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Prevalência das altera??es fonológicas e uso dos processos fonológicos em escolares aos 7 anos
Patah, Luciane Kalil;Takiuchi, Noemi;
Revista CEFAC , 2008, DOI: 10.1590/S1516-18462008000200004
Abstract: purpose: to check the prevalence of phonological acquisition disorders in relation to gender and age and to identify the phonological process most used by scholars with speech therapy disorders. methods: analyzing records of screenings applied in 1076 fundamental first year students, aged 7;0-7;11-year old, divided in 4 groups according to age groups. results: the founded prevalence was 8.27%. in the group of disordered children, 77% were boys. comparing groups according to age, the disorders index varied from 6.99% to 10.11%, without significant statistical differences. the five phonological processes most used by disordered children were, in decreasing order: cluster reduction (60.67%), liquids reduction (47.19%), fricatives devoicing (22.47%), plosives devoicing (19.1%) and deletion of final consonants (11.53%). there were differences in relation to process usage, comparing genders. conclusion: the high prevalence of phonological disorders in 7-year old children suggests that prevention programs are required in earlier ages. the majority of disordered children were boys, with statistical significance. comparing age groups, the disorders rate did not decrease with increase of age groups. there were differences between genders not only in relation to prevalence but also in relation to process usage, being devoicing processes more frequent in females.
Molecular analysis of the bovine coronavirus S1 gene by direct sequencing of diarrheic fecal specimens
Takiuchi, E.;Alfieri, A.F.;Alfieri, A.A.;
Brazilian Journal of Medical and Biological Research , 2008, DOI: 10.1590/S0100-879X2008000400004
Abstract: bovine coronavirus (bcov) causes severe diarrhea in newborn calves, is associated with winter dysentery in adult cattle and respiratory infections in calves and feedlot cattle. the bcov s protein plays a fundamental role in viral attachment and entry into the host cell, and is cleaved into two subunits termed s1 (amino terminal) and s2 (carboxy terminal). the present study describes a strategy for the sequencing of the bcov s1 gene directly from fecal diarrheic specimens that were previously identified as bcov positive by rt-pcr assay for n gene detection. a consensus sequence of 2681 nucleotides was obtained through direct sequencing of seven overlapping pcr fragments of the s gene. the samples did not undergo cell culture passage prior to pcr amplification and sequencing. the structural analysis was based on the genomic differences between brazilian strains and other known bcov from different geographical regions. the phylogenetic analysis of the entire s1 gene showed that the bcov brazilian strains were more distant from the mebus strain (97.8% identity for nucleotides and 96.8% identity for amino acids) and more similar to the bcov-ent strain (98.7% for nucleotides and 98.7% for amino acids). based on the phylogenetic analysis of the hypervariable region of the s1 subunit, these strains clustered with the american (bcov-ent, 182ns) and canadian (bcq20, bcq2070, bcq9, bcq571, bcq1523) calf diarrhea and the canadian winter dysentery (bcq7373, bcq2590) strains, but clustered on a separate branch of the korean and respiratory bcov strains. the bcov strains of the present study were not clustered in the same branch of previously published brazilian strains (ay606193, ay606194). these data agree with the genealogical construction and suggest that at least two different bcov strains are circulating in brazil.
Utiliza??o e compreens?o de preposi??es por crian?as com distúrbio específico de linguagem
Puglisi, Marina Leite;Befi-Lopes, Debora Maria;Takiuchi, Noemi;
Pró-Fono Revista de Atualiza??o Científica , 2005, DOI: 10.1590/S0104-56872005000300007
Abstract: background: the ability of children with specific language impairment (sli) to use and comprehend closed-class words. aim: acknowledging the increasing search for the characterization of linguistic performance of this population in different languages, as well as the findings of international literature that stress the difficulty of these children in using closed-class grammatical morphemes, the aim of this study was to analyze the performance of children with sli in tests of production and comprehension of prepositions. the purpose of this research was also to compare the performance of these children to that of their typical language developing peers, in terms of the overall index of correct answers and the most frequent types of errors in both groups. method: an objective test was developed in order to analyze production and comprehension of four types of prepositions that are used to establish the following semantic relations: with (company relation); by (way relation); in, on or at (place/position relations); and to (destination relation). this test was carried out with 19 sli children and 29 typically developing children (cg - control group), one year younger than the research group. results: sli children presented the same pattern of results, although delayed, of children in the cg in the comprehension test. in the task involving production, however, the performance of sli children was quantitatively and qualitatively inferior to their typical developing controls. this performance was mainly characterized by the omission of prepositions. conclusion: the findings corroborate the view that grammatical words constitute a challenge for sli children, since they presented many errors that suggest difficulties in the domain of prepositions. the results also emphasized, for the sli group, deficits in the expressive language.
