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Search Results: 1 - 10 of 75338 matches for " Szu-Wei Lee "
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Regulation of Skp2 Expression and Activity and Its Role in Cancer Progression
Chia-Hsin Chan,Szu-Wei Lee,Jing Wang,Hui-Kuan Lin
The Scientific World Journal , 2010, DOI: 10.1100/tsw.2010.89
Exogenous interleukin-6, interleukin-13, and interferon-gamma provoke pulmonary abnormality with mild edema in enterovirus 71-infected mice
Szu-Wei Huang, Yi-Ping Lee, Yu-Ting Hung, Chun-Hung Lin, Jih-Ing Chuang, Huan-Yao Lei, Ih-Jen Su, Chun-Keung Yu
Respiratory Research , 2011, DOI: 10.1186/1465-9921-12-147
Abstract: To test whether EV71-induced PE required a proinflammatory cytokine response, exogenous pro-inflammatory cytokines were administered to EV71-infected mice during the late stage of infection.After intracranial infection of EV71/MP4, 7-day-old mice developed hind-limb paralysis, pulmonary dysfunction, and emphysema. A transient increase was observed in serum IL-6, IL-10, IL-13, and IFN-γ, but not noradrenaline. At day 3 post infection, treatment with IL-6, IL-13, and IFN-γ provoked mild PE and severe emphysema that were accompanied by pulmonary dysfunction in EV71-infected, but not herpes simplex virus-1 (HSV-1)-infected control mice. Adult mice did not develop PE after an intracerebral microinjection of EV71 into the nucleus tractus solitarii (NTS). While viral antigen accumulated in the ventral medulla and the NTS of intracerebrally injected mice, neuronal loss was observed in the ventral medulla only.Exogenous IL-6, IL-13, and IFN-γ treatment could induce mild PE and exacerbate pulmonary abnormality of EV71-infected mice. However, other factors such as over-activation of the sympathetic nervous system may also be required for the development of classic PE symptoms.Enterovirus 71 (EV71), a highly neurotropic, positive-sense single-stranded RNA virus, belongs to the Enterovirus genus of Picornaviridae family. In general, EV71 infections are mild, such as hand, foot, and mouth disease and herpangina in young children. However, central nervous system (CNS) infections with life-threatening pulmonary and cardiac complications have occurred [1]. EV71 has been regarded as the most important neurotropic enterovirus since the effective control of the poliovirus (PV). More than a dozen severe EV71 outbreaks have been reported worldwide since it was first recognized in California in 1969 [2].Pulmonary edema (PE) and subsequent rapid onset cardiopulmonary failure are hallmarks of EV71 induced mortality [3]. EV71-induced PE has been considered neurogenic in origin, as it has bee
Molecular Epidemiology of Enterovirus 71 Infection in the Central Region of Taiwan from 2002 to 2012
Wen-Hao Wu, Ta-Cheng Kuo, Yu-Ting Lin, Szu-Wei Huang, Hsin-Fu Liu, John Wang, Yi-Ming Arthur Chen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0083711
Abstract: Enterovirus 71 (EV71), a causative agent of hand, foot, and mouth disease can be classified into three genotypes and many subtypes. The objectives of this study were to conduct a molecular epidemiological study of EV71 in the central region of Taiwan from 2002–2012 and to test the hypothesis that whether the alternative appearance of different EV71 subtypes in Taiwan is due to transmission from neighboring countries or from re-emergence of pre-existing local strains. We selected 174 EV71 isolates and used reverse transcription-polymerase chain reaction to amplify their VP1 region for DNA sequencing. Phylogenetic analyses were conducted using Neighbor-Joining, Maximum Likelihood and Bayesian methods. We found that the major subtypes of EV71 in Taiwan were B4 for 2002 epidemic, C4 for 2004–2005 epidemic, B5 for 2008–2009 epidemic, C4 for 2010 epidemic and B5 for 2011–2012 epidemic. Phylogenetic analysis demonstrated that the 2002 and 2008 epidemics were associated with EV71 from Malaysia and Singapore; while both 2010 and 2011–2012 epidemics originated from different regions of mainland China including Shanghai, Henan, Xiamen and Gong-Dong. Furthermore, minor strains have been identified in each epidemic and some of them were correlated with the subsequent outbreaks. Therefore, the EV71 infection in Taiwan may originate from pre-existing minor strains or from other regions in Asia including mainland China. In addition, 101 EV71 isolates were selected for the detection of new recombinant strains using the nucleotide sequences spanning the VP1-2A-2B region. No new recombinant strain was found. Analysis of clinical manifestations showed that patients infected with C4 had significantly higher rates of pharyngeal vesicles or ulcers than patients infected with B5. This is the first study demonstrating that different EV 71 genotypes may have different clinical manifestations and the association of EV71 infections between Taiwan and mainland China.
