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Search Results: 1 - 10 of 3846 matches for " Susana Larrucea "
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Involvement of platelet-tumor cell interaction in immune evasion. Potential role of podocalyxin-like protein 1
Laura Amo,Estibaliz Tamayo-Orbegozo,Cristina Eguizabal,Olatz Zenarruzabeitia,Jorge Monge,Miguel A. Vesga,Francisco Borrego,Susana Larrucea
Frontiers in Oncology , 2014, DOI: 10.3389/fonc.2014.00245
Abstract: Besides their essential role in hemostasis and thrombosis, platelets are involved in the onset of cancer metastasis by interacting with tumor cells. Platelets release secretory factors that promote tumor growth, angiogenesis, and metastasis. Furthermore, the formation of platelet-tumor cell aggregates in the bloodstream provides cancer cells with an immune escape mechanism by protecting circulating malignant cells from immune-mediated lysis by natural killer (NK) cells. Platelet-tumor cell interaction is accomplished by specific adhesion molecules, including integrins, selectins, and their ligands. Podocalyxin-like protein 1 (PCLP1) is a selectin ligand protein which overexpression has been associated with several aggressive cancers. PCLP1 expression enhances cell adherence to platelets in an integrin-dependent process and through the interaction with P-selectin expressed on activated platelets. However, the involvement of PCLP1-induced tumor-platelet interaction in tumor immune evasion still remains unexplored. The identification of selectin ligands involved in the interaction of platelets with tumor cells may provide help for the development of effective therapies to restrain cancer cell dissemination. This article summarizes the current knowledge on molecules that participate in platelet-tumor cell interaction as well as discusses the potential role of PCLP1 as a molecule implicated in tumor immune evasion.
Diminished Thrombogenic Responses by Deletion of the Podocalyxin Gene in Mouse Megakaryocytes
Miguel Pericacho, Sonia Alonso-Martín, Susana Larrucea, Consuelo González-Manchón, Darío Fernández, Inés Sánchez, Matilde S. Ayuso, Roberto Parrilla
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0026025
Abstract: Podocalyxin (Podxl) is a type I membrane sialoprotein of the CD34 family, originally described in the epithelial glomerular cells of the kidney (podocytes) in which it plays an important function. Podxl can also be found in megakaryocytes and platelets among other extrarenal places. The surface exposure of Podxl upon platelet activation suggested it could play some physiological role. To elucidate the function of Podxl in platelets, we generated mice with restricted ablation of the podxl gene in megakaryocytes using the Cre-LoxP gene targeting methodology. Mice with Podxl-null megakaryocytes did not show any apparent phenotypical change and their rates of growth, life span and fertility did not differ from the floxed controls. However, Podxl-null mice showed prolonged bleeding time and decreased platelet aggregation in response to physiological agonists. The number, size-distribution and polyploidy of Podxl-null megakaryocytes were similar to the floxed controls. Podxl-null platelets showed normal content of surface receptors and normal activation by agonists. However, the mice bearing Podxl-null platelets showed a significant retardation in the ferric chloride-induced occlusion of the carotid artery. Moreover, acute thrombosis induced by the i.v. injection of sublethal doses of collagen and phenylephrine produced a smaller fall in the number of circulating platelets in Podxl-null mice than in control mice. In addition, perfusion of uncoagulated blood from Podxl-null mice in parallel flow chamber showed reduced adhesion of platelets and formation of aggregates under high shear stress. It is concluded that platelet Podxl is involved in the control of hemostasis acting as a platelet co-stimulator, likely due to its pro-adhesive properties.
Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoter
Nora Butta, Susana Larrucea, Sonia Alonso, Ramón B Rodriguez, Elena G Arias-Salgado, Matilde S Ayuso, Consuelo González-Manchón, Roberto Parrilla
BMC Molecular Biology , 2006, DOI: 10.1186/1471-2199-7-17
Abstract: The promoter region of the human Podxl gene has been cloned and its structure and function were analyzed. The primary DNA sequence is rich in G+C and is devoid of TATA or CAAT boxes. The sequence contains recognition sites for several putative transcription factors; however, the basic promoter activity seems to rely entirely on Sp1 transcription factor since supershift analysis was positive only for this factor. The region encompassed by 66 to -111 nts conferred the minimal transcriptional activity that increases as the number of Sp1 sites augmented with the length of the promoter fragment. In Sp1-lacking insect cells the Podxl promoter constructs showed activity only if cotransfected with an Sp1 expression plasmid. Finally, mutation of the Sp1 sites reduced the promoter activity.We analyzed whether methylation of the CpG dinucleotides present in the first ~600 nts of the promoter region of Podxl could explain the variable rates of expression in different types of cells. Inactivation of methyltransferases by 5'-aza-2'deoxicitidine showed a dose-dependent increase in the podxl content. Moreover, in vitro methylation of the promoter constructs -111,-181 and -210 led to an almost complete reduction of the promoter activity. A correlation was found between the degree of methylation of the CpG promoter dinucleotides and the rate of podxl expression in different cell lines.Our results indicate that transcriptional regulation of Podxl is supported primarily by Sp1 site(s) and that DNA-methylation of the CpG promoter islands contributes to control the tissue specific expression of podxl.Podocalyxin (podxl) is an integral membrane protein originally identified by Kerjaschki et al [1] in the foot processes of the rat kidney glomerular podocytes. It was described as a sialoprotein of 140-kDa based on its staining with alcian blue. The high sulfate content of this protein contributes in part to its heavily negative charge [2] that is supposed to maintain open the interdigitatio
Recidiva del color dentario por té, café y vino: In vitro
Arévalo Pineda,M; Larrucea Verdugo,C;
Revista clínica de periodoncia, implantología y rehabilitación oral , 2012, DOI: 10.4067/S0719-01072012000200001
Abstract: this in vitro study established if teeth treated with dental bleaching have a higher change of color over time than those that aren’t treated, when subjected to three chromogenic beverages (coffee, tea and red wine). 45 healthy bovine incisors were used, maintained in 0.9% sodium chloride, at 37oc. every specimen was divided into 2 half; one half was subjected to dental bleaching with 35% hydrogen peroxide and the other was taken as control. the color was measured with the vita easyshade spectrophotometer. the specimens were randomly divided in groups of 15, and were immersed in coffee, tea and red wine for 10 minutes, 20 times, the value being recorded after each immersion. the data was analyzed with anova and tukey test, using 95% of confidence interval. results show that bleaching significantly modifies (p=0.05) the color in the three groups (gc=85.8 to 95.1; gt=87.4 to 97.3 y gv=90.8 to 99.3). color relapse in the three groups was observed along the 20 immersions. initial δe values (gc=18.89; gt=22.97; gv=56.46) were significantly different than final δe values (gc=5.56; gt=5.38; gv=12.49). the chromogenic beverages stained more the treated group because a higher δe units decrease was observed in this group (gccontrol=20.98-5.01; gtcontrol=17.11-3.66; gv control=54.62-11.49). the three chromogenic beverages produced color relapse in the bleaching teeth, being red wine the one causing more staining. it was concluded that the specimens treated subjected to three kinds of chromogenic, had higher change of color than control group, but at the end the treated group doesn’t get more stained than the control group.
Recidiva del color dentario por té, café y vino: In vitro Dental bleaching regression caused by chromogenic beverages: In vitro
M Arévalo Pineda,C Larrucea Verdugo
Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral , 2012,
Abstract: Este estudio, in vitro, determinó si los dientes con clareamiento presentan mayor cambio de color en el tiempo que los no tratados, al someterse a tinción con bebidas cromógenas, café, té y vino. Se utilizaron 45 incisivos sanos de bovino conservados en suero a 37oC. Cada espécimen se dividió en dos mitades, una sometida a clareamiento con peróxido de hidrógeno al 35% y otra control. Se midió color con Espectrofotómetro Vita EasyShade. Se dividieron los especímenes al azar en grupos de 15 y fueron sumergidos en café, té y vino, durante 10 minutos, 20 veces, registrando color después de cada inmersión. Los datos fueron analizados con ANOVA y Test de Tukey, con 95% de intervalo de confianza. Los resultados mostraron que, el clareamiento modifica significativamente (p=0.05) el color en los tres grupos (GC=85.8 a 95.1; GT=87.4 a 97.3 y GV=90.8 a 99.3), la recidiva de color se observa a lo largo de las 20 inmersiones, siendo significativa la diferencia de valores ΔE iniciales (GC=18.89; GT=22.97; GV=56.46) y finales (GC=5.56; GT=5.38; GV=12.49). El grupo tratado presenta mayor descenso de unidades ΔE a lo largo de las inmersiones, por lo que es el más te ido (GCcontrol=20.98-5.01; GTcontrol=17.11-3.66; GVcontrol=54.62-11.49). Las tres bebidas cromógenas causan recidiva de color en los dientes clareados, siendo el vino el que causa mayor tinción. Se concluyó que las piezas tratadas, sometidas a los tres tipos de cromógenos, tienen mayor cambio de color que las que no lo son, pero finalmente no se oscurecen más que las no tratadas. This in vitro study established if teeth treated with dental bleaching have a higher change of color over time than those that aren’t treated, when subjected to three chromogenic beverages (coffee, tea and red wine). 