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Effects of Learning Environmental Education Using the 7E-Learning Cycle with Metacognitive Techniques and the Teacher s Handbook Approaches on Learning Achievement, Integrated Science Process Skills and Critical Thinking of Mathayomsuksa 5 Students with Different Learning Achievement
Sutee Sornsakda,Paitool Suksringarm,Adisak Singseewo
Pakistan Journal of Social Sciences , 2012,
Abstract: This research aimed to investigate and compare the effects of learning environmental education using the 7E-learning cycle with metacognitive techniques and the teacher s handbook approaches on learning achievement, integrated science process skill and critical thinking of 93 Mathayomsuksa 5 (grade 11) students with different learning achievements, They were randomly selected by the cluster random sampling technique in the first semester of an academic year 2008. This students were assigned to an experimental group with 45 students and a control group with 48 students. The instruments for the study included: five plans of learning organization using the 7-E learning cycle with 3 metacognitive techniques: intelligibility, plausibility and wide-applicability for the experimental group and 5 plans of learning organization using the teacher s handbook approach for the control group, each plan for 2 h of learning in each week, the learning achievement test, the test on integrated science process skills and the critical thinking test. The paired t-test and the F-test (Two-way MANCOVA) were employed for testing hypothesis. The major findings revealed that the whole students, the high achievers and the low achievers indicated gains in learning achievement, integrated science process skills in general and in 3-5 subscales and critical thinking in general and in each subscale from before learning (p<0.05). The experimental group evidenced more learning achievement, integrated science process skills in general and in each subscale and critical thinking in general and in the interpretation subscale than the control group (p<0.05). The high achievers showed more learning achievement, integrated science process skills in general and in 4 subscales and critical thinking in general and in 4 subscales than the low achievers (p<0.05). Statistical interactions of learning model with learning achievement were found to be significant (p<0.05) in learning achievement, integrated science process skills in general and in 4 subscales and critical thinking in general and in 3 subscales.
Chetsada Noknoi,Sutee Ngowsiri,Wannaporn Boripunt
International Journal of Marketing Studies , 2009, DOI: 10.5539/ijms.v1n2p151
Abstract: This research aims to study the behavior of using debit card and the quality perceiving on using debit card of the consumer in Songkhla province. Questionnaires were used for data collection by purposive sampling and equally distributed to 303 consumers who held at least 1 debit card and used it 3 months before the study. The collected data was analyzed using the descriptive statistics; frequency and percentage, and the inferential statistics; Pearson chi-square at the .05 level of statistics significance. This research found that the demographic characters of the consumers were female, between 21-30 years old, and held bachelor degree. Two-third of consumers were students, government and company officers. More than half of them earned 5,001 – 15,000 baht per month. The research also found that the consumers had only 1 debit card and most of them were Visa type. Most of their debit cards were issued by Bangkok Bank. They owned debit card for 1-2 years. Ratio of withdraw to payment is 40:60. They used debit card for withdrawing 2-3 times a month and 1,001 – 3,000 baht each time. They used debit card for expense 2-3 times a month and 500 – 1,000 baht each time. They frequently used their debit card at department store and paid for their infrastructures. Debit card made a little change on their expense behavior especially in food and drink consumption. This research also found that most of consumers well perceived on the service quality in all dimension of tangible, reliability, responsiveness, assurance, and empathy. This research found that service marketing mixes influenced on using debit card behavior are debit card, promotion, process, physical evidences, and productivity and quality control. The research also found that all service marketing mix influenced on quality perceiving on using debit card. The information from this research is beneficial to set debit card marketing mix strategy policy to stimulate consumer behavior on using debit card.
Cytokine gene expression in human hepatocytes infected with dengue virus serotype 3 (strain-16562)  [PDF]
Sutee Yoksan, Jundee Rabablert, Kumchol Chaiyo, Supoth Rajchakam, Supathra Tiewcharoen, Natthapol Rabablert, Soratorn Kerdkriangkrai, Narong Samngamnim, Watchara Phurttikul, Tarmphong Luangboribun
Health (Health) , 2013, DOI: 10.4236/health.2013.59206

Liver is a site of viral replication and liver dysfunction is a characteristic of severe dengue infection. To understand these mechanisms, we analyzed the response of a hepatic cell linage, HepG2 to infection with dengue 3 virus (strain 16562). Steady state levels of mRNA accumulation were assessed for 14 genes involved in modulation of the host immune responses, at 6, 24 and 48 hpi, by quantitative reverse transcription real-time PCR (qRT-PCR). Fourteen genes showed altered expression upon infection with D3V including; cytokines/chemokines (IL-1β, IL-6, IL-8, RANTES, MCP-2, IL-2Rα and TGF-βIIIR), type I interferon (IFN-α and IFN-β), and pattern-recognition receptors (TLR3, TLR8, RIG-1, MDA5 and MyD88). Although these genes are associated with mechanism of innate immune response and anti-viral activity, their altered expression does not inhibit D3V (strain 16562) growth kinetics and virus yield in HepG2 cells. Gene expression in liver may explain pathological changes associated with dengue virus infection.

