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Search Results: 1 - 10 of 107 matches for " Susann Patschan "
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Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis
Susann Patschan,Daniel Patschan,Elvira Henze,Sabine Blaschke,Johannes T. Wessels,Gerhard Anton Müller
International Journal of Nephrology , 2012, DOI: 10.1155/2012/715049
Abstract: Background. Granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) are autoimmune-mediated diseases characterized by vasculitic inflammation of respiratory tract and kidneys. Clinical observations indicated a strong association between disease activity and serum levels of certain types of autoantibodies (antineutrophil cytoplasm antibodies with cytoplasmic [cANCA in GPA] or perinuclear [pAN CA in MPA] immunofluorescence). Pathologically, both diseases are characterized by severe microvascular endothelial cell damage. Early endothelial outgrowth cells (eEOCs) have been shown to be critically involved in neovascularization under both physiological and pathological condition. Objectives. The principal aims of our study were (i) to analyze the regenerative activity of the eEOC system and (ii) to determine mPR3 and MPO expression in myelo monocytic cells with endothelial characteristics in GPA and MPA patients. Methods. In 27 GPA and 10 MPA patients, regenerative activity blood-derived eEOCs were analyzed using a culture-forming assay. Flk-1+, CD133+/Flk-1+, mPR3+, and Flk-1+/mPR3+ myelomonocytic cells were quantified by FACS analysis. Serum levels of Angiopoietin-1 and TNF- were measured by ELISA. Results. We found reduced eEOC regeneration, accompanied by lower serum levels of Angiopoietin-1 in GPA patients as compared to healthy controls. In addition, the total numbers of Flk-1+ myelomonocytic cells in the peripheral circulation were decreased. Membrane PR3 expression was significantly higher in total as well as in Flk-1+ myelomonocytic cells. Expression of MPO was not different between the groups. Conclusions. These data suggest impairment of the eEOC system and a possible role for PR3 in this process in patients suffering from GPA. 1. Introduction Granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) are autoimmune diseases characterized by systemic necrotizing vasculitis mainly affecting the respiratory tract and the kidneys [1]. Histopathological analysis reveals severe structural alterations of microvascular endothelial cells, leading to impaired microcirculation in skin, joints, respiratory tract, and kidneys, respectively. Endothelial damage has been suggested to result from interactions between primed neutrophils and ANCA with the endothelium followed by endothelial detachment from the basement membrane [2]. A number of different studies showed increased levels of both, circulating mature endothelial cells and endothelial microparticles in GPA and MPA [3, 4]. Thus, patients with GPA and MPA suffer from
Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)
Susann A Patschan, Daniel Patschan, Johanna Temme, Peter Korsten, Johannes T Wessels, Michael Koziolek, Elvira Henze, Gerhard A Müller
Critical Care , 2011, DOI: 10.1186/cc10100
Abstract: Patients with newly diagnosed sepsis were recruited from the ICU in a nonrandomized prospective manner. Blood samples were obtained within the first 12 hours after the diagnosis of sepsis. For quantifying endothelial progenitor cells (EPCs), CD133+/Flk-1+ cells were enumerated by cytometric analysis. Analysis of EPC proliferation was performed by a colony-forming units (CFU) assay. Blood concentrations of proangiogenic mediators were measured by ELISA. Acute renal dysfunction was diagnosed according to the Acute Kidney Injury Network (AKIN) criteria. Depending on the overall mean creatinine concentration during the stay at the ICU, patients were either assigned to a 'normal creatinine group' or to a 'high creatinine group'. Survival rates, frequency of dialysis, the simplified acute physiology score (SAPS) II scores, and different laboratory parameters were collected/used for further clinical characterizationCirculating EPCs were significantly higher in all sepsis patients included in the study as opposed to healthy controls. Patients within the 'high creatinine group' showed an even more pronounced EPC increase. In contrast, EPC proliferation was severely affected in sepsis. Neither total circulating EPCs nor EPC proliferation differed between patients requiring dialysis and patients without renal replacement therapy. Cell numbers and cell proliferation also did not differ between surviving patients and patients with sepsis-related death. Serum levels of vascular endothelial growth factor (VEGF), stromal derived factor-1 (SDF-1), and Angiopoietin-2 were higher in sepsis than in healthy controls. Sepsis patients within the 'high creatinine group' showed significantly higher mean serum levels of uric acid.Sepsis significantly affects the endothelial progenitor cell system, as reflected by increased EPC numbers, increased concentrations of proangiogenic mediators, and reduced proliferative capacity of the cells. This occurs independently from the frequency of dialysis
Inflammation and Microvasculopathy in Renal Ischemia Reperfusion Injury
Daniel Patschan,S. Patschan,G. A. Müller
Journal of Transplantation , 2012, DOI: 10.1155/2012/764154
Abstract: Acute renal failure (ARF) severely worsens prognosis of hospitalized patients. The most frequent cause of intrarenal ARF is transient or prolonged renal hypoperfusion (ischemia). Ischemia primarily affects the function and structure of tubular epithelial cells, which, in severe cases, is characterized by epithelial cell necrosis. Nevertheless, ischemia does not exclusively lead to alterations of epithelial cells but also causes interstitial inflammation and interstitial microvasculopathy. Both inflammation and microvasculopathy are particularly important in terms of postischemic kidney repair. Postischemic microvasculopathy is characterized by endothelial cell swelling with subsequent microvascular occlusion. Thus, reperfusion is inhibited (no-reflow phenomenon). Such endothelial cell dysfunction offers new therapeutic perspectives in ischemic ARF. Newer observations point towards the role of the so-called endothelial progenitor cells (EPCs) in the treatment of ARF. Systemic administration of EPCs to mice with bilateral renal ischemia mitigates postischemic endothelial cell dysfunction and protects animals from acute renal failure.
