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Search Results: 1 - 10 of 1937 matches for " Svetlana ?ivkovi? "
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Effect of actual vapor pressure on estimating evapotranspiration at Serbia
Trajkovi? Slavi?a,ivkoviSvetlana
Facta Universitatis Series : Architecture and Civil Engineering , 2009, DOI: 10.2298/fuace0902171t
Abstract: Actual vapor pressure (VP) is an important parameter that is used in many evapotranspiration equations. However, vapor pressure is difficult to measure accurately. In the humid climate, the actual vapor pressure can be derived from minimum air temperature. The objectives of this study were: first, to estimate errors that can arise if VP data are not available and have to be estimated; second, to compare the Priestley-Taylor ET0 values computed under various levels of VP data availability; and third, to evaluate the reliability of Priestley-Taylor equation as compared to the FAO-56 Penman-Monteith method. The following main conclusions can be drawn: Estimated VP values generally were in closest agreement with measured VP values. The measurements of air humidity at humid locations are not indispensable for estimating reference evapotranspiration. The Priestley-Taylor method (with measured or estimated VP) provides the very good agreement with the evapotranspiration obtained by the FAO-56 Penman-Monteith method except windless locations.
Multiple gigantic renal cysts
ivkovi? Sla?ana,Pavlovi? Svetlana,?iri? Slobodan
Vojnosanitetski Pregled , 2009, DOI: 10.2298/vsp0902163z
Abstract: Introduction. Cystic renal lesions are very heterogeneous lesions which differ in ethiopathogenesis, morphological and clinical manifestations, and also in evolution and therapy. Classification of cystic lesions is complex, symptomatology is poor, and diagnosis is based on complete radiological diagnostic procedures. Case report. We presented a 20-year old patient with mild subjective symptoms. Objectively, he was without positive clinical signs and changes in biochemistry of blood. Using ultrasonography (US) multiple serous simple cysts were found in both kidneys. Using computed tomography (CT) multiple serous cysts were found, without changes in cystic walls, with preserved renal parenchyma and without cystic changes on other parenchymatous organs. Conclusion. Although renal cystic lesions are frequent in adult population, this is a rare example of a young adult man with simple, gigantic, serous cysts which do not produce clinical manifestations nor functional renal difficulty so far.
Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides
ivkoviSvetlana,Stojanovi? S.,Ivanovi? ?.,Gavrilovi? V.
Archives of Biological Sciences , 2010, DOI: 10.2298/abs1003611z
Abstract: The antagonistic activities of five biocontrol agents: Trichoderma harzianum, Gliocladium roseum, Bacillus subtilis, Streptomyces noursei and Streptomyces natalensis, were tested in vitro against Colletotrichum acutatum and Colletotrichum gloeosporioides, the causal agents of anthracnose disease in fruit crops. The microbial antagonists inhibited mycelial growth in the dual culture assay and conidial germination of Colletotrichum isolates. The two Streptomyces species exhibited the strongest antagonism against isolates of C. acutatum and C. gloeosporioides. Microscopic examination showed that the most common mode of action was antibiosis. The results of this study identify T. harzianum, G. roseum, B. subtilis, S. natalensis and S. noursei as promising biological control agents for further testing against anthracnose disease in fruits. .
First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia
Svetlana Milija?evi?,Veljko Gavrilovi?,Svetlanaivkovi,Nenad Trkulja
Pesticidi i Fitomedicina , 2007,
Abstract: During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemicaland physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 genelocated on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia.
