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Search Results: 1 - 10 of 4406 matches for " Svetlana Milija?evi? "
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Clavibacter michiganensis subsp. michiganensis, Bacterial Canker of Tomato: 1. Conventional and Molecular Identification
Svetlana Milijaevi,Biljana Todorovi?,Jelica Bala?
Pesticidi i Fitomedicina , 2006,
Abstract: In May 2006, tomato leaves with watersoaked areas between leaf veins were detected in glasshouses in Padinska Skela. The early-stage symptoms were followed by rapid desiccation to white and pale brown necrosis and subsequently by wilting of entire tomato plants. In June 2006, symptoms of bacterial canker and wilt were also recorded in manygreenhouses in the Jablanica County in southern Serbia.Samples of diseased tomato plants were collected from several tomato-growing regions in Serbia in order to identify the causal agent using conventional identification methods (isolation, pathogenicity and bacteriological determinative tests). Another objectiveof this study was to confirm the identity of isolated bacterial strains by polymerase chain reaction (PCR method).According to the morphological characteristics observed on NA and NBY media, biochemical characteristics, hypersensitive response in four-o’clock plant leaves and pathogenicity test on tomato seedlings, the investigated strains were identified as C. michiganensis subsp. michiganensis. To confirm the identity of isolated strains two PCR protocols were used. Amplification of expected length DNA fragments, 614 bp and 270 bp, respectively, confirmed that the investigated strains belonged to C. michiganensis subsp. michiganensis.
First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia
Svetlana Milijaevi,Veljko Gavrilovi?,Svetlana ?ivkovi?,Nenad Trkulja
Pesticidi i Fitomedicina , 2007,
Abstract: During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemicaland physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 genelocated on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia.
Erwinia amylovora – the Causal Agent of Root Collar Necrosis of Apple Trees
Veljko Gavrilovi?,Svetlana Milijaevi,Biljana Todorovi?,Svetlana ?ivkovi?
Pesticidi i Fitomedicina , 2008,
Abstract: A large-scale outbreak of fire blight symptoms caused by Erwinia amylovora was recorded in pome fruit trees during 2007. In addition to fruit necrosis and shoot blight as the typical disease symptoms, dark purple necrosis was observed in the root collar area girdling the trunk just above the ground and thus withering the whole apple tree. Since similar symptoms on apple trees could be caused by E. amylovora or one of several phytopathogenic fungi of the genera Phomopsis and Phytophthora, an investigation was conducted to identify the causal agent of this disease. Levan-producing, nonfluorescent bacteria were isolated from diseased samples. The isolated strains produced HR in tobacco leaves and necrosis of artificially inoculated, immature pear fruits, followed by oozing of bacterial exudate, a characterisitic of E. amylovora. Based on the results of pathogenicity tests, biochemical characteristics, ELISA test and PCR analysis, it was confirmed that the investigated strainsbelonged to E. amylovora, causing the root collar necrosis of apple trees as an atypical symptom of this bacterium in Serbia. The explanation of this symptom may be that the vegetative rootstocks were infected with E. amylovora. Therefore, the development of diagnostic protocols for detection of E. amylovora in apple rootstock is very important for health inspections of planting materials.
Sensitivity of Cladobotryum spp., a Pathogen of the Button Mushroom (Agaricus bisporus), to Some Fungicides
Ivana Poto?nik,Svetlana Milijaevi,Emil Rekanovi?,Biljana Todorovi?
Pesticidi i Fitomedicina , 2007,
Abstract: Thirteen isolates of Cladobotryum spp., isolated from diseased fruiting bodies of Agaricus bisporus, sampled from mushroom farms in Serbia during 2003-2006, were studied. Sensitivity to several selected fungicides was tested and all isolates were found to be highly sensitive to iprodione (EC50 = 0.35-2.30 mg L-1), benomyl (EC50 = 0.14-0.97 mg L-1) and especially to prochloraz-Mn (EC50 = 0.02-0.09 mg L-1). The minimum inhibitory concentration (MIC) of benomyl was 4 mg L-1, while those of prochloraz-Mn and iprodione exceeded 1000 mg L-1. The minimum fungicide concentration (MFC) of benomyl was 4 mg L-1, and none of the tested concentrations of prochloraz-Mn and iprodione were lethal to any of the investigated Cladobotryum spp. isolates.
