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Search Results: 1 - 10 of 35294 matches for " Tae-Jin Kang "
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Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants
Tae-Jin Kang, Moon-Sik Yang
BMC Biotechnology , 2004, DOI: 10.1186/1472-6750-4-20
Abstract: We developed new rapid and reliable genomic DNA extraction method. With our developed method, plant genomic DNA extraction could be performed within 30 min. The method was as follows. Plant tissue was homogenized with salt DNA extraction buffer using hand-operated homogenizer and extracted by phenol:chloroform:isoamyl alcohol (25:24:1). After centrifugation, the supernatant was directly used for DNA template for PCR, resulting in successful amplification for RAPD from various sources of plants and specific foreign genes from transgenic plants. After precipitating the supernatant, the DNA was completely digested by restriction enzymes.This DNA extraction procedure promises simplicity, speed, and efficiency, both in terms of time and the amount of plant sample required. In addition, this method does not require expensive facilities for plant genomic DNA extraction.Molecular biological studies of plants require high-quality DNA. Several DNA extraction procedures for isolating genomic DNA from various plant sources have been described, including the salt extraction method and the cetyltrimethyl ammonium bromide (CTAB) method [1] and its modifications [2,3]. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed.Most methods require the use of liquid nitrogen [4] or freeze-drying (lyophilization) [5,6] of tissue for the initial grinding, and these processes are unavailable in many regions of the world. After grinding the tissues in various extraction buffers, DNA is extracted with phenol-chloroform, or the extract is dialyzed against EDTA and a buffered Tris-HCl solution [7]. After extraction, the aqueous phase is concentrated, either by ethanol or isopropanol precipitation [8,9], or with microconcentrators (e.g., the Wizard genomic DNA purification system; Promega, USA). However, these methods are not time efficient for consistently obtaining PCR-quality DNA from calluses and plants, since they require that the tissu
In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation
Tae-Jin Oh
BMB Reports , 2012,
Abstract: Certain members of the cytochromes P450 superfamily metabolizepolyunsaturated long-chain fatty acids to several classesof oxygenated metabolites. An approach based on in silicoanalysis predicted that Streptomyces peucetius CYP107N3might be a fatty acid-metabolizing enzyme, showing highhomology with epoxidase enzymes. Homology modeling anddocking studies of CYP107N3 showed that oleic acid can fitdirectly into the active site pocket of the double bond of oleicacid within optimum distance of 4.6 from the Fe. In order toconfirm the epoxidation activity proposed by in silico analysis,a gene coding CYP107N3 was expressed in Escherichia coli.The purified CYP107N3 was shown to catalyze C9-C10epoxidation of oleic acid in vitro to 9,10-epoxy stearic acidconfirmed by ESI-MS, HPLC-MS and GC-MS spectral analysis.
Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceus
Eun-Mi Park, Jung-Ha Kang, Jung Seo, GunDo Kim, Jongkyeong Chung, Tae-Jin Choi
BMC Immunology , 2008, DOI: 10.1186/1471-2172-9-31
Abstract: A DNA fragment containing the conserved SH2 domain was amplified by RT-PCR using degenerate primers designed based on the highly conserved sequences in the SH2 domains of the zebrafish and mammalian STAT1. The complete cDNA sequence was obtained by 5' and 3' RACE. The flounder STAT1 transcript consisted of 2,909 bp that encoded a polypeptide of 749 amino acids. The overall similarity between flounder STAT1 and other STATs was very high, with the highest amino acid sequence identity to snakehead (89%). Phylogenetic analyses reveal that flounder STAT1 is in the same monophyletic group with snakehead STAT1. Quantitative real time RT-PCR and in situ hybridization revealed that STAT1 was expressed in almost all examined organs and tissues, with high expression in gill, spleen, kidney, and heart. The accumulation of STAT1 mRNA in different developmental stages, as determined by real time RT-PCR, increased with development.Recent cloning of various cytokine genes and the STAT1 gene of olive flounder here suggest that fish also use the highly specialized JAK-STAT pathway for cytokine signaling. Identification of other STAT genes will elucidate in detail the signal transduction system in this fish.Cellular responses to internal and external signals are mediated by the expression of specific genes or sets of genes, which are regulated by specific transcriptional factors [1]. Therefore, the entry of transcription factors into the nucleus is critical to their role in gene expression. Signals from cytokines and growth factors are transduced into the nucleus by the Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling pathway [2-6]. JAK-STAT signaling is also involved in the regulation of cell proliferation, differentiation, survival, motility, and apoptosis in different organs [7].The binding of signal molecules to their receptors initiates activation of JAKs, which increases their tyrosine kinase activity [4,8]. The activated JAKs phosphorylate
Tissue-specific down-regulation of RIPK 2 in Mycobacterium leprae-infected nu/nu mice
Byoung Chul Kim,Tae-Jin Kang,Song Hou Jin,Se-Kon Kim,Seong-Boem Lee,Gue-Tae Chae
Mediators of Inflammation , 2004, DOI: 10.1080/09629350410001664734
Abstract: RIPK 2 is adapter molecule in the signal pathway involved in Toll-like receptors. However, there has been no reported association between receptor-interacting serine/threonine kinase 2 (RIPK 2) expression and the infectious diseases involving mycobacterial infection. This study found that its expression was down-regulated in the footpads and skin but was up-regulated in the liver of Mycobacterium leprae-infected nu/nu mice compared with those of the M. leprae non-infected nu/nu mice. It was observed that the interlukin-12p40 and interferon-γ genes involved in the susceptibility of M. leprae were down-regulated in the skin but were up-regulated in the liver. Overall, this suggests that regulation of RIPK 2 expression is tissue-specifically associated with M. leprae infection.