Erwinia amylovora – the Causal Agent of Root Collar Necrosis of Apple Trees
Veljko Gavrilovi?,Svetlana Milija?evi?,Biljana Todorovi?,Svetlanaivkovi
Pesticidi i Fitomedicina , 2008,
Abstract: A large-scale outbreak of fire blight symptoms caused by Erwinia amylovora was recorded in pome fruit trees during 2007. In addition to fruit necrosis and shoot blight as the typical disease symptoms, dark purple necrosis was observed in the root collar area girdling the trunk just above the ground and thus withering the whole apple tree. Since similar symptoms on apple trees could be caused by E. amylovora or one of several phytopathogenic fungi of the genera Phomopsis and Phytophthora, an investigation was conducted to identify the causal agent of this disease. Levan-producing, nonfluorescent bacteria were isolated from diseased samples. The isolated strains produced HR in tobacco leaves and necrosis of artificially inoculated, immature pear fruits, followed by oozing of bacterial exudate, a characterisitic of E. amylovora. Based on the results of pathogenicity tests, biochemical characteristics, ELISA test and PCR analysis, it was confirmed that the investigated strainsbelonged to E. amylovora, causing the root collar necrosis of apple trees as an atypical symptom of this bacterium in Serbia. The explanation of this symptom may be that the vegetative rootstocks were infected with E. amylovora. Therefore, the development of diagnostic protocols for detection of E. amylovora in apple rootstock is very important for health inspections of planting materials.
Characteristics of Bacterial Strains from Pseudomonas Genera Isolated from Diseased Plum Trees
Veljko Gavrilovi?,Svetlanaivkovi,Nenad Trkulja,Mirko Ivanovi?
Pesticidi i Fitomedicina , 2008,
Abstract: Characteristics of Pseudomonas syringae strains isolated from diseased plum trees are presented is this paper. Based on pathogenic, biochemical and physiological characteristics, isolated starins were divided into two groups: First group of strains, isolated from diseased plum branches with symptoms of suden decay, was simillar to Pseudomonas syringae pv. syringae; second group of strains, isolated from necrotic flower buds on plum trees, exhibited characteristics simillar to Pseudomonas syringae pv. morsprunorum. In addition, phytopathogenic fungi belonging to genera Phomopsis, Botryosphaeria and Leucostoma, were also isolated from diseased plum trees. Further study of these pathogens and their role in the epidemiology of suden plum trees decay is in progress.
Pseudomonas syringae – Pathogen of Sweet Cherry in Serbia
Veljko Gavrilovi?,Svetlanaivkovi,Nenad Dolovac,Nenad Trkulja
Pesticidi i Fitomedicina , 2012,
Abstract: Characteristics of pathogenic Pseudomonas bacterial strains isolated from cherry inSerbia are presented in the article. Two types of symptoms were observed on cherry treesat few localities with intensive production in Serbia (Belgrade, a ak, Topola, abac, NoviSad). The first symptom is bud necrosis and the second bacterial canker of cherry branch.Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin ofnecrotic tissue. All investigated strains were levan and HR positive, while negative resultswere recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +).Based on pathogenicity tests and differential GATT tests, investigated strains weredivided in two distinct groups: the first group consisted of strains isolated from necroticcherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits,syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartratenegative (typical characteristics of P.s. pv. syringae). Contrary, second group strainswere isolated from necrotic cherry buds, showed negative results in mentioned pathogenicitytests, gelatin and aesculin tests were negative, while tyrosinase and tartrate werepositive (typical characteristics of P.s. pv. morsprunorum).REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. spv. morsprunorum compared to referent strain. In contrast, isolates obtained from necroticcherry branches had unique fingerprint profiles but different from all reference strains.According to the obtained results it was concluded that both pathovars of P. syringae(syringae and morsprunorum) cause necrosis of cherry trees in Serbia.
Morphological and Molecular Identification of Colletotrichum acutatum from Tomato Fruit
Svetlanaivkovi,Sa?a Stojanovi?,?arko IVanovi?,Nenad Trkulja
Pesticidi i Fitomedicina , 2010,
Abstract: Colletotrichum gloeosporioides, Colletotrichum acutatum, Colletotrichum coccodes, and Colletotrichum dematium are the four main species of Colletotrichum that cause tomato anthracnose. In Serbia, the occurrence of anthracnose on tomato fruit has been recorded during the last several years. Typical fruit symptoms include dark, sunken, and circular lesion with orange conidial masses. Pathogen isolates were obtained from a diseased tomato fruits, on PDA medium forming a white to gray colonies. The cultures developed black acervuli around the center of the colony. Conidia were hyaline, aseptate, and fusiform or rarely cylindrical. Appressoria were smooth, simple, clavate to ovate, and variedfrom light to dark brown. Pathogenicity tests with representative isolates were conducted on symptomless, detached tomato fruits. All tested isolates caused anthracnose lesions on tomato fruit after 7 days of incubation. Koch’s postulates were fulfilled by reisolationfrom inoculated tomato fruits. PCR analysis (using species-specific primer pair, CaInt2/ITS4) of genomic DNA from tomato isolates resulted in an amplification product of 490 bp, specific for C. acutatum, further confirming the identity of the pathogen. Based onmorphological and molecular characteristics, the isolates from tomato fruit were determined as C. acutatum.