Bovine herpervirus type 1: infection and diagnosis methods/ Herpesvírus bovino tipo 1: Tópicos sobre a infec o e métodos de diagnóstico
Elisabete Takiuchi,Alice Fernandes Alfieri,Amauri Alcindo Alfieri
Semina : Ciências Agrárias , 2001,
Abstract: Bovine herpervirus type 1 (BHV-1) is widespread in beef and dairy herds, causing great economic losses in cattle breeding. Infections caused by BHV-1 involving the respiratory, genital, reproductive and nervous systems can determine a wide variety of clinical manifestations wich are common on the other infectious and parasitic diseases. Several methodologies have been developed for laboratory diagnosis of BHV-1. Traditional serological techniques are unable to diferentiate animals exposed to the virus strains in the vaccine from those seropositives by natural exposure to the field strains. Thus the diagnosis can only be conclusive by detecting BHV-1 directly in clinical samples. Virus isolation has the disadvantage of requering viability in the viral particle, which in many situations may reduce the sensitivity and specificity of the technique. The immunoperoxidase and immunofluorescent techniques, although they do not require the infectiousness of the virus, can be seriously compromised if the structural integrity of the viral particle is not maintained. Currently the polymerase chain reaction assay is being universally adopted in the diagnosis of many virus, including BHV-1. This methodology has the advantage of not requiring the viral particle viability while providing high sensitivity and specificity and fast results. O herpesvírus bovino tipo 1 (BHV-1) encontra-se amplamente disseminado em rebanhos de corte e leite, determinando grandes prejuízos econ micos à explora o pecuária. A infec o pelo BHV-1 pode comprometer os tratos respiratório, genital, reprodutivo e nervoso e ocasionar uma ampla variedade de manifesta es clínicas que s o comuns a outras doen as infecto-parasitárias, inviabilizando o diagnóstico clínico conclusivo. Várias metodologias foram desenvolvidas para a realiza o do diagnóstico laboratorial do BHV-1. Contudo, as técnicas sorológicas tradicionais s o incapazes de diferenciar a soroconvers o originada pela infec o natural daquela devido à vacina o. Com isto, o diagnóstico conclusivo somente é possível pela identifica o do BHV-1 diretamente em amostras clínicas. O isolamento viral em cultivo celular apresenta como desvantagem a necessidade da viabilidade da partícula viral, o que em muitas situa es pode ocasionar uma redu o na sensibilidade e especificidade da técnica. As técnicas de imunoperoxidase e imunofluorescência, apesar de n o exigirem a infecciosidade do vírus, podem ser seriamente comprometidas caso a integridade estrutural da partícula n o seja mantida. Atualmente a técnica da rea o em cadeia pela polimerase es
An outbreak of winter dysentery caused by bovine coronavirus in a high-production dairy cattle herd from a tropical country
Takiuchi, Elisabete;Barry, Aline Fernandes;Alfieri, Alice Fernandes;Filippsen, Patrícia;Alfieri, Amauri Alcindo;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700008
Abstract: bovine coronavirus (bcov) is a known cause of winter dysentery (wd) in adult cattle. the morbidity of the disease is high, that results in a significant decrease in milk production and consequently, economic losses. in the present study, we report on a classical outbreak of wd that affected a high-production holstein dairy herd raised in a tropical country. the lactating batch included 154 cows, and 138 (90%) presented diarrhea in a short (nine days) period of time. three (2%) cows died. the other batches of animals did not become ill. the evolution of the disease in the herd, including the clinical signs and epidemiological features, strongly suggested a wd case. semi-nested pcr and rflp confirmed that bcov was the cause of the infection. samples tested negative for all other enteric pathogens. this case report highlights the importance of bcov in wd even in tropical countries such as brazil.
