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Search Results: 1 - 10 of 76388 matches for " Tania Maria Pacheco Schubach "
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Retrospective study of 151 patients with cutaneous leishmaniasis treated with meglumine antimoniate
Schubach Armando de Oliveira,Marzochi Keyla B. Feldman,Moreira Jo?o Soares,Schubach Tania Maria Pacheco
Revista da Sociedade Brasileira de Medicina Tropical , 2005,
Abstract: We retrospectively analyzed a series of 151 cases of cutaneous leishmaniasis treated between 1967 and 1982. One-hundred-and-thirty-nine (92%) patients presented with active lesions and were treated with daily doses of meglumine antimoniate: 81 adults received a 5-ml vial IM and 58 children received 1 to 5ml. Forty-five (32.4%) patients underwent continuous treatment with meglumine antimoniate for 25 to 116 days without rest intervals, and 94 (67.6%) intermittent treatment with 2 to 5 series of meglumine antimoniate. Intermittent series could include schedules of daily IM applications for 10 to 25 days each and intervals varying from 10 to 60 days. Antimony dose was calculated for 66 (47.5%) patients and ranged from 3.9 to 28.7 Sb5+/kg/day. Of these, 35 patients received >10mg and 31 patients <10mg Sb5+/kg/day. Median time of healing was longer for lesions on the legs and feet - 67.5 days versus 48.7 days (p < 0.001) for other sites. However, there were no significant differences in the median time of healing between adults and children, intermittent and continuous regimens or high and low antimony doses. Fifty-one patients were reassessed 5 to 14 years after treatment and showed no evidence of disease. These results support further investigation (clinical trials) on treatment using low doses of antimony.
Sporotrichosis: an emergent zoonosis in Rio de Janeiro
Barros M?nica Bastos de Lima,Schubach Tania Maria Pacheco,Gutierrez Galhardo Maria Clara,Schubach Armando de Oliveira
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: During the period from 1987 to 1998, 13 cases of human sporotrichosis were recorded at the Research Center Evandro Chagas Hospital (CPqHEC) in Rio de Janeiro. Two of these patients related scratch by a sick cat. During the subsequent period from July 1998 to July 2000, 66 human, 117 cats and 7 dogs with sporotrichosis were diagnosed at the CPqHEC. Fifty-two humans (78.8%) reported contact with cats with sporotrichosis, and 31 (47%) of them reporting a history of a scratch or bite. This epidemic, unprecedented in the literature, involving cats, dogs and human beings may have started insidiously before 1998.
Detection of poxvirus in cattle associated with human cases in the State of Rio de Janeiro: preliminary report
Schatzmayr, Hermann Gon?alves;Lemos, Elba Regina Sampaio de;Mazur, Carlos;Schubach, Armando;Majerowicz, Selma;Rozental, Tatiana;Schubach, Tania Maria Pacheco;Bustamante, Maria Cristina;Barth, Ortrud Monika;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000500007
Abstract: this preliminary report describes human and cow cases of poxvirus that recently ocurred in the state of rio de janeiro. the electron microscopic findings were consistent with parapoxviral and orthopoxviral infection. orthopoxvirus strains were isolated from human and cow cases. detailed viral characterization by means of genetical techniques is under investigation. based on these informations, poxviral diseases should be also considered an emerging viral zoonosis that can affect human beings.
Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the Municipality of Rio de Janeiro, Brazil
Madeira, Maria de Fátima;Schubach, Armando de Oliveira;Schubach, Tania Maria Pacheco;Leal, Cristianni Antunes;Marzochi, Mauro Célio de Almeida;
Brazilian Journal of Infectious Diseases , 2004, DOI: 10.1590/S1413-86702004000600008
Abstract: euthanasia of seropositive dogs has been one of the principal measures adopted by the program for the control of leishmaniasis in brazil for many years. however, its efficacy is currently being questioned. we obtained intact skin samples from 20 leishmania-reactive dogs from the municipality of rio de janeiro that had been referred for euthanasia. the promastigote forms of leishmania were isolated in culture from 18 of these animals. fourteen of these isolates were identified as leishmania (leishmania) chagasi by isoenzyme electrophoresis; seven of these were from asymptomatic dogs and seven were from symptomatic animals with visceral leishmaniasis. in conclusion, cutaneous parasitism is found in the intact skin of dogs naturally infected with l. (l.) chagasi, irrespective of the presence or absence of clinical signs suggestive of visceral leishmaniasis.
