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Search Results: 1 - 3 of 3 matches for " Tambarussi "
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In silico analysis of Simple Sequence Repeats from chloroplast genomes of Solanaceae species
Evandro Vagner Tambarussi,Danila Montewka Melotto-Passarin,Simone Guidetti Gonzalez,Joice Bissoloti Brigati
Crop Breeding and Applied Biotechnology , 2009,
Abstract: The availability of chloroplast genome (cpDNA) sequences of Atropa belladonna, Nicotiana sylvestris, N.tabacum, N. tomentosiformis, Solanum bulbocastanum, S. lycopersicum and S. tuberosum, which are Solanaceae species,allowed us to analyze the organization of cpSSRs in their genic and intergenic regions. In general, the number of cpSSRs incpDNA ranged from 161 in S. tuberosum to 226 in N. tabacum, and the number of intergenic cpSSRs was higher than geniccpSSRs. The mononucleotide repeats were the most frequent in studied species, but we also identified di-, tri-, tetra-, pentaandhexanucleotide repeats. Multiple alignments of all cpSSRs sequences from Solanaceae species made the identification ofnucleotide variability possible and the phylogeny was estimated by maximum parsimony. Our study showed that the plastomedatabase can be exploited for phylogenetic analysis and biotechnological approaches.
Estrutura genética de popula??es de melhoramento de Pinus caribaea var. Hondurensis por meio de marcadores microssatélites
Furlan, Rodrigo de Andrade;Mori, Edson Seizo;Tambarussi, Evandro Vagner;Moraes, Cristiano Bueno de;Jesus, Frederico Almeida de;Zimback, Léo;
Bragantia , 2007, DOI: 10.1590/S0006-87052007000400004
Abstract: the pinus caribaea var. hondurensis barret & golfari is highly important as a cultivated species for wooden production and approximately 1.8 million hectare are planted with the species, in brazil. this research has aimed to verify through ssr markers the genetic variability in pinus caribaea var. hondurensis, as well as its maintenance during genetic improvement, into the base population for breeding, a population of elite trees and an f1 hybrid population. for this study we have transferred primers of ssr loci developed for other pinus species. eight out of 20 tested primers have been successfully transferred to the p. caribaea (rps 25b, rps 150, psm 2, pr 4.6, pttx 2037, pttx 3029, rptest 01, and rptest 09). inbreeding was verified within and between populations, and the highest observed value between populations was fst = 0.0213. the observed heterozigosity (ho) and the expected heterozigosity (he) means were, respectively, 0.2469 and 0.2489. the highest genetic distance (d = 0.0119) was observed between the base populations and the f1 improved population. based on genetic distance values obtained for elite trees, 10 potential crossings between contrasting elite trees have been indicated, aiming to reach the hybrid vigor between progenies from those crossings.
Estimative of genetic parameters in progeny test of Pinus caribaea Morelet var. hondurensis Barret & Golfari by quantitative traits and microsatellite markers
Tambarussi, Evandro Vagner;Sebbenn, Alexandre Magno;Moraes, Mário Luiz Teixeira de;Zimback, Léo;Palomino, Edwin Camacho;mori, Edson seizo;
Bragantia , 2010, DOI: 10.1590/S0006-87052010000100006
Abstract: the aims of this work were to estimate the coefficient of relatedness within families and the genetic parameters for growth related traits in a progeny test from an open-pollinated variety of pinus caribaea morelet hondurensis barret & golfari, established in mato grosso do sul state, brazil. the experimental design was the triple 10 x 10 lattice, with 96 families, three replicates, and ten plants per plot. fourteen years after planting, the trial was measured for the following traits: total height, diameter at breast height (dbh), and true volume. the estimation of coefficients of relatedness within family from microsatellite loci indicated that families are true half-sibs (^rxy = 0.253). thus, the additive genetic variance (σ2a) can be estimated assuming that the genetic variance among progenies (σ2p) accounts for ? of additive genetic variance (^σ2a = 4^σ2p). the estimative of heritability coefficients at individual level (hi2) was relatively high (0.28 for dbh and 0.44 for height). the heritability coefficient considering the average families (hm2) was also high, ranging among the traits from 0.50 to 0.58. these results suggest that the population can be improved by both massal and among families selection. additionally, the estimated genetic gains with sequential selection among and within families were high (ranging from 8.92% for height to 37.56% for volume), demonstrating that this method of selection can generate high genetic improvement.
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