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Search Results: 1 - 10 of 146253 matches for " Su-Fang Wang "
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Identification and Behavioral Evaluation of Sex Pheromone Components of the Chinese Pine Caterpillar Moth, Dendrolimus tabulaeformis
Xiang-Bo Kong, Kui-Wei Liu, Hong-Bin Wang, Su-Fang Zhang, Zhen Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033381
Abstract: Background The Chinese pine caterpillar moth, Dendrolimus tabulaeformis Tsai and Liu (Lepidoptera: Lasiocampidae) is the most important defoliator of coniferous trees in northern China. Outbreaks occur over enormous areas and often lead to the death of forests during 2–3 successive years of defoliation. The sex pheromone of D. tabulaeformis was investigated to define its chemistry and behavioral activity. Methodology/Principal Findings Sex pheromone was collected from calling female D. tabulaeformis by headspace solid phase microextraction (SPME) and by solvent extraction of pheromone glands. Extracts were analyzed by coupled gas chromatography/mass spectrometry (GC-MS) and coupled GC-electroantennographic detection (GC-EAD), using antennae from male moths. Five components from the extracts elicited antennal responses. These compounds were identified by a combination of retention indices, electron impact mass spectral matches, and derivatization as (Z)-5-dodecenyl acetate (Z5-12:OAc), (Z)-5-dodecenyl alcohol (Z5-12:OH), (5Z,7E)-5,7-dodecadien-1-yl acetate (Z5,E7-12:OAc), (5Z,7E)-5,7-dodecadien-1-yl propionate (Z5,E7-12:OPr), and (5Z,7E)-5,7-dodecadien-1-ol (Z5,E7-12:OH). Behavioral assays showed that male D. tabulaeformis strongly discriminated against incomplete and aberrant blend ratios. The correct ratio of Z5,E7-12:OAc, Z5,E7-12:OH, and Z5,E7-12:OPr was essential for optimal upwind flight and source contact. The two monoenes, Z5-12:OAc and Z5-12:OH, alone or binary mixtures, had no effect on behavioral responses when added to the optimal three-component blend. Conclusions/Significance The fact that deviations from the optimal ratio of 100:100:4.5 of Z5,E7-12:OAc, Z5,EZ7-12:OH, and Z5,E7-12:OPr resulted in marked decreases in male responses suggests that biosynthesis of the pheromone components is precisely controlled. The optimal blend of the sex pheromone components of D. tabulaeformis worked out in this study should find immediate use in monitoring this pest in Chinese forests.
Energetics and electronic structure of a single copper atomic chain wrapped in a carbon nanotube: a first-principles study

Zhang Jian-Min,Du Xiu-Juan,Wang Su-Fang,Xu Ke-Wei,

中国物理 B , 2009,
Abstract: In the generalized gradient approximation, the energy and electronic structure are investigated for a single copper atomic chain wrapped in (4, 4), (5, 5) and (6, 6) armchair carbon nanotubes by using the first-principles projector-augmented wave potential within the framework of density functional theory. The results show that the (4, 4) and (5, 5) tubes are too narrow to wrap a Cu chain, but the (6, 6) tube is nearly ideal to wrap a Cu chain on its centre axis. Wider tubes are anticipated to wrap more than one Cu chain spontaneously with forces amounting to a fraction of a nanonewton. Although the tube--chain interaction decreases with the increase of the tube diameter of (4, 4), (5, 5) and (6, 6) successively, the charge density of the Cu@(6, 6) combined system still does not show complete superposition of that of the pristine (6, 6) tube and Cu chain. Successively reducing the restrictions of (4, 4), (5, 5) and (6, 6) tubes on the Cu chain leads to a reduction in shift of the highest peak of the Cu chain towards lower energies, that is from -0.5177~eV of the isolated Cu chain to -1.36785~eV, -0.668~eV and -0.588~eV for the Cu@(4, 4), Cu@(5, 5) and Cu@(6, 6) systems, respectively. In reverse, the strong metallic character of the Cu chain also enhances the metallic character of the combined systems so that the broader pseudogaps of the pristine carbon nanotubes around the Fermi level change into the narrow pseudogaps of the combined systems.
Computational Prediction of Protein-Protein Interactions of Human Tyrosinase
Su-Fang Wang,Sangho Oh,Yue-Xiu Si,Zhi-Jiang Wang,Hong-Yan Han,Jinhyuk Lee,Guo-Ying Qian
Enzyme Research , 2012, DOI: 10.1155/2012/192867
Abstract: The various studies on tyrosinase have recently gained the attention of researchers due to their potential application values and the biological functions. In this study, we predicted the 3D structure of human tyrosinase and simulated the protein-protein interactions between tyrosinase and three binding partners, four and half LIM domains 2 (FHL2), cytochrome b-245 alpha polypeptide (CYBA), and RNA-binding motif protein 9 (RBM9). Our interaction simulations showed significant binding energy scores of ?595.3?kcal/mol for FHL2, ?859.1?kcal/mol for CYBA, and ?821.3?kcal/mol for RBM9. We also investigated the residues of each protein facing toward the predicted site of interaction with tyrosinase. Our computational predictions will be useful for elucidating the protein-protein interactions of tyrosinase and studying its binding mechanisms. 1. Introduction Tyrosinase (EC is ubiquitously distributed in organisms and is a critical enzyme involved in melanin production, with multiple catalytic functions in pigment production [1–3]. Tyrosinase mutations are directly linked to pigmentation disorders in mammals [4, 5] and can cause a browning effect in vegetables [6, 7]. In addition, tyrosinase participates in cuticle formation in insects [8, 9]. In mammals, tyrosinase is a bifunctional enzyme that first converts tyrosine to DOPA and then to DOPA quinone, which is further cyclized and oxidized to produce melanin pigments [10]. The human tyrosinase protein contains two Cu2+-binding sites, two cysteine rich regions, a signal peptide region, a transmembrane anchor domain, and an EGF motif [11]. Two Cu2+ ions in the active site of tyrosinase are coordinated by three histidine residues each and are essential for the enzyme’s catalytic activity [12]. Furthermore, the presence of Cu2+ in the active site of tyrosinase is observed across numerous organisms [13]. Therefore, chelation of tyrosinase Cu2+ by synthetic compounds or agents from natural sources has been targeted as a way to block tyrosinase catalysis for medicinal purposes, darkening problems in agricultural products, and cosmetic interests [14, 15]. As the crystallographic structure of tyrosinase has been gradually elucidated, insights into its catalytic mechanisms and active site have also been revealed [16–18]. However, while the catalytic mechanism of tyrosinase-mediated melanin pigment production has been well studied, the relationship between tyrosinase enzyme activity and protein interactions has not been fully elucidated, despite several reports of interacting proteins for tyrosinase [19–22].
Identification and Analysis of Dry-hot Environment of Yuanmou Using Remotely sensed Images and DEM Data

