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Search Results: 1 - 10 of 173 matches for " TITI CANDRA SUNARTI "
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Yeast Isolation for Bioethanol Production
EKA RURIANI,TITI CANDRA SUNARTI,ANJA MERYANDINI
HAYATI Journal of Biosciences , 2012,
Abstract: We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.
Isolation of Cellulolytic Bacteria and Characterization of the Enzyme
Anja Meryandini,Wahyu Widosari,Besty Maranatha,Titi Candra Sunarti
Makara Seri Sains , 2009,
Abstract: Four of cellulolitic bacteria isolates had beencharacterized. The determination of cellulase activity was conducted at the highest production time, using crudeenzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethylcellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and bananapeel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°Cand pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three.C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peelsubstrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste.However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates.
SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
TITI CANDRA SUNARTI,ANJA MERYANDINI,M. EDY SOFIYANTO,,NUR RICHANA
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2010,
Abstract: The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process. In this experiment, delignified corncob, its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three local isolates of celluloytic bacteria (C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol. The results showed that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on delignified corncob, and alpha cellulose. The highest hydrolytic activity produced from cellulose fraction was by isolate C4-4, which liberated 3.50 g/l of total sugar. Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol.
USING STREPTOMYCES XYLANASE TO PRODUCE XYLOOLIGOSACHARIDE FROM CORNCOB
ANJA MERYANDINI,TITI CANDRA SUNARTI,APRILIA NAOMI,FERY MUTIA
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2008,
Abstract: Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidifi ed method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic Acid) method. Xylanase from strain 234P-16 has the highest activity if cultivated in 1% Hawaii xylan, whereas strain SKK1-8 on 1.5% Bisma xylan. SKK1-8 xylanase can hydrolize corncob xylan (1% Hawaii or 1.5% Bisma xylan) within 4 hours and produce xylooligosacharide with polymerization degree of 4.76 and 6.37, respectively.
PENGGUNAAN XILANASE Streptomyces sp. 45 I-3 AMOBIL UNTUK HIDROLISIS XILAN TONGKOL JAGUNG [Immobilization of Extracellular Xylanase from Streptomyces sp. 45 I-3 for Hydrolysis of Corncob Xylan]
Anja Meryandini1),2)*,Titi Candra Sunarti3),Ferry Mutia3),Niken Financia Gusmawati4)
Jurnal Teknologi dan Industri Pangan , 2009,
Abstract: Xylan extraction from corncob is done by using alkaline as solvent. Xylan extraction from corncob could give the yields as 10.9%. One percent of corncob xylan is used as substrate to produce the xylanase, compared to oatspelt xylan. Immobilization of xylanase was performed using 1% EudragitTM S100 solution (w/v), with 5:1 volume ratio of xylanase and 1 % EudragitTM S100 (w/v). Activity of the immobilized xylanase was decreased to 23.97% compared with free xylanase. Immobilized xylanase have optimum pH and temperature at 6.0 and 40 C respectively, have also thermal stability at 30–40 C for an hour. Immobilized xylanase could be reused, but its activity decreased to 52.38% after 3 times application.
PERUBAHAN STRUKTUR PATI GARUT (Maranta arundinaceae) SEBAGAI AKIBAT MODIFIKASI HIDROLISIS ASAM, PEMOTONGAN TITIK PERCABANGAN DAN SIKLUS PEMANASAN-PENDINGINAN [Structure Changes of Arrowroot (Maranta arundinaceae) Starch as Influenced by Acid Hydrolysis, Debranching and Autoclaving-Cooling Cycle Modifications]
Didah Nur Faridah1)*,Dedi Fardiaz1),Nuri Andarwulan1),Titi Candra Sunarti2)
Jurnal Teknologi dan Industri Pangan , 2010,
Abstract: The effects of lintnerization (2.2 N HCl, 2 hours), debranching with pullulanase (1.3 U/g and 10.4 U/g starch) and/or three-auctoclaving-cooling cycles at 121oC for 15 minutes on the changes of arrowroot starch structures were studied. The structural modifications of amylose and amylopectin were measured by Gel Permiation Chromatography (GPC), and the distribution of degree of polimerization (DP) was analyzed by Fluorophore-Assisted Capillary Electrophoresis (FACE). The GPC profile of native starch using Toyopearl HW-65S gel gave mainly two fractions. Fraction I (Fr. I) was a high molecular weight component and Fraction II (Fr. II) was a low molecular weight component. After acid modification, the carbohydrate content of Fr. II increased while that of Fr. I decreased. The amount of DP of 6 to 8 increased in all modified arrowroot starches. The GPC and FACE analyses showed that all starch modification techniques caused the structural changes of amylopectin molecules to form short chain amyloses.