Endothelial Progenitor Cells in Acute Ischemic Kidney Injury: Strategies for Increasing the Cells' Renoprotective Competence
D. Patschan,S. Patschan,G. A. Müller
International Journal of Nephrology , 2011, DOI: 10.4061/2011/828369
Abstract: Acute ischemic kidney injury is the most frequent cause of acute renal failure in daily clinical practice. It has become increasingly recognized that microvascular endothelial cell dysfunction (ED) in peritubular capillaries inhibits the process of postischemic renal reperfusion. ED can serve as therapeutic target in the management of acute ischemic kidney injury. Postischemic reflow can be restored by systemic administration of either mature endothelial cells or of endothelial progenitor cells. Endothelial progenitor cells EPCs can be cultured from the peripheral circulation of humans and different animals. The cells act vasoprotectively by direct and indirect mechanisms. The protective effects of EPCs in acute ischemic kidney injury can be stimulated by preincubating the cells with different agonistic mediators. This paper summarizes the currently available data on strategies to improve the renoprotective activity of EPCs in acute ischemic kidney injury. 1. Endothelial Dysfunction in Acute Ischemic Kidney Injury Acute ischemic kidney injury is the most frequent cause of acute renal failure in daily clinical practice [1]. Despite the significant progress that has been made in the fields of intensive care medicine and renal replacement therapy over the past 30 years, the mortality of acute renal failure in hospitalized patients still varies from 30 to 70% [1–3]. Although hypoperfusion of the kidney, resulting from various pathologic conditions, primarily affects the function and structure of tubular epithelial cells, it has become increasingly recognized that microvascular endothelial cell dysfunction (ED) in peritubular capillaries inhibits the process of postischemic renal reperfusion and thereby prolongs kidney malfunction [4, 5]. First studies came from Flores et al. [6]: rats that underwent transient renal artery clamping showed intense swelling of all cellular elements in the kidney, leading to persistent renal hypoperfusion after the end of ischemia. Such no-reflow, which was in part also attributable to endothelial cell swelling, could effectively be treated by the injection of hypertonic mannitol solution, but remained unaffected by an equivalent expansion of the extracellular fluid volume with either isotonic saline or isotonic mannitol [7]. Further studies, published in 2001, showed that ischemia associated endothelial cell dysfunction in addition can result in permanent damage to peritubular capillaries. This damage worsens long-term outcome of kidney function [8, 9]. These data suggested that postischemic renal ED could potentially serve as
Inflammation and Microvasculopathy in Renal Ischemia Reperfusion Injury
Daniel Patschan,S. Patschan,G. A. Müller
Journal of Transplantation , 2012, DOI: 10.1155/2012/764154
Abstract: Acute renal failure (ARF) severely worsens prognosis of hospitalized patients. The most frequent cause of intrarenal ARF is transient or prolonged renal hypoperfusion (ischemia). Ischemia primarily affects the function and structure of tubular epithelial cells, which, in severe cases, is characterized by epithelial cell necrosis. Nevertheless, ischemia does not exclusively lead to alterations of epithelial cells but also causes interstitial inflammation and interstitial microvasculopathy. Both inflammation and microvasculopathy are particularly important in terms of postischemic kidney repair. Postischemic microvasculopathy is characterized by endothelial cell swelling with subsequent microvascular occlusion. Thus, reperfusion is inhibited (no-reflow phenomenon). Such endothelial cell dysfunction offers new therapeutic perspectives in ischemic ARF. Newer observations point towards the role of the so-called endothelial progenitor cells (EPCs) in the treatment of ARF. Systemic administration of EPCs to mice with bilateral renal ischemia mitigates postischemic endothelial cell dysfunction and protects animals from acute renal failure. 