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Search Results: 1 - 10 of 859 matches for " Stankovi? Slavica "
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Genetic variability of maize pathogens in Serbia
StankoviSlavica,Levi? Jelena,Ivanovi? Dragica
Genetika , 2007, DOI: 10.2298/gensr0702227s
Abstract: Variability of some maize pathogens was identified in the last 50 years of research in Serbia, mostly by their cultural characteristics and susceptibility of test genotypes and only in some cases by determination of mating types, vegetative compatibility or biochemical methods. Although more advanced methods that can determine within population variability at the molecular level were developed, they are still not applied in research in Serbia. The highest variability was determined for maize leaf pathogens - Exserohilum turcicum (Pass.) Leonard & Suggs (2 races) and Bipolaris zeicola (Stout) Shoemaker (2 races), although this variability is significantly lower than the variability of the same pathogens found in the world. Researches conducted with the aim to determine mating types and vegetative compatibility of the Fusarium species, a maize root and stalk pathogen, indicated their high variability in Serbia. Considering the pathogen ability to adapt easily and quickly to new genotypes, agro-ecological conditions and crop practice, a constant surveillance of parasite divergence and epidemiology is necessary in order to avoid detrimental consequences on maize yield and quality.
The determination of Exserohilum turcicum virulence factors in Serbia
Levi? Jelena,StankoviSlavica,Petrovi? Tijana
Genetika , 2008, DOI: 10.2298/gensr0803271l
Abstract: The determination of Exserohilum turcicum virulence factors and resistance responses of three sets of maize inbred lines (four differential, eight isogenic and 22 commercial inbreeds) to three isolates of this pathogen under greenhouse conditions were studied. The maize inbreeds were selected according to previous testing of resistance based on lesion types in 194 inbreeds under field conditions of plant inoculation with the E. turcicum race 0 (designated as the isolate MRI-Et). The standard procedure was applied to obtained isolates MRIZP-1747 and MRIZP-1416 from resistant and susceptible lesion types, respectively. These lesions were developed on the same leaf of a plant of the experimental hybrid no. 163/99 grown in a nursery at Zemun Polje during 1999. The third isolate (MRIZP-1435) was isolated from a leaf sample originating from the location of Srbobran in which the occurrence of northern corn leaf blight (NCLB), caused by Exserohilum turcicum, was intensive. Based upon virulence/avirulence of three isolates of E. turcicum on differential maize inbred lines, it was found out that the isolate MRIZP-1747 could be classified as race 0, whereas isolates MRIZP-1416 and MRIZP-1435 could be classified as race 1. These are the first results that confirm the presence of race 1 of E. turcicum in Serbia. Not including differential lines, 22 and six lines were resistant to race 0 and race 1, respectively, while eight and five lines were resistant and susceptible to both races, respectively. All isogenic lines not containing the Ht gene were susceptible to both races 0 and 1.
Variability of Pathogenicity of Fusarium spp. Originating from Maize and Wheat Grains
Sonja Tan?i?,Slavica Stankovi,Jelena Levi?
Pesticidi i Fitomedicina , 2009,
Abstract: Differences in the pathogenicity of 93 isolates of seven species belonging to the genus Fusarium (F. graminearum, F. verticillioides, F. proliferatum, F. subglutinans, F. sporotrichioides, F. semitectum and F. equiseti), originating from maize kernels (61) and wheat grains (32), were examined based on the germination percentage of inoculated seeds. The studied species demonstrated inter- and intraspecies variability regarding the effects on maize seed germination. On the average, the greatest germination reduction was found in seeds inoculated with the spore suspensions of F. sporotrichioides and F. graminearum. A similar reduction was detected in seeds inoculated with F. proliferatum and F. subglutinans. The effect of F. subglutinans on seed germination reduction was higher compared to the two latter species, while the effects of F. semitectum and F. equiseti were smallest. The majority of isolates were of moderate pathogenicity, while the lowest number of isolates was either very pathogenic (7) or apathogenic (10). Pathogenicity of the isolates originating from wheat grains was generally lower than the pathogenicity of isolates originating from maize kernels, with the exception of F. sporotrichioides.