45 healthy bovine incisors were used, maintained in 0.9% sodium chloride, at 37oC. Every specimen was divided into 2 half; one half was subjected to dental bleaching with 35% hydrogen peroxide and the other was taken as control. The color was measured with the Vita EasyShade Spectrophotometer. The specimens were randomly divided in groups of 15, and were immersed in coffee, tea and red wine for 10 minutes, 20 times, the value being recorded after each immersion. The data was analyzed with ANOVA and Tukey test, using 95% of confidence interval. Results show that bleaching significantly modifies (p=0.05) the color in the three groups (GC=85.8 to 95.1; GT=87.4 to 97.3 y GV=90.8 to 99.3). Color relapse in the three groups was observed along the 20 immersions. Initial ΔE values (GC=18.89; GT=22.97; GV=56.46) were significant
Detección de incrementos de Dimensión Vertical Oclusal mediante análisis cefalométrico de Ricketts
Carrera Vidal,C; Larrucea Verdugo,C; Galaz Valdés,C;
Revista clínica de periodoncia, implantología y rehabilitación oral , 2010, DOI: 10.4067/S0719-01072010000200004
Abstract: introduction: oral rehabilitation of patients with severe wear, becomes complex when there is a need to increase vertical dimension. evaluation methods of vertical dimension do not deliver accurate information to define as increase is necessary. the cephalometric analysis appears as an additional option to the clinical tools, specific ricketts analysis containing vertical measurements of the lower third, through the lower facial height angle, whose normal value is 47o, but with a high-standard deviation. objective: to determine whether the lower height ricketts angle is sensitive to increases in oclusal vertical dimension (ovd). method: nine subjects aged 20 to 30 years-old underwent cephalometric analysis in order to measure the lower height ricketts angle in centric occlusion position, then increasing ovd in 1, 3 and 5 mm. results: lower height ricketts angle showed an increase as ovd of 1, 3 and 5 mm augmented in all subjects. nonparametric friedman′s statistical analysis was applied, being the results statistically significant (p=0.000). according to post hoc test of siegel and castellon, lower height ricketts angle variation of 1 mm is not statistically significant. conclusions: it may be suggested that the ricketts cephalometric analysis by measuring the lower height angle is able to detect increases in the ovd, but the change that occurs within 1 mm increase is not statistically significant.
Detección de incrementos de Dimensión Vertical Oclusal mediante análisis cefalométrico de Ricketts Detection of increases of Vertical Occlusal Dimension by Ricketts cephalometric analysis
C Carrera Vidal,C Larrucea Verdugo,C Galaz Valdés
Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral , 2010,
Abstract: Introducción: La rehabilitación de pacientes con desgastes severos es compleja cuando se determina un aumento de Dimensión Vertical. Los métodos de evaluación de Dimensión Vertical no entregan una información precisa para definir cuánto incremento es necesario. El análisis cefalométrico aparece como una opción adicional a las herramientas clínicas, específicamente el análisis de Ricketts contiene mediciones verticales del tercio inferior, a través del ángulo Altura Facial Inferior, cuyo valor normal es de 47o, pero con una desviación estándar alta. Objetivo: Determinar si el ángulo Altura Inferior de Ricketts es sensible a incrementos de Dimensión Vertical Oclusal (DVO). Método: A nueve sujetos entre 20 y 30 a os se les realizó un análisis cefalométrico con el fin de medir el ángulo Altura Inferior de Ricketts, en posición MIC, y luego con incrementos de la Dimensión Vertical de 1, 3 y 5 mm. Resultados: Los resultados mostraron que el valor del ángulo Altura Inferior de Ricketts (AFI) aumentó con los incrementos de la Dimensión Vertical Oclusal de 1, 3 y 5 mm en todos los sujetos. Al análisis estadístico no paramétrico de Friedman, estos resultados son significativos (p=0.000). A la prueba Post Hoc de Siegel y Castellon la variación del ángulo Altura Inferior de Ricketts con el incremento de 1 mm de DVO no es significativa. Conclusiones: Se puede sugerir que el análisis cefalométrico de Ricketts, a través de la medición del ángulo AFI, es capaz de detectar incrementos en la DVO, pero la variación que se produce con 1 mm de incremento no es estadísticamente significativa. Introduction: Oral rehabilitation of patients with severe wear, becomes complex when there is a need to increase Vertical Dimension. Evaluation methods of Vertical Dimension do not deliver accurate information to define as increase is necessary. The cephalometric analysis appears as an additional option to the clinical tools, specific Ricketts analysis containing vertical measurements of the lower third, through the Lower Facial Height angle, whose normal value is 47o, but with a high-standard deviation. Objective: To determine whether the Lower Height Ricketts angle is sensitive to increases in Oclusal Vertical Dimension (OVD). Method: Nine subjects aged 20 to 30 years-old underwent cephalometric analysis in order to measure the Lower Height Ricketts angle in centric occlusion position, then increasing OVD in 1, 3 and 5 mm. Results: Lower Height Ricketts angle showed an increase as OVD of 1, 3 and 5 mm augmented in all subjects. Nonparametric Friedman′s statistical analysis was applied
