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Search Results: 1 - 10 of 56355 matches for " Streptococcus suis type 2 "
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Prevalência de Streptococcus suis tipo 2 por meio da técnica de rea??o em cadeia da polimerase em suínos abatidos no Estado do Mato Grosso
Faria, Ana Carolina Silva de;Silva, Maria Cristina da;Oliveira Filho, Jo?o Xavier;Oliveira, Ju?ara Tinasi de;Paula, Daphine Ariadne Jesus de;Chitarra, Cristiane Silva;Nakazato, Luciano;Dutra, Valéria;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782009005000238
Abstract: streptococcus suis is a pathogen that affects the industrial production of swine worldwide. it is extremely important, because it is associated with pigs and humans diseases. the aim of this study was to determine the prevalence of streptococcus suis type 2 in 201 samples of tonsils from clinically healthy animals by the pcr technique. the samples positive for s. suis type 2 were tested for the gene encoding extracellular factors (ef). the results showed that the prevalence (23.38%) was higher than other recent survey in the state, demonstrating that the pcr is a more sensitive method in relation to the bacterial isolation. there was a low occurrence of ef* gene in samples (1.49%) showing great importance to local swine population, because negative strains are potentially less virulent that positive strains.
Adhesion and Invasion of Streptococcus suis Type 2 to Tonsil Epithelial Cells from Neonatal Rabbit

ZENG Qiao-Ying,LU Cheng-Ping,

微生物学报 , 2004,
Abstract: Streptococcus suis type 2 (SS2) is one of the most important zoonotic pathogens world wide. Muramidase released protein (MRP) is a key virulence factor of SS2. In this paper, both SS2 strains, HA9801, naturally with MRP expression, isolated from Jiangsu province and SH006444, without MRP, from Shanghai, were used to study adhesion and invasion of SS2 to tonsil epithelial cells from neonatal rabbit. Adhesion counting assay demonstrated high adhesive levels of both strains. Scanning and transmission electron microscope observation further showed a dense adhering of HA9801 at cellular membrane and microvilli, and HA9801 cells were being internalized by the cell membrane. Lysis counting assay revealed a weak invasion of HA9801 and no invasion of SH006444 to tonsil epithelial cells from neonatal rabbit. Findings of this study suggested, firstly, the tonsil served as the colonizing site and an entrance of SS2 in establishment of infection; secondly, the direct invasion into epithelial cells following their adhesion was one of their mechanisms of breaking through the tonsil epithelial cell barrier.
Identification and Drug Resistance Analysis of Streptococcus suis Type 2 from the Pudong Area

- , 2015,
Abstract: 年03月至08月期间,对来自浦东地区患关节炎的150份病猪和死猪内脏器官及关节液进行细菌分离,对分离细菌进行革兰氏染色、PCR鉴定、药敏实验和多重PCR检测链球菌毒力因子分布(荚膜多糖cps、溶菌酶释放蛋白基因mrp、胞外因子ef、溶血素sly、毒力相关基因orf2、谷氨酸脱氢酶基因gdh、甘油醛-3-磷酸脱氢酶基因gapdh),并对链球菌2型cps基因序列进行进化树分析。结果,从分离到的120株细菌中检测到21株链球菌,分离率为17.5%,2型链球菌分离率为11.67%;链球菌的毒力型呈-/+cps2J-/+mrp-ef-sly-gdh-/+gadph -/+orf2;分离株呈多重耐药性,耐药性为:大环内酯类<硝基呋喃类、氯霉素类、多肽类、氨基糖苷类、四环素类<青霉素类、头孢菌素类、亏诺酮类<林可胺类、磺胺类;cps基因与GenBank上已公布的链球菌2型荚膜多糖基因的核苷酸同源性达94%~99%。
From Mar.to Aug.2014,sick or dead pigs affected with arthrophlogosis from the Pudong area were sampled.The 120 strains of bacteria,isolated from 150 internal organs and joint fluid,were tested by gram stain and multiple PCR methods of detecting virulence factors which include genes about mrp,ef,sly,orf2,gdh and fbps.In addition,the sequence of cps gene of Streptococcus suis type 2 were compared with those of GenBank and a susceptibility testing of isolates were taken.The results showed that the virulence type of 21 strains of Streptococcus (17.5%) and 14 strains of Streptococcus suis type 2 (11.6%) was -/+cps2J-/+mrp-ef-sly-gdh-/+gadph -/+orf2.The 21 strains of Streptococcus showed multi-drug resistance and the strength of drug resistance indicated the following results:Macrolides < Nitrofurans,Fenicols,Methylmercadone,Aminoglycosides,Tetracyclines < Penicillins,Cephalosporins,Quinolones < Lincosamide,Sulfonamides.The identification homology of capsular polysaccharide gene of Streptococcus suis type 2 was 94%~99%.
Cloning,Expression and Animal Experiment of the Immunopotential Fragment of mrp Gene from Streptococcus suis type 2