Dietary magnesium intake and coronary heart disease risk: A study from Serbia
Slavica Stevanovi?,Maja Nikoli?,Aleksandra Stankovi
Medicinski Glasnik , 2011,
Abstract: Aim To assess the relationship between dietary magnesium intakeand the risk of coronary heart disease (CHD). Methods A conducted case-control study included 290 randomly selected cases (mean age 59.98 +/- 10.03 years) with first event of an acute coronary syndrome and 290 selected controls paired by sex, age and region (mean age 59.43 +/- 10.10 years) admitted to the same hospitals without any suspicion of coronary disease. A diet was assessed by an interviewer-administered semi-quantitative food frequency questionnaire, and magnesium intake was derived from the nutrient database. Results Subjects with coronary heart disease had significantly lower intake of foods containing high levels of magnesium like whole grain (p<0.0001), legumes (p<0.05) and nuts (p<0.05). Lower dietary magnesium intake was found to be positively associated with risk of coronary heart disease (0.027). Conclusion Our findings suggest that dietary intake of magnesium was associated with reduced risk of coronary heart disease among Serbian population.
On-line blind separation of non-stationary signals
Todorovi?-Zarkula Slavica,Todorovi? Branimir,Stankovi? Miomir
Yugoslav Journal of Operations Research , 2005, DOI: 10.2298/yjor0501079t
Abstract: This paper addresses the problem of blind separation of non-stationary signals. We introduce an on-line separating algorithm for estimation of independent source signals using the assumption of non-stationary of sources. As a separating model, we apply a self-organizing neural network with lateral connections, and define a contrast function based on correlation of the network outputs. A separating algorithm for adaptation of the network weights is derived using the state-space model of the network dynamics, and the extended Kalman filter. Simulation results obtained in blind separation of artificial and real-world signals from their artificial mixtures have shown that separating algorithm based on the extended Kalman filter outperforms stochastic gradient based algorithm both in convergence speed and estimation accuracy.
Identification of Tilletia species using rep-PCR fingerprinting technique
?upunski Vesna,Ignjatovi?-Mici? Dragana,Nikoli? Ana,StankoviSlavica
Genetika , 2011, DOI: 10.2298/gensr1101183z
Abstract: Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.
Diversity of the Fusarium verticillioides and F. proliferatum isolates according to their fumonisin B1 production potential and origin
Tan?i? Sonja,StankoviSlavica,Levi? Jelena,Krnjaja Vesna
Genetika , 2012, DOI: 10.2298/gensr1201163t
Abstract: Species of the genus Fusarium are characterised by the exceptional intraspecies and interspecies variability in respect to morphological, physiological and genetic properties. Intraspecies and interspecies diversity of Fusarium verticillioides and Fusarium proliferatum isolates in the production of fumonisin B1 according to their origin from maize and wheat grains was studied. Fumonisin B1 production potential of investigated 42 isolates was assessed by HPTLC and ELISA method. All 22 and 20 investigated F. verticillioides and F. proliferatum isolates, respectively, had the ability to produce fumonisin B1 toxin. Fumonisin B1 production potential of F. verticillioides and F. proliferatum varied from 88.60 ppm to 1,300.60 ppm and from 2.37 ppm to 1,246.00 ppm, respectively. According to Mann-Whitnev U test, there were no significant differences between the fumonisin B1 production mean values of isolates of F. verticillioides originated from maize and wheat (628.13 ppm and 696.38 ppm, respectively), as well as, fumonisin B1 production mean values of F. proliferatum isolates (212.32 ppm and 158.07 ppm, respectively). Variability coefficient values indicated that fumonisin B1 production potential variability was more expressed in maize than wheat originated isolates for both species.
Fusariotoxins in Wheat Grain in Serbia
Ana Stepani?,Slavica Stankovi,Jelena Levi?,Mirko Ivanovi?