Hereditary hamartomatous gastrointestinal polyposis syndrome
Vesna ?ivkovi,Svetlana Pejovi?,Aleksandar Nagorni,Bratislav Petrovi?
Acta Facultatis Medicae Naissensis , 2010,
Abstract: Hamartomas represent localized overgrowth of cells in the parts which are normally associated with polyps, ie. mesenhimal, stromal, endodermal and ectodermal elements. Hamartomatous polyposis syndromes carry a significant risk of developing dysplasia, adenomas, gastrointestinal carcinomas, and pancreatic carcinomas. These syndromes may be classified on the basis of whether they represent hereditary syndromes or whether they occur on a sporadic basis. An overlap has been noticed among some of the syndromes. There have been described eight hereditary, and four non-hereditary hamartomatous poly pos syndromes. Hereditary syndromes include: Hereditary juvenile polyposis syndrome, Cowden syndrome, Bannayan-Ruvalcaba-Riley syndrome, Peutz-Jeghers syndrome, Nevus basal cell syndrome, Hereditary mixed polyposis syndrome, Neurofibromathosis type 1, and Multiple Endocrine Neoplasia type 2B. All of these syndromes are inherited in an autosomal dominant fashion. Non-hereditary syndromes include: Cronkhite-Canada syndrome, hyperplastic polyps, lymphoid polyposis, lymphomatous polyposis. The diagnosis of these syndromes primarily remains a clinical process. Treatment of these patients requires a coordinated multidisciplinary approach which includes gastroenterology, pathology, dermatology, surgery, oncology, and genetics.
TENECTEPLASE AS A NEW MEDICATION IN MANAGING NO-REFLOW
Milan ?ivkovi,Svetlana Apostolovi?,Milan Pavlovi?,Sonja ?alinger Martinovi?
Acta Medica Medianae , 2012,
Abstract: No-reflow has been defined as “inadequate myocardial perfusion through a given segment of coronary circulation without angiographic evidence of a mechanical obstruction”. Important components of the process are thought to include endothelial ischemic injury producing “blebs” of tissue that directly obstruct the microvasculature, leukocyte plugging of capillaries, and the vascular effects of reactive oxygen species. No-reflow can complicate any percutaneous intervention (PCI), though it is more common following acute myocardial infarctions (MI), particularly with prolonged occlusion times. A 59-year-old woman presented to the hospital after two hours of continuous chest pain. Because of acute myocardial infarction of the inferior and lateral wall, she underwent direct stenting to an occlusion in the right coronary artery. Despite successful implantation of stents revascularization failed. In absence of aspiration devices and other pharmacological agent we decide to apply 30 mg (6000 IU) tenecteplase intracoronary. Three min after administration TIMI flow grade improved from TIMI 0 to TIMI 3. Managing no-reflow can be approached in a number of different ways and needs to be tailored to the type of intervention being performed. As confirmed in practice, prevention is better than cure and both mechanical and pharmacological approaches can be employed in high risk cases. In the setting of acute myocardial infarction the most effective preventative measure is the rapid opening of the vessel and as such the development of a robust and efficient primary PCI service is integral to the avoidance of this complication. Managing no-reflow will become increasingly important with the wider development of primary PCI. Within the setting of acute myocardial infarctions with no reflow as primary percutaneous intervention complication, there are potential important future pharmacological regimens that may become established and one of them can be tenecteplase.
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