FAN Hong Jie LU Cheng Ping,TANG Jia Qi,

微生物学报 , 2004,
Abstract: 根据猪链球菌2型(Streptococcus suis type 2)国外分离株的溶菌酶释放蛋白(Muramidasereleased protein, MRP)的基因序列,设计并合成一对引物,利用PCR技术扩增了江苏分离株的开放阅读框298~827bp间529bp的基因片段,并定向克隆至pET32a(+)表达载体中。重组质粒经限制性酶切鉴定和测序,转化至大肠杆菌BL21,经IPTG诱导,可表达分子量约42kD的蛋白。经过镍亲和层析柱层析,获得纯化的重组蛋白。以重组蛋白免疫Balb/c小鼠,以5LD50猪链球菌强毒株攻击后小鼠的相对存活率达625%。证实所表达的MRP片段为重要的保护性抗原。
Identification and detection of trag: a new infection-related gene expressed in vivo from isolates of Streptococcus suis

Haodan Zhu,Hongwei Gu,Chengping Lu,

微生物学报 , 2008,
Abstract: 【目的】trag(transfer gene G)是利用IVIAT(in vivo induced antigen technology)通量筛选鉴定的猪链球菌2型(Streptococcus suis type 2,SS2)感染相关因子,研究该基因在猪链球菌(Streptococcus suis,SS)中的分布情况,研究康复血清与免疫血清在免疫印迹中的反应性有无,间接证明其在体内感染与体外培养时表达差异。【方法】鉴于我国分离株trag与GenBank公布的SS2北美株89/1591的trag序列有95.8%的同源性,据此设计和合成一对检测引物,对SS2我国江苏及四川流行株、其他临床分离株和参考株及SS1、SS1/2、SS9、SS7及C群猪源链球菌共43株进行PCR扩增。另设计一对引物,扩增5株SS代表菌株trag的完整阅读框,并对扩增产物进行测序。据软件分析后,选择TRAG(Transfer protein G?)免疫原性良好的区域片段的核酸设计表达引物,PCR扩增后定向克隆至表达载体pET28a(+)构建表达质粒,表达蛋白转印到PVDF膜上,分别与SS2猪康复血清和猪高免血清反应。【结果】trag在SS2中94%(30/32)阳性,SS9中67%(4/6)阳性,SS7阳性,SS1、SS1/2及C群菌阴性。5株细菌TRAG的氨基酸序列与SS2中国株98HAH33、05ZYH33及北美株89/1591同源性>97%。所获得重组蛋白只能被康复血清识别。【结论】从SS 致病株中检出感染相关基因trag,提示该基因可能与SS 致病性有关,重组蛋白的免疫转印结果表明,TRAG可能与SS2体内感染相关。
Identification and characterization of a novel antigenic protein of Streptococcus suis type 2

ZHANG Wei,WU Zong-fu,LU Cheng-ping,

微生物学报 , 2007,
Abstract: An immunogenic protein, named HM3, of Streptococcus suis type 2 HA9801 was identified by using immunoproteomic assay. Some characters of this protein were analyzed by several online bioinformatical tools, including BLAST, SinglP, HMMTOP and PSORTb. The most homologous sequence of HM3 was extracellular solute-binding protein (gi|69246104) of Enterococcus faecium. The predictions results showed that HM3 contained Signal peptide and one transmembrane region, and Non-Cytoplasmic Localization were also predicted. Partial gene of this protein were amplified from the genome of HA9801 by PCR and inserted into expression plasmid pET-32a ( ) after double digested by BamH I and Sal I, then transformed into BL21 (DE3) where they were induced to express by IPTG. After induced, there was specific proteins band of approximately 45kDa on the SDS-PAGE gel. Western-blotting showed that recombinant protein could react with immune serum of HA9801 of SPF (Specefic pathogen Free)mini-swine. This protein could be taken as a vaccine candidate of SS2.
Putative lipoproteins of Streptococcus suis type 2 identified by bioinformatic genome analysis

