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Search Results: 1 - 10 of 4628 matches for " Suman Preet; Nath Mishra "
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Genetic transformation studies and scale up of hairy root culture of Glycyrrhiza glabra in bioreactor
Mehrotra,Shakti; Kumar Kukreja,Arun; Singh Khanuja,Suman Preet; Nath Mishra,Bhartendu;
Electronic Journal of Biotechnology , 2008,
Abstract: the study was undertaken to induce hairy roots in glycyrrhiza glabra in leaf explants and to optimize the nutritional requirement for its growth kinetics at shake flask and bioreactor level. pathogenecity of agrobacterium depends upon transformation ability of strain and age, type, and physiological state of explants. agrobacterium rhizogenes strain k599 was used to infect leaf explants of g. glabra. explants of different age groups were obtained from 2 to 5 weeks old in vitro grown cultures. bacterial strain k599 could induce hairy roots in 3 and 4 weeks old leaf explants cultured on b5, ms, nb and wp basal semi-solid medium. leaf explants of 2 and 5 weeks old culture were not responsive to bacterial infection in terms of hairy root induction. maximum transformation frequency (tf) of tested bacterial strain was 47% obtained in 3 weeks old explants after 25 days of incubation on ms basal semi solid medium. nb and b5 both media composition showed 20% of transformation frequency after 28 and 38 days respectively. wp medium did not support induction of roots in cultured leaf explants infected with a. rhizogenes strain k599even after 50 days of incubation. further, when all the four media combinations were tested for root growth it was found that though wp was not responsive for hairy root induction, yet all four basal media supported hairy root growth and a gradual increase in fresh weight biomass was observed with an increase in culture duration. however amongst all, the nb medium composition supported best growth of hairy roots followed by ms, b5 and wp media. about 20 times increase in root biomass on fresh weight basis was recorded after 45days of culture in nb medium. initial inoculum of roots (0.18 g. f.wt./ flask) containing 50 ml of liquid culture medium produced 3.59 g (f. wt.) biomass. a fast growing hairy root clone g6 was grown in a 5 l capacity mechanically agitated bioreactor provided with a nylon mesh septum. after 30 days of sterile run, 310 g of root b
Genetic Variability in Germplasm Accessions of Capsicum annuum L  [PDF]
Shrilekha Misra, Raj Kishori Lal, Mahendra Pandurang Darokar, Suman Preet Singh Khanuja
American Journal of Plant Sciences (AJPS) , 2011, DOI: 10.4236/ajps.2011.25074
Abstract: Capsicum annuum is the most widely cultivated species of peppers (chilies) in the world. For culinary purposes, its fruits are used for pungency (capsaicin) and also color (capsanthin). Capsaicin is also used for medicinal purposes particularly in anti-inflammatory formulations. Genetic divergence among 38 accessions collected from diverse locations in India (28 from Uttar Pradesh, 5 from Assam, 3 from Maharashtra and 2 from Uttaranchal), was estimated from the data pooled over 3 consecutive years for 15 morphological, growth and chemotypic characters that included days to first and second flowering, fruit onset, plant height, primary, secondary and tertiary branches, leaf surface area, fruit length and diameter, fruit surface area, fresh and dry fruit weight, capsaicin and capsanthin content. Based on this characterization the plants could be grouped into 7 clusters wherein substantial diversity among accessions was indicated by the wide range of D2 values (752.901 - 1918683.00). Accessions with distinct identity were marked, which are likely to be quite suitable for breeding through hybridization combining desirable traits. The accessions labeled number 38, 27, 26, 14 and 24 to high capsaicin content (%); 35, 23, 3, 16, 29 and 11 for high capsanthin content (%) and 26 and 27 for dual purpose had characteristics desirable. Above accessions could be utilized in hybridization programme for C. annuum crop improvement.
Cost-effective Approaches for in vitro mass Propagation of Rauwolfia serpentina Benth. Ex Kurz.
Manoj Kumar Goel,Arun Kumar Kukreja,Suman Preet Singh Khanuja
Asian Journal of Plant Sciences , 2007,
Abstract: The present study describes a highly efficient and a very cost effective micropropagation protocol for exsitu conservation of an important medicinal plant, Rauwolfia serpentina using glass beads as support matrix in liquid culture medium and as a potential alternative to agar and market grade sugar as carbon source. Glass beads can replace the conventionally used gelling agent agar without compromising the quality of the in vitro regenerated shoots/plantlets of R. serpentina. This practice could be helpful in achieving more than 95% reduction in cost media for the production of single regenerated shoot, moreover, the glass beads can be reused for indefinite time after proper acid wash.