Identification of a mutation in the spike protein cleavage site in Brazilian strains of wild-type bovine coronavirus
Takiuchi, Elisabete;Barreiros, Marco Ant?nio Bacellar;Alfieri, Alice Fernandes;Alfieri, Amauri Alcindo;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000400021
Abstract: the spike (s) protein of coronaviruses, a type i membrane glycoprotein, is primarily responsible for entry into susceptible cells by binding with specific receptors on cells and mediating subsequent virus-cell fusion. the bovine coronavirus (bcov) s protein is cleaved into two subunits, the n-terminal s1 and the c-terminal s2. the proteolytic cleavage site of s protein is highly conserved among bcov strains and is located between amino acids 763 and 768 (krrsrr). this study describes a single mutation in the s protein cleavage site of three brazilian strains of bcov detected in diarrheic fecal samples from calves naturally infected. the sequenced pcr products revealed that amino acid sequence of the cleavage site of our strains was krrssr, indicating a mutation at amino acid position 767 (r ? s). this amino acid substitution occurred due to a single nucleotide substitution in the sequence of dna corresponding to the proteolytic cleavage site, cgt to agt. this is the first description of this nucleotide mutation (c to a), which resulted in the substitution of arginine to serine in the s cleavage site. in this study we speculated the probable effects of this mutation in the proteolytic cleavage site using the murine hepatitis coronavirus (mhv) as a comparative model.
Memória de curto-prazo visual em crian?as com distúrbio específico de linguagem
Menezes, Camila Gioconda de Lima e;Takiuchi, Noemi;Befi-Lopes, Debora Maria;
Pró-Fono Revista de Atualiza??o Científica , 2007, DOI: 10.1590/S0104-56872007000400007
Abstract: background: relationship between specific language impairment (sli) and visual short-term memory. aim: to compare the performance of children with sli to a control group of children with normal language development in tasks involving visual short-term memory. method: subjects were 20 sli children (ages 3;0 to 5;11), and 29 children with normal language development (ages 2;0 to 4;11), assessed in tasks of visual short-term memory involving picture recognition and localization recall of objects previously manipulated by the examiner. as the diagnosis of sli implies in the linguistic age being at least one year below the expected for the chronological age, the control group was also constituted by younger children with normal language development. results: sli children presented an inferior performance when compared to their pairs of the same age, similar to the younger children or below the younger age group. conclusion: sli children presented deficits in tasks involving visual short-term memory, which must be discussed in order to understand the nature of the disorder and also in terms of speech-language intervention.
Efetividade de um treinamento de professores de uma escola de educa??o especial usando os princípios dos métodos Hanen e V.O.E.: veja, ou?a e espere
Dall' Aqua, Maria de Fátima;Takiuchi, Noemi;Zorzi, Jaime Luiz;
Revista CEFAC , 2008, DOI: 10.1590/S1516-18462008000400003
Abstract: purpose: to check the effectiveness of a training program for teachers of students with special education needs based on hanen and s.w.l. (see, wait and listen) programs, both of them proposing an interacionist intervention line. methods: hanen program was created in 1977 by ayala manolson, a speech language pathologist in canada. it is designed for parents of children diagnosed with limited communication ability. the s.w.l program was derived from the hanen program and adapted to the portuguese language by rigoletto and monteiro. its purpose is to prepare families to stimulate their children by more effective social interactions. for the present paper, the principles of both programs were adapted to the school context. four teachers took part in this program, which consisted of ten guidance sessions and three video recordings of the teachers and their groups. each teacher was recoded on film for three times: first before the first guidance session; second during the training; and third after the guidance sessions were over. after each video recording, the speech language pathologist in charge filled out a specific form in order to assess the effectiveness of the given guidance. results: the number of communicative behaviors concerning the teachers increased significantly after developing the course. this was probably due to the given guidance and the reflections after watching the videos, when the teachers could evaluate their students and their own communication behavior, and because of the opportunity for sharing experiences with other teachers. conclusions: the intervention proposed by the programs caused some teachers to change their way of working in classroom, as they noted the value achieved in playful activities and speech and language stimulation that thy propitiate.