Risk Factors for HIV-1 seroconversion among Taiwanese men visiting gay saunas who have sex with men
Yen-Ju Chen, Yu-Ting Lin, Marcelo Chen, Szu-Wei Huang, Su-Fen Lai, Wing-Wai Wong, Hung-Chin Tsai, Yu-Huei Lin, Hsin-Fu Liu, Shu-Yu Lyu, Yi-Ming A Chen
BMC Infectious Diseases , 2011, DOI: 10.1186/1471-2334-11-334
Abstract: Patrons of 5 gay saunas were recruited for a weekly volunteer counseling and testing program from 2001 to 2005. Questionnaires were collected for a risk factor analysis. HIV-1 subtypes were determined using DNA sequencing and phylogenetic analyses.HIV-1 prevalence rates among MSM in gay saunas in 2001 through 2005 were 3.4%, 5.1%, 8.9%, 8.5%, and 8.3%, respectively. In total, 81 of 1, 093 (7.4%) MSM had HIV-1 infection. Fifty-two HIV-1 strains were genotyped, and all of them were subtype B. HIV-seropositive men were significantly younger than the seronegatives. Only 37.1% used condoms every time during sexual intercourse. A multivariate logistic regression analysis showed that the risk factors for HIV-1 were being uncircumcised (odds ratio (OR) = 2.19; 95% confidence interval (CI), 1.08~4.45); having sexual intercourse with at least 2 partners during each sauna visit (≥ 2 vs. ≤ 1, OR = 1.71; 95% CI, 1.02~2.89); and the role played during anal intercourse (versatile vs. an exclusively insertive role, OR = 2.76; 95% CI, 1.42~5.36).Overall, 7.4% Taiwanese MSM participating in this study had HIV-1 subtype B infection. Uncircumcised, being versatile role during anal intercourse, and having sex with more than one person during each sauna visit were main risk factors for HIV-1 infection.Since the global outbreak of HIV/AIDS, HIV has been transmitted among different vulnerable populations. According to a recent review, the global trend of HIV-1 infection among men having sex with men (MSM) has continued to increase, especially in East Asia, Africa, and Russia [1]. As of September 2011, there were 22, 335 known cases of HIV infection in Taiwan, and Taiwanese nationals accounted for 96.5% of those cases. Of the Taiwanese nationals, 92.5% were male. Populations at risk for HIV infection include injection drug users (30.4%), homosexual men (38.3%), heterosexual men (21.6%), and bisexual men (8.1%). Therefore, MSM are a high risk group for HIV infection in Taiwan [2].Since 1996~
Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest
Jing-Iong Yang,Chi-Chen Yeh,Jin-Ching Lee,Szu-Cheng Yi,Hurng-Wern Huang,Chao-Neng Tseng,Hsueh-Wei Chang
Molecules , 2012, DOI: 10.3390/molecules17067241
Abstract: Potential antioxidant properties of an aqueous extract of the edible red seaweed Gracilaria tenuistipitata (AEGT) against oxidative DNA damage were evaluated. The AEGT revealed several antioxidant molecules, including phenolics, flavonoids and ascorbic acid. In a cell-free assay, the extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity that significantly reduced H2O2-induced plasmid DNA breaks in a dose-response manner (P < 0.001). The AEGT also suppressed H2O2-induced oxidative DNA damage in H1299 cells by reducing the percentage of damaged DNA in a dose-response manner (P < 0.001) as measured by a modified alkaline comet-nuclear extract (comet-NE) assay. The MTT assay results showed that AEGT confers significant protection against H2O2-induced cytotoxicity and that AEGT itself is not cytotoxic (P < 0.001). Moreover, H2O2-induced cell cycle G2/M arrest was significantly released when cells were co-treated with different concentrations of AEGT (P < 0.001). Taken together, these findings suggest that edible red algae Gracilaria water extract can prevent H2O2-induced oxidative DNA damage and its related cellular responses.