KEMBANG JEPUN : JALAN DOMINAN KOTA SURABAYA
Endang Titi Sunarti B. Darjosanjoto
Dimensi : Journal of Architecture and Built Environment , 2005,
Abstract: Configurational analysis applying space syntax technique shows that the street of Kembang Jepun is a dominant street of Surabaya. The description uses the global integration (Rn-radius infinity) map and the local integrations (R3=radius 3) map based on the map of Surabaya 2002. The measurement shown that the street of Kembang Jepun globally lays on the urban super grid of Surabaya. Locally this street not only shows the highest integration value of all streets and the connectivity value, but also its depth is shallow. By means, the street of Kembang Jepun is closely reach fom outside of the system. This proves how far Kembang Jepun influences on the morphological and configurational structure of Surabaya as a whole. For this reason, it is no surprise that there is an increasing interest of many parties in promoting the street of Kembang Jepun in the economy activity of the city. Abstract in Bahasa Indonesia : Analisa yang berbasis teknologi informasi dengan menggunakan teknik analisa space syntax menunjukan bahwa dari waktu ke waktu jalan Kembang Jepun merupakan jalan dominan kota Surabaya. Diskripsi dilakukan melalui penggambaran kembali peta Surabaya 2002 dalam tampilan peta integrasi global (Rn=radius infinity) dan peta integrasi lokal (R3=radius '3'). Hasil pengukuran menunjukan bahwa jalan Kembang Jepun secara global berada di dalam kerangka-garis-utama kota (urban super grid); secara lokal jalan ini tidak hanya mempunyai nilai integrasi (integration) tertinggi diantara jalan yang lain, tetapi juga mempunyai nilai keterkaitan (connectivity) dengan jalan yang lain terbanyak, serta mempunyai kedalaman (depth) yang kurang dari luar sistim kota. Adanya kenyataan ini menunjukan betapa kuatnya pengaruh jalan Kembang Jepun dalam struktur morfologi Surabaya dan konfigurasi jalan secara keseluruhan. Maka tidaklah heran bahwa jalan ini mengundang minat berbagai pihak - pemerintah daerah maupun swasta - untuk lebih meningkatkan fungsinya dalam kegiatan ekonomi kota. Kata kunci: teknik analisa space syntax, konfigurasi, jalan dominan.
THE LOGIC OF SPACE IN THE TRANSFORMATION OF POHE SETTLEMENT PATTERN OF GORONTALO CITY, INDONESIA
BAMBANG DJAU,ENDANG TITI SUNARTI DARJOSANJOTO
Journal of Applied Technology in Environmental Sanitation , 2011,
Abstract: Settlement can be defined as a place or an area in which people are concentrated and live together utilizing local environment as intended for sustaining, performing, and developing their life. The pattern of settlement formation and development usually represents its inhabitant’s expression. For example, the seashore settlement in City of Gorontalo previously has a typical characteristic which is a reflection of community activities that are based on waterfront orientation. Nevertheless, if it is analyzed using the term of the logic of space, this pattern of settlement has been transforming within three periods of government change. The building that was originally composed longitudinal following the coastline, has been changed into the composition groups of buildings that make up the road. The existence of this fact shows that the Pohe’s settlement is a phenotype settlement which has experienced the formative process that infiltrating.
Land Characteristics of Batang Pelepat Watershed in Bungo District, Jambi
Sunarti
Jurnal Tanah Tropika , 2010,
Abstract: Land characteristics describebiophysics characteristics of watershed. But, land has been used for economic oriented. The objective of this researchis to identify land characteristics of Batang Pelepat watershed. Data collection was carried out by survey based on landunit map and analyzed by descriptive analysis. The results showed that land in Batang Pelepat watershed consist of 23land units and some land use types (forest, rubber and oil palm farming, settlement and shrub), soil parent materialsvariously (alluvium, granite, tuff andesite, basalt, and clay rock), soil depth ranges from 88 to 160 cm and soil texture isclassified moderate fine to fine. Lands were dominated by slope of >15–30% and >45–65% and dystrudepts of soilgroup with soil fertility level very low to low because its pH about 3.80-6.20, base saturation about 7.86-32.79% and Pavailableabout 2.80-25.00 ppm. Various land use has also caused different erosion and permeability levels
Coupling Analysis of Electromagnetic Field Transverse Magnetic (TM) on 2D Photonic Crystals with Symmetrical Refractive Index Defect Using Tensor Green Method
Candra Kurniawan
Physics , 2011,
Abstract: Photonic crystal is a dielectric material which has a refractive index or permittivity which vary periodically, thus preventing the propagation of light with specific frequency and direction. The frequency range is called a photonic bandgap (PBG). If the structure of photonic crystals is modified by taking one line of rod in a photonic crystal is obtained a waveguide. Giving structures with symmetrical defects around the waveguide channel with the parameters given occurs coupling between the waveguide and cavity. Coupling indicates transfer part or all of the EM field depends on the frequency of EM field in the photonic crystal. Green tensor method can be used to calculate the total electric field in a 2D photonic crystal. With the aid of MATLAB programs can be shown to the powerful visualization calculation of the total electrical field in 2D photonic crystals. Based on analysis of the literature and the bandgap graphic and the field energy in the defects show that effective frequency which produces maximum energy when coupling occur was in the mid-bandgap frequency interval.
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