Protective Effect of Tetracycline against Dermal Toxicity Induced by Jellyfish Venom
Changkeun Kang, Yeung Bae Jin, Jeongsoo Kwak, Hongseok Jung, Won Duk Yoon, Tae-Jin Yoon, Jong-Shu Kim, Euikyung Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057658
Abstract: Background Previously, we have reported that most, if not all, of the Scyphozoan jellyfish venoms contain multiple components of metalloproteinases, which apparently linked to the venom toxicity. Further, it is also well known that there is a positive correlation between the inflammatory reaction of dermal tissues and their tissue metalloproteinase activity. Based on these, the use of metalloproteinase inhibitors appears to be a promising therapeutic alternative for the treatment of jellyfish envenomation. Methodology and Principal Findings Tetracycline (a metalloproteinase inhibitor) has been examined for its activity to reduce or prevent the dermal toxicity induced by Nemopilema nomurai (Scyphozoa: Rhizostomeae) jellyfish venom (NnV) using in vitro and in vivo models. HaCaT (human keratinocyte) and NIH3T3 (mouse fibroblast) incubated with NnV showed decreases in cell viability, which is associated with the inductions of metalloproteinase-2 and -9. This result suggests that the use of metalloproteinase inhibitors, such as tetracycline, may prevent the jellyfish venom-mediated local tissue damage. In vivo experiments showed that comparing with NnV-alone treatment, tetracycline pre-mixed NnV demonstrated a significantly reduced progression of dermal toxicity upon the inoculation onto rabbit skin. Conclusions/Significance It is believed that there has been no previous report on the therapeutic agent of synthetic chemical origin for the treatment of jellyfish venom-induced dermonecrosis based on understanding its mechanism of action except the use of antivenom treatment. Furthermore, the current study, for the first time, has proposed a novel mechanism-based therapeutic intervention for skin damages caused by jellyfish stings.
Comparative analysis of pepper and tomato reveals euchromatin expansion of pepper genome caused by differential accumulation of Ty3/Gypsy-like elements
Minkyu Park, SungHwan Jo, Jin-Kyung Kwon, Jongsun Park, Jong Hwa Ahn, Seungill Kim, Yong-Hwan Lee, Tae-Jin Yang, Cheol-Goo Hur, Byoung-Cheorl Kang, Byung-Dong Kim, Doil Choi
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-85
Abstract: For sequence-level analysis, we generated 35.6 Mb of pepper genomic sequences from euchromatin enriched 1,245 pepper BAC clones. The comparative analysis of orthologous gene-rich regions between both species revealed insertion of transposons exclusively in the pepper sequences, maintaining the gene order and content. The most common type of the transposon found was the LTR retrotransposon. Phylogenetic comparison of the LTR retrotransposons revealed that two groups of Ty3/Gypsy-like elements (Tat and Athila) were overly accumulated in the pepper genome. The FISH analysis of the pepper Tat elements showed a random distribution in heterochromatic and euchromatic regions, whereas the tomato Tat elements showed heterochromatin-preferential accumulation.Compared to tomato pepper euchromatin doubled its size by differential accumulation of a specific group of Ty3/Gypsy-like elements. Our results could provide an insight on the mechanism of genome evolution in the Solanaceae family.The Solanaceae is an unusually divergent family consisting of approximately 90 genera and 3,000-4,000 species [1]. Members of the Solanaceae have evolved into extremely divergent forms, ranging from trees to annual herbs, and they occupy diverse habitats ranging from deserts to aquatic areas [1]. Such hyper-diversity in one family makes it useful to study plant adaptation and diversification. Despite this diversity, all Solanaceous species evolved during the last 40 million years [2]. Furthermore, almost all members share the same chromosome number (x = 12) [2].To date, diversity within the Solanaceae has been studied by comparative genome analyses using common genetic markers. As a result, we know that the Solanaceae genomes have undergone relatively small numbers of chromosomal rearrangements (e.g., about 5 rearrangements between potato and tomato and about 30 rearrangements between pepper and tomato), maintaining well-conserved gene content and order [3-8]. The conservation of the Solanaceae
Performance Analysis of IEEE 802.11 DCF under Nonsaturation Condition
Yutae Lee,Min Young Chung,Tae-Jin Lee
Mathematical Problems in Engineering , 2008, DOI: 10.1155/2008/574197
Abstract: Carrier sense multiple access with collision avoidance (CSMA/CA) methods are considered to be attractive MAC protocols for wireless LANs. IEEE 802.11 distributed coordination function (DCF) is a random channel access scheme based on CSMA/CA method and the binary slotted exponential backoff procedure to reduce the packet collision. In this paper, we propose a new analytical model for a nonsaturated IEEE 802.11 DCF network and evaluate its performance. We verify our model using simulations and show that our results agree with the simulations.