Metabolic classification of microbial genomes using functional probes
Chi-Ching Lee, Wei-Cheng Lo, Szu-Ming Lai, Yi-Ping Chen, Chuan Tang, Ping-Chiang Lyu
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-157
Abstract: We have developed a proteome-based method for classifying microbial species. This classification method uses a set of probes comprising short, highly conserved amino acid sequences. For each genome, in silico translation is performed to obtained its proteome, based on which a probe-set frequency pattern is generated. Then, the probe-set frequency patterns are used to cluster the proteomes/genomes.Features of the proposed method include a high running speed in challenge of a large number of genomes, and high applicability for classifying organisms with incomplete genome sequences. Moreover, the probe-set clustering method is sensitive to the metabolic phenotypic similarities/differences among species and is thus supposed potential for the classification or differentiation of closely-related organisms.Owing to new sequencing technologies, the number of microorganisms with completely or partially determined genomic sequences is rapidly increasing, inclusive of many species that cannot be artificially cultured and many new/unknown species collected from environmental samples. As the amount of genomic information increases, interdependent relationships between species (e.g., the symbiotic partnership between bacteria and host) and the survival strategy of certain microbes and their living environment (e.g., Archaea and Bacteria living in the hot spring) become particularly interesting. As a result, the microbiology field has gradually expanded its focus from microbial clones to microbial communities, and some new research fields have accordingly formed, such as metagenomics [1]. Because species are no longer studied through clonal isolates, the first question encountered by microbial genomics researchers looking at a heterogeneous population is often "Who is there?". A number of short biomarkers, such as the 16S rRNA genes, exhibit detectable sequence variations in a basically conserved framework between species and can be used both to identify individual species within
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells
Szu-Hsiu Liu, Lain-Tze Lee, Nai-Yun Hu, Kuo-Kuei Huange, Ying-Chu Shih, Iinuma Munekazu, Jen-Ming Li, Ting-Yu Chou, Wei-Hsin Wang, Ting-Shou Chen
Chinese Medicine , 2012, DOI: 10.1186/1749-8546-7-19
Abstract: U937 and EL4 cells were treated with different concentrations of α-MG in the presence of 0.1?ng/mL lipopolysaccharide (LPS) for 4?h. The anti-inflammatory effects of α-MG were measured by the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-4 in cell culture media, which were determined with enzyme-linked immunosorbent assay kits. The gene expression profiles of all samples were analyzed with a whole human genome microarray, Illumina BeadChip WG-6 version 3, containing 48804 probes. The protein levels were determined by Western blotting analyses.α-MG decreased the LPS induction of the inflammatory cytokines TNF-α (P?=?0.038) and IL-4 (P?=?0.04). α-MG decreased the gene expressions in oncostatin M signaling via mitogen-activated protein kinase (MAPK) pathways, including extracellular signal-regulated kinases (P?=?0.016), c-Jun N-terminal kinase (P?=?0.01) , and p38 (P?=?0.008). α-MG treatment of U937 cells reduced the phosphorylation of MAPK kinase 3 / MAPK kinase 6 (P?=?0.0441), MAPK-activated protein kinase-2 (P?=?0.0453), signal transducers and activators of transcription-1 (STAT1) (P?=?0.0012), c-Fos (P?=?0.04), c-Jun (P?=?0.019) and Ets-like molecule 1 (Elk-1) (P?=?0.038).This study demonstrates that α-MG attenuates LPS-mediated activation of MAPK, STAT1, c-Fos, c-Jun and EIK-1, inhibiting TNF-α and IL-4 production in U937 cells.The mangosteen fruit has been used in Chinese and Ayurvedic medicine [1]. Extracts of mangosteen have antioxidant, antitumor, anti-inflammatory, antiallergic, antibacterial, antifungal and antiviral effects [1-3]. α-Mangostin (α-MG), which was first isolated from the mangosteen in 1855, is a competitive antagonist of the histamine H1 receptor and possesses many biological properties, such as anti-inflammatory, anti-oxidative damage and antioxidant activities [4-6]. Previous studies have shown that α-MG significantly inhibits nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α and inducible NOS (iNOS) pro
Cognitive Control in Opioid Dependence and Methadone Maintenance Treatment
Ding-Lieh Liao, Cheng-Yi Huang, Sien Hu, Su-Chen Fang, Chi-Shin Wu, Wei-Ti Chen, Tony Szu-Hsien Lee, Pau-Chung Chen, Chiang-shan R. Li
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094589
Abstract: Background Substance misuse is associated with cognitive dysfunction. We used a stop signal task to examine deficits in cognitive control in individuals with opioid dependence (OD). We examined how response inhibition and post-error slowing are compromised and whether methadone maintenance treatment (MMT), abstinence duration, and psychiatric comorbidity are related to these measures in individuals with OD. Methods Two-hundred-and-sixty-four men with OD who were incarcerated at a detention center and abstinent for up to 2 months (n = 108) or at a correctional facility and abstinent for approximately 6 months (n = 156), 65 OD men under MMT at a psychiatric clinic, and 64 age and education matched healthy control (HC) participants were assessed. We computed the stop signal reaction time (SSRT) to index the capacity of response inhibition and post-error slowing (PES) to represent error-related behavioral adjustment, as in our previous work. We examined group effects with analyses of variance and covariance analyses, followed by planned comparisons. Specifically, we compared OD and HC participants to examine the effects of opioid dependence and MMT and compared OD sub-groups to examine the effects of abstinence duration and psychiatric comorbidity. Results The SSRT was significantly prolonged in OD but not MMT individuals, as compared to HC. The extent of post-error slowing diminished in OD and MMT, as compared to HC (trend; p = 0.061), and there was no difference between the OD and MMT groups. Individuals in longer abstinence were no less impaired in these measures. Furthermore, these results remained when psychiatric comorbidities including misuse of other substances were accounted for. Conclusions Methadone treatment appears to be associated with relatively intact cognitive control in opioid dependent individuals. MMT may facilitate public health by augmenting cognitive control and thereby mitigating risky behaviors in heroin addicts.