Púrpura em paciente com estrongiloidíase disseminada
Ribeiro Luciano C.,Rodrigues Junior Edson N.A.,Silva Margareth D.,Takiuchi Arley
Revista da Sociedade Brasileira de Medicina Tropical , 2005,
Abstract: A infec o pelo Strongyloides stercoralis em associa o com imunosupress o pode manifestar-se com les es em múltiplos órg os e sistemas, caracterizando a forma disseminada da doen a. Les es cutaneas n o s o freqüentemente relatadas e, se presentes, manifestam-se como rash e petéquias. Púrpuras bem definidas s o pouco descritas. No presente trabalho é descrito um caso de estrongiloidíase disseminada, com acometimento cutaneo em forma de púrpura, que se desenvolveu em um paciente timectomizado e usuário cr nico de corticosteróide devido à miastenia gravis.
Standardization of a polymerase chain reaction (Semi Nested–PCR) to detect bovine herpesvirus type 1 in aborted fetus and semen from naturally infected cattle/ Otimiza o da rea o em cadeia pela polimerase (Semi Nested-PCR) para a detec o do herpesvírus bovino tipo 1 em fragmentos de órg os fetais e em sêmen de bovinos naturalmente infectados
Elisabete Takiuchi,Kerlei Cristina Médici,Fernandes Alfieri,Amauri Alcindo Alfieri
Semina : Ciências Agrárias , 2003,
Abstract: The glycoprotein D gene of bovine herpesvirus type 1 (BHV-1) was detected in clinical samples from naturally infected cattle by semi-nested polymerase chain reaction (SN-PCR). Different protocols were tested to increase the sensitivity and specificity of the technique. An association of DNA extraction methods using phenol/chloroform/isoamyl alcohol followed by silica/guanidine isothiocyanate yield greater concentration and quality of amplified DNA. After optimization of primers and reaction conditions, the genome of BHV-1 (Los Angeles strain) was detected by SN-PCR in tissue culture supernatant and artificially infected semen at the 1 and 0.1 TCID50 limit, respectively. When used on clinical specimens from naturally infected cattle, the SN-PCR yield positive results in semen of seropositive bull and in organ fragments of aborted cattle fetus. The SN-PCR was a viable alternative, which was faster, sensitive, specific and less laborious to be used in the routine diagnosis of BHV-1 infection and semen health monitoring. A rea o em cadeia pela polimerase (PCR) foi empregada para a detec o parcial do gene da glicoproteína D do herpesvírus bovino tipo 1 (BHV-1) em material biológico proveniente de bovinos naturalmente infectados. Para o aumento da sensibilidade e da especificidade da PCR, foram avaliados diferentes protocolos. Para a extra o do DNA, a associa o dos métodos fenol/clorofórmio/álcool isoamílico e sílica/tiocianato de guanidina, proporcionou a amplifica o do DNA em maior concentra o e qualidade. Após a otimiza o dos primers e condi es da rea o foi possível detectar, por meio da Semi Nested-PCR (SN-PCR), o genoma da estirpe Los Angeles do BHV-1 em sobrenadante de cultura celular, sem processamento prévio, até o limite de 1 TCID50. Em sêmen artificialmente infectado o limite de detec o do BHV-1 foi de 0,1 TCID50. Quando utilizada em material biológico, proveniente de bovinos naturalmente infectados, a SN-PCR apresentou resultados positivos tanto em fragmentos de órg os de fetos bovinos abortados quanto em sêmen. Essa técnica revelou-se uma alternativa viável, rápida, sensível e específica para aplica o na rotina de diagnóstico da infec o pelo BHV-1, bem como, para o monitoramento sanitário do sêmen.
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