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Search Results: 1 - 10 of 5142 matches for " Susan Grass "
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The Haemophilus influenzae HMW1C Protein Is a Glycosyltransferase That Transfers Hexose Residues to Asparagine Sites in the HMW1 Adhesin
Susan Grass,Cheryl F. Lichti,R. Reid Townsend,Julia Gross,Joseph W. St. Geme III
PLOS Pathogens , 2010, DOI: 10.1371/journal.ppat.1000919
Abstract: The Haemophilus influenzae HMW1 adhesin is a high-molecular weight protein that is secreted by the bacterial two-partner secretion pathway and mediates adherence to respiratory epithelium, an essential early step in the pathogenesis of H. influenzae disease. In recent work, we discovered that HMW1 is a glycoprotein and undergoes N-linked glycosylation at multiple asparagine residues with simple hexose units rather than N-acetylated hexose units, revealing an unusual N-glycosidic linkage and suggesting a new glycosyltransferase activity. Glycosylation protects HMW1 against premature degradation during the process of secretion and facilitates HMW1 tethering to the bacterial surface, a prerequisite for HMW1-mediated adherence. In the current study, we establish that the enzyme responsible for glycosylation of HMW1 is a protein called HMW1C, which is encoded by the hmw1 gene cluster and shares homology with a group of bacterial proteins that are generally associated with two-partner secretion systems. In addition, we demonstrate that HMW1C is capable of transferring glucose and galactose to HMW1 and is also able to generate hexose-hexose bonds. Our results define a new family of bacterial glycosyltransferases.
Houellebecq and the Novel as Site of Epistemic Rebellion
Delphine Grass
Opticon1826 , 2006, DOI: 10.5334/opt.010609
Abstract: Michel Houellebecq is a writer who cultivates an incongruous literary garden, where the necessity of describing contemporary objects as equipment for the manufacture of individuals can be said to precede his concern for literary conventions. This has been at the centre of much criticism formulated around Houellebecq’s novels. One book in particular by Eric Naulleau called Au Secour, Houellebecq Revient! attacks his novels on the grounds that their success relies on his charming the reader by using recognisable elements of everyday life and incorporating them in the novels, in such a way that they could no longer be distinguished from the world of everyday life.
Jatropha curcas L.: Visions and Realities
Martin Grass
Journal of Agriculture and Rural Development in the Tropics and Subtropics , 2009,
Abstract: Since several years Jatropha is experiencing a renaissance. The main drivers for this development are the biofuel boom in general and the special attributes of Jatropha itself. This paper discusses the current knowledge as well as expectations of Jatropha and the consequential outcomes starting with data availability and quality followed by economic and political needs and constraints.
From 0D to 1D spatial models using OCMat
Dieter Grass
Quantitative Finance , 2015,
Abstract: We show that the standard class of optimal control models in OCMat can be used to analyze 1D spatial distributed systems. This approach is an intermediate step on the way to the FEM discretization approach presented in Grass and Uecker (2015). Therefore, the spatial distributed model is transformed into a standard model by a finite difference discretization. This (high dimensional) standard model is then analyzed using OCMAT. As an example we apply this method to the spatial distributed shallow lake model formulated in Brock and Xepapadeas (2008). The results are then compared with those of the FEM discretization in GRass and Uecker (2015)
The Actinobacillus pleuropneumoniae HMW1C-Like Glycosyltransferase Mediates N-Linked Glycosylation of the Haemophilus influenzae HMW1 Adhesin
Kyoung-Jae Choi,Susan Grass,Seonghee Paek,Joseph W. St. Geme III,Hye-Jeong Yeo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0015888
Abstract: The Haemophilus influenzae HMW1 adhesin is an important virulence exoprotein that is secreted via the two-partner secretion pathway and is glycosylated at multiple asparagine residues in consensus N-linked sequons. Unlike the heavily branched glycans found in eukaryotic N-linked glycoproteins, the modifying glycan structures in HMW1 are mono-hexoses or di-hexoses. Recent work demonstrated that the H. influenzae HMW1C protein is the glycosyltransferase responsible for transferring glucose and galactose to the acceptor sites of HMW1. An Actinobacillus pleuropneumoniae protein designated ApHMW1C shares high-level homology with HMW1C and has been assigned to the GT41 family, which otherwise contains only O-glycosyltransferases. In this study, we demonstrated that ApHMW1C has N-glycosyltransferase activity and is able to transfer glucose and galactose to known asparagine sites in HMW1. In addition, we found that ApHMW1C is able to complement a deficiency of HMW1C and mediate HMW1 glycosylation and adhesive activity in whole bacteria. Initial structure-function studies suggested that ApHMW1C consists of two domains, including a 15-kDa N-terminal domain and a 55-kDa C-terminal domain harboring glycosyltransferase activity. These findings suggest a new subfamily of HMW1C-like glycosyltransferases distinct from other GT41 family O-glycosyltransferases.