Effects of Water Temperature on the White Spot Syndrome Virus Infection in Postlarvae Litopenaeus vannamei
Walailak Journal of Science and Technology , 2010, DOI: 10.2004/vol7iss2pp127-134
Abstract: This study evaluated the effects of high water temperature (32 ± 1 °C) on the white spot syndrome virus (WSSV) infection in Litopenaeus vannamei postlarvae (PL15). WSSV challenge was done by immersion. One group of PL15 was continuously maintained at 32 ± 1 °C until the end of the experiment after challenge and a control group of PL15 was constantly maintained at 28 ± 1 °C until the end of the experiment after challenge. Other groups were kept at 32 ± 1 °C until temperature was altered from 32 ± 1 °C to 28 ± 1 °C at 0, 1, 3, 5 and 7 days after infection. Gross signs and mortality were monitored every 12 h until the end of the experiment. WSSV infections were confirmed by nested-PCR, histopathology, immunohistochemistry and bioassay methods. Challenged shrimp were kept at 32 ± 1 °C for 0, 1, 3 and 5 days before the temperature was reduced to 28 ± 1 °C revealing that maintaining the temperature at 32 ± 1 °C for a longer period could delay clinical signs and onset of mortalities. Nevertheless, 100 % mortalities occurred in all groups and the control group within 7 days. All moribund PL15 were WSSV-positive by nested-PCR assay as well as histopathology, immunohistochemistry and bioassay methods. In contrast, PL15 constantly maintained at 32 ± 1 °C until the end of the experiment, and for 7 days after challenge before switching to 28 ± 1 °C did not show clinical signs and mortality. Surviving PL15 from both groups were WSSV-negative by nested-PCR assay as well as histopathology, immunohistochemistry and bioassay methods. This study clearly indicated that postlarvae maintained constantly at 32 ± 1 °C for 7 days were able to eliminate/clear WSSV infection.
Chikungunya Virus Infection of Cell Lines: Analysis of the East, Central and South African Lineage
Nitwara Wikan, Prirayapak Sakoonwatanyoo, Sukathida Ubol, Sutee Yoksan, Duncan R. Smith
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031102
Abstract: Chikungunya virus (CHIKV) is a re-emerging mosquito borne alphavirus that has caused large scale epidemics in the countries around the Indian Ocean, as well as leading to autochthonous transmission in some European countries. The transmission of the disease has been driven by the emergence of an African lineage of CHIKV with enhanced transmission and dissemination in Aedes mosquito hosts. Two main genotypes of this lineage have been circulating, characterized by the presence of a substitution of a valine for an alanine at position 226 of the E1 protein. The outbreak, numbering in millions of cases in the infected areas, has been associated with increasing numbers of cases with non-classical presentation including encephalitis and meningitis. This study sought to compare the original Ross strain with two isolates from the recent outbreak of chikungunya fever in respect of infectivity and the induction of apoptosis in eight mammalian cell lines and two insect cell lines, in addition to generating a comprehensive virus production profile for one of the newer isolates. Results showed that in mammalian cells there were few differences in either tropism or pathogenicity as assessed by induction of apoptosis with the exception of Hela cells were the recent valine isolate showed less infectivity. The Aedes albopictus C6/36 cell line was however significantly more permissive for both of the more recent isolates than the Ross strain. The results suggest that the increased infectivity seen in insect cells derives from an evolution of the CHIKV genome not solely associated with the E1:226 substitution.
Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses
Vu Ty Hang,Nguyen Minh Nguyet,Dinh The Trung,Vianney Tricou,Sutee Yoksan,Nguyen Minh Dung,Tran Van Ngoc,Tran Tinh Hien,Jeremy Farrar,Bridget Wills,Cameron P. Simmons
PLOS Neglected Tropical Diseases , 2009, DOI: 10.1371/journal.pntd.0000360
Abstract: Background Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. Methodology/Principal Findings The sensitivity and specificity of the Platelia NS1 ELISA assay and an NS1 lateral flow rapid test (LFRT) were compared against a gold standard reference diagnostic algorithm in 138 Vietnamese children and adults. Overall, the Platelia NS1 ELISA was modestly more sensitive (82%) than the NS1 LFRT (72%) in confirmed dengue cases. Both ELISA and LFRT assays were more sensitive for primary than secondary dengue, and for specimens collected within 3 days of illness onset relative to later time points. The presence of measurable DENV-reactive IgG and to a lesser extent IgM in the test sample was associated with a significantly lower rate of NS1 detection in both assays. NS1 positivity was associated with the underlying viraemia, as NS1-positive samples had a significantly higher viraemia than NS1-negative samples matched for duration of illness. The Platelia and NS1 LFRT were 100% specific, being negative in all febrile patients without evidence of recent dengue, as well as in patients with enteric fever, malaria, Japanese encephalitis and leptospirosis. Conclusions/Significance Collectively, these data suggest NS1 assays deserve inclusion in the diagnostic evaluation of dengue patients, but with due consideration for the limitations in patients who present late in their illness or have a concomitant humoral immune response.
