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β-Glucan production of Saccharomyces cerevisiae in medium with different nitrogen sources in air-lift fermentor
Biodiversitas , 2007,
Abstract: β-Glucan is one of the most abundant polysaccharides in yeast Saccharomyces cerevisiae cell wall. The aim of this research is to explore an alternative nitrogen sources for β-glucan production. S. cerevisiae were grown in fermentation medium with different nitrogen sources. Peptone 2%, glutamic acid 0,5%, urea 0,2%, and diammonium hydrogen phosphate (DAHP) 0,02% were used for nitrogen source in the medium. A two liter air-lift fermentor was used in the fermentation process for 84 hours (T = 300C, pH 7, and 1.5 vvm for the aeration). During the fermentation, optical density, extraction of β-glucan, glucose and protein in hydrolisate cultured were determined. β-glucan production level is similar with the growth rate of yeast and followed by decreasing glucose and protein content in hydrolysis cultured. The highest and lowest β-glucan content were obtained from peptone (933.33 mg/L) and glutamic acid (633.33 mg/L) as a nitrogen source in cells cultured after fermentation completed respectively. Yeast cells cultured with urea and DAHP as a nitrogen source give the same content of β-glucan about 733.33 mg/L. β-glucan concentration produced in medium with urea was a higher than that produced using glutamic acid and DAHP as a nitrogen source. The result indicated that urea can be used as an alternative nitrogen source for the production of β-glucan. Urea is easily available and cheaper than peptone, glutamic acid and DAHP.
Production of Beta-1,3 Glucan from Agrobacterium and Its Wound Healing Activity on White Rat
Kusmiati,Amarila Malik,Sukma Nuswantara,Syafrida Siregar
Makara Seri Sains , 2006,
Abstract: The objective of this study was to determine the activity of beta-1,3 glucan product extracted from local Agrobacterium sp Bro 1.2.1, both wild-type and mutant-type, on opened-wound healing process. Beta-1,3 glucan product was extracted by precipitation, and the purification was carried out by column chromatography as KCl gradient fractions. In this study, white Sprague Dawley rats were employed, and have been treated for opened-wound condition. Seven groups were performed in this experiment, i.e. the negative control, the positive control employing povidone iodine, the two groups of two commercial beta-1,3 glucan with 0,02 mg/4 cm2 each, and the last three groups of beta-1,3 glucan as the test group with 0,02 mg/4 cm2, 0,10 mg/4 cm2 and 0,50 mg/4 cm2, respectively. The result showed significant differences of wound-healing activity performing statistical analysis of the least significance between the negative control, the positive control, as well as the highest dose of the test group of beta-1,3 glucan, at the dose of 0,5 mg/4 cm2 (p<0.05).
Influence of Uracil in Fermentation Media on β-Glucan Production by Agrobacterium Radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1
Kusmiati,Salmah Muhamad,Sukma Nuswantara,Swasono R.Tamat
Makara Seri Sains , 2007,
Abstract: Optimum β-glucan production can be achieved by an optimum condition in the fermentation media. Uracil, as a precursor of UDP-glucose, may act as a glucose donor in the formation of polysaccharides such as β-glucan. It is expected that addition of certain quantity of uracil into the fermentation media in a suitable growth phase of Agrobacterium radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1, will significantly increase the β-glucan production. In this investigation, 0.025%; 0.05% or 0.1% of uracil were added into the fermentation media during the logarithmic phase (24 hour) or stationary phase (46 hour) of growth. The β-glucan product was evaluated from the β-glucan (crude) dry-weight and from the β-glucan content. Beta-glucan content was determined as glucose by the Hisamatsu-AOAC and HPLC methods. The highest β-glucan (crude) dry-weight produced by the A. 1.5 was in a medium containg 0.025% uracil (24 hour), whilst by the A. Bro 1.2.1 was in a medium containg 0.1% uracil (46 hour), both higher than control. The highest β-glucan content produced by the A. 1.5 (27.03%) was in a medium containg 0.025% uracil (46 hour), while control produced only 23.28%. The highest β-glucan content produced by the Bro 1.2.1 (29.34%) was in a medium containg 0.025% uracil (24 hour), while control produced only 28.75%. Two-way anova analysis showed that there were no significant influence difference (α = 0,05) from various concentration of uracil in either growth phases, to the yield of β-glucan (crude) dry-weight nor to the β-glucan equivalent glucose content.