Retrospective study of 151 patients with cutaneous leishmaniasis treated with meglumine antimoniate
Schubach, Armando de Oliveira;Marzochi, Keyla B. Feldman;Moreira, Jo?o Soares;Schubach, Tania Maria Pacheco;Araújo, Marcelo Lodi;Vale, Ant?nio Carlos Francesconi do;Passos, Sonia Regina Lambert;Marzochi, Mauro Célio de Almeida;
Revista da Sociedade Brasileira de Medicina Tropical , 2005, DOI: 10.1590/S0037-86822005000300001
Abstract: we retrospectively analyzed a series of 151 cases of cutaneous leishmaniasis treated between 1967 and 1982. one-hundred-and-thirty-nine (92%) patients presented with active lesions and were treated with daily doses of meglumine antimoniate: 81 adults received a 5-ml vial im and 58 children received 1 to 5ml. forty-five (32.4%) patients underwent continuous treatment with meglumine antimoniate for 25 to 116 days without rest intervals, and 94 (67.6%) intermittent treatment with 2 to 5 series of meglumine antimoniate. intermittent series could include schedules of daily im applications for 10 to 25 days each and intervals varying from 10 to 60 days. antimony dose was calculated for 66 (47.5%) patients and ranged from 3.9 to 28.7 sb5+/kg/day. of these, 35 patients received >10mg and 31 patients <10mg sb5+/kg/day. median time of healing was longer for lesions on the legs and feet - 67.5 days versus 48.7 days (p < 0.001) for other sites. however, there were no significant differences in the median time of healing between adults and children, intermittent and continuous regimens or high and low antimony doses. fifty-one patients were reassessed 5 to 14 years after treatment and showed no evidence of disease. these results support further investigation (clinical trials) on treatment using low doses of antimony.
Sporotrichosis: an emergent zoonosis in Rio de Janeiro
Barros, M?nica Bastos de Lima;Schubach, Tania Maria Pacheco;Gutierrez Galhardo, Maria Clara;Schubach, Armando de Oliveira;Monteiro, Paulo Cezar Fialho;Reis, Rosani Santos;Zancopé-Oliveira, Rosely Maria;Lazéra, Márcia dos Santos;Cuzzi-Maya, Tullia;Blanco, Tania Cristina Moita;Marzochi, Keyla Belizia Feldman;Wanke, Bodo;Valle, Antonio Carlos Francesconi do;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000600006
Abstract: during the period from 1987 to 1998, 13 cases of human sporotrichosis were recorded at the research center evandro chagas hospital (cpqhec) in rio de janeiro. two of these patients related scratch by a sick cat. during the subsequent period from july 1998 to july 2000, 66 human, 117 cats and 7 dogs with sporotrichosis were diagnosed at the cpqhec. fifty-two humans (78.8%) reported contact with cats with sporotrichosis, and 31 (47%) of them reporting a history of a scratch or bite. this epidemic, unprecedented in the literature, involving cats, dogs and human beings may have started insidiously before 1998.
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
Reis, Rosani Santos;Almeida-Paes, Rodrigo;Muniz, Mauro de Medeiros;Tavares, Patrícia Morais e Silva;Monteiro, Paulo Cezar Fialho;Schubach, Tania Maria Pacheco;Gutierrez-Galhardo, Maria Clara;Zancopé-Oliveira, Rosely Maria;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000500018
Abstract: an epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus sporothrix schenckii, is ongoing in rio de janeiro, brazil, in which cases of human infection are related to exposure to cats. in an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by dna-based typing methods 19 human and 25 animal s. schenckii isolates from the epidemic, as well as two control strains. to analyse the isolates, the random amplified polymorphic dna (rapd) technique was performed using three different primers, together with dna fingerprinting using the minisatellite derived from the wild-type phage m13 core-sequence. the analyses generated amplicons with considerable polymorphism. although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. the rapd profiles of epidemic s. schenckii isolates could be distinguished from that of the united states isolate, displaying 20% similarity to each primer and 60% when amplified with the m13 primer. dna fingerprinting of s. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. it is clear that cats act as a vehicle for dissemination of s. schenckii.
Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks
Anderson Messias Rodrigues,Marcus de Melo Teixeira,G. Sybren de Hoog,Tania Maria Pacheco Schubach,Sandro Antonio Pereira,Geisa Ferreira Fernandes,Leila Maria Lopes Bezerra,Maria Sueli Felipe,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002281
Abstract: Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), S?o Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens
Anderson Messias Rodrigues?,Geisa Ferreira Fernandes?,Leticia Mendes Araujo?,Paula Portella Della Terra?,Priscila Oliveira dos Santos?,Sandro Antonio Pereira?,Tania Maria Pacheco Schubach,Eva Burger?,Leila Maria Lopes-Bezerra?,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004016
Abstract: Background Sporothrix schenckii and associated species are agents of human and animal sporotrichosis that cause large sapronoses and zoonoses worldwide. Epidemiological surveillance has highlighted an overwhelming occurrence of the highly pathogenic fungus Sporothrix brasiliensis during feline outbreaks, leading to massive transmissions to humans. Early diagnosis of feline sporotrichosis by demonstrating the presence of a surrogate marker of infection can have a key role for selecting appropriate disease control measures and minimizing zoonotic transmission to humans. Methodology We explored the presence and diversity of serum antibodies (IgG) specific against Sporothrix antigens in cats with sporotrichosis and evaluated the utility of these antibodies for serodiagnosis. Antigen profiling included protein extracts from the closest known relatives S. brasiliensis and S. schenckii. Enzyme-linked immunosorbent assays and immunoblotting enabled us to characterize the major antigens of feline sporotrichosis from sera from cats with sporotrichosis (n = 49), healthy cats (n = 19), and cats with other diseases (n = 20). Principal Findings Enzyme-linked immunosorbent assay-based quantitation of anti-Sporothrix IgG exhibited high sensitivity and specificity in cats with sporotrichosis (area under the curve, 1.0; 95% confidence interval, 0.94–1; P<0.0001) versus controls. The two sets of Sporothrix antigens were remarkably cross-reactive, supporting the hypothesis that antigenic epitopes may be conserved among closely related agents. One-dimensional immunoblotting indicated that 3-carboxymuconate cyclase (a 60-kDa protein in S. brasiliensis and a 70-kDa protein in S. schenckii) is the immunodominant antigen in feline sporotrichosis. Two-dimensional immunoblotting revealed six IgG-reactive isoforms of gp60 in the S. brasiliensis proteome, similar to the humoral response found in human sporotrichosis. Conclusions A convergent IgG-response in various hosts (mice, cats, and humans) has important implications for our understanding of the coevolution of Sporothrix and its warm-blooded hosts. We propose that 3-carboxymuconate cyclase has potential for the serological diagnosis of sporotrichosis and as target for the development of an effective multi-species vaccine against sporotrichosis in animals and humans.
Use of elisa employing homologous and heterologous antigens for the detection of IgG and subclasses (IgG1 and IgG2) in the diagnosis of Canine visceral leishmaniasis
Ribeiro, Flávia Coelho;Schubach, Armando de O.;Mouta-Confort, Eliame;Pacheco, Tania M.V.;Madeira, Maria de Fátima;Abboud, Luiz Cláudio de Souza;Honse, Carla de Oliveira;Alves, Andreia Silva;Marzochi, Mauro C.A.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2011, DOI: 10.1590/S0036-46652011000500008
Abstract: indirect immunofluorescence is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is limited. since elisa does not present these limitations, this technique might be an option for the detection of igg or specific igg1 and igg2 subclasses. canine ehrlichiosis is an important differential diagnosis of american visceral leishmaniasis (avl). the present study compared elisa using leishmania chagasi and leishmania braziliensis antigen for the detection of anti-leishmania igg and subclasses in serum samples from 37 dogs naturally infected with l. chagasi (avl) and in samples from four dogs co-infected with l. braziliensis and l. chagasi (ci). the occurrence of cross-reactivity was investigated in control serum samples of 17 healthy dogs (hc) and 35 infected with ehrlichia canis (ec). the mean optical density obtained for the detection of igg was significantly higher when l. chagasi antigen was used, and was also higher in subgroup vls (symptomatic) compared to subgroup vla (asymptomatic). the correlation between igg and igg1 was low. the present results suggest that igg elisa using homologous antigen yields the best results, permitting the diagnosis of asymptomatic l. chagasi infection and the discrimination between cases of avl and ehrlichiosis in dogs.
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