CSF tau and amyloid β42 levels in Alzheimer’s disease—A meta-analysis  [PDF]
Rachna Agarwal, Neelam Chhillar, Vijay Nath Mishra, Chandra Bhushan Tripathi
Advances in Alzheimer's Disease (AAD) , 2012, DOI: 10.4236/aad.2012.13005
Abstract: Alzheimer’s disease International (ADI) estimates that there are currently 30 million people with dementia in the world. The main objective was to perform meta-analysis of studies of CSF tau and Amyloid β42 (Aβ42) levels in Alzheimer’s disease (AD) patients and controls. In the present study MEDLINE was reviewed from 1995 to 2009, supplemented by citation analysis from retrieved articles to select case control studies. Descriptive statistics showed that median effect size (raw mean difference) of CSF tau and Aβ42 levels were 301 pg/ml (Range: 22 to 614 pg/ml) and –352 pg/ml (Range: –969 to 203 pg/ml) respectively. The pooled effect size CSF tau and Aβ42 was 289.14 pg/ml (95% CI 253.278 to 325.013 pg/ml) and –329.02 pg/ml (95% CI –387.740 to –270.445 pg/ml) respectively. Heterogeneity in effect size of selected studies was present for both parameters (CSF tau: Q statistics = 1816.596, DF = 40, P = 0.000 and CSF Aβ42: Q-statistics = 1259.358, DF = 24, p < 0.001). Based on the findings of meta-analysis in the present study, CSF tau and Aβ42 levels in AD and controls may be considered as potential biomarker along with the clinical phenotype to perform them during high quality diagnostic testing in dementia.
Village Forums or Development Councils: People’s participation in decision-making in rural West Bengal, India
Raghabendra Chattopadhyay,Bhaskar Chakrabarti,Suman Nath
Commonwealth Journal of Local Governance , 2010, DOI: 10.5130/cjlg.v0i5.1490
Abstract: The policy shift towards decentralisation promises important social change in rural India, providing as it does a three-tier system of local self-governments, the Panchayats: at the village level, the district level, and an intermediate level between the two, called the Block Panchayat. There is evidence of far-reaching social change in rural West Bengal, a state in eastern India, after the Left Front government came into power, particularly because of revitalisation of the three-tier Panchayat system. The initial years of Left Front rule saw the village poor enthusiastically attending Panchayat meetings and taking part in decision-making at the village council, the Gram Sabha, the general body of villagers of voting age covering 10-12 villages, and the Gram Sansad, the forum of local democracy at the ward level. However, today, relatively few people in the villages are taking part in government-sponsored initiatives. Panchayat meetings are scarcely attended and almost always exclude certain classes and members of the community. In order to combat the problem, the Government of West Bengal has recently tried to further devolve the power and responsibilities of local government and has established Gram Unnayan Samiti (GUSs) or Village Development Councils, consisting of political members from both elected and the opposition parties and certain nominated members. The GUSs are supposed to bring in more participation at the grassroots level. In this paper, we study the formal policies regarding decentralisation and people’s participation in West Bengal, and analyse the dynamics of political processes regarding decision-making at operational level after the introduction of GUS. We have analysed audio recordings of meetings of the Gram Sabhas and the dynamics of the newly formed GUSs to uncover the actual rate of people’s participation, actual meeting procedures and reasons behind people’s reluctance to participate. We argue that solutions lie in having a strong third-tier in order to address issues of lack of transparency and accountability in decision-making, and make recommendations as to how that might be achieved.
Local Governments in Rural West Bengal, India and their Coordination with Line Departments
Bhaskar Chakrabarti,Raghabendra Chattopadhyay,Suman Nath
Commonwealth Journal of Local Governance , 2011, DOI: 10.5130/cjlg.v0i8/9.2411
Abstract: In India, the 73rd constitutional amendment of 1992 decentralises agriculture, irrigation, health, education along with 23 other items to the Panchayats, the village level self-government body. It is envisaged that the three-tier Panchayat system at the District, Block and the Village level would coordinate with different ‘line departments’ of the government for planning various schemes and their implementation. In West Bengal, a state in eastern India, where the Panchayats were revitalised before the constitutional amendment, the initial years were marked by strong coordination between the Panchayats and other departments, especially land and agriculture, making West Bengal a ‘model’ case for the Panchayats. However, where service delivery through the Panchayats has been criticised in recent years, the disjuncture between Panchayats and the line departments is a cause for alarm. In this paper, we search for the causes behind the low level of coordination between government departments and the Panchayat at each tier. We analyse the complex process of organisational coordination that characterises decentralisation, and show how decision making in local governments is nested within various levels of hierarchy. The study focuses on the formal structures of coordination and control with regard to decision-making between the Panchayats and the line departments. We show how these processes work out in practice. These involve lack of role definition, problems of accountability, and politics over access to resources and relations of power within, as well as outside, the Panchayat.
