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Search Results: 1 - 10 of 225354 matches for " Sunil R. Dhaneshwar "
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Development and Validation of Stability Indicating RP-HPLC-PDA Method for Tenatoprazole and Its Application for Formulation Analysis and Dissolution Study  [PDF]
Sunil R. Dhaneshwar, Vaijanath N. Jagtap
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.22014
Abstract: In the present study, comprehensive stress testing of tenatoprazole was carried out according to ICH guide-line Q1A (R2). Tenatoprazole was subjected to stress conditions of hydrolysis, oxidation, photolysis and neutral decomposition. Extensive degradation was found to occur in acidic, neutral and oxidative conditions. Mild degradation was observed in basic conditions. The drug is relatively stable in the solid-state. Successful separation of drug from degradation products formed under stress conditions was achieved on a Kromasil C18 column (250 mm × 4.6 mm, 5.0 μ particle size) using methanol: THF: acetate buffer (68:12:20 v/v) pH adjusted to 6.0 with acetic acid as mobile phase, flow rate was 1.0 mL●min–1 and column was maintained at 45°C. Quantification and linearity was achieved at 307 nm over the concentration range of 0.5 - 160 μg●mL–1 for tenatoprazole. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness.
Development and validation of a stability-indicating lc method for the determination of tenofovir disoproxil fumarate in pharmaceutical formulation
Shweta Havele,Sunil R. Dhaneshwar
Songklanakarin Journal of Science and Technology , 2012,
Abstract: The present study describes the degradation of tenoforvir disoproxil fumarate (teno) under different prescribed stressconditions (hydrolysis, oxidation, dry and wet heat and photolysis) following the International Conference on Harmonization and application of a specific and selective stability-indicating reversed-phase high–performance liquid chromatography(HPLC) assay. Separation of drug and degradation products was successfully achieved on C18 analytical column usingmethanol: water (60:40, v/v) at a flow rate of 1.0 ml/min and detection at 260 nm, the mass balance was found to be close to100.4%. The developed HPLC method was validated with respect to linearity, accuracy, precision, robustness, and accuracy.
Application of a stability-indicating TLC method for the quantitative determination of Levocetirizine in pharmaceutical dosage forms
Vidhya K Bhusari,Sunil R Dhaneshwar
International Journal of Advances in Pharmaceutical Sciences , 2011, DOI: 10.5138/193
Abstract: A sensitive, selective, precise and stability-indicating thin layer chromatographic method of analysis for Levocetirizine both as a bulk drug and in formulation was developed and validated. The method employed TLC aluminium plates precoated with silica gel 60 F-254 as the stationary phase and the solvent system consisted of ethyl acetate: methanol: ammonia (9: 2.5: 1.5 v/v/v). This system was found to give compact spots for Levocetirizine (Rf value of 0.50 ± 0.02). Levocetirizine was subjected to acid and alkali hydrolysis, oxidation, photo and neutral degradation. The peak of the degradation product was well resolved from the pure drug and had significantly different Rf value. Densitometric analysis of Levocetirizine was carried out in the absorbance mode at 230 nm. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 200-1200 ng/spot. The mean values of the correlation coefficient, slope and intercept were 0.9904 ± 1.53, 2.8899 ± 0.998 and 802.6 ± 0.982 respectively. The method was validated for precision, robustness and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 100 and 200 ng/spot respectively. Statistical analysis showed that the method is repeatable and selective for the estimation of Levocetirizine. Since the method can separate the drug from its degradation products, it can be used to monitor stability. Keywords: Degradation; Levocetirizine; TLC; stability-indicating.
Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation
Vidhya K. Bhusari,Sunil R. Dhaneshwar
ISRN Analytical Chemistry , 2012, DOI: 10.5402/2012/609706
Abstract:
Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation
Vidhya K. Bhusari,Sunil R. Dhaneshwar
ISRN Analytical Chemistry , 2012, DOI: 10.5402/2012/609706
Abstract: This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Atenolol and Aspirin as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of n-butanol?:?water?:?acetic acid (8?:?2?:?0.2 v/v/v). Densitometric evaluation of the separated zones was performed at 235?nm. The two drugs were satisfactorily resolved with R F values 0 . 2 3 ± 0 . 0 2 and 0 . 7 2 ± 0 . 0 2 for Atenolol and Aspirin, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (100–600?ng/spot for Atenolol and Aspirin), precision (intraday % RSD was 0.48–1.03 and interday % RSD was 0.68–1.14 for Atenolol, and intraday % RSD was 0.61–1.03 and interday % RSD was 0.69–1.04 for Aspirin), accuracy ( 1 0 0 . 1 7 ± 0 . 3 2 for Atenolol and 9 9 . 7 3 ± 0 . 5 1 for Aspirin), and specificity in accordance with ICH guidelines. 1. Introduction Atenolol is chemically (RS)-2-{4-[2-hydroxy-3-(propan-2-ylamino)propoxy]phenyl } acetamide (Figure 1). Atenolol is a beta-adrenergic blocking agent that blocks the effects of adrenergic drugs. The important function of beta-adrenergic stimulation is to stimulate the heart to beat more rapidly. By blocking the stimulation of these nerves, Atenolol reduces the heart rate and is useful in treating abnormally rapid heart rhythms. Atenolol is also helpful in treating angina [1]. Figure 1: Structure of Atenolol. Aspirin chemically is 2-acetoxybenzoic acid (Figure 2). Aspirin reduces inflammation, analgesia, prevents clotting, and reduces fever. It is believed to decrease production of prostaglandins and thromboxanes. Aspirin’s ability to suppress the production of prostaglandins and thromboxanes is due to its irreversible inactivation of the cyclooxygenase (COX) enzyme. Cyclooxygenase is required for prostaglandin and thromboxane synthesis. Aspirin acts as an acetylating agent where an acetyl group is covalently attached to a serine residue in the active site of the COX enzyme [2]. Figure 2: Structure of Aspirin. Today TLC is rapidly becoming a routine analytical technique due to its advantages of low operating costs, high sample throughput, and the need for minimum sample preparation. The major advantage of TLC is that several samples can be run simultaneously using a small quantity of mobile phase unlike HPLC, thus reducing the analysis time and cost per analysis. Literature review reveals that methods have been reported for
Research: VALIDATED HPLC METHOD FOR SIMULTANEOUS QUANTITATION OF LEVOCETIRIZINE HYDROCHLORIDE AND NIMESULIDE IN BULK DRUG AND FORMULATION
Sunil R Dhaneshwar*,Janaki V Salunkhe,Vidhya K Bhusari
Pharmacie Globale : International Journal of Comprehensive Pharmacy , 2011,
Abstract: HPLC method has been described for simultaneous determination of Levocetirizine hydrochloride andNimesulide in formulation. This method is based on HPLC separation of the two drugs on the HiQ Sil C18 HS (250mm × 4.6 mm, 5.0 μ), Germany with isocratic conditions and simple mobile phase containing methanol: water(70: 30) at flow rate of 1 mL/min using UV detection at 239 nm. This method has been applied to formulationwithout interference of excipients of formulation. The linear regression analysis data for the calibration plotsshowed a good linear relationship over the concentration range of 1-6 μg/mL for Levocetirizine hydrochlorideand 4-24 μg/mL for Nimesulide respectively. The mean values of the correlation coefficient, slope and interceptwere 0.9990 ± 1.27, 34675 ± 1.18 and 8943.5 ± 1.82 for Levocetirizine hydrochloride and 0.9992 ± 0.78, 40863 ±1.21 and 5209.2 ± 1.09 for Nimesulide respectively. The method was validated for precision, robustness andrecovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 0.25 μg/mL and 0.5 μg/mL forLevocetirizine hydrochloride and 2 μg/mL and 3 μg/mL for Nimesulide, respectively. Statistical analysis showedthat the method is repeatable and selective for the estimation of Levocetirizine hydrochloride and Nimesulide.
Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops
S. S. Havele,S. R. Dhaneshwar
ISRN Analytical Chemistry , 2012, DOI: 10.5402/2012/414075
Abstract:
Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops
S. S. Havele,S. R. Dhaneshwar
ISRN Analytical Chemistry , 2012, DOI: 10.5402/2012/414075
Abstract: The present study describes the degradation of dorzolamide HCl under different International Conference on Harmonization prescribed stress conditions (hydrolysis, oxidation, thermolysis, and photolysis) and application of a specific and selective stability-indicating chromatography assay. HPTLC was done on silica Gel 60 F254 TLC plate using chloroform: methanol in the ratio of 9.0?:?1.0 (v/v) as mobile phase and detection at 253?nm. 1. Introduction Dorzolamide hydrochloride (DORZO), (4S,6S)-4-ethylamino-5,6-dihydro-6-methyl-4H-thieno(2,3-b)thiopyran-2-sulfonamide 7,7-dioxide monohydrochloride (Figure 1), is a carbonic anhydrase inhibitor used in eye drops to treat increased pressure in the eye caused by open-angle glaucoma and to treat a condition called hypertension of the eye [1]. Figure 1: Chemical structure of dorzolamide hydrochloride. The literature review reveals that several methods have been reported for the estimation of DORZO in biological fluids [2–5], and there are some methods reported by HPLC [6–9], spectroscopy [10, 11], and capillary electrophoresis [12], and there are some methods reported for estimation of DORZO in pharmaceutical formulation [13–15]. According to current good manufacturing practices, all drugs must be tested with a stability-indicating assay method before release. So far, to our present knowledge, no stability-indicating HPTLC assay method for the determination of DORZO is available in the literature. Now a days, HPTLC is rapidly becoming a routine analytical technique due to its advantages of low operating costs, need for minimum sample preparation, and high sample throughput. The major advantage of HPTLC is that several samples can be run simultaneously using a small quantity of mobile phase unlike HPLC—thus reducing the analysis time and cost per analysis. It was felt necessary to develop stability-indicating HPTLC method for the determination of DORZO as bulk drug and pharmaceutical dosage form and separate the drugs from the degradation products under the ICH-suggested conditions. Therefore, the aim of the present study was to develop and validate stability-indicating HPTLC assay methods for DORZO as bulk drug and in pharmaceutical dosage form as per International Conference on Harmonisation guidelines [16–19]. 2. Experimental 2.1. Drug and Reagents Pharmaceutical grade DORZO working standard was obtained as generous gift from FDC Ltd., Mumbai, India. The eye drop sample (Ocudor eye drops, manufactured by Lumina (FDC Limited)) was obtained commercially. The DORZO was labeled (per 1?mL) as 22.26?mg. All chemicals
CFD ANALYSIS OF FRANCIS TURBINE
Ravindra R. Navthar,Joshi Tejas,Dhaneshwar Saurabh,Domale Nitish
International Journal of Engineering Science and Technology , 2012,
Abstract: Hydroelectric power has become the most promising source in power sector to sustain the growth of any nation. In any hydroelectric power plant, hydraulic turbine plays a vital role which affects the overall performance of the plant and if utilized at suboptimal level, may lead to the loss of useful head. So, it becomes vital to predict the behavior of hydro-turbine under actual working conditions. Experimental approach of predicting the performance of hydro turbine is costly and time consuming compared to CFD approach. CFD has emerged out as a powerful tool for predicting the performance of mechanical bodies subjected to dynamic flow conditions. There are ample of evidences where analysts at various levels have taken the advantage of this tool to solve so many problems related to performance analysis. Along with it, simulation technique is key feature. The aim of this paper is to analyze the turbine for mechanical failure along with predicting its performance under actual operating condition by using CFD which is generally constrained by using a prototype. With the technique of simulation we can not only get the values of performance parameters but also the visualization of concentration and distribution of various parameters like pressure, velocity, stress at various locations where we may get versed with critical zones where the failure is supposed to occur.
Spontaneous Retropharyngeal Haematoma in a One-Year-Old Child: A Case Report  [PDF]
R. K. Mundra, Richi Sinha, Sunil Bajoliya
Case Reports in Clinical Medicine (CRCM) , 2014, DOI: 10.4236/crcm.2014.35062
Abstract:

Spontaneous retropharyngeal haematoma is an unusual condition and difficult to diagnosis early especially in infants. It has multiple aetiological factors and can present to a number of specialists including the otolaryngologist. We describe an unusual case of a spontaneous huge retropharyngeal haematoma in 1-year-old male child which demonstrates the dramatic presentation and emphasizes the need for immediate surgical approach.

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