Juhani Pallasmaa
Grass,Diego; Jadue,Javiera;
ARQ (Santiago) , 2010, DOI: 10.4067/S0717-69962010000300015
Abstract: en la última edición de este a?o 2010, completamos la entrega de tres entrevistas realizadas por la editorial 0300tv a curadores y editores del mundo de la arquitectura y el urbanismo. al igual que las publicaciones anteriores, esta entrevista ha sido editada en conjunto con arq con la intención de dar a conocer parte de este valioso material en un formato distinto al audiovisual, que suele ser la norma para 0300tv.
Juhani Pallasmaa
Diego Grass,Javiera Jadue
ARQ , 2010,
Abstract: En la última edición de este a o 2010, completamos la entrega de tres entrevistas realizadas por la editorial 0300TV a curadores y editores del mundo de la arquitectura y el urbanismo. Al igual que las publicaciones anteriores, esta entrevista ha sido editada en conjunto con ARQ con la intención de dar a conocer parte de este valioso material en un formato distinto al audiovisual, que suele ser la norma para 0300TV.
Carving and living
Pau Baya Grass
Coolabah , 2009,
Abstract: Barcelona, 1974.He studied at the University of Barcelona,specialising in sculpture, finishing his degreein arts in 2000. He is an art teacher in a privatesecondary school in Barcelona.He has won prizes for his work and hasworked with Italian architects as well asexhibiting his work in Catalunya.
Mesoscale modelling of polyelectrolyte electrophoresis
Kai Grass,Christian Holm
Physics , 2009, DOI: 10.1039/b902011j
Abstract: The electrophoretic behaviour of flexible polyelectrolyte chains ranging from single monomers up to long fragments of hundred repeat units is studied by a mesoscopic simulation approach. Abstracting from the atomistic details of the polyelectrolyte and the fluid, a coarse-grained molecular dynamics model connected to a mesoscopic fluid described by the Lattice Boltzmann approach is used to investigate free-solution electrophoresis. Our study demonstrates the importance of hydrodynamic interactions for the electrophoretic motion of polyelectrolytes and quantifies the influence of surrounding ions. The length-dependence of the electrophoretic mobility can be understood by evaluating the scaling behavior of the effective charge and the effective friction. The perfect agreement of our results with experimental measurements shows that all chemical details and fluid structure can be safely neglected, and a suitable coarse-grained approach can yield an accurate description of the physics of the problem, provided that electrostatic and hydrodynamic interactions between all entities in the system, i.e., the polyelectrolyte, dissociated counterions, additional salt and the solvent, are properly accounted for. Our model is able to bridge the single molecule regime of a few nm up to macromolecules with contour lengths of more than 100 nm, a length scale that is currently not accessible to atomistic simulations.
Polyelectrolytes in electric fields: Measuring the dynamical effective charge and effective friction
Kai Grass,Christian Holm
Physics , 2008,
Abstract: We use a coarse-grained molecular dynamics model to study the electrophoretic behaviour of flexible polyelectrolyte chains. We first characterize the static properties of the model with respect to the chain length, the polyelectrolyte concentration, additional salt and the influence of an applied external field. Next we investigate the dynamic behaviour in the oligomer range and compare to data obtained by two different experimental methods, namely capillary electrophoresis and PFG-NMR. We find excellent agreement of experiments and simulations when hydrodynamic interactions are accounted for in the simulations. We then present novel estimators for the dynamical effective charge during free solution electrophoresis and compare them to static estimators. We find complete agreement between the static and the dynamic estimators. We further evaluate the scaling behaviour of the effective friction of the polyelectrolyte-counterion complex with the surrounding fluid. We identify a hydrodynamic screening length beyond which the friction during electrophoresis is linear depending on the chain length resulting in a constant mobility for long polyelectrolyte chains. Our results show a convincing agreement with experimental data and demonstrate that it is possible to model dynamic behaviour of polyelectrolytes using coarse grained models, provided they include the effects of hydrodynamical interactions.
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