CPMD simulation of Cu2+ -- phenylalanine complex under micro-solvated environment
Aravindhan Ganesan,Jens Dreyer,Feng Wang,Jaakko Akola,Julen Larrucea
Physics , 2013,
Abstract: The study combines DFT calculations and CPMD simulations to investigate the structures of phenylalanine-copper (II) ([Phe-Cu]2+) complexes and the micro-solvation processes. ....It is found that the phenylalanine moiety appears to be in the neutral form in isolated and mono-hydrated complexes, but in the zwitterionic form in other hydrated complexes (with n no less than 2). .... The present CPMD simulations reveal that the maximum coordination of Cu2+ in the presence of the Phe ligand does not exceed four: the oxygen atoms from three water molecules and one carboxyl oxygen atom of Phe. Any excess water molecules will migrate to the second solvation shell. Moreover a unique structural motif is present in the lowest energy complexes, which is recognized to be significant in stabilizing the structures of the complexes. .....
A Matrix Approach to the Socioeconomic Activity of a Country  [PDF]
Susana Santos
Theoretical Economics Letters (TEL) , 2018, DOI: 10.4236/tel.2018.85075
Abstract: A Social Accounting Matrix (SAM) is presented as a tool to study the socioeconomic activity of a country. This activity involves the monetary or nominal flows that are measured by the National Accounts, as well as production (organized in factors, industries and goods and services) and institutions (organized in households, general government, non-financial and financial corporations, non-profit institutions serving households, and rest of the world). In order to contribute to the definition of a methodology that can improve the knowledge of the different aspects of this activity, the potentialities of a SAM for its reading and interpreting are explored, as well as for carrying out experiments regarding its functioning. Through a SAM-based approach, how to construct more or less complex networks of linkages of the above mentioned flows is shown, from which structural features can be evidenced and the associated multiplier effects studied. Following an application to Portugal, it is shown that a numerical version of a SAM, enables an empirical description of the origin, use, and distribution of income, whereas, an algebraic version of a SAM allows one to carry out, for example, a deeper study of the multiplier effects associated with the institutional distribution of income. The crucial role of the factors of production accounts is identified in this study, namely when they establish the link between the generation and the distribution and use of income. In this process, the important role the complementary details that the Input-Output Matrix (IOM) can add is also identified. Thus, being the generation of income, the result of the output of goods and services and the associated costs, on the one hand, an industry by industry IOM can add details regarding domestic and imported intermediate consumption by and between industries and, on the other hand, a product by product IOM can add details regarding the domestic and imported intermediate consumption of goods and services.
Child’s Life, Step-Family and Decision-Making Process  [PDF]
Susana Navas Navarro
Beijing Law Review (BLR) , 2013, DOI: 10.4236/blr.2013.42008
Abstract:

Step-families are included within the concept of “family” used by both Art. 8 of the Rome Convention for the protection of Human Rights and Fundamental Freedoms and Art. 7 of the European Charter of Fundamental Rights of 2010. According to the OECD and EUROSTAT figures children that are living in step-families are between 8 and 12 years old, that is, they could be considered as preadolescents or in some cases adolescents at all. Thus, they are individuals with a certain grade of maturity that allow them to form their own views and express them. Today, there is an inevitable link between family and children’s participation rights (Art. 12 CRC; Art. 24 EU Charter). Nevertheless, European Law refers to the step-family from the “adult’s rights” viewpoint concerning the assignment of the parental responsibility to the step-parent neglecting children’s perspective. As said by Art. 6(1) EU Treaty of Lisbon and by Art. 52(2) EU Charter, the last legal instrument has the same legal recognition as the EU Treaties, which implies that the bedrock for direct and vertical application of the fundamental rights of children by both the EUCJ and national Courts is settled. Since 2006 one of the strategic objectives of the communitarian institutions is prioritizing the effective implementation of children’s rights.

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