oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 4 )

2018 ( 48 )

2017 ( 43 )

2016 ( 47 )

Custom range...

Search Results: 1 - 10 of 1916 matches for " Suppression subtractive hybridization (SSH) "
All listed articles are free for downloading (OA Articles)
Page 1 /1916
Display every page Item
Chloroplast DNA Copy Number May Link to Sex Determination in Leucadendron (Proteaceae)
MADE PHARMAWATI,GUIJUN YAN,PATRICK FINNEGAN
HAYATI Journal of Biosciences , 2009,
Abstract: Leucadendron (Proteaceae) is a South African genus, the flowers of which have become a popular item in the Australian cut-flower industry. All species are dioecious. In general the female flowers are the more desirable as cut flowers. The availability of a molecular marker linked to sex determination is therefore needed both to maximize the efficiency of breeding programs and to supply markets with flowers from the preferred sex. The polymerase chain reaction-based method of suppression subtractive hybridization (SSH) combined with mirror orientation selection (MOS) were applied in an attempt to identify genome differences between male and female plants of Leucadendron discolor. Screening of 416 clones from a male-subtracted genomic DNA library and 282 clones from a female-subtracted library identified 13 candidates for male-specific genomic fragments. Sequence analyses of the 13 candidate DNA fragments showed that they were fragments of the chloroplast DNA, raising the possibility that chloroplast DNA copy number is linked to sex determination in Leucadendron.
Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening
Jin Zhou,Zhihua Liu,Xiuqin Wang,Fang Ding,Chuannong Zhou,Min Wu
Chinese Science Bulletin , 2001, DOI: 10.1007/BF02900601
Abstract: To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression subtractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such asliporcotin I,cystalin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with PCR-based doublestrand cDNA synthesis and high throughput reverse Northern screening is an efficient way to isolate differentially expressed genes from microgram of total RNA.
Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array
Junkui Ai,Zhiwen Zhang,Dianqi Xin,Hongjian Zhu,Quanjian Yan,Zhongcheng Xin,Yanqun Na,Yinglu Guo
Science China Life Sciences , 2004, DOI: 10.1360/03yc0043
Abstract: To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon membrane and the over-expressed genes were then screened by hybridizing the filter with radioactively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank database. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular endothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohistochemical and Western blot analysis for PTTG1 and ZNRD1 revealed increased protein level in RCC. The over-expressed genes in RCC are the potential molecular targets for diagnosis and therapy and it is very important to understand the molecular mechanism of RCC through the profile of over-expressed genes.
Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array
Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridi-zation and cDNA library array

AI Junkui,ZHANG Zhiwen,XIN Dianqi,ZHU Hongjian,YAN Quanjian,XIN Zhongcheng,NA Yanqun,GUO Yinglu,
AI Junkui
,ZHANG Zhiwen,XIN Dianqi,ZHU Hongjian,YAN Quanjian,XIN Zhongcheng,NA Yanqun & GUO Yinglu . Institute of Urology,Peking University &

中国科学C辑(英文版) , 2004,
Abstract: To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon mem-brane and the over-expressed genes were then screened by hybridizing the filter with radioac-tively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank da-tabase. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular en-dothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohis-tochemical and Western blot analysis for PTTG1 and ZNRD1 revealed increased protein level in RCC. The over-expressed genes in RCC are the potential molecular targets for diagnosis and therapy and it is very important to understand the molecular mechanism of RCC through the profile of over-expressed genes.
Identifying Differentially Expressed Genes in Pollen from Self-Incompatible “Wuzishatangju” and Self-Compatible “Shatangju” Mandarins
Hongxia Miao,Zixing Ye,Jaime A. Teixeira da Silva,Yonghua Qin,Guibing Hu
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14048538
Abstract: Self-incompatibility (SI) is one of the important factors that can result in seedless fruit in Citrus. However, the molecular mechanism of SI in Citrus is not yet clear. In this study, two suppression subtractive hybridization (SSH) libraries (forward, F and reverse, R) were constructed to isolate differentially expressed genes in pollen from “Wuzishatangju” (SI) and “Shatangju” (self-compatibility, SC) mandarins. Four hundred and sixty-eight differentially expressed cDNA clones from 2077 positive clones were sequenced and identified. Differentially expressed ESTs are possibly involved in the SI reaction of “Wuzishatangju” by regulating pollen development, kinase activity, ubiquitin pathway, pollen-pistil interaction, and calcium ion binding. Twenty five SI candidate genes were obtained, six of which displayed specific expression patterns in various organs and stages after self- and cross-pollination. The expression level of the F-box gene (H304) and S1 (F78) in the pollen of “Wuzishatangju” was 5-fold higher than that in “Shatangju” pollen. The F-box gene, S1, UBE2, UBE3, RNaseHII, and PCP were obviously up-regulated in pistils at 3 d after self-pollination of “Wuzishatangju”, approximately 3-, 2-, 10-, 5-, 5-, and 2-fold higher, respectively than that at the same stage after cross-pollination of “Wuzishatangju” × “Shatangju” pistils. The potential involvement of these genes in the pollen SI reaction of “Wuzishatangju” is discussed.
Molecular insights into how a deficiency of amylose affects carbon allocation – carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant
Ming-Zhou Zhang, Jie-Hong Fang, Xia Yan, Jun Liu, Jin-Song Bao, Gunnel Fransson, Roger Andersson, Christer Jansson, Per ?man, Chuanxin Sun
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-230
Abstract: Carbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin.Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds.Cereal crops are of critical importance in agriculture. The top three cereals in global production (2009) are maize, wheat, and rice, with 819, 686 and 685 M tonnes, respectively ( http://faostat.fao.org webcite). Cereal crops constitute our largest primary food source and are also highly used in food and non-food industrial applications. Contributing factors to the importance of cereals are that they can be bred to be very high yielding, that cereal grains lend themselves to long-term storage, and that the grain can accumulate different types of carbohydrates and lipids. Major carbohydrates in
Construction of a subtractive cDNA library from the internal organs of Trionyx sinensis experimentally infected by Aeromonas hydrophila
嗜水气单胞菌感染的中华鳖主要器官差减cDNA文库的构建