Kartika Nuswantara
Jurnal Sosial Humaniora , 2013, DOI: 10.12962/j24433527.v6i1.613
Abstract: Being a teacher could be committed by anyone who has the passion in teaching, so that any situations in reality would not hamper him from the struggle to be a good teacher. Classroom is the laboratory for the teachers for their personal development. Students from whom the teachers can learn are their other assets. If an unfortunate system keeps going without any authority from the side of the teacher to change, being a quality teacher should still become teachers’ efforts to realize.
Development of Sequence-Based Microsatellite Marker for Phalaenopsis Orchid
HAYATI Journal of Biosciences , 2011,
Abstract: Phalaenopsis is one of the most interesting genera of orchids due to the members are often used as parents to produce hybrids. The establishment and development of highly reliable and discriminatory methods for identifying species and cultivars has become increasingly more important to plant breeders and members of the nursery industry. The aim of this research was to develop sequence-based microsatellite (eSSR) markers for the Phalaenopsis orchid designed from the sequence of GenBank NCBI. Seventeen primers were designed and thirteen primers pairs could amplify the DNA giving the expected PCR product with polymorphism. A total of 51 alleles, with an average of 3 alleles per locus and polymorphism information content (PIC) values at 0.674, were detected at the 16 SSR loci. Therefore, these markers could be used for identification of the Phalaenopsis orchid used in this study. Genetic similarity and principle coordinate analysis identified five major groups of Phalaenopsis sp. the first group consisted of P. amabilis, P. fuscata, P. javanica, and P. zebrine. The second group consisted of P. amabilis, P. amboinensis, P. bellina, P. floresens, and P. mannii. The third group consisted of P. bellina, P. cornucervi, P. cornucervi, P. violaceae sumatra, P. modesta. The forth group consisted of P. cornucervi and P. lueddemanniana, and the fifth group was P. amboinensis.
Kartika Nuswantara, Salsabela Putri Aghnadiin
Jurnal Sosial Humaniora , 2016, DOI: 10.12962/j24433527.v9i1.1276
Abstract: Paper ini mencoba menguraikan ungkapan-ungkapan bahasa Jepang yang umum diajarkan pengajar bahasa Jepang pada pembelajar yang berbicara bahasa Indonesia. Teori analisis kontrastif digunakan untuk memperoleh persamaan sekaligus perbedaan antara ungkapan pada bahasa Jepang dan bahasa Indonesia. Sumber acuan data diambil dari buku ajar Marugoto A1 jilid katsudou. Diperoleh 120 data ungkapan, dan diantaranya yaitu, 97 ungkapan yang mirip, 21 ungkapan yang memiliki perbedaan, dan 2 ungkapan yang tidak ada pembandingnya dalam bahasa Indonesia. Dari hasil ini, didapat bahwa perlu perhatian lebih pada ungkapan yang memiliki perbedaan kemiripan, yaitu ungkapan ajakan/undangan dan menanggapi ajakan/undangan, menawarkan, dan bertamu, serta ungkapan yang tidak ada ungkapan pembanding dari Bahasa Indonesia seperti ungkapan “itadakimasu”-“gochisoosama”.
Eka Dian Savitri, Kartika Nuswantara, Siti Zahrok
Jurnal Sosial Humaniora , 2016, DOI: 10.12962/j24433527.v9i2.1624
Abstract: This is a descriptive qualitative study that aims to describe Indonesian economists language attitude, specifically on the way they label the products.By means of behaviorist approach, the study raises an interpretation of the economists throughot observation. In order to come to the answer of the problem, data employed in this present study is the secondary data derived from other researchers. Then, the analysis of the data is employed by reviewing the labeling process in three ways, namely reasons for labeling, characteristics of the language attitude, and the Act 24 year 2009. It is figured out that there are 29 products labeled using foreign words or phrase, and the rest 11 products useIndonesia language. The phenomenon shows that labeling in foreign languages would enhance the positive image of the product. On the other hand, positive attitude is shown by the economists through their loyalty using bahasa Indonesia for labelling. Final finding is to show that the attitude displays the conterproductive to the Act 24 of 2009
Studies on phytosterol content of Parkia roxburgii G. Don.