Pesticidi i Fitomedicina , 2011,
Abstract: Samples of wheat grain (41), collected during the 2010 harvest from seven localities inSerbia, were analysed for the presence of zearalenone (ZEA), T-2 toxin, deoxynivalenol (DON)and fumonisine B1 (FB1). Results of Enzyme-Linked Immunosorbent Assay (ELISA) showedthat all analysed samples were positive for the presence of at least one of four observedfusariotoxins. The most distributed mycotoxins were ZEA (90.2%, with the average concentrationof 442.6μg kg–1) and T-2 (90.2%, with the average concentration of 24.2 μg kg–1).DON (73.2%) and FB1 (84.4%) were detected in a somewhat smaller number of samples, buttheir average concentrations were higher (1988.1 μg DON kg–1 and 882.7 μg FB1 kg–1). Theestablished correlations between concentrations of DON and FB1 (r = 0.32) or DON and ZEA(r = 0.22) were not statistically significant. A negative correlation was established betweenconcentrations of T-2 and FB1 (r= -0.24), as well as, between T-2 and DON (r = -0.36). Detectedconcentrations of ZEA and T-2 were bellow the level prescribed by the World Health Organisation(WHO), while concentrations of FB1 and DON detected in five that is, 17 samples,respectively, were above the permissible limit for human consumption
Nasal Carriage of Staphylococcus aureus in Healthy Adults and in School Children
Marina Dini?,Slavica Vukovi?,Branislava Koci?,Dobrila Stankovi? ?or?evi?
Acta Facultatis Medicae Naissensis , 2013, DOI: 10.2478/v10283-012-0033-3
Abstract: Staphylococcus aureus (S. aureus) is a microorganism that colonizes the skin and mucosal surfaces of healthy individuals, but it is also one of the most common causes of community-acquired and hospital infections. Nasal carriage of S. aureus represents a major risk factor for the development of infection with this bacterium. A special therapeutic problem are methicillin-resistant isolates of S. aureus (MRSA). The aim of this study was to assess the nasal carriage of S. aureus in healthy individuals in the local community, and the sensitivity of the microorganism to antibiotics. The study enrolled 56.868 healthy individuals aged 19 to 65 years, and 2.040 healthy school children aged 15 to 19 years. Specimens to be studied were obtained from anterior nares. We used the disk diffusion method (Kirby-Bauer) on Mueller-Hinton agar to assess the sensitivity of isolated S. aureus. S. aureus was isolated in 1.381 (2.34%) respondents. Positive findings were obtained in 2.33% of adult examinees, and in 2.59% of studied school children. We found a low level of susceptibility only to penicillin (5.36%). The susceptibility of S. aureus isolates to all other tested antibiotics was present in a high percentage, with the lowest percentage of susceptibility to doxycycline (71.54%) and erythromycin (86.09%). The highest percentage of susceptibility of tested isolates was reported for fusidic acid (99.27%). In relation to the total number of S. aureus isolates from nasal swabs in adults, MRSA was present in 8.96% (119 isolates), while there were 4 MRSA isolates from nasal swabs in school children. In this study, we established a low percentage of nasal carriage of S. aureus in the population of healthy individuals, but a high percentage of MRSA.
Effects of Metribuzin on the Activity of Some Enzymes in Soil
Ljiljana ?antri?,Ljiljana Radivojevi?,Slavica Ga?i?,Radmila Stankovi?-Kalezi?
Pesticidi i Fitomedicina , 2008,
Abstract: Eeffects of metribuzin on the activity of some enzymes in soil was investigated. Trials were set up in the laboratory on a clay loam soil. Metribuzin was applied at 12.0, 24.0 and 60.0 mg/kg soil rates and soil samples were collected 3, 7, 15, 30 and 45 days after metribuzin treatment for analyses. Alkaline phosphatase, acid phosphatase, dehydrogenase, urease and β-glucosidase were recorded.The results showed that the intensity of metribuzin effects on the activity of enzymes depended on treatment rate, exposure time and enzyme group. Metribuzin had an inhibiting effect on acid phosphatese and dehydrogenase, as well as on alkaline phosphatase inthe initial stage before it turned into a stimulating one, while metribuzin stimulated urease and had no effect on β-glucosidase.
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