ZHOU Xiu-Xia,HUANG Rong,GUO Qiong-Lin,
周秀霞
,黄容,郭琼林

水生生物学报 , 2007,
Abstract: 以致病性嗜水气单胞菌(Aeromonas hydrophila)人工感染的中华鳖(Trionyx sinensis)肝、脾、肾组织为材料,应用抑制性差减杂交(SSH)技术,构建了嗜水气单胞菌感染组织的差减cDNA文库。以中华鳖管家基因-βactin作为差减指标检测该文库差减效率达210倍,表明感染细菌后某些差异表达基因得到了相应倍数的富集。将获得的cDNA片段连接到pMD18-T载体并转化大肠杆菌DH5α感受态细胞。PCR阳性检测显示差减片段在150—800bp之间。该差减cDNA文库的构建为快速分离和鉴定中华鳖与细菌感染相关的免疫基因及从分子水平探讨中华鳖的病理和抗感染免疫机制奠定了基础。
Construction and analysis of suppression subtractive library of Festuca arundinacea to low temperature stress
低温胁迫下高羊茅抑制消减文库的构建与分析

HUANG Jin-Wen,LUO Juan,CHEN Dong-Mei,ZHENG Hong-Yan,LIN Wen-Xiong,
黄锦文
,骆 娟,陈冬梅,郑红艳,林文雄

中国生态农业学报 , 2009,
Abstract: 采用抑制消减杂交(SSH)技术构建了高羊茅"猎狗5号"低温下的正向差减cDNA文库,并从基因表达水平研究了低温诱导对高羊茅抗寒性的影响.通过Reversenorthern杂交法筛选,挑选出36个低温处理下差异表达基因,并对这些基因进行测序.将测序结果在基因组数据库(NCBI)中进行检索比对,最后获得34个差异表达的基因,其中33个基因功能已知,主要与植物信号转导、光合作用、糖代谢、物质运输以及抗逆性相关.
以短暂暴露于邻苯二甲酸二丙酯(Dipropyl Phthalate)之淡水多齿新米虾(Neocaridina denticulata)建立生态毒理基因组生物标志物
Development of Ecotoxicogenomic Biomarkers on the Freshwater Shrimp (Neocaridina denticulate) Following Short-Term Exposure to Dipropyl Phthalate
 [PDF]

蔡伊茜, 宋宏红
International Journal of Ecology (IJE) , 2013, DOI: 10.12677/IJE.2013.24007
Abstract:
邻苯二甲酸酯类化合物(phthalate esters; PAEs)是广泛用于塑料制品之塑化剂和造成环境污染甚为可观的內分泌干擾物質。本研究以多齿新米虾(Neocaridina denticulata)暴露于次致死剂量(50 mg/L)之一種PAE——邻苯二甲酸二丙酯(dipropyl phthalate; DPrP)1天,利用抑制性扣减杂交法建立一套基因组指标,探讨其全面基因的表现变化。实验中共筛得71个独特的表达序列标签(expressed sequence tages; ESTs),包括23ESTs对应为已知功能基因及48ESTs为未知功能。根据生理功能,已知功能基因分别与9类相关,包括代谢、呼吸、防御、核糖体、染色体、转译、传讯、视觉与结构。虾子暴露于高浓度DPrP (50 mg/L)时,3个免疫及代谢相关的基因和3个未知功能基因,共6个基因的mRNA表现量明显下降。暴露于非致死剂量1.0 mg/L时,共8个基因受到影响,包括正调节的4个已知功能和1个未知功能基因,以及3未知功能基因被负调控。虾子暴露于更低剂量0.5 mg/L
CONSTRUCTION OF SUBSTRACTED cDNA LIBRARY OF LITOPENAEUS VANNAMEI INDUCED BY VIBRIO ALGINOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGS
溶藻弧菌诱导凡纳滨对虾消减文库的构建及其表达序列标签分析

CAI Xiao-Hui,LU Yi-Shan,WU Zao-He,JIAN Ji-Chang,WANG Bei,CAI Shuang-Hu,
蔡小辉
,鲁义善,吴灶和,简纪常,王蓓,蔡双虎

水生生物学报 , 2010,
Abstract: Vibrio alginolyticus is one of the main causative agents resulting in serious infectious diseases of Litopenaeus vannamei and other animals.Suppression subtractive hybridization(SSH) is a new and highly effective method for analysing differentially expressed or tissue-specific cDNA probes and libraries.It is applicable to diagnosis of disease,development,tissue specificity,or other differential expressing.Based on the user manual of Clontech PCR-SelectTM cDNA subtraction kit,a suppression subtraction cDNA l...
Page 1 /1916
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.