Multi-Country Evaluation of the Sensitivity and Specificity of Two Commercially-Available NS1 ELISA Assays for Dengue Diagnosis
Maria G. Guzman ,Thomas Jaenisch,Roger Gaczkowski,Vo Thi Ty Hang,Shamala Devi Sekaran,Axel Kroeger,Susana Vazquez,Didye Ruiz,Eric Martinez,Juan C. Mercado,Angel Balmaseda,Eva Harris,Efren Dimano,Prisca Susan A. Leano,Sutee Yoksan,Elci Villegas,Herminia Benduzu,Iris Villalobos,Jeremy Farrar,Cameron P. Simmons
PLOS Neglected Tropical Diseases , 2010, DOI: 10.1371/journal.pntd.0000811
Abstract: Background Early diagnosis of dengue can assist patient triage and management and prevent unnecessary treatments and interventions. Commercially available assays that detect the dengue virus protein NS1 in the plasma/serum of patients offers the possibility of early and rapid diagnosis. Methodology/Principal Findings The sensitivity and specificity of the Pan-E Dengue Early ELISA and the Platelia? Dengue NS1 Ag assays were compared against a reference diagnosis in 1385 patients in 6 countries in Asia and the Americas. Platelia was more sensitive (66%) than Pan-E (52%) in confirmed dengue cases. Sensitivity varied by geographic region, with both assays generally being more sensitive in patients from SE Asia than the Americas. Both kits were more sensitive for specimens collected within the first few days of illness onset relative to later time points. Pan-E and Platelia were both 100% specific in febrile patients without evidence of acute dengue. In patients with other confirmed diagnoses and healthy blood donors, Platelia was more specific (100%) than Pan-E (90%). For Platelia, when either the NS1 test or the IgM test on the acute sample was positive, the sensitivity versus the reference result was 82% in samples collected in the first four days of fever. NS1 sensitivity was not associated to disease severity (DF or DHF) in the Platelia test, whereas a trend for higher sensitivity in DHF cases was seen in the Pan-E test (however combined with lower overall sensitivity). Conclusions/Significance Collectively, this multi-country study suggests that the best performing NS1 assay (Platelia) had moderate sensitivity (median 64%, range 34–76%) and high specificity (100%) for the diagnosis of dengue. The poor sensitivity of the evaluated assays in some geographical regions suggests further assessments are needed. The combination of NS1 and IgM detection in samples collected in the first few days of fever increased the overall dengue diagnostic sensitivity.
Evaluation of Commercially Available Diagnostic Tests for the Detection of Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM Antibody
Elizabeth A. Hunsperger ,Sutee Yoksan,Philippe Buchy,Vinh Chau Nguyen,Shamala Devi Sekaran,Delia A. Enria,Susana Vazquez,Elizabeth Cartozian,Jose L. Pelegrino,Harvey Artsob,Maria G. Guzman,Piero Olliaro,Julien Zwang,Martine Guillerm,Susie Kliks,Scott Halstead,Rosanna W. Peeling,Harold S. Margolis
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0003171
Abstract: Commercially available diagnostic test kits for detection of dengue virus (DENV) non-structural protein 1 (NS1) and anti-DENV IgM were evaluated for their sensitivity and specificity and other performance characteristics by a diagnostic laboratory network developed by World Health Organization (WHO), the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) and the Pediatric Dengue Vaccine Initiative (PDVI). Each network laboratory contributed characterized serum specimens for the panels used in the evaluation. Microplate enzyme-linked immunosorbent assay (ELISA) and rapid diagnostic test (RDT formats) were represented by the kits. Each ELISA was evaluated by 2 laboratories and RDTs were evaluated by at least 3 laboratories. The reference tests for IgM anti-DENV were laboratory developed assays produced by the Armed Forces Research Institute for Medical Science (AFRIMS) and the Centers for Disease Control and Prevention (CDC), and the NS1 reference test was reverse transcriptase polymerase chain reaction (RT-PCR). Results were analyzed to determine sensitivity, specificity, inter-laboratory and inter-reader agreement, lot-to-lot variation and ease-of-use. NS1 ELISA sensitivity was 60–75% and specificity 71–80%; NS1 RDT sensitivity was 38–71% and specificity 76–80%; the IgM anti-DENV RDTs sensitivity was 30–96%, with a specificity of 86–92%, and IgM anti-DENV ELISA sensitivity was 96–98% and specificity 78–91%. NS1 tests were generally more sensitive in specimens from the acute phase of dengue and in primary DENV infection, whereas IgM anti-DENV tests were less sensitive in secondary DENV infections. The reproducibility of the NS1 RDTs ranged from 92-99% and the IgM anti-DENV RDTs from 88–94%.
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