Implementation of AWG as A MUX / DMUX at the WDM PON System
Prem Nath Suman1 , Dharmendra Singh
International Journal of Engineering Research , 2014,
Abstract: PON (PASSIVE OPTICAL NETWORK) is going to be the one of the basic requirement of the future demands of the tele-communication system. The implementation of the WDM-PON along with the AWG is done carefully in the in the system. The AWG is used in both of the transmitter and the receiver side, as a multiplexer and demultiplexer. Our set-up model consists of the both up-stream and the down-stream data transmission. In the down- stream AWG multiplex the signal and behaves like a multiplexer at the OLT side and demultiplex the signal at the ONU side. And similar is the case with the up-stream communication system. We developed analytical model for studying the impact of the transmission impartment as well as the AWG characteristics on the BER performance of the WDMPON by incorporating the novel spectral-to-spectral domain transformation technique. The very main concept of the proposed model is the AWG . The power being captured by the output port of the AWG is not only based on the Gaussian focal field pattern but also to the power spill over the adjacent ports. All the results are strongly supported with the help of the Q factor and the eye diagram which are being executed from the BER analyser at the receiver part of the set up model. At the analysis section the plotting of the different parameter is also demonstrated. As a software Optiwave 7 software is used for the complete analysis purpos
A graph-based clustering method applied to protein sequences
Pooja Mishra,Paras Nath Pandey
Bioinformation , 2011,
Abstract: The number of amino acid sequences is increasing very rapidly in the protein databases like Swiss-Prot, Uniprot, PIR and others, but the structure of only some amino acid sequences are found in the Protein Data Bank. Thus, an important problem in genomics is automatically clustering homologous protein sequences when only sequence information is available. Here, we use graph theoretic techniques for clustering amino acid sequences. A similarity graph is defined and clusters in that graph correspond to connected subgraphs. Cluster analysis seeks grouping of amino acid sequences into subsets based on distance or similarity score between pairs of sequences. Our goal is to find disjoint subsets, called clusters, such that two criteria are satisfied: homogeneity: sequences in the same cluster are highly similar to each other; and separation: sequences in different clusters have low similarity to each other. We tested our method on several subsets of SCOP (Structural Classification of proteins) database, a gold standard for protein structure classification. The results show that for a given set of proteins the number of clusters we obtained is close to the superfamilies in that set; there are fewer singeltons; and the method correctly groups most remote homologs.
Transverse energy and charged particle production in heavy-ion collisions: From RHIC to LHC
Raghunath Sahoo,Aditya Nath Mishra
Physics , 2013, DOI: 10.1142/S0218301314500244
Abstract: We study the charged particle and transverse energy production mechanism from AGS, SPS, RHIC to LHC energies in the framework of nucleon and quark participants. At RHIC and LHC energies, the number of nucleons-normalized charged particle and transverse energy density in pseudorapidity, which shows a monotonic rise with centrality, turns out to be an almost centrality independent scaling behaviour when normalized to the number of participant quarks. A universal function which is a combination of logarithmic and power-law, describes well the charged particle and transverse energy production both at nucleon and quark participant level for the whole range of collision energies. Energy dependent production mechanisms are discussed both for nucleonic and partonic level. Predictions are made for the pseudorapidity densities of transverse energy, charged particle multiplicity and their ratio (the barometric observable, $\frac{dE_{\rm{T}}/d\eta}{dN_{\rm{ch}}/d\eta} ~\equiv \frac{E_{\rm{T}}}{N_{\rm{ch}}}$) at mid-rapidity for Pb+Pb collisions at $\sqrt{s_{\rm{NN}}}=5.5$ TeV. A comparison with models based on gluon saturation and statistical hadron gas is made for the energy dependence of $\frac{E_{\rm{T}}}{N_{\rm{ch}}}$.
A Methodology for Selection of Optimum Power Rating of Propulsion Motor of Three Wheeled Electric Vehicle on Indian Drive Cycle (IDC)
Prasun Mishra,Suman Saha,H. P. Ikkurti
International Journal on Theoretical and Applied Research in Mechanical Engineering , 2013,
Abstract: Optimum power rating of propulsion motor is an important issue for designing efficient drive train for a three wheeled battery operated electric vehicle application on standard drive cycle like Indian Drive Cycle of Indian urban and suburban areas. This paper deals with proper estimation of power rating of electric motor by root means square technique while considering the limitations of overloading and under loading operation of motor used in electric vehicle along with some specified design parameters, aiming towards better performance than that of a conventional IC engine driven vehicle. Dynamics of three wheeled vehicle is simulated on MATLAB/Simulink environment under a sequence of road grade angle variation in Indian Drive cycle (IDC), which is formulated by Automotive Research Association of India, Pune. Using simulated vehicle dynamics, power rating of the motor is estimated so that it can be operated efficiently and safely throughout the entire Indian drive Cycle.
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