ZHAO Hong-mei,CHEN Xiao-ling,XIONG Ming,WANG Su-fang,

遥感学报 , 2008,
Abstract: 在干热谷地区的研究中,大都根据海拔高度划定干热谷气候区的范围.而实际上,干热谷景观同时受地形特征(包括高程、坡度和坡向)和人类活动的多重影响.单纯利用高程信息划分干热谷景观区,而没有考虑以上多种因素的影响,显然难以正确地识别不同的自然景观.本文以最为典型的元谋干热谷为例,首先利用多波段混合运算方法提取山体阴影区,从而剔除坡向的影响;然后,以DEM数据为基础,针对陆地卫星影像,分别采用土地利用绘图法、陆面温度阈值法和干热指数法(RTVI,Ratio of Temperature and NDVI)三种方法对干热谷景观进行识别; 最后,利用景观生态学方法对以上识别结果进行对比分析.结果发现,干热指数用于识别干热谷景观区域是最为简单可行的方法; 从研究区域的典型干热谷景观分布特征来看,该景观虽然已远远超出传统高程划分的1350m上限,但是,超出这一上限的干热谷景观斑块相对破碎,水体及植被斑块与干热谷景观斑块交错连接,说明这一区域生态恢复的能力较强.
New discovery about plant defense: plant-plant communication
植物防御的新发现: 植物-植物相互交流