Clavibacter michiganensis subsp. michiganensis, Bacterial Canker of Tomato: 2. Comparison of the Effectiveness of Extraction Procedures and Sensitivity of Methods for Detection in Tomato Seeds
Svetlana Milijaevi,Biljana Todorovi?,Emil Rekanovi?,Ivana Poto?nik
Pesticidi i Fitomedicina , 2007,
Abstract: Two seed extraction procedures, used for detection of Clavibacter michiganensis subsp. michiganensis (Cmm) in artifficially infested tomato seed lots, were evaluated. A comparison of the efficiency of pathogen detection by using different extraction methods showed that a grinding procedure was more effective than soaking seed samples. The extraction by grinding resulted in a higher number of samples with Cmm colonies than did the method that included soaking. The detection threshold of Cmm in relation to seed sample size wasevaluated by adding different numbers of artificially infested seeds to uninfected samples of 2000 or 5000 seeds. Four detection methods were simultaneously compared for their sensitivity in Cmm detection in seeds: isolation on semiselective media (mSCM, D2ANX, mCNS), direct PCR from seed material, Bio-PCR with initial culturing of bacteria on NBY agar prior to PCR, and Enrichment PCR. The pathogen was detected in samples of 2000 seeds containing one, five and ten infested seeds, in at least two out of three replicates by threedetection methods (selective plating, direct PCR and Bio-PCR), using the grinding extraction method with an addition of centrifugation step. In samples of 5000 seeds, five infested seeds were detected in all replicates by the same detection methods. Similar resultswere obtained by the soaking extraction method. In Enrichment PCR, positive results were obtained only in samples of 2000 seeds containing five and ten infested seeds regardless of the extraction method.
In vitro Toxicity of Fungicides of Different Mode of Action to Agaricus bisporus (Lange) Imbach
Ivana Poto?nik,Emil Rekanovi?,Svetlana Milijaevi,Biljana Todorovi?
Pesticidi i Fitomedicina , 2009,
Abstract: Isolates of Agaricus bisporus strains F56 and U3 were tested for sensitivity to several selected fungicides in vitro. The analysis showed that flusilasole + carbendazim and cyproconazole + carbendazim were the most toxic fungicides to A. bisporus strain F56 with respective EC50 values of 0.04 and 0.23 mg/l. The least toxic fungicides were carbendazim (EC50 = 16.58 mg/l) and trifloxystrobin (EC50 = 20.69 mg/l) to A. bisporus F56 and benomyl (EC50 = 14.99 mg/l) to A. bisporus U3.
Field Efficacy of Fluopicolide and Fosetyl-Al Fungicide Combination (Profiler ) for Control of Plasmopara viticola (Berk. & Curt.) Berl. & Toni. in Grapevine
Emil Rekanovi?,Ivana Poto?nik,Milo? Stepanovi?,Svetlana Milijaevi
Pesticidi i Fitomedicina , 2008,
Abstract: The efficacy of new fungicide mixtures in controlling Plasmopara viticola in grapevine was evaluated in field trials. The efficacies of Profiler (fluopicolide + fosetyl-Al) and the standard fungicide Mikal Flash (fosetyl-Al + folpet) were tested at Radmilovac and Slankamena ki Vinogradi in 2006 and 2007. Both tested fungicides exhibited high efficacy in controlling grape downy mildew. There were no significant differencies in the efficacies of Profiler(96.1-99.7%) and Mikal Flash (94.9-99.2%). Our experiments showed that the investigated fungicide mixture fluopicolide + fosetyl-Al is highly effective against P. viticola, even when it it is applied at long intervals and under high disease pressure.
Effects of Copper-based Compounds, Antibiotics and a Plant Activator on Population Sizes and Spread of Clavibacter michiganensis subsp. michiganensis in Greenhouse Tomato Seedlings
Svetlana Milijaevi,Biljana Todorovi?,Ivana Poto?nik,Emil Rekanovi?