1. Introduction Acute renal failure (ARF) severely worsens prognosis of hospitalized patients. Approximately 1–5% of all patients treated in the hospital develop ARF [1]. The clinical significance of ARF results from its high mortality, which still today ranges from 30 to 70% [2]. ARF is categorized depending on its primary cause. Prerenal failure results from transient renal hypoperfusion. It is a functional response of a structurally intact kidney to hypoperfusion [3]. While postrenal ARF is caused by urinary tract obstruction with or without subsequent damage of renal tissue, intrinsic or intrarenal ARF is caused by diseases that either affect the glomeruli, the vasculature, the interstitium, or the tubules. The difference between prerenal and intrarenal failure due to hypoperfusion lies in the presence of structural tubular damage in the latter. The most frequent cause of intrarenal ARF in hospitalized patients is transient or prolonged renal hypoperfusion (ischemia reperfusion injury—IRI) [4–6]. Renal IRI is of particular importance in the setting of kidney transplantation [7]. Ischemia primarily affects the function and structure of tubular epithelial cells, which, in severe cases, is characterized by epithelial cell necrosis [8]. Nevertheless, IRI does not exclusively lead to alterations of epithelial cell function and structure but also causes interstitial inflammation and interstitial microvasculopathy (Figure 1).
Design as Language – a Misconception? Design as Language – a Misconception?
Susann Vihma
FORMakademisk , 2012,
Abstract: Because design is often conceived of as a language, it is worthwhile to take a closer look at this relationship. It is especially interesting to look at the transition from the verbal to the visual in interpretations and analyses of design. This article discusses some salient topics appearing in this context. In particular, claims made in the books by Kress and van Leeuwen (1996) and Krippendorff (2006) are discussed, because their approaches are widely used in design discourses, in journals, schools, exhibition catalogues, etc. These texts probably influence design research, but, in the present author’s view, do not help interpretation of visual qualities in design. The underlying critical assumption in this article is that the language metaphor and concepts derived from linguistics do not do justice to the interpretations of design objects, especially concerning their visual qualities. When these qualities are underrated, it seems difficult to assess the cultural significance of design. Because design is often conceived of as a language, it is worthwhile to take a closer look at this relationship. It is especially interesting to look at the transition from the verbal to the visual in interpretations and analyses of design. This article discusses some salient topics appearing in this context. In particular, claims made in the books by Kress and van Leeuwen (1996) and Krippendorff (2006) are discussed, because their approaches are widely used in design discourses, in journals, schools, exhibition catalogues, etc. These texts probably influence design research, but, in the present author’s view, do not help interpretation of visual qualities in design. The underlying critical assumption in this article is that the language metaphor and concepts derived from linguistics do not do justice to the interpretations of design objects, especially concerning their visual qualities. When these qualities are underrated, it seems difficult to assess the cultural significance of design.
