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Search Results: 1 - 10 of 131285 matches for " Stanislav V. Sosnovtsev "
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Polypyrimidine Tract Binding Protein Functions as a Negative Regulator of Feline Calicivirus Translation
Ioannis Karakasiliotis,Surender Vashist,Dalan Bailey,Eugenio J. Abente,Kim Y. Green,Lisa O. Roberts,Stanislav V. Sosnovtsev,Ian G. Goodfellow
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009562
Abstract: Positive strand RNA viruses rely heavily on host cell RNA binding proteins for various aspects of their life cycle. Such proteins interact with sequences usually present at the 5′ or 3′ extremities of the viral RNA genome, to regulate viral translation and/or replication. We have previously reported that the well characterized host RNA binding protein polypyrimidine tract binding protein (PTB) interacts with the 5′end of the feline calicivirus (FCV) genomic and subgenomic RNAs, playing a role in the FCV life cycle.
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid
Gabriel I. Parra, JoLynn Azure, Renate Fischer, Karin Bok, Carlos Sandoval-Jaime, Stanislav V. Sosnovtsev, Peter Sander, Kim Y. Green
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067592
Abstract: Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52–56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses.
Diversity of Murine Norovirus Strains Isolated from Asymptomatic Mice of Different Genetic Backgrounds within a Single U.S. Research Institute
Elyssa L. Barron, Stanislav V. Sosnovtsev, Karin Bok, Victor Prikhodko, Carlos Sandoval-Jaime, Crystal R. Rhodes, Kim Hasenkrug, Aaron B. Carmody, Jerrold M. Ward, Kathy Perdue, Kim Y. Green
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0021435
Abstract: Antibody prevalence studies in laboratory mice indicate that murine norovirus (MNV) infections are common, but the natural history of these viruses has not been fully established. This study examined the extent of genetic diversity of murine noroviruses isolated from healthy laboratory mice housed in multiple animal facilities within a single, large research institute- the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (NIAID-NIH) in Bethesda, Maryland, U.S. Ten distinct murine norovirus strains were isolated from various tissues and feces of asymptomatic wild type sentinel mice as well as asymptomatic immunodeficient (RAG 2?/?) mice. The NIH MNV isolates showed little cytopathic effect in permissive RAW264.7 cells in early passages, but all isolates examined could be adapted to efficient growth in cell culture by serial passage. The viruses, although closely related in genome sequence, were distinguishable from each other according to facility location, likely due to the introduction of new viruses into each facility from separate sources or vendors at different times. Our study indicates that the murine noroviruses are widespread in these animal facilities, despite rigorous guidelines for animal care and maintenance.
Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages
Christiane E. Wobus,Stephanie M. Karst,Larissa B. Thackray,Kyeong-Ok Chang,Stanislav V. Sosnovtsev,Ga?l Belliot,Anne Krug,Jason M. Mackenzie,Kim Y. Green,Herbert W. Virgin IV
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0020432
Abstract: Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology.
Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages
Christiane E Wobus,Stephanie M Karst,Larissa B Thackray,Kyeong-Ok Chang,Stanislav V Sosnovtsev,Ga?l Belliot,Anne Krug,Jason M Mackenzie,Kim Y Green,Herbert W. Virgin IV
PLOS Biology , 2004, DOI: 10.1371/journal.pbio.0020432
Abstract: Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology.
Optical Rotation of Linearly Polarized Light Propagating through a Nonideal 1D-Superlattice  [PDF]
Vladimir V. Rumyantsev, Stanislav A. Fedorov
Materials Sciences and Applications (MSA) , 2010, DOI: 10.4236/msa.2010.11006
Abstract: The problem of finding polariton modes (necessary for calculating gyrotropic characteristics) in space-dispersed superlattices is not yet solved. At the same time the specified quantities can be approximately evaluated if the widths of layers comprising a multilayer material are much bigger then the characteristic scales of space dispersion. In such a case the contribution of individual layers to gyrotropy can be regarded as independed. Thus the corresponding optical quantities can be expressed through the layers' gyrotropic characteristics. This approach is applied to calculate the specific rotation angle of plane of polarization of light propagating through a nonideal 1D-superlattice, which varies in composition as well as in layers' width.
Frequency-Concentration Dependence of Optical Activity of a Non-Ideal 1D-Superlattice  [PDF]
Vladimir V. Rumyantsev, Stanislav A Fedorov
Journal of Modern Physics (JMP) , 2011, DOI: 10.4236/jmp.2011.212192
Abstract: In the present work the widths of layers constituting the non-ideal superlattice are much bigger then the characteristic scales of space dispersion. In such a case the contribution of individual layers to gyrotropy can be regarded as independed. Thus the corresponding optical quantities can be expressed through the layers’ gyrotropic characteristics. This approach is applied to calculate the specific rotation angle of plane of polarization of light propagating through a nonideal 1D-superlattice, which varies in composition as well as in layers’ width. We carry out numerical calculation of the frequency dispersion of optical activity of a non-ideal superlattice, which includes impurity layers with point defects.
Organization-economic mechanizm of forming innovative systems (health-resort sector case-study)
Stanislav V. Koshcheev
European Researcher , 2011,
Abstract: The analysis of recreation and tourism complex innovative system was carried out. It showed the availability of all necessary conditions for the formation of innovative route development and creation of modern, competitive recreation services.
Formation of innovative clusters in tourism
Stanislav V. Koshcheev
European Researcher , 2011,
Abstract: Modern preconditions of development innovative clusters in Russian economy are analyzed. The importance of application of cluster approach to management of innovations in tourism is shown. Necessity formation of innovative clusters in region of tourist specialization for increase of competitiveness of territory is reflected. The model of formation of innovative clusters in tourism is offered.
Increase of Authenticity of Assessment of Expenditures Повышение достоверности оценок расходов
Vashchilin Stanislav V.
Business Inform , 2013,
Abstract: The article studies the issue of increase of authenticity of assessment of expenditures. It proves that both market and non-market factors influence it. Market factors mainly regulate the composition and level of individual expenditure elements. They are: regulatory and legal, competitive and infrastructure. Non-market factors establish the order of formation of cost of an individual expenditure object. They are: object of assessment, accounting methods, methods of writing-off reserves on their retirement, methods of charging depreciation, forms and systems of payment for labour, base of distribution of indirect expenditures, methods of assessment of accompanying products, methods of distribution of expenditures between individual products in complex productions, sequence of formation of cost of individual objects of expenditures. В статье исследуется вопрос повышения достоверности оценки расходов. Доказано, что на нее оказывают влияние как рыночные так и не рыночные факторы. Рыночные факторы в основном регламентируют состав и уровень отдельных элементов расходов. К ним относятся: нормативно-правовые, конкурентные и инфраструктурные. Не рыночные факторы устанавливают порядок формирования себестоимости отдельного объекта расходов. К ним относятся: объект оценки, методы учета, методы списания запасов при выбытии, методы начисления амортизации, формы и системы оплаты труда, база распределения косвенных расходов, методы оценки попутной продукции, методы распределения расходов между отдельными продуктами в комплексных производствах, последовательность формирования себестоимости отдельных объектов расходов.
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