Pesticidi i Fitomedicina , 2009,
Abstract: Three copper-based compounds (copper hydroxide, copper oxychloride, copper sulphate), two antibiotics (streptomycin and kasugamycin) and a plant activator (ASM) significantly reduced population sizes and spread of C. michiganensis subsp. michiganensis among tomatoseedlings in the greenhouse. Streptomycin had the best effect in reducing pathogen population size in all sampling regions. Moreover, this antibiotic completely stopped the spread of C. michiganensis subsp. michiganensis in the region most distant from the inoculumfocus. Copper hydroxide mixed with streptomycin significantly limited the pathogen population, compared with copper hydroxide alone, the other copper-based compounds, ASM and kasugamycin. However, combining streptomycin with copper hydroxide did notcontribute to its greater efficacy against the pathogen population. Copper-based compounds, in general, were less effective in limiting pathogen population sizes than the other treatments in all three sampling regions, primarily copper oxychloride and the combinationof copper hydroxide and mancozeb. Among copper compounds, copper hydroxide was the most prominent in reducing the bacterial population, especially in the region closest to the inoculum focus, while its combination with mancozeb did not improve the effects. Kasugamycin significantly limited pathogen population size, compared to copper bactericides, but it was less effective than the other antibiotic compound, i.e. streptomycin. The plant activator ASM significantly reduced population density, and it was more effectivewhen used three days prior to inoculation than six days before inoculation.
Susceptibility of Bean Genotypes to Xanthomonas campestris pv. phaseoli in Greenhouse Conditions
Biljana Todorovi?,Svetlana Milijaevi,Emil Rekanovi?,Ivana Poto?nik
Pesticidi i Fitomedicina , 2008,
Abstract: Plants of 17 bean genotypes were evaluated under greenhouse conditions for their reaction to X. campestris pv. phaseoli, the causal agent of common blight of beans, following leaf-spray inoculation with bacterial suspension (108 cfu/ml). The plants were evaluated based on the number of leaf lesions, and the disease severity index (DSI) was calculated.The evaluated genotypes showed various levels of susceptibility to X. campestris pv. phaseoli strain VS-1. The results of both experiments showed that the cultivar Oreol was the most resistant. The genotypes: KB 142, HR-45, Tisa and Panonski tetovac also showed low susceptibility with DSI values in the first trial ranging from 2.27 to 3.60. The same genotypes, with the exception of Panonski tetovac, were also categorized as low-susceptible to the bacterium in the second experiment, having the DSI values between 2.27 and 3.60. Most genotypes (Slavonski utozeleni, Zlatko, Biser, Sremac, Naya Nayahit) were categorized as susceptible in the first experiment, including Panonski tetovac in the second one, while the genotypes Dvadesetica, Prelom and Oplenac displayed the highest susceptibility in both trials.
Control of Xanthomonas campestris pv. phaseoli on Bean_Using Copper Compounds and Plant Activator
Biljana Todorovi?,Svetlana Milijaevi,Emil Rekanovi?,Jelica Bala?
Pesticidi i Fitomedicina , 2006,
Abstract: The efficacy of several new formulations of copper compounds, namely Cuprozin 35 WP (copper-oxychloride), Cuproxat (copper-sulphate), Funguran OH (copper-hydroxide)and the plant activator Bion(acibenzolar-S-methyl), and their combinations with dithiocarbamates(Dithane M-70) was estimated in controlling Xanthomonas campestris pv. phaseoli (artificial inoculation) in field conditions in two localities during 2006. In the locality Zemun, the efficacy of copper compounds ranged from 92.7% to 98.5%. The plant activator Bion 50 WG exhibited similar efficacy (94.4-97.1%). Combinations of Funguran OH and Dithane M-70, applied at different concentrations, also showed high efficacy (98.3-99.3%), as well as the combinations of Bion 50 WG and the other bactericides (95.5-96.8%). There was no significantdifference between the efficacies achieved by the compounds applied individually and their combinations, except Cuproxat, which exhibited decreased efficacy at lower concentration. In the locality Smederevska Palanka, the efficacy of copper compounds was 95.0-98.2%, while Bion achieved 96.8-97.7% efficacy. Combinations of copper-hydroxide(Funguran OH) and dithiocarbamates (Dithane M-70) also showed high efficacy (98.1-99.4%) but without a significant difference. The efficacy of combinations of Bion and copper-hydroxide, and Bion and mancozeb was 97.9-98.9%. There was no significant difference in the efficacies of the bactericides tested or the efficacies of their combinations in that locality.Our investigation confirmed high efficacy of acibenzolar-S-methyl, which was equal to the efficacy of standard bactericide treatment. This compound therefore offers a very good alternative to conventional chemicals used for controlling bacterial diseases in beans.
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