Biodiversitas , 2006,
Abstract: Kedawung (Parkia roxburgii G. Don.) is one of plants that originally could be found in almost all parts of Java Island. Due to lack of attention, at present situation this plant could be categorized as an endangered species. This plant species distributed widely in Africa. In several African countries, this plant has an important position either as food resource or alternative medicine. In Indonesia, traditionally kedawung tree often use as medicine, especially as part of “jamu gendong” formulation. This work was focused on the study of phytosterol content and it distribution. The research result show that almost all parts of kedawung tree have a significant content of phytosterol, which is dominated by beta-sitosterol. The highest beta-sitosterol content was founded in the stem of leaf (35.24% (w/w) and pod (29.67% (w/w).
Perception and Attitudes toward Terrorism in a Muslim Majority Country
Kamarulnizam Abdullah,Rizal Sukma,Ma’ruf Jamhari,Mazilan Musa
Asian Social Science , 2012, DOI: 10.5539/ass.v8n4p77
Abstract: The purpose of this research article is to examine and to ascertain whether general public in a Muslim majority country do support organized violence and terrorism. The discussion focuses on Malaysia as a case study. The study adopted a quantitative approach with questionnaires being used as the instrument for data collection. The data were analyzed using Statistical Package for Social Sciences (SPSS). The results show that the Malaysian public do not support terrorism. The findings also found that ethnicity and religiosity have important bearings toward political violence and terrorism. Malay-Muslims, for instance, tend to exhibit some inclinations toward aggressive attitude compared to that of non-Malay Muslims. Furthermore, the study also found that gender, race, religion and occupation do play a part in determining perception on acts of terror.
Association of beta3-adrenergic receptor (ADRB3) Trp64Arg gene polymorphism with obesity and metabolic syndrome in the Balinese: a pilot study
Safarina G Malik, Made R Saraswati, Ketut Suastika, Hidayat Trimarsanto, Sukma Oktavianthi, Herawati Sudoyo
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-167
Abstract: A total of 528 Balinese (urban 282, rural 246) were recruited. Body mass index (BMI) and waist circumference (WC) were determined; high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), systolic and diastolic blood pressure (SBP and DBP), and fasting plasma glucose (FPG) were measured using standard procedures. BMI and WC classifications were based on WHO classifications for Asian. Metabolic syndrome (MetS) was defined as described in the Joint Interim Statement. Chi-square test was employed to test the association between the ADRB3 Trp64Arg genotype and disease traits.Urban have higher BMI (p = 2.8 × 10-13), WC ( p < 2.2 × 10-16), TG (p = 0.0028), DBP (p = 1.8 × 10-5), and lower HDL-C (p = 0.0376) when compared to rural. Abdominal obesity and MetS prevalence were significantly higher in urban as compared to rural (both p < 0.001). The Arg64 allele frequency was similar between urban (0.06) and rural (0.05). The Arg64 rural female carriers have higher BMI and WC as compared to their Trp64 counterparts (p = 0.041 for BMI and p = 0.012 for WC), and consequently higher abdominal obesity prevalence (p = 0.007). Comparison between male and female, as well as urban and rural, showed different prevalence of MetS co-morbidities. Abdominal obesity and hypertriglyceridaemia were consistently appeared in all groups, suggesting to play a role as determinant of MetS in both urban and rural.Prevalence of obesity and MetS in urban were two times higher when compared to rural. Abdominal obesity and hypertriglyceridaemia appears to be the key determinant of MetS in both urban and rural Balinese. Our results indicated an association of the ADRB3 Trp64Arg gene polymorphism with obesity in the rural female.Excess bodyweight is one of the most important risk factors contributing to the overall burden of disease worldwide. In 2005, approximately 23.2% adults were classified as overweight, while 9.8% were obese [1]. Average life expectancy was reduced due to the adverse conseque
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