Empirisch begründete Typenbildung in der qualitativen Sozialforschung Empirically Grounded Construction of Types and Typologies in Qualitative Social Research La construcción empírica de tipos en la investigación cualitativa
Susann Kluge
Forum : Qualitative Social Research , 2000,
Abstract: In der qualitativen Sozialforschung finden sich nur wenige Ans tze, in denen der Proze der Typenbildung detailliert expliziert und systematisiert wird; au erdem werden sehr unterschiedliche Typenbegriffe (wie z.B. Idealtypen, Realtypen, Prototypen, Extremtypen, Strukturtypen etc.) verwendet. Da der Typusbegriff für die qualitative Sozialforschung von zentraler Bedeutung ist, ist eine Kl rung des Begriffs sowie des Prozesses der Typenbildung jedoch dringend erforderlich. In dem Beitrag wird daher zun chst eine allgemeine Definition des Typusbegriffs vorgelegt, von der ausgehend Regeln für eine systematische und nachvollziehbare Bildung von Typen und Typologien formuliert werden. URN: urn:nbn:de:0114-fqs0001145 In qualitative social research, there are only a few approaches in which the process of typology construction is explicated and systematised in detail; furthermore, you can find very different concepts of types like ideal types, real types, prototypes, extreme types, types of structure. Because the construction of typologies is of central importance for the qualitative social research, it is necessary to clarify the concept of types and the process of typology construction. Therefore, this article will first present a general definition of the concept of types, and then how this definition forms the basis of rules for a systematic and controlled construction of types and typologies. URN: urn:nbn:de:0114-fqs0001145 URN: urn:nbn:de:0114-fqs0001145
GMA-Jahrestagung in Bochum vom 23.-25. September 2010 []
Enigk, Susann
GMS Zeitschrift für Medizinische Ausbildung , 2010, DOI: 10.3205/zma000688
Abstract:
La industria maquiladora y la migración interna en México
Hjorth Boisen,Susann Vallentin;
Gaceta Laboral , 2009,
Abstract: the objective of this article is to analyze the relationship between the development of the assembly plant export industry [industria maquiladora de exportación (ime)], the generation of migratory flow from the southern veracruz toward the city of juarez, beginning in 1995, and some of the characteristics and specific dynamics of this labor migration and its relationship to local labor markets. the methodology was extended fieldwork during two periods: the year 2000 and between 2002 and 2006. the techniques used were primarily semi-structured, in-depth interviews and participant observation. results point toward a close link between the development of the assembly plant industry and the evolution of migratory flow, in a significant interaction with social networks and family migratory strategies. conclusions are that a close connection exists between the process of productive relocation and displacement of the labor force toward the assembly plant area in northern mexico, generating important bonds between local labor markets in the assembly plant zone on the northern mexican frontier and local labor markets in the south of veracruz.
Tagungsessay: Diskursforschung im Prozess ihrer Etablierung Conference Essay: The Field of Discourse Research in the Process of Becoming Established Ensayo: La investigación del discurso en proceso de consolidarse
Susann Fegter,Antje Langer
Forum : Qualitative Social Research , 2008,
Abstract: In der letzten Dekade hat sich die Diskursforschung im Anschluss an Michel FOUCAULT im deutschsprachigen Raum interdisziplin r best ndig weiterentwickelt. Sie ist dabei, sich im Rahmen qualitativer Sozialforschung – wie auch an sprachwissenschaftlichen Verfahren orientiert – zu etablieren. Auf der internationalen und interdisziplin ren Tagung "Sprache – Macht – Wissen" vom 10.-12.Oktober 2007 in Augsburg wurde der aktuelle Stand von Diskurstheorie und -analyse eruiert und diskutiert. Der Tagungsessay soll einen Einblick in die derzeitige Diskussion geben. Wir zeichnen zun chst die Fragestellungen und Zielsetzungen der Tagung nach. Es folgt eine knappe Zusammenfassung der gehaltenen Vortr ge. Im Laufe der Tagung kristallisierten sich verschiedene Schwerpunkte heraus, die wiederholt aufgegriffen und diskutiert wurden: das Verh ltnis von Diskursanalyse und Kritik, das Verh ltnis von Subjekt(ivit t) und Diskurs, das Verh ltnis von Macht, Diskurs und Dispositiv sowie das Verh ltnis von Diskursanalyse und Visualit t. Mit der Systematisierung dieser vier Punkte nehmen wir eine kritische Betrachtung der "Ergebnisse" der Tagung vor. Abschlie end verweisen wir auf zwei aktuelle Netzwerkinitiativen zur interdisziplin ren Diskursforschung, die w hrend der Tagung vorgestellt wurden. URN: urn:nbn:de:0114-fqs0802181 Over the last decade, interdisciplinary discourse research in the tradition of Michel FOUCAULT has been evolving constantly in the German-speaking world. It also takes linguistic methodologies as a point of orientation and establishes itself in the framework of qualitative social research. At the international interdisciplinary conference "Language—Power—Knowledge" in Augsburg from 10 – 12 October 2007, participants explored and discussed the current state of discourse theory and analysis. With the present conference essay, we intend to provide insight into the discussions that took place there. We first outline the questions and goals of the conference, and then present a brief summary of the lectures. A number of focal points emerged at the conference that were taken up repeatedly and discussed in a variety of contexts: the relation between discourse analysis and critique, the relation between the subject (subjectivity) and discourses, the relation between power, discourse and dispositive, and the relation between discourse analyses and visuality. In systematizing these four points, we undertake a critical consideration of the "results" of the conference. In conclusion, we highlight two current network initiatives on interdisciplinary discourse research t
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