Liyun Sun,

微生物学报 , 2008,
Abstract: 目的]鉴别已公布基因组序列的SS2 05ZYH33株的脂蛋白(Lpp).方法]首先从Genbank获取由 SS2 05ZYH33基因组推测的蛋白质氨基酸序列,然后利用Lpp预测软件PrositeScan和DOLOP预测05ZYH33株的Lpp,采用SignalP 3.0 HMM、PrediSi、Phobius、LipoP-HMM和TMHMMversion2.0分析预测的Lpp的信号肽,然后经"多数票决法"确定Lpp;最后利用InterProScan和BlastP服务器对鉴定的Lpp进行功能分析.结果]鉴定出34种Lpp,占SS2致病株05ZYH33蛋白质组的1.555%.结构与功能分析结果表明,最大的一类Lpp是ABC运输蛋白的底物结合蛋白,共有16种,其中YP_001197586、YP_001197918、YP_001198449、YP_001199435、YP_001197482、YP_001199452和YP_001198914等7种基因可与其他成分组成完整的ABC运输蛋白操纵子,这些Lpp在SS2获取糖、氨基酸和金属离子等营养物质方面具有重要作用.YP_001197698与无乳链球菌的黏附素Lmb和化脓链球菌的黏附素Lbp及YP_001198710与肺炎链球菌的SlrA高度同源,推测它们与黏附功能有关,为SS2新的毒力因子;其他Lpp包括酶、参与蛋白质折叠过程的Lpp及功能未知的Lpp.结论]这些资料提示Lpp在SS2的生理和致病性方面具有重要作用.
Fusion and Apoptosis of Epithelial Cells Induced by Muramidase Released Protein of Streptococcus suis Type 2

Zeng Qiaoying,Lu Chengping,

微生物学报 , 2003,
Abstract: The pathogenic role of muramidase released protein (MRP),a virulence factor of Streptococcus suis type 2 (SS2) is poorly understood. The purified MRP was co-incubated with HEp-2 cells to determine the effect of MRP on epithelial cells. Under light microscope, two principal morphologic changes were observed. Firstly, the cells were fused to form syncytia and a apoptosis followed. Secondly, single cell was also induced to apoptosis at high level as 18%, which was verified by transmission electron microscopy and flow cytometry. It showed that MRP alone was capable of a vi- rulence factor of SS2.
Analysis of Virulence-Related Proteins of Streptococcus suis Type 2 from Swine Streptococcus Isolatrd in China

Ou Yu,Lu Chengping,

微生物学报 , 2002,
Abstract: Virulence-related proteins, muraminidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis type 2, were extracted from Jiangsu Isolate HA9801 and were used as antigens for preparation of antibodies. Bacterium cell envelope proteins and extracellular proteins of swine Streptococcus strains including 17 Chinese isolates, 1 German strain and 1 human isolate of Streptococcus suis type 2, were analyzed by SDS-PAGE and immunoblotting using the above antibodies, 11 strains produced MRP and 10 strains possessed EF or EF *. They exist four phenotypes:MRP +EF +(8/19), MRP +EF *(1/19),MRP +EF -(1/19),MRP -EF -(10/19).
Found of Putative Virulent Genes of Streptotoccus suis Type 2 Strains

TIAN Yun~,

微生物学报 , 2004,
Abstract: 猪链球菌2型(SS2)感染已成为影响全世界养猪业的重要问题之一。SS2菌株可分为毒力株、弱毒力株和无毒力株,但目前尚无区分此3类菌株的快速、有效的检测方法。为了获得毒力株特异的基因序列,对毒力株HA9801及无毒力株12^#进行了抑制性差减杂交(SSH)实验,获得了5个可能的新的毒力基因片段,分别是转录调节子、氨基酸通透酶、ABC转运子及表面锚定蛋白,在国内外尚属首次报道。这一发现将有助于区分SS2型菌株的毒力类型,并为SS2毒力株检测方法的建立奠定基础。
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