are shown to constitute creative acts of resistance through which she negotiates a positive and affirming identity (Suarez-Orozco & Suarez-Orozco, 2001) as a Latina prospective teacher. Keywords: preservice teachers; teacher education; Latino critical race theory; narrative inquiry
Predictors of hospitalization and institutionalization in Medicaid patient populations with Alzheimer’s Disease  [PDF]
Adrienne M. Gilligan, Daniel C. Malone, Terri L. Warholak, Edward P. Armstrong
Advances in Alzheimer's Disease (AAD) , 2013, DOI: 10.4236/aad.2013.23011
Abstract: Objectives: Determine predictors of hospitalization and institutionalization in Medicaid populations with Alzheimer’s Disease (AD). Methods: Data were obtained from the Centers for Medicareand Medicaid Services (CMS). Individuals enrolled in Florida,New Jersey, and New York Medicaid programs on January 1, 2004, remained in that program for 1 year and exposed to an AD medication were included. AD diagnosis was based on the ICD-9-CM code 331.0. Outcomes of interest were hospitalization and institutionalization. Multivariate logistic regression models were used to test for the association between outcomes of interest and demographics, resource utilization factors, and type of AD pharmacotherapy exposure. Results: A total of 65,442 individuals qualified for the study. Age was positively and significantly associated with hospitalization (p < 0.001) and institutionalization (p < 0.001). Exposure tomemantine was significantly associated with less chance of hospitalization (OR = 0.88; 99% CI: 0.77 - 0.99). Non-Hispanic Blacks were significantly (p < 0.001) more likely to be hospitalized than Non-Hispanic Whites (OR = 1.60; 99% CI: 1.41 - 1.81). Compared to Non-Hispanic Whites, Non-Hispanic Blacks (OR = 0.73; 99% CI: 0.60 - 0.88), Hispanics (OR = 0.36; 99% CI: 0.27 - 0.47), and Non-Hispanic Others (OR = 0.42; 99% CI: 0.21 - 0.82) were significantly less likely to be institutionalized. Individuals prescribed donepezil (OR = 1.29, 99% CI, 1.08 - 1.54) and galantamine (OR = 1.46, 99% CI: 1.19 - 1.79) were significantly more likely to have an institutionalization claim (p < 0.001 for both medications). Residents of New York were significantly morelikely to be hospitalized than Florida residents (OR = 1.30; 99% CI: 1.17 - 1.44), where as New Jersey residents were significantly less likely to be hospitalized (OR = 0.75; 99% CI: 0.66 - 0.85). Finally, compared toFloridaresidents, residents of New Jerseywere significantly more likely to be institutionalized (OR = 4.61; 99% CI: 3.98 - 5.33). Conclusion: Demographics, state of residence and pharmacotherapy exposure weresignificant predictors of health care service utilization. Further pharmacoeconomic studies in AD medication therapy are warranted.
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