Multi-phase Design Optimization of a Long Range Aerial Lift Boom Structure
Henry Panganiban,In-Gyu Ahn,Tae-Jin Chung
Lecture Notes in Engineering and Computer Science , 2012,
Control of De-Lamination Phenomena in LTCC Zero-Shrinkage by Glass Infiltration Method
Dong-Hun Yeo,Tae-Jin Jo
Transactions on Electrical and Electronic Materials , 2012,
Abstract: A zero-shrinkage sintering process in which the shrinkage of x-y axis is controlled to be zero is in great demanddue to the trend of high integration in the ceramic modules. Among the zero-shrinkage sintering processes that areavailable, the proposed glass infiltration method where the viscous but fluidic glass infiltrates of the Al2O3 particles inthe structure of Al2O3/glass/Al2O3 during firing is one of the applicable methods. However, the above proposed glassinfiltration method has the problem of the warpage-like delamination. This occurred at the outermost surface of themultiple-bundle substrate. It is thought that the decomposed gas rapidly expands in low viscous glass to create vacantspace. To solve this problem, the vacant space was tamped with Al2O3 particles to lead to the actual improvement ofthe sintered properties. With 15 wt% of tamping Al2O3 particles in glass, most of the vacant space disappeared. Fullydensified zero-shrinkage substrate without delamination can be obtained.
Guest Editorial
Masato Oguchi,Hyung-Jin Choi,Tae-Jin Lee,Seong Keun Oh
Journal of Networks , 2011, DOI: 10.4304/jnw.6.3.341-342
Abstract: This special issue of the Journal of Networks consists of six selected papers from the 4th International Conference on Ubiquitous Information Management and Communication (ICUIMC 2010) held in January 14th - 15th. Among them, six qualified papers have been selected as the papers for the special issue of the Journal of Networks, which covers the recent trends in wireless communications and networking. This issue includes various research field in communications and networking technologies. As one of the recent hot topics in this field, "A Communication Protocol for Sensor Database Construction by Rounding Sink" by Tomoki Yoshihisa and Shojiro Nishio, presents a model and simulation-based analytical results in wireless sensor networks. The rounding sink approach has been presented and studied, in which rounding sinks repeatedly wander about in the area to collect data from each sensor. "An Efficient Mutual Authentication and Access Control Scheme for Wireless Sensor Networks in Healthcare" by Xuan Hung Le, Murad Khalid, Ravi Sankar, and Sungyoung Lee, also discusses an issue in wireless sensor networks. To achieve the protection of data confidentiality and patient privacy in healthcare applications using wireless sensors, a secure, lightweight public key-based security scheme is proposed and evaluated by simulation. "An Energy-efficient Multi-candidate Greedy Routing Scheme in Wireless Sensor Networks" by Dang Tu Nguyen, Wook Choi, Minh Thiep Ha, and Hyunseung Choo, introduces novel multi-candidate greedy routing scheme in wireless sensor networks. According to the simulation results, their method increases the routing performance significantly in terms of packet delivery latency and energy efficiency. A fundamental technology for wireless communications is included in this issue as well. "The Enhanced Decoding Method for QO-SFBC System in Frequency Selective Fading Channel Environment" by Young-il Min, Hwan-min Park, Jun-hee Jang, Keun-dea Kim, and Hyung-jin Choi, proposes a decoding method to improve the performance of quasi orthogonal-space frequency block coding in frequency selective fading channel environment. An analysis and evaluation results by simulation show that the method can provide better performance compared with the conventional methods. This issue also deals with the field of image processing, which can be applied in the various Internet environments. "Image Retrieval with Generative Model for Typicality" by Taro Tezuka and Akira Maeda, discusses the problem to find the most typical image that depicts the object specified by a query. Thei
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