ZHANG Su-Fang,ZHANG Zhen,WANG Hong-Bin,KONG Xiang-Bo,

植物生态学报 , 2012,
Abstract: Plants have developed sophisticated defense systems during their long-time interaction with insects. About three decades ago, it was found that insect-damaged plants can prime their neighbors to express defense proteins, and this phenomenon was called “plant-plant communication”. A series of studies have focused on this topic since then. Results indicate that green-leaf volatiles and terpenes are the main chemicals emitted from the infested plant to the healthy neighbor plants, while direct and indirect defenses of the neighbors may both be regulated. An important consensus about plant-plant communication mechanisms until now is that the volatile organic chemicals do not induce resistance directly, but sensitize the receiver plant for augmented response to subsequent damages, which is called “priming”. However, the molecular mechanism of this phenomenon is unclear. We used Arabidopsis thaliana genome arrays and mutants to examine the molecular mechanisms of plant-plant communication. Our results indicate that several volatiles are effective signals, and the active volatiles are correlated with their emission rhythms to achieve the optimum effect. The ethylene pathway is indispensable for sensing the induction signal in the early phase of induction, while Jasmonic acid signal can amplify the effects. As the mechanisms of plant-plant communication become clearer, future research may focus on the origin and evolution of this phenomenon.
Poly[dimethylammonium [aquadi-μ2-oxalato-dysprosate(III)] trihydrate]
Su-Fang Ye,Hong Lin
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810026140
Abstract: The title compound, {(C2H8N)[Dy(C2O4)2(H2O)]·3H2O}n, was obtained as an unexpected product under hydrothermal conditions. The DyIII atom is chelated by four oxalate anions, two of which are situated on two different centres of inversion. The distorted tricapped trigonal-prismatic coordination sphere of the DyIII atom is completed by a water molecule. The bridging mode of the anions results in the formation of a three-dimensional network with cavities where the ammonium cations and the uncoordinated water molecules reside. The structure is stabilized by numerous N—H...O and O—H...O hydrogen-bonding interactions.
A Novel Process to Prepare Chitosan Macrospheres without Shrinkage and its Application to Immobilize β-Galactosidase
Su-Fang Sun,Yan Zhang
Journal of Chemistry , 2009, DOI: 10.1155/2009/151727
Epstein-Barr Virus BGLF4 Kinase Retards Cellular S-Phase Progression and Induces Chromosomal Abnormality
Yu-Hsin Chang, Chung-Pei Lee, Mei-Tzu Su, Jiin-Tarng Wang, Jen-Yang Chen, Su-Fang Lin, Ching-Hwa Tsai, Min-Jei Hsieh, Kenzo Takada, Mei-Ru Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0039217
Abstract: Epstein-Barr virus (EBV) induces an uncoordinated S-phase-like cellular environment coupled with multiple prophase-like events in cells replicating the virus. The EBV encoded Ser/Thr kinase BGLF4 has been shown to induce premature chromosome condensation through activation of condensin and topoisomerase II and reorganization of the nuclear lamina to facilitate the nuclear egress of nucleocapsids in a pathway mimicking Cdk1. However, the observation that RB is hyperphosphorylated in the presence of BGLF4 raised the possibility that BGLF4 may have a Cdk2-like activity to promote S-phase progression. Here, we investigated the regulatory effects of BGLF4 on cell cycle progression and found that S-phase progression and DNA synthesis were interrupted by BGLF4 in mammalian cells. Expression of BGLF4 did not compensate Cdk1 defects for DNA replication in S. cerevisiae. Using time-lapse microscopy, we found the fate of individual HeLa cells was determined by the expression level of BGLF4. In addition to slight cell growth retardation, BGLF4 elicits abnormal chromosomal structure and micronucleus formation in 293 and NCP-TW01 cells. In Saos-2 cells, BGLF4 induced the hyperphosphorylation of co-transfected RB, while E2F1 was not released from RB-E2F1 complexes. The E2F1 regulated activities of the cyclin D1 and ZBRK1 promoters were suppressed by BGLF4 in a dose dependent manner. Detection with phosphoamino acid specific antibodies revealed that, in addition to Ser780, phosphorylation of the DNA damage-responsive Ser612 on RB was enhanced by BGLF4. Taken together, our study indicates that BGLF4 may directly or indirectly induce a DNA damage signal that eventually interferes with host DNA synthesis and delays S-phase progression.
Effect of Cultural Condition on Fatty Acid Composition of Ralstonia solanacearum

ZHU Yu-Jing,SU Ming-Xing,HUANG Su-Fang,WANG Qiu-Hong,LIU Bo,

微生物学通报 , 2009,
Abstract: 应用气相色谱技术测定不同温度、培养时间、pH值等培养条件下青枯雷尔氏菌(Ralstonia solanacearum)脂肪酸的结果表明: 青枯雷尔氏菌强致病力菌株Rs-J.1.4-010704-01v的脂肪酸种类有14~34种, 主要特征脂肪酸为C16:1ω7c/C15:0 ISO 2OH(10.644 min), C16:0(10.950 min), C18:1ω7c(14.177 min), 所占总百分比含量为总脂肪酸的55.66%~75.69%; 该菌脂肪酸的种类与含量随着培养条件的改变而发生变化,
Regional Homogeneity within the Default Mode Network in Bipolar Depression: A Resting-State Functional Magnetic Resonance Imaging Study
Chun-Hong Liu, Xin Ma, Feng Li, Yong-Jun Wang, Chang-Le Tie, Su-Fang Li, Tao-Lin Chen, Ting-ting Fan, Yu Zhang, Jie Dong, Li Yao, Xia Wu, Chuan-Yue Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048181
Abstract: Aim We sought to use a regional homogeneity (ReHo) approach as an index in resting-state functional magnetic resonance imaging (fMRI) to investigate the features of spontaneous brain activity within the default mode network (DMN) in patients suffering from bipolar depression (BD). Methods Twenty-six patients with BD and 26 gender-, age-, and education-matched healthy subjects participated in the resting-state fMRI scans. We compared the differences in ReHo between the two groups within the DMN and investigated the relationships between sex, age, years of education, disease duration, the Hamilton Rating Scale for Depression (HAMD) total score, and ReHo in regions with significant group differences. Results Our results revealed that bipolar depressed patients had increased ReHo in the left medial frontal gyrus and left inferior parietal lobe compared to healthy controls. No correlations were found between regional ReHo values and sex, age, and clinical features within the BD group. Conclusions Our findings indicate that abnormal brain activity is mainly distributed within prefrontal-limbic circuits, which are believed to be involved in the pathophysiological mechanisms underlying bipolar depression.
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