P Wave Dispersion and Maximum P Wave Duration Are Independently Associated with Rapid Renal Function Decline
Ho-Ming Su, Wei-Chung Tsai, Tsung-Hsien Lin, Po-Chao Hsu, Wen-Hsien Lee, Ming-Yen Lin, Szu-Chia Chen, Chee-Siong Lee, Wen-Chol Voon, Wen-Ter Lai, Sheng-Hsiung Sheu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042815
Abstract: The P wave parameters measured by 12-lead electrocardiogram (ECG) are commonly used as noninvasive tools to assess for left atrial enlargement. There are limited studies to evaluate whether P wave parameters are independently associated with decline in renal function. Accordingly, the aim of this study is to assess whether P wave parameters are independently associated with progression to renal end point of ≥25% decline in estimated glomerular filtration rate (eGFR). This longitudinal study included 166 patients. The renal end point was defined as ≥25% decline in eGFR. We measured two ECG P wave parameters corrected by heart rate, i.e. corrected P wave dispersion (PWdisperC) and corrected P wave maximum duration (PWdurMaxC). Heart function and structure were measured from echocardiography. Clinical data, P wave parameters, and echocardiographic measurements were compared and analyzed. Forty-three patients (25.9%) reached renal end point. Kaplan-Meier curves for renal end point-free survival showed PWdisperC > median (63.0 ms) (log-rank P = 0.004) and PWdurMaxC > median (117.9 ms) (log-rank P<0.001) were associated with progression to renal end point. Multivariate forward Cox-regression analysis identified increased PWdisperC (hazard ratio [HR], 1.024; P = 0.001) and PWdurMaxC (HR, 1.029; P = 0.001) were independently associated with progression to renal end point. Our results demonstrate that increased PWdisperC and PWdurMaxC were independently associated with progression to renal end point. Screening patients by means of PWdisperC and PWdurMaxC on 12 lead ECG may help identify a high risk group of rapid renal function decline.
High Incidence of Ischemic Stroke Occurrence in Irradiated Lung Cancer Patients: A Population-Based Surgical Cohort Study
Shih-Kai Hung, Moon-Sing Lee, Wen-Yen Chiou, Ching-Chih Lee, Yi-Chun Chen, Chun-Liang Lai, Nai-Chuan Chien, Wen-Lin Hsu, Dai-Wei Liu, Yu-Chieh Su, Szu-Chi Li, Hung-Chih Lai, Shiang-Jiun Tsai, Feng-Chun Hsu, Hon-Yi Lin
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094377
Abstract: Background and Purpose A high risk of stroke occurrence has been reported in several types of irradiated cancer patients. However, clinical data are lacking in irradiated lung cancer patients. The present study intended to explore a risk level of ischemic stroke occurrence in irradiated lung cancer patients. Methods A nationwide population-based database obtained from the Taiwan National Health Insurance was analyzed. Between 2003 and 2006, we recruited 560 resected lung cancer patients into two study groups: surgery-plus-irradiation (n = 112) and surgery-alone (n = 448). Patients treated with chemotherapy were excluded. Propensity score match was used for pairing cases with a ratio of 1:4. Two-year ischemic-stroke-free survival was defined as the primary endpoint. Results Three observations supported a high risk of ischemic stroke occurrence in patients with postoperative irradiation when compared with those patients with surgery alone: first, a high incidence per 1,000 person-year (22.3 versus 11.2, 1.99 folds); second, a low two-year ischemic-stroke-free survival rate (92.2% versus 98.1%, P = 0.019); and third, a high adjusted hazard ratio (HR, 4.19; 95% CI, 1.44–12.22; P = 0.009). More notably, the highest risk of ischemic stroke occurrence was found in irradiated patients who had diabetes mellitus (HR, 34.74; 95% CI, 6.35->100; P<0.0001). Conclusions A high incidence of ischemic stroke was observed in irradiated lung cancer patients, especially in those with diabetes mellitus. For these patients, close clinical surveillance and strict diabetes control should be considered. Further studies to define detail biological mechanisms are encouraged.
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