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The pot experiments were conducted in the Department of Life Science and Bioinformatics of Assam University, Silchar. The aim of the present study was to investigate the effect of dual inoculation of Arbuscular mycorrhiza (AM) (Glomus fasciculatum) and Rhizobium on the chlorophyll, nitrogen and phosphorus contents of pigeon pea (Cajanus cajan L.). The results revealed an overall increase in chlorophyll, nitrogen and phosphorus contents in the inoculated plants than uninoculated ones. Maximum chlorophyll contents were recorded in the plants dually inoculated with Glomus fasciculatum and Rhizobium. The combined application of Glomus fasciculatum and Rhizobium also remarkably increased the nitrogen and phosphorus contents of pigeon pea. The dual inoculation with microsymbionts revealed synergistic effect. The results suggest that dual inoculation of Glomus fasciculatum and Rhizobium have the potential to enhance the chlorophyll, nitrogen and phosphorus contents of pigeon pea.